Jundishapur Journal of Microbiology

Published by: Kowsar

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Mehrdad Zalipour 1 , Hadi Sedigh Ebrahim-Saraie 1 , Jamal Sarvari 1 and Reza Khashei 1 , *
Authors Information
1 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: December 01, 2016, 9 (12); e41431
  • Published Online: November 27, 2016
  • Article Type: Research Article
  • Received: August 10, 2016
  • Revised: October 31, 2016
  • Accepted: November 19, 2016
  • DOI: 10.5812/jjm.41431

To Cite: Zalipour M, Sedigh Ebrahim-Saraie H, Sarvari J, Khashei R. Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran, Jundishapur J Microbiol. 2016 ; 9(12):e41431. doi: 10.5812/jjm.41431.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Otto M. Staphylococcus colonization of the skin and antimicrobial peptides. Expert Rev Dermatol. 2010; 5(2): 183-95[DOI][PubMed]
  • 2. Nejabat M, Khashei R, Bazargani A, Sedigh Ebrahim-Saraie H, Motamedifar M. Evaluation of High-Level of Mupirocin Resistance among Clinical Isolates of Methicillin-Resistant Staphylococcus aureus from Shiraz, Iran (2008-2009). Pharm Sci. 2015; 21(4): 225-8[DOI]
  • 3. Khashei R, Sedigh HSE, Hosseini Alfatemi M, Zomorodian K. Antimicrobial resistance patterns of colonizing microflora on the personnel hands and noses working in the Neonatal Intensive Care Unit (NICU). World Appl Sci J. 2014; 30(10): 1232-7[DOI]
  • 4. Zomorodian K, Rahimi MJ, Taheri M, Ghanbari Asad A, Khani S, Ahrari I, et al. The cutaneous bacterial microflora of the bodybuilders using anabolic-androgenic steroids. Jundishapur J Microbiol. 2015; 8(1)[DOI][PubMed]
  • 5. Ebrahim-Saraie HS, Motamedifar M, Sarvari J, Hoseini Alfatemi SM. Emergence of SCCmec Type I Obtained From Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran. Jundishapur J Microbiol. 2015; 8(6)[DOI][PubMed]
  • 6. Archer NK, Mazaitis MJ, Costerton JW, Leid JG, Powers ME, Shirtliff ME. Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence. 2011; 2(5): 445-59[DOI][PubMed]
  • 7. Osman KM, Abd El-Razik KA, Marie HS, Arafa A. Relevance of biofilm formation and virulence of different species of coagulase-negative staphylococci to public health. Eur J Clin Microbiol Infect Dis. 2015; 34(10): 2009-16[DOI][PubMed]
  • 8. Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010; 5(6): 917-33[DOI][PubMed]
  • 9. Otto M. Staphylococcal biofilms. Curr Top Microbiol Immunol. 2008; 322: 207-28[PubMed]
  • 10. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002; 15(2): 167-93[PubMed]
  • 11. Solati SM, Tajbakhsh E, Khamesipour F, Gugnani HC. Prevalence of virulence genes of biofilm producing strains of Staphylococcus epidermidis isolated from clinical samples in Iran. AMB Express. 2015; 5(1): 134[DOI][PubMed]
  • 12. Arciola CR, Campoccia D, Ravaioli S, Montanaro L. Polysaccharide intercellular adhesin in biofilm: structural and regulatory aspects. Front Cell Infect Microbiol. 2015; 5: 7[DOI][PubMed]
  • 13. Pedroso SH, Sandes SH, Luiz KC, Dias RS, Filho RA, Serufo JC, et al. Biofilm and toxin profile: A phenotypic and genotypic characterization of coagulase-negative staphylococci isolated from human bloodstream infections. Microb Pathog. 2016; 100: 312-8[DOI][PubMed]
  • 14. Namvar AE, Asghari B, Ezzatifar F, Azizi G, Lari AR. Detection of the intercellular adhesion gene cluster (ica) in clinical Staphylococcus aureus isolates. GMS Hyg Infect Control. 2013; 8(1)[DOI][PubMed]
  • 15. Cue D, Lei MG, Lee CY. Genetic regulation of the intercellular adhesion locus in staphylococci. Front Cell Infect Microbiol. 2012; 2: 38[DOI][PubMed]
  • 16. Pokrovskaya V, Poloczek J, Little DJ, Griffiths H, Howell PL, Nitz M. Functional characterization of Staphylococcus epidermidis IcaB, a de-N-acetylase important for biofilm formation. Biochemistry. 2013; 52(32): 5463-71[DOI][PubMed]
  • 17. Performance Standards for Antimicrobial Susceptibility Testing; 25th Informational Supplement. M100-S25. 2015;
  • 18. Sambrook J, Russell DW. Purification of nucleic acids by extraction with phenol:chloroform. CSH Protoc. 2006; 2006(1)[DOI][PubMed]
  • 19. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005; 43(10): 5026-33[DOI][PubMed]
  • 20. Arciola CR, Baldassarri L, Montanaro L. Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. J Clin Microbiol. 2001; 39(6): 2151-6[DOI][PubMed]
  • 21. Oliveira A, Cunha Mde L. Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci. BMC Res Notes. 2010; 3: 260[DOI][PubMed]
  • 22. Eftekhar F, Dadaei T. Biofilm formation and detection of icaAB genes in clinical isolates of methicillin resistant Staphylococcus aureus. Iran J Basic Med Sci. 2011; 14(2): 132-6
  • 23. Ohadian Moghadam S, Pourmand MR, Aminharati F. Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran. J Infect Dev Ctries. 2014; 8(12): 1511-7[DOI][PubMed]
  • 24. Rezaei M, Moniri R, Mousavi SGA, Jabari Shiade M. Prevalence of Biofilm Formation Among Methicillin Resistance Staphylococcus aureus Isolated From Nasal Carriers. Jundishapur J Microbiol. 2013; 6(6)[DOI]
  • 25. Jain A, Agarwal A. Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci. J Microbiol Methods. 2009; 76(1): 88-92[DOI][PubMed]
  • 26. Nasr RA, AbuShady HM, Hussein HS. Biofilm formation and presence of icaAD gene in clinical isolates of staphylococci. Egypt J Med Human Genetics. 2012; 13(3): 269-74[DOI]
  • 27. Arciola CR, Campoccia D, Gamberini S, Donati ME, Pirini V, Visai L, et al. Antibiotic resistance in exopolysaccharide-forming Staphylococcus epidermidis clinical isolates from orthopaedic implant infections. Biomaterials. 2005; 26(33): 6530-5[DOI][PubMed]
  • 28. Petrelli D, Repetto A, D'Ercole S, Rombini S, Ripa S, Prenna M, et al. Analysis of meticillin-susceptible and meticillin-resistant biofilm-forming Staphylococcus aureus from catheter infections isolated in a large Italian hospital. J Med Microbiol. 2008; 57: 364-72[DOI][PubMed]
  • 29. Yazdani R, Oshaghi M, Havayi A, Pishva E, Salehi R, Sadeghizadeh M, et al. Detection of icaAD gene and biofilm formation in Staphylococcus aureus isolates from wound infections. Iran J Public Health. 2006; 35(2): 25-8
  • 30. Taj Y, Essa F, Aziz F, Kazmi SU. Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. J Infect Dev Ctries. 2012; 6(5): 403-9[PubMed]
  • 31. Aoki EE, Pizzolitto AC, Garcia LB, Pizzolitto EL. Stpahylococcus aureus biofilms on central venous haemodialysis catheters. Braz J Microbiol. 2005; 36(4): 342-6[DOI]
  • 32. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. J Glob Antimicrob Resist. 2016; 7: 110-3[DOI][PubMed]
  • 33. Sahal G, Bilkay IS. Multi drug resistance in strong biofilm forming clinical isolates of Staphylococcus epidermidis. Braz J Microbiol. 2014; 45(2): 539-44[PubMed]
  • 34. Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LT, Hamat RA, Karunanidhi A, et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. J Biomed Biotechnol. 2012; 2012: 976972[DOI][PubMed]
  • 35. Rohde H, Burandt EC, Siemssen N, Frommelt L, Burdelski C, Wurster S, et al. Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections. Biomaterials. 2007; 28(9): 1711-20[DOI][PubMed]
  • 36. Darwish SF, Asfour HA. Investigation of biofilm forming ability in Staphylococci causing bovine mastitis using phenotypic and genotypic assays. ScientificWorldJournal. 2013; 2013: 378492[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments