Jundishapur Journal of Microbiology

Published by: Kowsar

Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq

Aram Khezri 1 , 2 , * , Esmaeel Fallah 1 , 3 , 4 , Mostafa Mostafazadeh 2 , 5 , Adel Spotin 1 , Abbas Shahbazi 1 , Mahmoud Mahami-Oskouei 1 and Taimuor Hazratian 1
Authors Information
1 Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Laboratory Medicine, Ardabil Medical Sciences Branch, Islamic Azad University, Ardabil, Iran
5 Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2016, 9 (11); e38481
  • Published Online: October 18, 2016
  • Article Type: Research Article
  • Received: April 15, 2016
  • Revised: September 25, 2016
  • Accepted: October 8, 2016
  • DOI: 10.5812/jjm.38481

To Cite: Khezri A, Fallah E, Mostafazadeh M, Spotin A, Shahbazi A, et al. Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq, Jundishapur J Microbiol. 2016 ; 9(11):e38481. doi: 10.5812/jjm.38481.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Magnet A, Galvan AL, Fenoy S, Izquierdo F, Rueda C, Fernandez Vadillo C, et al. Molecular characterization of Acanthamoeba isolated in water treatment plants and comparison with clinical isolates. Parasitol Res. 2012; 111(1): 383-92[DOI][PubMed]
  • 2. Lorenzo-Morales J, Ortega-Rivas A, Martinez E, Khoubbane M, Artigas P, Periago MV, et al. Acanthamoeba isolates belonging to T1, T2, T3, T4 and T7 genotypes from environmental freshwater samples in the Nile Delta region, Egypt. Acta Trop. 2006; 100(1-2): 63-9[DOI][PubMed]
  • 3. Chen CJ, Yu MW, Liaw YF. Epidemiological characteristics and risk factors of hepatocellular carcinoma. J Gastroenterol Hepatol. 1997; 12(9-10)-308[PubMed]
  • 4. Bagheri H, Shafiei R, Shafiei F, Sajjadi S. Isolation of acanthamoeba spp. From drinking waters in several hospitals of iran. Iran J Parasitol. 2010; 5(2): 19-25[PubMed]
  • 5. Huang SW, Hsu BM. Isolation and identification of Acanthamoeba from Taiwan spring recreation areas using culture enrichment combined with PCR. Acta Trop. 2010; 115(3): 282-7[DOI][PubMed]
  • 6. Ashford JW, Coburn KL, Fuster JM. The elgiloy microelectrode: fabrication techniques and characteristics. J Neurosci Methods. 1985; 14(4): 247-52[PubMed]
  • 7. Chappell CL, Wright JA, Coletta M, Newsome AL. Standardized method of measuring acanthamoeba antibodies in sera from healthy human subjects. Clin Diagn Lab Immunol. 2001; 8(4): 724-30[DOI][PubMed]
  • 8. Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Martinez E, Valladares B. Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitol Res. 2005; 95(4): 273-7[DOI][PubMed]
  • 9. Khan NA. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev. 2006; 30(4): 564-95[DOI][PubMed]
  • 10. Maghsood AH, Sissons J, Rezaian M, Nolder D, Warhurst D, Khan NA. Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. J Med Microbiol. 2005; 54: 755-9[DOI][PubMed]
  • 11. Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol. 2004; 34(9): 1001-27[DOI][PubMed]
  • 12. Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukaryot Microbiol. 1998; 45(1): 45-54[PubMed]
  • 13. Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Mohebali M, Maghsood AH, et al. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Exp Parasitol. 2009; 121(3): 242-5[DOI][PubMed]
  • 14. Pussard M, Pons R. Morphology of cystic wall and taxonomy of genus Acanthamoeba (Protozoa, Amoebida). Protistologica. 1977; 13(4): 557-98
  • 15. Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol. 2007; 50(1): 1-26[DOI][PubMed]
  • 16. Hooshyar H, Hosseinbigi B, Saraei M, Alizadeh S, Eftakhar M, Rasti S, et al. Genotyping of acanthamoeba isolated from surface and stagnant waters of qazvin, central iran. Iran Red Crescent Med J. 2013; 15(6): 536-8[DOI][PubMed]
  • 17. Nazar M, Haghighi A, Niyyati M, Eftekhar M, Tahvildar-Biderouni F, Taghipour N, et al. Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. J Water Health. 2011; 9(3): 603-8[DOI][PubMed]
  • 18. Page FC. A revised classification of the Gymnamoebia (Protozoa: Sarcodina). Zool J Linn Soc. 1976; 58(1): 61-77
  • 19. Booton GC, Visvesvara GS, Byers T, Kelly DJ, Fuerst PA. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. J Clin Microbiol. 2005; 43(4): 1689-93[DOI][PubMed]
  • 20. Boggild AK, Martin DS, Lee T, Yu B, Low DE. Laboratory diagnosis of amoebic keratitis: comparison of four diagnostic methods for different types of clinical specimens. J Clin Microbiol. 2009; 47(5): 1314-8[DOI]
  • 21. Rezaeian M, Niyyati M, Farnia SH, Haghi AM. Isolation of Acanthamoeba spp. from different environmental sources. Iranian J Parasitol. 2008; 3(1): 44-7
  • 22. Jeong HJ, Yu HS. The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea. Korean J Parasitol. 2005; 43(2): 47-50[PubMed]
  • 23. Kilvington S, Gray T, Dart J, Morlet N, Beeching JR, Frazer DG, et al. Acanthamoeba keratitis: the role of domestic tap water contamination in the United Kingdom. Invest Ophthalmol Vis Sci. 2004; 45(1): 165-9[PubMed]
  • 24. Joslin CE, Tu EY, McMahon TT, Passaro DJ, Stayner LT, Sugar J. Epidemiological characteristics of a Chicago-area Acanthamoeba keratitis outbreak. Am J Ophthalmol. 2006; 142(2): 212-7[DOI][PubMed]
  • 25. Behniafar H, Niyyati M, Lasjerdi Z. Molecular Characterization of Pathogenic Acanthamoeba Isolated from Drinking and Recreational water in East Azerbaijan, Northwest Iran. Environ Health Insights. 2015; 9: 7-12[DOI][PubMed]
  • 26. Ettinger MR, Webb SR, Harris SA, McIninch SP, C. Garman G , Brown BL. Distribution of free-living amoebae in James River, Virginia, USA. Parasitol Res. 2003; 89(1): 6-15[DOI][PubMed]
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