Jundishapur Journal of Microbiology

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Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis

Mohamed Amine Mekni 1 , * , Wafa Achour 2 and Assia Ben Hassen 3
Authors Information
1 Service des Laboratoires - Centre National de Greffe de Moelle osseuse (C.N.G.M.O.) Bab Saadoun 1006 Tunis, Tunisia
2 Professor Associate in the Medicine Faculty of Tunis, Centre National de Greffe de Moelle Osseuse Rue Jebel Lakhdhar Bab Saadoun, 1006 Tunis, Tunisie
3 Professor in the Medicine Faculty of Tunis, Centre National de Greffe de Moelle Osseuse Rue Jebel Lakhdhar Bab Saadoun, 1006 Tunis, Tunisie
Article information
  • Jundishapur Journal of Microbiology: January 01, 2017, 10 (1); e36008
  • Published Online: December 6, 2016
  • Article Type: Research Article
  • Received: January 7, 2016
  • Revised: June 23, 2016
  • Accepted: November 26, 2016
  • DOI: 10.5812/jjm.36008

To Cite: Mekni M A, Achour W, Ben Hassen A. Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis, Jundishapur J Microbiol. 2017 ; 10(1):e36008. doi: 10.5812/jjm.36008.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Otto M. Staphylococcus colonization of the skin and antimicrobial peptides. Expert Rev Dermatol. 2010; 5(2): 183-95[DOI][PubMed]
  • 2. Mack D, Davies AP, Harris LG, Jeeves R, Pascoe B, Knobloch JKM, et al. Staphylococcus epidermidis in Biomaterial-Associated Infections. Biomaterials Associated Infection: Immunological Aspects and Antimicrobial Strategies. 2013; : 25-56[DOI]
  • 3. Wu H, Moser C, Wang HZ, Hoiby N, Song ZJ. Strategies for combating bacterial biofilm infections. Int J Oral Sci. 2015; 7(1): 1-7[DOI][PubMed]
  • 4. Otto M. Staphylococcus epidermidis--the 'accidental' pathogen. Nat Rev Microbiol. 2009; 7(8): 555-67[DOI][PubMed]
  • 5. Buttner H, Mack D, Rohde H. Structural basis of Staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions. Front Cell Infect Microbiol. 2015; 5: 14[DOI][PubMed]
  • 6. Stevens NT, Greene CM, O'Gara JP, Humphreys H. Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis. J Med Microbiol. 2009; 58: 855-62[DOI][PubMed]
  • 7. Xue T, Ni J, Shang F, Chen X, Zhang M. Autoinducer-2 increases biofilm formation via an ica- and bhp-dependent manner in Staphylococcus epidermidis RP62A. Microbes Infect. 2015; 17(5): 345-52[DOI][PubMed]
  • 8. Arciola CR, Campoccia D, Ravaioli S, Montanaro L. Polysaccharide intercellular adhesin in biofilm: structural and regulatory aspects. Front Cell Infect Microbiol. 2015; 5: 7[DOI][PubMed]
  • 9. Meireles A, Borges A, Giaouris E, Simoes M. The current knowledge on the application of anti-biofilm enzymes in the food industry. Food Res Int. 2016; 86: 140-6[DOI]
  • 10. Guldimann C, Boor KJ, Wiedmann M, Guariglia-Oropeza V. Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria. Appl Environ Microbiol. 2016; 82(15): 4456-69[DOI][PubMed]
  • 11. Sadykov MR, Hartmann T, Mattes TA, Hiatt M, Jann NJ, Zhu Y, et al. CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis. Microbiology. 2011; 157: 3458-68[DOI][PubMed]
  • 12. Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8): 891-9[DOI][PubMed]
  • 13. Mekni MA, Bouchami O, Achour W, Ben Hassen A. Strong biofilm production but not adhesion virulence factors can discriminate between invasive and commensal Staphylococcus epidermidis strains. APMIS. 2012; 120(8): 605-11[DOI][PubMed]
  • 14. Saffari F, Widerstrom M, Gurram BK, Edebro H, Hojabri Z, Monsen T. Molecular and Phenotypic Characterization of Multidrug-Resistant Clones of Staphylococcus epidermidis in Iranian Hospitals: Clonal Relatedness to Healthcare-Associated Methicillin-Resistant Isolates in Northern Europe. Microb Drug Resist. 2016; 22(7): 570-7[DOI][PubMed]
  • 15. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982; 37(1): 318-26[PubMed]
  • 16. Arciola CR, Campoccia D, Gamberini S, Rizzi S, Donati ME, Baldassarri L, et al. Search for the insertion element IS256 within the ica locus of Staphylococcus epidermidis clinical isolates collected from biomaterial-associated infections. Biomaterials. 2004; 25(18): 4117-25[DOI][PubMed]
  • 17. Mack D, Riedewald J, Rohde H, Magnus T, Feucht HH, Elsner HA, et al. Essential functional role of the polysaccharide intercellular adhesin of Staphylococcus epidermidis in hemagglutination. Infect Immun. 1999; 67(2): 1004-8[PubMed]
  • 18. Qin Z, Yang X, Yang L, Jiang J, Ou Y, Molin S, et al. Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates. J Med Microbiol. 2007; 56: 83-93[DOI][PubMed]
  • 19. Ylioinas J, Hadid A, Hong X, Pietikäinen M. Age Estimation Using Local Binary Pattern Kernel Density Estimate . Image Analysis and Processing - ICIAP 2013: 17th International Conference, Naples, Italy. 2013; 8156: 141-50[DOI]
  • 20. Kobayashi H, Oethinger M, Tuohy MJ, Procop GW, Bauer TW. Improved detection of biofilm-formative bacteria by vortexing and sonication: a pilot study. Clin Orthop Relat Res. 2009; 467(5): 1360-4[DOI][PubMed]
  • 21. Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2^(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2013; 3(3): 71-85[PubMed]
  • 22. Begovic J, Jovcic B, Papic-Obradovic M, Veljovic K, Lukic J, Kojic M, et al. Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. Microbiol Res. 2013; 168(2): 77-83[DOI][PubMed]
  • 23. Botelho AM, Nunes Z, Asensi MD, Gomes MZ, Fracalanzza SE, Figueiredo AM. Characterization of coagulase-negative staphylococci isolated from hospital indoor air and a comparative analysis between airborne and inpatient isolates of Staphylococcus epidermidis. J Med Microbiol. 2012; 61: 1136-45[DOI][PubMed]
  • 24. Mateo M, Maestre JR, Aguilar L, Gimenez MJ, Granizo JJ, Prieto J. Strong slime production is a marker of clinical significance in Staphylococcus epidermidis isolated from intravascular catheters. Eur J Clin Microbiol Infect Dis. 2008; 27(4): 311-4[DOI][PubMed]
  • 25. Widerstrom M, Carroll KC. Significance of Staphylococcus epidermidis in Health Care-Associated Infections, from Contaminant to Clinically Relevant Pathogen: This Is a Wake-Up Call! J Clin Microbiol. 2016; 54(7): 1679-81[DOI]
  • 26. Schaeffer CR, Woods KM, Longo GM, Kiedrowski MR, Paharik AE, Buttner H, et al. Accumulation-associated protein enhances Staphylococcus epidermidis biofilm formation under dynamic conditions and is required for infection in a rat catheter model. Infect Immun. 2015; 83(1): 214-26[DOI][PubMed]
  • 27. Dice B, Stoodley P, Buchinsky F, Metha N, Ehrlich GD, Hu FZ. Biofilm formation by ica-positive and ica-negative strains of Staphylococcus epidermidis in vitro. Biofouling. 2009; 25(4): 367-75[DOI][PubMed]
  • 28. Harris LG, Murray S, Pascoe B, Bray J, Meric G, Magerios L, et al. Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples. PLoS One. 2016; 11(3)[DOI][PubMed]
  • 29. Rohde H, Kalitzky M, Kroger N, Scherpe S, Horstkotte MA, Knobloch JK, et al. Detection of virulence-associated genes not useful for discriminating between invasive and commensal Staphylococcus epidermidis strains from a bone marrow transplant unit. J Clin Microbiol. 2004; 42(12): 5614-9[DOI][PubMed]
  • 30. Chessa D, Ganau G, Spiga L, Bulla A, Mazzarello V, Campus GV, et al. Staphylococcus aureus and Staphylococcus epidermidis Virulence Strains as Causative Agents of Persistent Infections in Breast Implants. PLoS One. 2016; 11(1)[DOI][PubMed]
  • 31. Arciola CR, Campoccia D, Speziale P, Montanaro L, Costerton JW. Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials. 2012; 33(26): 5967-82[DOI][PubMed]
  • 32. Hennig S, Ziebuhr W. Characterization of the transposase encoded by IS256, the prototype of a major family of bacterial insertion sequence elements. J Bacteriol. 2010; 192(16): 4153-63[DOI][PubMed]
  • 33. Bronesky D, Wu Z, Marzi S, Walter P, Geissmann T, Moreau K, et al. Staphylococcus aureus RNAIII and Its Regulon Link Quorum Sensing, Stress Responses, Metabolic Adaptation, and Regulation of Virulence Gene Expression. Annu Rev Microbiol. 2016; 70: 299-316[DOI][PubMed]
  • 34. Schommer NN, Christner M, Hentschke M, Ruckdeschel K, Aepfelbacher M, Rohde H. Staphylococcus epidermidis uses distinct mechanisms of biofilm formation to interfere with phagocytosis and activation of mouse macrophage-like cells 774A.1. Infect Immun. 2011; 79(6): 2267-76[DOI][PubMed]
  • 35. Dobinsky S, Kiel K, Rohde H, Bartscht K, Knobloch JK, Horstkotte MA, et al. Glucose-related dissociation between icaADBC transcription and biofilm expression by Staphylococcus epidermidis: evidence for an additional factor required for polysaccharide intercellular adhesin synthesis. J Bacteriol. 2003; 185(9): 2879-86[PubMed]
  • 36. Al Laham N, Rohde H, Sander G, Fischer A, Hussain M, Heilmann C, et al. Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype. J Bacteriol. 2007; 189(12): 4494-501[DOI][PubMed]
  • 37. Sousa C, Teixeira P, Oliveira R. The role of extracellular polymers on Staphylococcus epidermidis biofilm biomass and metabolic activity. J Basic Microbiol. 2009; 49(4): 363-70[DOI][PubMed]
  • 38. Dai L, Yang L, Parsons C, Findlay VJ, Molin S, Qin Z. Staphylococcus epidermidis recovered from indwelling catheters exhibit enhanced biofilm dispersal and "self-renewal" through downregulation of agr. BMC Microbiol. 2012; 12: 102[DOI][PubMed]
  • 39. Ziebuhr W, Krimmer V, Rachid S, Lossner I, Gotz F, Hacker J. A novel mechanism of phase variation of virulence in Staphylococcus epidermidis: evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256. Mol Microbiol. 1999; 32(2): 345-56[PubMed]
  • 40. de Araujo GL, Coelho LR, de Carvalho CB, Maciel RM, Coronado AZ, Rozenbaum R, et al. Commensal isolates of methicillin-resistant Staphylococcus epidermidis are also well equipped to produce biofilm on polystyrene surfaces. J Antimicrob Chemother. 2006; 57(5): 855-64[DOI][PubMed]
  • 41. Nilsson M, Frykberg L, Flock JI, Pei L, Lindberg M, Guss B. A fibrinogen-binding protein of Staphylococcus epidermidis. Infect Immun. 1998; 66(6): 2666-73[PubMed]
  • 42. Williams RJ, Henderson B, Sharp LJ, Nair SP. Identification of a fibronectin-binding protein from Staphylococcus epidermidis. Infect Immun. 2002; 70(12): 6805-10[PubMed]
  • 43. Gu J, Li H, Li M, Vuong C, Otto M, Wen Y, et al. Bacterial insertion sequence IS256 as a potential molecular marker to discriminate invasive strains from commensal strains of Staphylococcus epidermidis. J Hosp Infect. 2005; 61(4): 342-8[DOI][PubMed]
  • 44. Li M, Wang X, Gao Q, Lu Y. Molecular characterization of Staphylococcus epidermidis strains isolated from a teaching hospital in Shanghai, China. J Med Microbiol. 2009; 58: 456-61[DOI][PubMed]
  • 45. Pintens V, Massonet C, Merckx R, Vandecasteele S, Peetermans WE, Knobloch JK, et al. The role of sigmaB in persistence of Staphylococcus epidermidis foreign body infection. Microbiology. 2008; 154: 2827-36[DOI][PubMed]
  • 46. Frebourg NB, Lefebvre S, Baert S, Lemeland JF. PCR-Based assay for discrimination between invasive and contaminating Staphylococcus epidermidis strains. J Clin Microbiol. 2000; 38(2): 877-80[PubMed]
  • 47. Vandecasteele SJ, Peetermans WE, Merckx R, Van Eldere J. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions. J Bacteriol. 2001; 183(24): 7094-101[DOI][PubMed]
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