Jundishapur Journal of Microbiology

Published by: Kowsar

Frequency of Aminoglycoside-Resistance Genes in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitalized Patients

Seyed Mohsen Mahdiyoun 1 , Hossein Kazemian 2 , Mohammad Ahanjan 3 , Hamidreza Houri 4 and Mehdi Goudarzi 4 , *
Authors Information
1 Department of Microbiology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran
2 Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Microbiology, Molecular and Cellular Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran
4 Department of Medical Microbiology School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 01, 2016, 9 (8); e35052
  • Published Online: July 26, 2016
  • Article Type: Research Article
  • Received: November 27, 2015
  • Revised: June 20, 2016
  • Accepted: June 28, 2016
  • DOI: 10.5812/jjm.35052

To Cite: Mahdiyoun S M, Kazemian H, Ahanjan M, Houri H, Goudarzi M. Frequency of Aminoglycoside-Resistance Genes in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitalized Patients, Jundishapur J Microbiol. 2016 ; 9(8):e35052. doi: 10.5812/jjm.35052.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Al-Khulaifi Manal M, Amin Aref Nagwa M, Al Salamah AA. Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus. Saudi J Biol Sci. 2009; 16(1): 37-49[DOI][PubMed]
  • 2. Hu Y, Liu A, Vaudrey J, Vaiciunaite B, Moigboi C, McTavish SM, et al. Combinations of beta-lactam or aminoglycoside antibiotics with plectasin are synergistic against methicillin-sensitive and methicillin-resistant Staphylococcus aureus. PLoS One. 2015; 10(2)[DOI][PubMed]
  • 3. Pillai MM, Latha R, Sarkar G. Detection of methicillin resistance in Staphylococcus aureus by polymerase chain reaction and conventional methods: a comparative study. J Lab Physicians. 2012; 4(2): 83-8[DOI][PubMed]
  • 4. Nowrouzian FL, Karami N, Welinder-Olsson C, Ahren C. Virulence gene typing of methicillin-resistant Staphylococcus aureus as a complement in epidemiological typing. J Microbiol Methods. 2013; 93(3): 173-6[DOI][PubMed]
  • 5. Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, et al. Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J Dairy Sci. 2007; 90(3): 1176-85[DOI][PubMed]
  • 6. Tkadlec J, Varekova E, Pantucek R, Doskar J, Ruzickova V, Botka T, et al. Characterization of Staphylococcus aureus Strains Isolated from Czech Cystic Fibrosis Patients: High Rate of Ribosomal Mutation Conferring Resistance to MLS(B) Antibiotics as a Result of Long-Term and Low-Dose Azithromycin Treatment. Microb Drug Resist. 2015; 21(4): 416-23[DOI][PubMed]
  • 7. Gade ND, Qazi MS. Recent trend of aminoglycoside resistance among Staphylococcus aureus isolates in tertiary care hospital. J Microbiol Antimicrob. 2014; 6(6): 94-6[DOI]
  • 8. Schmitz FJ, Fluit AC, Gondolf M, Beyrau R, Lindenlauf E, Verhoef J, et al. The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals. J Antimicrob Chemother. 1999; 43(2): 253-9[PubMed]
  • 9. Edson RS, Terrell CL. The aminoglycosides. Mayo Clin Proc. 1999; 74(5): 519-28[DOI][PubMed]
  • 10. Alli O, Ogbolu D, Bamigboye K, Animasaun A, Oluremi A. Distribution of genes encoding aminoglycoside modifying enzymes amongst methicillin resistant and methicillin sensitive Staphylococcus aureus isolates from Nigerian hospitals. Afr J Microbiol Res. 2015; 9(5): 318-25[DOI]
  • 11. Fard-Mousavi N, Mosayebi G, Amouzandeh-Nobaveh A, Japouni-Nejad A, Ghaznavi-Rad E. The Dynamic of Staphylococcus aureus Nasal Carriage in Central Iran. Jundishapur J Microbiol. 2015; 8(7): 20760[DOI][PubMed]
  • 12. Ebrahim-Saraie HS, Motamedifar M, Sarvari J, Hoseini Alfatemi SM. Emergence of SCCmec Type I Obtained From Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran. Jundishapur J Microbiol. 2015; 8(6): 16998[DOI][PubMed]
  • 13. Taherikalani M, Mohammadzad MR, Soroush S, Maleki MH, Azizi-Jalilian F, Pakzad I, et al. Determining the prevalence of SCCmec polymorphism, virulence and antibiotic resistance genes among methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from selected hospitals in west of Iran. J Chemother. 2016; 28(2): 104-9[DOI][PubMed]
  • 14. Kali A, Stephen S, Umadevi S. Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus. Biomed J. 2014; 37(6): 411-4[DOI][PubMed]
  • 15. Clinical and laboratory standards institute (clsi) m100-s24 performance standards for antimicrobial susceptibility testing 2014;
  • 16. Dibah S, Arzanlou M, Jannati E, Shapouri R. Prevalence and antimicrobial resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens in Ardabil, Iran. Iran J Microbiol. 2014; 6(3): 163-8[PubMed]
  • 17. Martineau F, Picard FJ, Roy PH, Ouellette M, Bergeron MG. Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. J Clin Microbiol. 1998; 36(3): 618-23[PubMed]
  • 18. Vanhoof R, Godard C, Content J, Nyssen HJ, Hannecart-Pokorni E. Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. Belgian Study Group of Hospital Infections (GDEPIH/GOSPIZ). J Med Microbiol. 1994; 41(4): 282-90[DOI][PubMed]
  • 19. Choi SM, Kim SH, Kim HJ, Lee DG, Choi JH, Yoo JH, et al. Multiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species. J Korean Med Sci. 2003; 18(5): 631-6[DOI][PubMed]
  • 20. Havaei SA, Azimian A, Fazeli H, Naderi M, Ghazvini K, Samiee SM, et al. Isolation of Asian endemic and livestock associated clones of methicillin resistant Staphylococcus aureus from ocular samples in Northeastern Iran. Iran J Microbiol. 2013; 5(3): 227-32[PubMed]
  • 21. Hasani A, Sheikhalizadeh V, Hasani A, Naghili B, Valizadeh V, Nikoonijad AR. Methicillin resistant and susceptible Staphylococcus aureus: Appraising therapeutic approaches in the Northwest of Iran. Iran J Microbiol. 2013; 5(1): 56-62[PubMed]
  • 22. Rapp RP. Overview of resistant gram-positive pathogens in the surgical patient. Surg Infect (Larchmt). 2000; 1(1): 39-47[DOI][PubMed]
  • 23. Askari E, Soleymani F, Arianpoor A, Tabatabai SM, Amini A, Naderinasab M. Epidemiology of mecA-Methicillin Resistant Staphylococcus aureus (MRSA) in Iran: A Systematic Review and Meta-analysis. Iran J Basic Med Sci. 2012; 15(5): 1010-9[PubMed]
  • 24. Emaneini M, Bigverdi R, Kalantar D, Soroush S, Jabalameli F, Noorazar Khoshgnab B, et al. Distribution of genes encoding tetracycline resistance and aminoglycoside modifying enzymes in Staphylococcus aureus strains isolated from a burn center. Ann Burns Fire Disasters. 2013; 26(2): 76-80[PubMed]
  • 25. Zembower TR, Noskin GA, Postelnick MJ, Nguyen C, Peterson LR. The utility of aminoglycosides in an era of emerging drug resistance. Int J Antimicrob Agents. 1998; 10(2): 95-105[PubMed]
  • 26. Mulazimoglu L, Drenning SD, Muder RR. Vancomycin-gentamicin synergism revisited: effect of gentamicin susceptibility of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1996; 40(6): 1534-5[PubMed]
  • 27. Shaw KJ, Rather PN, Hare RS, Miller GH. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev. 1993; 57(1): 138-63[PubMed]
  • 28. Ounissi H, Derlot E, Carlier C, Courvalin P. Gene homogeneity for aminoglycoside-modifying enzymes in gram-positive cocci. Antimicrob Agents Chemother. 1990; 34(11): 2164-8[PubMed]
  • 29. Udo EE, Dashti AA. Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization. Int J Antimicrob Agents. 2000; 13(4): 273-9[PubMed]
  • 30. Ardic N, Sareyyupoglu B, Ozyurt M, Haznedaroglu T, Ilga U. Investigation of aminoglycoside modifying enzyme genes in methicillin-resistant staphylococci. Microbiol Res. 2006; 161(1): 49-54[DOI][PubMed]
  • 31. Ida T, Okamoto R, Shimauchi C, Okubo T, Kuga A, Inoue M. Identification of aminoglycoside-modifying enzymes by susceptibility testing: epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J Clin Microbiol. 2001; 39(9): 3115-21[PubMed]
  • 32. Yadegar A, Satri M, Mozafari N. Pervalence ant (4’)-Ia gene among nosocomial methicillin resistant Staphylococcus aureus by Multiplex-PCR. MJMS. 2010; 1: 59-68
  • 33. Kazemian H, Ghafourian S, Heidari H, Amiri P, Yamchi JK, Shavalipour A, et al. Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa. Rev Soc Bras Med Trop. 2015; 48(4): 432-6[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments