Jundishapur Journal of Microbiology

Published by: Kowsar

Virulence Gene Profile and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) of Enteroinvasive Escherichia coli (EIEC) Isolates From Patients With Diarrhea in Kerman, Iran

Hossein Hosseini Nave 1 , Shahla Mansouri 1 , Majid Taati Moghadam 1 and Mohammad Moradi 1 , *
Authors Information
1 Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, IR Iran
Article information
  • Jundishapur Journal of Microbiology: June 01, 2016, 9 (6); e33529
  • Published Online: May 24, 2016
  • Article Type: Research Article
  • Received: October 4, 2015
  • Revised: March 17, 2016
  • Accepted: April 11, 2016
  • DOI: 10.5812/jjm.33529

To Cite: Hosseini Nave H, Mansouri S, Taati Moghadam M, Moradi M. Virulence Gene Profile and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) of Enteroinvasive Escherichia coli (EIEC) Isolates From Patients With Diarrhea in Kerman, Iran, Jundishapur J Microbiol. 2016 ; 9(6):e33529. doi: 10.5812/jjm.33529.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Sousa CP. East1 toxin and its presence in a changing microbial world. J Venom Anim Toxins Incl Trop Dis. 2003; 9(1): 4-52[DOI]
  • 2. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004; 2(2): 123-40[DOI][PubMed]
  • 3. Abbasi P, Kargar M, Doosti A, Mardaneh J, Dalini SG, Dehyadegari MA. Real time pcr for characterization of enteroinvasive e. Coli (eiec) in children with diarrhea in shiraz. Ann Colorectal Res. 2015; 2(3)[DOI]
  • 4. Martinez MB, Whittan TS, McGraw EA, Rodrigues J, Trabulsi LR. Clonal relationship among invasive and non-invasive strains of enteroinvasive Escherichia coli serogroups. FEMS Microbiol Lett. 1999; 172(2): 145-51[PubMed]
  • 5. Sousa MA, Mendes EN, Collares GB, Peret-Filho LA, Penna FJ, Magalhaes PP. Shigella in Brazilian children with acute diarrhoea: prevalence, antimicrobial resistance and virulence genes. Mem Inst Oswaldo Cruz. 2013; 108(1): 30-5[PubMed]
  • 6. Cruz C, Souza M, Serra P, Santos I, Balieiro A, Pieri FA, et al. Virulence factors associated with pediatric shigellosis in Brazilian Amazon. Biomed Res Int. 2014; 2014[DOI]
  • 7. Broach WH, Egan N, Wing HJ, Payne SM, Murphy ER. VirF-independent regulation of Shigella virB transcription is mediated by the small RNA RyhB. PLoS One. 2012; 7(6)[DOI][PubMed]
  • 8. Boisen N, Ruiz-Perez F, Scheutz F, Krogfelt KA, Nataro JP. Short report: high prevalence of serine protease autotransporter cytotoxins among strains of enteroaggregative Escherichia coli. Am J Trop Med Hyg. 2009; 80(2): 294-301[PubMed]
  • 9. Kawamori F, Hiroi M, Harada T, Ohata K, Sugiyama K, Masuda T, et al. Molecular typing of Japanese Escherichia coli O157 : H7 isolates from clinical specimens by multilocus variable-number tandem repeat analysis and PFGE. J Med Microbiol. 2008; 57: 58-63[DOI][PubMed]
  • 10. Ranjbar R, Memariani M. Multilocus variable-number tandem-repeat analysis for genotyping of Shigella sonnei strains isolated from pediatric patients. Gastroenterol Hepatol Bed Bench. 2015; 8(3): 225-32[PubMed]
  • 11. Memariani M, Peerayeh SN, Salehi TZ, Mostafavi SKS. Occurrence of shv, tem and ctx-m β-lactamase genes among enteropathogenic escherichia coli strains isolated from children with diarrhea. Jundishapur J Microbiol 2015; 8(4)[DOI]
  • 12. Wang YW, Watanabe H, Phung DC, Tung SK, Lee YS, Terajima J, et al. Multilocus variable-number tandem repeat analysis for molecular typing and phylogenetic analysis of Shigella flexneri. BMC Microbiol. 2009; 9: 278[DOI][PubMed]
  • 13. Liang SY, Watanabe H, Terajima J, Li CC, Liao JC, Tung SK, et al. Multilocus variable-number tandem-repeat analysis for molecular typing of Shigella sonnei. J Clin Microbiol. 2007; 45(11): 3574-80[DOI][PubMed]
  • 14. Gorge O, Lopez S, Hilaire V, Lisanti O, Ramisse V, Vergnaud G. Selection and validation of a multilocus variable-number tandem-repeat analysis panel for typing Shigella spp. J Clin Microbiol. 2008; 46(3): 1026-36[DOI][PubMed]
  • 15. Chiou CS, Watanabe H, Wang YW, Wang WL, Terajima J, Thong KL, et al. Utility of multilocus variable-number tandem-repeat analysis as a molecular tool for phylogenetic analysis of Shigella sonnei. J Clin Microbiol. 2009; 47(4): 1149-54[DOI][PubMed]
  • 16. Bonkoungou IJ, Lienemann T, Martikainen O, Dembele R, Sanou I, Traore AS, et al. Diarrhoeagenic Escherichia coli detected by 16-plex PCR in children with and without diarrhoea in Burkina Faso. Clin Microbiol Infect. 2012; 18(9): 901-6[DOI][PubMed]
  • 17. van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect. 2007; 13 Suppl 3: 1-46[DOI][PubMed]
  • 18. Katouli M, Jaafari A, Farhoudi-Moghaddam AA, Ketabi GR. Aetiological studies of diarrhoeal diseases in infants and young children in Iran. J Trop Med Hyg. 1990; 93(1): 22-7[PubMed]
  • 19. Rappelli P, Folgosa E, Solinas ML, Dacosta JL, Pisanu C, Sidat M, et al. Pathogenic enteric Escherichia coli in children with and without diarrhea in Maputo, Mozambique. FEMS Immunol Med Microbiol. 2005; 43(1): 67-72[DOI][PubMed]
  • 20. Regua-Mangia AH, Gomes TA, Vieira MA, Andrade JR, Irino K, Teixeira LM. Frequency and characteristics of diarrhoeagenic Escherichia coli strains isolated from children with and without diarrhoea in Rio de Janeiro, Brazil. J Infect. 2004; 48(2): 161-7[PubMed]
  • 21. Teng LJ, Hsueh PR, Liaw SJ, Ho SW, Tsai JC. Genetic detection of diarrheagenic Escherichia coli isolated from children with sporadic diarrhea. J Microbiol Immunol Infect. 2004; 37(6): 327-34[PubMed]
  • 22. Vieira N, Bates SJ, Solberg OD, Ponce K, Howsmon R, Cevallos W, et al. High prevalence of enteroinvasive Escherichia coli isolated in a remote region of northern coastal Ecuador. Am J Trop Med Hyg. 2007; 76(3): 528-33[PubMed]
  • 23. Kain KC, Barteluk RL, Kelly MT, He X, de Hua G, Ge YA, et al. Etiology of childhood diarrhea in Beijing, China. J Clin Microbiol. 1991; 29(1): 90-5[PubMed]
  • 24. Thong KL, Hoe SL, Puthucheary SD, Yasin R. Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay. BMC Infect Dis. 2005; 5: 8[DOI][PubMed]
  • 25. Kingombe CI, Cerqueira-Campos ML, Farber JM. Molecular strategies for the detection, identification, and differentiation between enteroinvasive Escherichia coli and Shigella spp. J Food Prot. 2005; 68(2): 239-45[PubMed]
  • 26. Su WL, Chen C, Wang ZQ, Li J, He X, Sun ZN, et al. [Virulence genes and pathogenicity of Shigella flexneri Xv isolated in Beijing]. Zhonghua Liu Xing Bing Xue Za Zhi. 2013; 34(1): 57-60[PubMed]
  • 27. Vargas M, Gascon J, Jimenez De Anta MT, Vila J. Prevalence of Shigella enterotoxins 1 and 2 among Shigella strains isolated from patients with traveler's diarrhea. J Clin Microbiol. 1999; 37(11): 3608-11[PubMed]
  • 28. Muller D, Greune L, Heusipp G, Karch H, Fruth A, Tschape H, et al. Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR. Appl Environ Microbiol. 2007; 73(10): 3380-90[DOI][PubMed]
  • 29. Taddei CR, Moreno ACR, Fernandes Filho A, Montemor LPG, Martinez MB. Prevalence of secreted autotransporter toxin gene among diffusely adheringEscherichia coliisolated from stools of children. FEMS Microbiol Lett. 2003; 227(2): 249-53[DOI]
  • 30. Gibotti A, Tanaka T, Oliveira V, Taddei CR, Martinez MB. Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis. Braz J Microbiol. 2004; 35(1-2): 74-80[DOI]
  • 31. Beutin L, Gleier K, Kontny I, Echeverria P, Scheutz F. Origin and characteristics of enteroinvasive strains of Escherichia coli (EIEC) isolated in Germany. Epidemiol Infect. 1997; 118(3): 199-205[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments