Jundishapur Journal of Microbiology

Published by: Kowsar

Optimized Expression, Purification of Herpes B Virus gD Protein in Escherichia coli, and Production of Its Monoclonal Antibodies

Zian Jin 1 , Tao Sun 1 , Xueshan Xia 1 , Qiujiang Wei 2 , Yuzhu Song 1 , Qinqin Han 1 , Qiang Chen 1 , Juan Hu 1 and Jinyang Zhang 1 , *
Authors Information
1 Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China
2 Kunming Biomed International Company, Kunming, China
Article information
  • Jundishapur Journal of Microbiology: March 01, 2016, 9 (3); e32183
  • Published Online: March 12, 2016
  • Article Type: Research Article
  • Received: August 27, 2015
  • Revised: October 23, 2015
  • Accepted: January 18, 2016
  • DOI: 10.5812/jjm.32183

To Cite: Jin Z, Sun T, Xia X, Wei Q, Song Y, et al. Optimized Expression, Purification of Herpes B Virus gD Protein in Escherichia coli, and Production of Its Monoclonal Antibodies, Jundishapur J Microbiol. 2016 ; 9(3):e32183. doi: 10.5812/jjm.32183.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Perelygina L, Zhu L, Zurkuhlen H, Mills R, Borodovsky M, Hilliard JK. Complete sequence and comparative analysis of the genome of herpes B virus (Cercopithecine herpesvirus 1) from a rhesus monkey. J Virol. 2003; 77(11): 6167-77[PubMed]
  • 2. Jainkittivong A, Langlais RP. Herpes B virus infection. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 4(85): 399-403
  • 3. Elmore D, Eberle R. Monkey B virus (Cercopithecine herpesvirus 1). Comp Med. 2008; 58(1): 11-21[PubMed]
  • 4. Leas BF, Umscheid CA. Neonatal Herpes Simplex Virus Type 1 Infection and Jewish Ritual Circumcision With Oral Suction: A Systematic Review. J Pediatric Infect Dis Soc. 2015; 4(2): 126-31[DOI][PubMed]
  • 5. Eberle R, Hilliard J. The simian herpesviruses. Infect Agents Dis. 1995; 4(2): 55-70[PubMed]
  • 6. Lee CC, Lin LL, Chan WE, Ko TP, Lai JS, Wang AH. Structural basis for the antibody neutralization of herpes simplex virus. Acta Crystallogr D Biol Crystallogr. 2013; 69: 1935-45[DOI][PubMed]
  • 7. Huff JL, Barry PA. B-virus (Cercopithecine herpesvirus 1) infection in humans and macaques: potential for zoonotic disease. Emerg Infect Dis. 2003; 9(2): 246-50[DOI][PubMed]
  • 8. Whitbeck JC, Huang ZY, Cairns TM, Gallagher JR, Lou H, Ponce-de-Leon M, et al. Repertoire of epitopes recognized by serum IgG from humans vaccinated with herpes simplex virus 2 glycoprotein D. J Virol. 2014; 88(14): 7786-95[DOI][PubMed]
  • 9. Zwartouw HT, Boulter EA. Excretion of B virus in monkeys and evidence of genital infection. Lab Anim. 1984; 18(1): 65-70[PubMed]
  • 10. Perelygina L, Patrusheva I, Hombaiah S, Zurkuhlen H, Wildes MJ, Patrushev N, et al. Production of herpes B virus recombinant glycoproteins and evaluation of their diagnostic potential. J Clin Microbiol. 2005; 43(2): 620-8[DOI][PubMed]
  • 11. Blewett EL, Saliki JT, Eberle R. Development of a competitive ELISA for detection of primates infected with monkey B virus (Herpesvirus simiae). J Virol Methods. 1999; 77(1): 59-67[PubMed]
  • 12. Norcott JP, Brown DW. Competitive radioimmunoassay to detect antibodies to herpes B virus and SA8 virus. J Clin Microbiol. 1993; 31(4): 931-5[PubMed]
  • 13. Belabassi Y, Chao CK, Holly R, George KM, Nagy JO, Thompson CM. Preparation and characterization of diethoxy- and monoethoxy phosphylated ('aged') serine haptens and use in the production of monoclonal antibodies. Chem Biol Interact. 2014; 223C: 134-40[DOI][PubMed]
  • 14. Ward JA, Hilliard JK. B virus-specific pathogen-free (SPF) breeding colonies of macaques: issues, surveillance, and results in 1992. Lab Anim Sci. 1994; 44(3): 222-8[PubMed]
  • 15. Eberle R, Black D, Hilliard JK. Relatedness of glycoproteins expressed on the surface of simian herpes-virus virions and infected cells to specific HSV glycoproteins. Arch Virol. 1989; 109(3-4): 233-52[PubMed]
  • 16. Hilliard JK, Black D, Eberle R. Simian alphaherpesviruses and their relation to the human herpes simplex viruses. Arch Virol. 1989; 109(1-2): 83-102[PubMed]
  • 17. Atanasiu D, Cairns TM, Whitbeck JC, Saw WT, Rao S, Eisenberg RJ, et al. Regulation of herpes simplex virus gB-induced cell-cell fusion by mutant forms of gH/gL in the absence of gD and cellular receptors. MBio. 2013; 4(2)[DOI][PubMed]
  • 18. Bernstein DI, Bryson YJ, Lovett MA. Antibody response to type-common and type-unique epitopes of herpes simplex virus polypeptides. J Med Virol. 1985; 15(3): 251-63[PubMed]
  • 19. Eberle R, Mou SW, Zaia JA. The immune response to herpes simplex virus: comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes simplex virus type 1 infections. J Med Virol. 1985; 16(2): 147-62[PubMed]
  • 20. Perelygina L, Zurkuhlen H, Patrusheva I, Hilliard JK. Identification of a herpes B virus-specific glycoprotein d immunodominant epitope recognized by natural and foreign hosts. J Infect Dis. 2002; 186(4): 453-61[DOI][PubMed]
  • 21. Bernstein DI, Aoki FY, Tyring SK, Stanberry LR, St-Pierre C, Shafran SD, et al. Safety and immunogenicity of glycoprotein D-adjuvant genital herpes vaccine. Clin Infect Dis. 2005; 40(9): 1271-81[DOI][PubMed]
  • 22. Fan Q, Amen M, Harden M, Severini A, Griffiths A, Longnecker R. Herpes B virus utilizes human nectin-1 but not HVEM or PILRalpha for cell-cell fusion and virus entry. J Virol. 2012; 86(8): 4468-76[DOI][PubMed]
  • 23. Stump JD, Sticht H. Mutations in herpes simplex virus gD protein affect receptor binding by different molecular mechanisms. J Mol Model. 2014; 20(4): 2192[DOI][PubMed]
  • 24. Zhang J, Han Q, Song Y, Chen Q, Xia X. Analysis of Subcellular Prefoldin 1 Redistribution During Rabies Virus Infection. Jundishapur J Microbiol. 2015; 8(7)[DOI][PubMed]
  • 25. Berry JD, Jones S, Drebot MA, Andonov A, Sabara M, Yuan XY, et al. Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. J Virol Methods. 2004; 120(1): 87-96[DOI][PubMed]
  • 26. Zhang J, Sun T, Yang G, Song Y, Han Q, Xia X. Prediction of B Cell Epitopes and Major Characteristics in Protein gG of M onkey Herpes B Virus. J Kunming Uni Sci Tech. 2014; 2(39): 79-85
  • 27. Behrouzi A, Bouzari S, Siadat SD, Jafari A, Irani S. Molecular Cloning, Expression and Purification of Truncated hpd Fragment of Haemophilus influenzae in Escherichia coli. Jundishapur J Microbiol. 2015; 8(8)[DOI][PubMed]
  • 28. Costa NL, Carlson HK, Coates JD, Louro RO, Paquete CM. Heterologous expression and purification of a multiheme cytochrome from a Gram-positive bacterium capable of performing extracellular respiration. Protein Expr Purif. 2015; 111: 48-52[DOI][PubMed]
  • 29. Ben Hamadou-Charfi D, Sauer AJ, Abdelkafi-Mesrati L, Jaoua S, Stephan D. Production of polyclonal and monoclonal antibodies against the Bacillus thuringiensis vegetative insecticidal protein Vip3Aa16. Appl Biochem Biotechnol. 2015; 175(5): 2357-65[DOI][PubMed]
  • 30. Kusano N, Iwanami T, Narahara K, Tanaka M. Production of monoclonal antibodies specific for the recombinant viral coat protein of Apple stem grooving virus-citrus isolate and their application for a simple, rapid diagnosis by an immunochromatographic assay. J Virol Methods. 2014; 195: 86-91[DOI][PubMed]
  • 31. Brady CP, Shimp RL, Miles AP, Whitmore M, Stowers AW. High-level production and purification of P30P2MSP1(19), an important vaccine antigen for malaria, expressed in the methylotropic yeast Pichia pastoris. Protein Expr Purif. 2001; 23(3): 468-75[DOI][PubMed]
  • 32. Huang Y, Yao S. Expression, purification of herpes simplex virus type 1 US11 protein, and production of US11 polyclonal antibody. Virol J. 2011; 8: 490[DOI][PubMed]
  • 33. Zhu Q, Guo D, Feng L, Sun D. Expression and Purification of the scFv from hybridoma cells secreting a monoclonal antibody against S PROTEIN of PEDV. Monoclon Antib Immunodiagn Immunother. 2013; 32(1): 41-6[DOI][PubMed]
  • 34. Shi M, Zhou Y, Cao L, Ding C, Ji Y, Jiang Q, et al. Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application. Braz J Microbiol. 2013; 44(4): 1215-22[DOI][PubMed]
  • 35. Zuo Y, Yang J, He J, Zhao Y, He Y. An uncoordinated-5 homolog B receptor monoclonal antibody regulates A375 melanoma cell migration. Monoclon Antib Immunodiagn Immunother. 2014; 33(4): 280-6[DOI][PubMed]
  • 36. Shang H, Xie Y, Zhou X, Qian Y, Wu J. Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus. Virol J. 2011; 8: 228[DOI][PubMed]
  • 37. Vasireddi M, Hilliard J. Herpes B virus, macacine herpesvirus 1, breaks simplex virus tradition via major histocompatibility complex class I expression in cells from human and macaque hosts. J Virol. 2012; 86(23): 12503-11[DOI][PubMed]
  • 38. Fujima A, Ochiai Y, Saito A, Omori Y, Noda A, Kazuyama Y, et al. Discrimination of antibody to herpes B virus from antibody to herpes simplex virus types 1 and 2 in human and macaque sera. J Clin Microbiol. 2008; 46(1): 56-61[DOI][PubMed]
  • 39. Tanabayashi K, Mukai R, Yamada A. Detection of B virus antibody in monkey sera using glycoprotein D expressed in mammalian cells. J Clin Microbiol. 2001; 39(9): 3025-30[PubMed]
  • 40. Gopinath SC, Hayashi K, Kumar PK. Aptamer that binds to the gD protein of herpes simplex virus 1 and efficiently inhibits viral entry. J Virol. 2012; 86(12): 6732-44[DOI][PubMed]
  • 41. Zhang J, Ruan X, Zan J, Zheng X, Yan Y, Liao M, et al. Efficient generation of monoclonal antibodies against major structural proteins of rabies virus with suckling mouse brain antigen. Monoclon Antib Immunodiagn Immunother. 2014; 33(2): 94-100[DOI][PubMed]
  • 42. Gaughan CL. The present state of the art in expression, production and characterization of monoclonal antibodies. Mol Divers. 2016; 20(1): 255-70[DOI][PubMed]
  • 43. Jones JD. Leishmania tarentolae: an alternative approach to the production of monoclonal antibodies to treat emerging viral infections. Infect Dis Poverty. 2015; 4: 8[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments