Jundishapur Journal of Microbiology

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Evaluation of Biofilm Formation Among Klebsiella pneumoniae Isolates and Molecular Characterization by ERIC-PCR

Kimia Seifi 1 , Hossein Kazemian 2 , Hamid Heidari 3 , Fereshteh Rezagholizadeh 2 , Yasaman Saee 4 , Fariba Shirvani 1 and Hamidreza Houri 5 , *
Authors Information
1 Pediatric Infections Research Center, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
2 Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran
4 Department of Microbiology, Islamic Azad University, Pharmaceutical Branch, Tehran, IR Iran
5 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 01, 2016, 9 (1); e30682
  • Published Online: January 2, 2016
  • Article Type: Research Article
  • Received: June 16, 2015
  • Revised: September 16, 2015
  • Accepted: September 29, 2015
  • DOI: 10.5812/jjm.30682

To Cite: Seifi K, Kazemian H, Heidari H, Rezagholizadeh F, Saee Y, et al. Evaluation of Biofilm Formation Among Klebsiella pneumoniae Isolates and Molecular Characterization by ERIC-PCR, Jundishapur J Microbiol. 2016 ; 9(1):e30682. doi: 10.5812/jjm.30682.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Deretic V, Schurr MJ, Boucher JC, Martin DW. Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J Bacteriol. 1994; 176(10): 2773-80[PubMed]
  • 2. Rayner MG, Zhang Y, Gorry MC, Chen Y, Post JC, Ehrlich GD. Evidence of bacterial metabolic activity in culture-negative otitis media with effusion. JAMA. 1998; 279(4): 296-9[PubMed]
  • 3. Potera C. Forging a link between biofilms and disease. Science. 1999; 283(5409): 1837[PubMed]
  • 4. Blaser J, Vergeres P, Widmer AF, Zimmerli W. In vivo verification of in vitro model of antibiotic treatment of device-related infection. Antimicrob Agents Chemother. 1995; 39(5): 1134-9[PubMed]
  • 5. Costerton JW, Montanaro L, Arciola CR. Biofilm in implant infections: its production and regulation. Int J Artif Organs. 2005; 28(11): 1062-8[PubMed]
  • 6. Ward KH, Olson ME, Lam K, Costerton JW. Mechanism of persistent infection associated with peritoneal implants. J Med Microbiol. 1992; 36(6): 406-13[DOI][PubMed]
  • 7. Edmiston Jr CE, McBain AJ, Roberts C, Leaper D. Clinical and microbiological aspects of biofilm-associated surgical site infections. Adv Exp Med Biol. 2015; 830: 47-67[DOI][PubMed]
  • 8. Ahmed S, Darouiche RO. Anti-biofilm agents in control of device-related infections. Adv Exp Med Biol. 2015; 831: 137-46[DOI][PubMed]
  • 9. Anderl JN, Franklin MJ, Stewart PS. Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother. 2000; 44(7): 1818-24[PubMed]
  • 10. Ghafourian S, Mohebi R, Rezaei M, Raftari M, Sekawi Z, Kazemian H, et al. Comparative analysis of biofilm development among MRSA and MSSA strains. Roum Arch Microbiol Immunol. 2012; 71(4): 175-82[PubMed]
  • 11. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418): 1318-22[PubMed]
  • 12. Tai AY, Stuart RL, Sidjabat HE, Lemoh CN, Rogers BA, Graham M, et al. Local acquisition and nosocomial transmission of Klebsiella pneumoniae harbouring the blaNDM-1 gene in Australia. Med J Aust. 2015; 202(5): 270-2[PubMed]
  • 13. Ahmad S, Abulhamd A. Phenotypic and molecular characterization of nosocomial K. pneumoniae isolates by ribotyping. Adv Med Sci. 2015; 60(1): 69-75[DOI][PubMed]
  • 14. Li B, Zhao Y, Liu C, Chen Z, Zhou D. Molecular pathogenesis of Klebsiella pneumoniae. Future Microbiol. 2014; 9(9): 1071-81[DOI][PubMed]
  • 15. Nitzan O, Elias M, Chazan B, Saliba W. Urinary tract infections in patients with type 2 diabetes mellitus: review of prevalence, diagnosis, and management. Diabetes Metab Syndr Obes. 2015; 8: 129-36[DOI][PubMed]
  • 16. Bennett CJ, Young MN, Darrington H. Differences in urinary tract infections in male and female spinal cord injury patients on intermittent catheterization. Paraplegia. 1995; 33(2): 69-72[DOI][PubMed]
  • 17. Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998; 11(4): 589-603[PubMed]
  • 18. Murphy CN, Clegg S. Klebsiella pneumoniae and type 3 fimbriae: nosocomial infection, regulation and biofilm formation. Future Microbiol. 2012; 7(8): 991-1002[DOI][PubMed]
  • 19. LeChevallier MW, Cawthon CD, Lee RG. Factors promoting survival of bacteria in chlorinated water supplies. Appl Environ Microbiol. 1988; 54(3): 649-54[PubMed]
  • 20. Vuotto C, Longo F, Balice MP, Donelli G, Varaldo PE. Antibiotic Resistance Related to Biofilm Formation in Klebsiella pneumoniae. Pathogens. 2014; 3(3): 743-58[DOI][PubMed]
  • 21. Tille P. Bailey & Scott's diagnostic microbiology. 2013;
  • 22. Mowat E, Butcher J, Lang S, Williams C, Ramage G. Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J Med Microbiol. 2007; 56: 1205-12[DOI][PubMed]
  • 23. Duan H, Chai T, Liu J, Zhang X, Qi C, Gao J, et al. Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR. Environ Res. 2009; 109(5): 511-7[DOI][PubMed]
  • 24. Cartelle M, del Mar Tomas M, Pertega S, Beceiro A, Dominguez MA, Velasco D, et al. Risk factors for colonization and infection in a hospital outbreak caused by a strain of Klebsiella pneumoniae with reduced susceptibility to expanded-spectrum cephalosporins. J Clin Microbiol. 2004; 42(9): 4242-9[DOI][PubMed]
  • 25. Bjarnsholt T. The role of bacterial biofilms in chronic infections. APMIS Suppl. 2013; (136): 1-51[DOI][PubMed]
  • 26. Borges A, Saavedra MJ, Simoes M. Insights on antimicrobial resistance, biofilms and the use of phytochemicals as new antimicrobial agents. Curr Med Chem. 2015; 22(21): 2590-614[PubMed]
  • 27. Yang D, Zhang Z. Biofilm-forming Klebsiella pneumoniae strains have greater likelihood of producing extended-spectrum beta-lactamases. J Hosp Infect. 2008; 68(4): 369-71[DOI][PubMed]
  • 28. Saeed EA, Bnyan IA, Saadi M. Quorum sensing and Biofilm formation by Bacterial Isolates from Hemodialysis Patients. Res Pharm . 2013; 3(2): 33-40
  • 29. Saxena S, Banerjee G, Garg R, Singh M. Comparative Study of Biofilm Formation in Pseudomonas aeruginosa Isolates from Patients of Lower Respiratory Tract Infection. J Clin Diagn Res. 2014; 8(5)-11[DOI][PubMed]
  • 30. Wu H, Moser C, Wang HZ, Hoiby N, Song ZJ. Strategies for combating bacterial biofilm infections. Int J Oral Sci. 2015; 7(1): 1-7[DOI][PubMed]
  • 31. Cabral AB, Melo Rde C, Maciel MA, Lopes AC. Multidrug resistance genes, including bla(KPC) and bla(CTX)-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil. Rev Soc Bras Med Trop. 2012; 45(5): 572-8[PubMed]
  • 32. Diago-Navarro E, Chen L, Passet V, Burack S, Ulacia-Hernando A, Kodiyanplakkal RP, et al. Carbapenem-resistant Klebsiella pneumoniae exhibit variability in capsular polysaccharide and capsule associated virulence traits. J Infect Dis. 2014; 210(5): 803-13[DOI][PubMed]
  • 33. Vassena C, Fenu S, Giuliani F, Fantetti L, Roncucci G, Simonutti G, et al. Photodynamic antibacterial and antibiofilm activity of RLP068/Cl against Staphylococcus aureus and Pseudomonas aeruginosa forming biofilms on prosthetic material. Int J Antimicrob Agents. 2014; 44(1): 47-55[DOI][PubMed]
  • 34. Kazemian H, Ghafourian S, Heidari H, Amiri P, Yamchi JK, Shavalipour A, et al. Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa. Rev Soc Bras Med Trop. 2015; 48(4): 432-6[DOI][PubMed]
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