Jundishapur Journal of Microbiology

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Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran

Maryam Sadeh 1 , Roya Firouzi 1 , Abdollah Derakhshandeh 1 , * , Mohammad Bagher Khalili 2 , Fanrong Kong 3 and Timothy Kudinha 4
Authors Information
1 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IR Iran
2 School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran
3 Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR-Pathology West, Westmead Hospital, University of Sydney, New South Wales 2145, Australia
4 Charles Sturt University, New South Wales, Australia
Article information
  • Jundishapur Journal of Microbiology: February 01, 2016, 9 (2); e30412
  • Published Online: February 15, 2016
  • Article Type: Research Article
  • Received: May 31, 2015
  • Revised: November 28, 2015
  • Accepted: November 28, 2015
  • DOI: 10.5812/jjm.30412

To Cite: Sadeh M, Firouzi R, Derakhshandeh A, Bagher Khalili M, Kong F, et al. Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran, Jundishapur J Microbiol. 2016 ; 9(2):e30412. doi: 10.5812/jjm.30412.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Martins ER, Melo-Cristino J, Ramirez M. Evidence for rare capsular switching in Streptococcus agalactiae. J Bacteriol. 2010; 192(5): 1361-9[DOI][PubMed]
  • 2. Gosiewski T, Brzychczy-Wloch M, Heczko PB. The application of multiplex PCR to detect seven different DNA targets in group B streptococci. Folia Microbiol . 2012; 57(3): 163-7[DOI][PubMed]
  • 3. Lindahl G, Stalhammar-Carlemalm M, Areschoug T. Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens. Clin Microbiol Rev. 2005; 18(1): 102-27[DOI][PubMed]
  • 4. Rabiei S, Arab M, Yousefi MR. Epidemiologic pattern of vaginal colonization by group B Streptococcus in pregnant women in Hamadan, Central west of Iran. IJMS. 2006; 31(2): 106-8
  • 5. Johri AK, Paoletti LC, Glaser P, Dua M, Sharma PK, Grandi G, et al. Group B Streptococcus: global incidence and vaccine development. Nat Rev Microbiol. 2006; 4(12): 932-42[DOI][PubMed]
  • 6. Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL. Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J Clin Microbiol. 2007; 45(9): 2929-36[DOI][PubMed]
  • 7. Ho YR, Li CM, Su HP, Wu JH, Tseng YC, Lin YJ, et al. Variation in the number of tandem repeats and profile of surface protein genes among invasive group B Streptococci correlates with patient age. J Clin Microbiol. 2007; 45(5): 1634-6[DOI][PubMed]
  • 8. Mavenyengwa RT, Maeland JA, Moyo SR. Distinctive features of surface-anchored proteins of Streptococcus agalactiae strains from Zimbabwe revealed by PCR and dot blotting. Clin Vaccine Immunol. 2008; 15(9): 1420-4
  • 9. Radtke A, Kong F, Bergh K, Lyng RV, Ko D, Gilbert GL. Identification of surface proteins of group B streptococci: serotyping versus genotyping. J Microbiol Methods. 2009; 78(3): 363-5[DOI][PubMed]
  • 10. Lubas MB, Wloch MB, Adamski P, Gosiewski T, Strus M, Heczko PB. Adherence of Group B Streptococci to Human Rectal and Vaginal Epithelial Cell Lines in relation to Capsular Polysaccharides as well as Alpha-like Protein Genes–Pilot Study. Pol J Microbiol. 2013; 62(1): 85-90
  • 11. Kong F, Gowan S, Martin D, James G, Gilbert GL. Serotype identification of group B streptococci by PCR and sequencing. J Clin Microbiol. 2002; 40(1): 216-26[PubMed]
  • 12. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis. 2010; 10: 336[DOI][PubMed]
  • 13. Lee HR, Song SH, Kim HB, Park KU, Song J. A rapid genotyping test for the simultaneous detection and subtyping of group B streptococci: the frequency of molecular subtypes of group B streptococci in Korea. Eur J Clin Microbiol Infect Dis. 2010; 29(10): 1287-90[DOI][PubMed]
  • 14. Poyart C, Tazi A, Reglier-Poupet H, Billoet A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol. 2007; 45(6): 1985-8[DOI][PubMed]
  • 15. Zhao Z, Kong F, Martinez G, Zeng X, Gottschalk M, Gilbert GL. Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay. FEMS Microbiol Lett. 2006; 263(2): 236-9[DOI][PubMed]
  • 16. Kong F, Ma L, Gilbert GL. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. J Med Microbiol. 2005; 54: 1133-8[DOI][PubMed]
  • 17. Creti R, Fabretti F, Orefici G, von Hunolstein C. Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes. Eur J Clin Microbiol. 2004; 42(3): 1326-9[PubMed]
  • 18. Absalan M, Eslami , Zandi H, Mosaddegh A, Vakili M, Khalili MB. Prevalence of Recto-Vaginal Colonization of Group B Streptococcus in Pregnant Women. JIMS. 2013; 30(220): 2367-75
  • 19. Beigverdi R, Jabalameli F, Mirsalehian A, Hantoushzadeh S, Boroumandi S, Taherikalani M, et al. Virulence factors, antimicrobial susceptibility and molecular characterization of Streptococcus agalactiae isolated from pregnant women. Acta Microbiol Immunol Hung. 2014; 61(4): 425-34[DOI][PubMed]
  • 20. Cieslewicz MJ, Chaffin D, Glusman G, Kasper D, Madan A, Rodrigues S, et al. Structural and genetic diversity of group B streptococcus capsular polysaccharides. Infect Immun. 2005; 73(5): 3096-103[DOI][PubMed]
  • 21. Kvam AI, Mavenyengwa RT, Radtke A, Maeland JA. Streptococcus agalactiae alpha-like protein 1 possesses both cross-reacting and Alp1-specific epitopes. Clin Vaccine Immunol. 2011; 18(8): 1365-70[DOI][PubMed]
  • 22. Glaser P, Rusniok C, Buchrieser C, Chevalier F, Frangeul L, Msadek T, et al. Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease. Mol Microbiol. 2002; 45(6): 1499-513[PubMed]
  • 23. Sadowy E, Matynia B, Hryniewicz W. Population structure, virulence factors and resistance determinants of invasive, non-invasive and colonizing Streptococcus agalactiae in Poland. J Antimicrob Chemother. 2010; 65(9): 1907-14[DOI][PubMed]
  • 24. Brimil N, Barthell E, Heindrichs U, Kuhn M, Lutticken R, Spellerberg B. Epidemiology of Streptococcus agalactiae colonization in Germany. Int J Med Microbiol. 2006; 296(1): 39-44[DOI][PubMed]
  • 25. Meehan M, Cunney R, Cafferkey M. Molecular epidemiology of group B streptococci in Ireland reveals a diverse population with evidence of capsular switching. Eur J Clin Microbiol Infect Dis. 2014; 33(7): 1155-62[DOI][PubMed]
  • 26. De Francesco MA, Caracciolo S, Gargiulo F, Manca N. Phenotypes, genotypes, serotypes and molecular epidemiology of erythromycin-resistant Streptococcus agalactiae in Italy. Eur J Clin Microbiol Infect Dis. 2012; 31(8): 1741-7[DOI][PubMed]
  • 27. Kong F, Gowan S, Martin D, James G, Gilbert GL. Molecular profiles of group B streptococcal surface protein antigen genes: relationship to molecular serotypes. J Clin Microbiol. 2002; 40(2): 620-6[PubMed]
  • 28. Seoud M, Nassar AH, Zalloua P, Boghossian N, Ezeddine J, Fakhoury H, et al. Prenatal and neonatal Group B Streptococcus screening and serotyping in Lebanon: incidence and implications. Acta Obstet Gynecol Scand. 2010; 89(3): 399-403[DOI][PubMed]
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