Jundishapur Journal of Microbiology

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Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes

Mohammad Mousavi 1 , 2 , Ramin Saravani 3 , 4 , * , Mohammad Jafari Modrek 1 , 2 , Mahnaz Shahrakipour 5 , 6 and Sina Sekandarpour 2
Authors Information
1 Infectious and Tropical Medicine Research Center, Zahedan, IR Iran
2 Department of Medical Parasitology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
3 Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
4 Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
5 Pregnancy Help Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
6 Department of Biostatistics and Epidemiology, School of Health, Zahedan University of Medical Sciences, Zahedan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: February 01, 2016, 9 (2); e29493
  • Published Online: February 9, 2016
  • Article Type: Research Article
  • Received: June 1, 2015
  • Revised: November 25, 2015
  • Accepted: November 30, 2015
  • DOI: 10.5812/jjm.29493

To Cite: Mousavi M, Saravani R, Jafari Modrek M, Shahrakipour M, Sekandarpour S. Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes, Jundishapur J Microbiol. 2016 ; 9(2):e29493. doi: 10.5812/jjm.29493.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Footnotes
References
  • 1. Carruthers VB. Host cell invasion by the opportunistic pathogen Toxoplasma gondii. Acta Tropica. 2002; 81(2): 111-22[DOI][PubMed]
  • 2. Montoya JG, Liesenfeld O. Toxoplasmosis. The Lancet. 2004; 363(9425): 1965-76[DOI]
  • 3. Webster JP, Kaushik M, Bristow GC, McConkey GA. Toxoplasma gondii infection, from predation to schizophrenia: can animal behaviour help us understand human behaviour? J Exp Biol. 2013; 216: 99-112[DOI][PubMed]
  • 4. Shirbazou S, Delpisheh A, Mokhetari R, Tavakoli G. Serologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients. Iran Red Crescent Med J. 2013; 15(8): 701-3[DOI][PubMed]
  • 5. Gokce C, Yazar S, Bayram F, Gundogan K, Yaman O, Sahin I. Anti-Toxoplasma gondii antibodies in type 2 diabetes. Natl Med J India. 2008; 21(1): 51[PubMed]
  • 6. Goekce C, Yazar S, Bayram F, Gundogan K. Toxoplasma gondii antibodies in type 1 diabetes mellitus. Turkiye Klinikleri J. 2008; 28(5): 619-22
  • 7. Lau YL, Meganathan P, Sonaimuthu P, Thiruvengadam G, Nissapatorn V, Chen Y. Specific, sensitive, and rapid diagnosis of active toxoplasmosis by a loop-mediated isothermal amplification method using blood samples from patients. J Clin Microbiol. 2010; 48(10): 3698-702[DOI][PubMed]
  • 8. Zhang H, Thekisoe OM, Aboge GO, Kyan H, Yamagishi J, Inoue N, et al. Toxoplasma gondii: sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method. Exp Parasitol. 2009; 122(1): 47-50[DOI][PubMed]
  • 9. Anand R, Jones CW, Ricks JH, Sofarelli TA, Hale DC. Acute primary toxoplasmosis in travelers returning from endemic countries. J Travel Med. 2012; 19(1): 57-60[DOI][PubMed]
  • 10. Cassaing S, Bessieres MH, Berry A, Berrebi A, Fabre R, Magnaval JF. Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR. J Clin Microbiol. 2006; 44(3): 720-4[DOI][PubMed]
  • 11. Al-Kappany YM, Rajendran C, Abu-Elwafa SA, Hilali M, Su C, Dubey JP. Genetic diversity of Toxoplasma gondii isolates in Egyptian feral cats reveals new genotypes. J Parasitol. 2010; 96(6): 1112-4[DOI][PubMed]
  • 12. Homan WL, Vercammen M, De Braekeleer J, Verschueren H. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR1Note: Nucleotide sequence data reported in this paper have been submitted to GenBankTM database with the accession number AF146527 (Toxoplasma gondii genomic repetitive 529 bp fragment).1. International Journal for Parasitology. 2000; 30(1): 69-75[DOI][PubMed]
  • 13. Fallahi S, Kazemi B, Seyyed tabaei SJ, Bandehpour M, Lasjerdi Z, Taghipour N, et al. Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer. Parasitol Int. 2014; 63(1): 37-41[DOI][PubMed]
  • 14. Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol. 1989; 27(8): 1787-92[PubMed]
  • 15. Costa JM, Bretagne S. Variation of B1 gene and AF146527 repeat element copy numbers according to Toxoplasma gondii strains assessed using real-time quantitative PCR. J Clin Microbiol. 2012; 50(4): 1452-4[DOI][PubMed]
  • 16. Filisetti D, Gorcii M, Pernot-Marino E, Villard O, Candolfi E. Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR. J Clin Microbiol. 2003; 41(10): 4826-8[PubMed]
  • 17. Reischl U, Bretagne S, Kruger D, Ernault P, Costa JM. Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis. 2003; 3: 7[PubMed]
  • 18. Wahab T, Edvinsson B, Palm D, Lindh J. Comparison of the AF146527 and B1 repeated elements, two real-time PCR targets used for detection of Toxoplasma gondii. J Clin Microbiol. 2010; 48(2): 591-2[DOI][PubMed]
  • 19. Mahittikorn A, Wickert H, Sukthana Y. Comparison of five DNA extraction methods and optimization of a b1 gene nested PCR (nPCR) for detection of Toxoplasma gondii tissue cyst in mouse brain. Southeast Asian J Trop Med Public Health. 2005; 36(6): 1377-82[PubMed]
  • 20. Modrek MJ, Saravani R, Mousavi M, Khorashad AS, Piri M. Investigation of IgG and IgM Antibodies Against Toxoplasma gondii Among Diabetic Patients. Int J Infect. 2015; 2(3)[DOI]
  • 21. Saremi AM, Saremi M, Tavallaei M. Rapid genomic DNA extraction (RGDE). Forensic Sci Int Genet. 2008; 1(1): 63-5[DOI]
  • 22. Tonkal AM. PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia. J Egypt Soc Parasitol. 2008; 38(3): 707-14[PubMed]
  • 23. Yamada H, Nishikawa A, Yamamoto T, Mizue Y, Yamada T, Morizane M, et al. Prospective study of congenital toxoplasmosis screening with use of IgG avidity and multiplex nested PCR methods. J Clin Microbiol. 2011; 49(7): 2552-6[DOI][PubMed]
  • 24. Calderaro A, Piccolo G, Gorrini C, Peruzzi S, Zerbini L, Bommezzadri S, et al. Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii. Acta Biomed. 2006; 77(2): 75-80[PubMed]
  • 25. Sanchez JF, Olmedo MC, Pascua FJ, Casado I. Diabetes insipidus as a manifestation of cerebral toxoplasmosis in an AIDS patient. Rev Neurol. 2000; 30(10): 939-40[PubMed]
  • 26. Herrmann DC, Maksimov P, Hotop A, Gross U, Daubener W, Liesenfeld O, et al. Genotyping of samples from German patients with ocular, cerebral and systemic toxoplasmosis reveals a predominance of Toxoplasma gondii type II. Int J Med Microbiol. 2014; 304(7): 911-6[DOI][PubMed]
  • 27. Jones CD, Okhravi N, Adamson P, Tasker S, Lightman S. Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor. Invest Ophthalmol Vis Sci. 2000; 41(3): 634-44[PubMed]
  • 28. Cardona N, Basto N, Parra B, Zea AF, Pardo CA, Bonelo A, et al. Detection of Toxoplasma DNA in the peripheral blood of HIV-positive patients with neuro-opportunistic infections by a Real-Time PCR assay. J Neuroparasitol. 2011; 2: 1-6
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