Jundishapur Journal of Microbiology

Published by: Kowsar

Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran

Bentolhoda Zahraei 1 , Mohammad Sadegh Hashemzadeh 1 , Mohammad Najarasl 1 , Samaneh Zahiriyeganeh 1 , Mahdi Tat 1 , Maliheh Metanat 2 , Nahid Sepehri Rad 2 , Behzad Khansari-nejad 3 , Ehsan Zafari 1 , Mojtaba Sharti 1 and Ruhollah Dorostkar 1 , *
Authors Information
1 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Research Center for Infectious Diseases and Tropical Medicine, Boo-Ali Hospital, Zahedan University of Medical Sciences, Zahedan, IR Iran
3 Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 01, 2016, 9 (1); e57165
  • Published Online: January 2, 2016
  • Article Type: Research Article
  • Received: April 17, 2015
  • Revised: July 6, 2015
  • Accepted: July 25, 2015
  • DOI: 10.5812/jjm.29246

To Cite: Zahraei B, Hashemzadeh M S, Najarasl M, Zahiriyeganeh S, Tat M, et al. Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran, Jundishapur J Microbiol. 2016 ; 9(1):e57165. doi: 10.5812/jjm.29246.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Vazirianzadeh B, Rahdar M. Correct identification of animal host species is important in the diagnosis of Zoonotic diseases. Jundishapur J Microbiol. 2013; 6(2): 97-9[DOI]
  • 2. Chinikar S, Ghiasi SM, Hewson R, Moradi M, Haeri A. Crimean-Congo hemorrhagic fever in Iran and neighboring countries. J Clin Virol. 2010; 47(2): 110-4[DOI][PubMed]
  • 3. Ergonul O. Crimean-Congo haemorrhagic fever. Lancet. 2006; 6(4): 203-14[DOI][PubMed]
  • 4. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, et al. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol. 2002; 40(7): 2323-30[PubMed]
  • 5. Estrada-Pena A, Vatansever Z, Gargili A, Buzgan T. An early warning system for Crimean-Congo haemorrhagic fever seasonality in Turkey based on remote sensing technology. Geospat Health. 2007; 2(1): 127-35[DOI][PubMed]
  • 6. Vorou RM. Crimean-Congo hemorrhagic fever in southeastern Europe. Int J Infect Dis. 2009; 13(6): 659-62[DOI][PubMed]
  • 7. Mostafavi E, Chinikar S, Bokaei S, Haghdoost A. Temporal modeling of Crimean-Congo hemorrhagic fever in eastern Iran. Int J Infect Dis. 2013; 17(7)-8[DOI][PubMed]
  • 8. Chinikar S, Mojtaba Ghiasi S, Moradi M, Goya MM, Reza Shirzadi M, Zeinali M, et al. Phylogenetic analysis in a recent controlled outbreak of Crimean-Congo haemorrhagic fever in the south of Iran, December 2008. Euro Surveill. 2010; 15(47): 1-4
  • 9. Chinikar S, Ghiasi SM, Moradi M, Goya MM, Shirzadi MR, Zeinali M, et al. Geographical distribution and surveillance of Crimean-Congo hemorrhagic fever in Iran. Vector Borne Zoonotic Dis. 2010; 10(7): 705-8[DOI][PubMed]
  • 10. Izadi S, Holakouie-Naieni K, Majdzadeh SR, Chinikar S, Nadim A, Rakhshani F, et al. Seroprevalence of Crimean-Congo hemorrhagic fever in Sistan-va-Baluchestan province of Iran. Jpn J Infect Dis. 2006; 59(5): 326-8[PubMed]
  • 11. Izadi S, Naieni KH, Madjdzadeh SR, Nadim A. Crimean-Congo hemorrhagic fever in Sistan and Baluchestan Province of Iran, a case-control study on epidemiological characteristics. Int J Infect Dis. 2004; 8(5): 299-306[DOI][PubMed]
  • 12. Naieni KH, Izadi SH, Chinikar S, Nadim A. Seroprevalence, incidence and risk factors of Crimean-Congo hemorrhagic fever in Sistan-va-Baluchestan province, Iran. Iran J Public Health. 2004; 33(4): 1-7
  • 13. Alavi-Naini R, Moghtaderi A, Koohpayeh HR, Sharifi-Mood B, Naderi M, Metanat M, et al. Crimean-Congo hemorrhagic fever in Southeast of Iran. J Infect. 2006; 52(5): 378-82[DOI][PubMed]
  • 14. Chinikar S, Persson SM, Johansson M, Bladh L, Goya M, Houshmand B, et al. Genetic analysis of Crimean-congo hemorrhagic fever virus in Iran. J Med Virol. 2004; 73(3): 404-11[DOI][PubMed]
  • 15. Raziei T, Daneshkar A. P. , Akhtari R, Saghafian B. Investigation of meteorological droughts in the Sistan and Balouchestan province, Using the standardized precipitation index and Markov chain model. Water Resour Res. 2007; 3(1): 25-35
  • 16. Fajs L, Jakupi X, Ahmeti S, Humolli I, Dedushaj I, Avsic-Zupanc T. Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Kosovo. PLoS Negl Trop Dis. 2014; 8(1)[DOI][PubMed]
  • 17. Fukushima H, Tsunomori Y, Seki R. Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools. J Clin Microbiol. 2003; 41(11): 5134-46[PubMed]
  • 18. Altamura LA, Bertolotti-Ciarlet A, Teigler J, Paragas J, Schmaljohn CS, Doms RW. Identification of a novel C-terminal cleavage of Crimean-Congo hemorrhagic fever virus PreGN that leads to generation of an NSM protein. J Virol. 2007; 81(12): 6632-42[DOI][PubMed]
  • 19. Mehravaran A, Moradi M, Telmadarraiy Z, Mostafavi E, Moradi AR, Khakifirouz S, et al. Molecular detection of Crimean-Congo haemorrhagic fever (CCHF) virus in ticks from southeastern Iran. Ticks Tick Borne Dis. 2013; 4(1-2): 35-8[DOI][PubMed]
  • 20. Chinikar S, Goya MM, Shirzadi MR, Ghiasi SM, Mirahmadi R, Haeri A, et al. Surveillance and laboratory detection system of Crimean-Congo haemorrhagic fever in Iran. Transbound Emerg Dis. 2008; 55(5-6): 200-4[DOI][PubMed]
  • 21. Fakoorziba MR, Golmohammadi P, Moradzadeh R, Moemenbellah-Fard MD, Azizi K, Davari B, et al. Reverse transcription PCR-based detection of Crimean-Congo hemorrhagic fever virus isolated from ticks of domestic ruminants in Kurdistan province of Iran. Vector Borne Zoonotic Dis. 2012; 12(9): 794-9[DOI][PubMed]
  • 22. Jothikumar N, Griffiths MW. Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays. Appl Environ Microbiol. 2002; 68(6): 3169-71[PubMed]
  • 23. Maltezou HC, Andonova L, Andraghetti R, Bouloy M, Ergonul O, Jongejan F, et al. Crimean-Congo hemorrhagic fever in Europe: Current situation calls for preparedness. Euro Surveill. 2010; 15(10): 19504[PubMed]
  • 24. Morikawa S, Saijo M, Kurane I. Recent progress in molecular biology of Crimean-Congo hemorrhagic fever. Comp Immunol Microbiol Infect Dis. 2007; 30(5-6): 375-89[DOI][PubMed]
  • 25. Whitehouse CA. Crimean-Congo hemorrhagic fever. Antiviral Res. 2004; 64(3): 145-60[DOI][PubMed]
  • 26. Zhu Z, Fan H, Qi X, Qi Y, Shi Z, Wang H, et al. Development and evaluation of a SYBR green-based real time RT-PCR assay for detection of the emerging avian influenza A (H7N9) virus. PLoS One. 2013; 8(11)[DOI][PubMed]
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