Jundishapur Journal of Microbiology

Published by: Kowsar

Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran

Bentolhoda Zahraei 1 , Mohammad Sadegh Hashemzadeh 1 , Mohammad Najarasl 1 , Samaneh Zahiriyeganeh 1 , Mahdi Tat 1 , Maliheh Metanat 2 , Nahid Sepehri Rad 2 , Behzad Khansari-nejad 3 , Ehsan Zafari 1 , Mojtaba Sharti 1 and Ruhollah Dorostkar 1 , *
Authors Information
1 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Research Center for Infectious Diseases and Tropical Medicine, Boo-Ali Hospital, Zahedan University of Medical Sciences, Zahedan, IR Iran
3 Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 01, 2016, 9 (1); e57165
  • Published Online: January 2, 2016
  • Article Type: Research Article
  • Received: April 17, 2015
  • Revised: July 6, 2015
  • Accepted: July 25, 2015
  • DOI: 10.5812/jjm.29246

To Cite: Zahraei B, Hashemzadeh M S, Najarasl M, Zahiriyeganeh S, Tat M, et al. Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran, Jundishapur J Microbiol. 2016 ; 9(1):e57165. doi: 10.5812/jjm.29246.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Vazirianzadeh B, Rahdar M. Correct identification of animal host species is important in the diagnosis of Zoonotic diseases. Jundishapur J Microbiol. 2013; 6(2): 97-9[DOI]
  • 2. Chinikar S, Ghiasi SM, Hewson R, Moradi M, Haeri A. Crimean-Congo hemorrhagic fever in Iran and neighboring countries. J Clin Virol. 2010; 47(2): 110-4[DOI][PubMed]
  • 3. Ergonul O. Crimean-Congo haemorrhagic fever. Lancet. 2006; 6(4): 203-14[DOI][PubMed]
  • 4. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, et al. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol. 2002; 40(7): 2323-30[PubMed]
  • 5. Estrada-Pena A, Vatansever Z, Gargili A, Buzgan T. An early warning system for Crimean-Congo haemorrhagic fever seasonality in Turkey based on remote sensing technology. Geospat Health. 2007; 2(1): 127-35[DOI][PubMed]
  • 6. Vorou RM. Crimean-Congo hemorrhagic fever in southeastern Europe. Int J Infect Dis. 2009; 13(6): 659-62[DOI][PubMed]
  • 7. Mostafavi E, Chinikar S, Bokaei S, Haghdoost A. Temporal modeling of Crimean-Congo hemorrhagic fever in eastern Iran. Int J Infect Dis. 2013; 17(7)-8[DOI][PubMed]
  • 8. Chinikar S, Mojtaba Ghiasi S, Moradi M, Goya MM, Reza Shirzadi M, Zeinali M, et al. Phylogenetic analysis in a recent controlled outbreak of Crimean-Congo haemorrhagic fever in the south of Iran, December 2008. Euro Surveill. 2010; 15(47): 1-4
  • 9. Chinikar S, Ghiasi SM, Moradi M, Goya MM, Shirzadi MR, Zeinali M, et al. Geographical distribution and surveillance of Crimean-Congo hemorrhagic fever in Iran. Vector Borne Zoonotic Dis. 2010; 10(7): 705-8[DOI][PubMed]
  • 10. Izadi S, Holakouie-Naieni K, Majdzadeh SR, Chinikar S, Nadim A, Rakhshani F, et al. Seroprevalence of Crimean-Congo hemorrhagic fever in Sistan-va-Baluchestan province of Iran. Jpn J Infect Dis. 2006; 59(5): 326-8[PubMed]
  • 11. Izadi S, Naieni KH, Madjdzadeh SR, Nadim A. Crimean-Congo hemorrhagic fever in Sistan and Baluchestan Province of Iran, a case-control study on epidemiological characteristics. Int J Infect Dis. 2004; 8(5): 299-306[DOI][PubMed]
  • 12. Naieni KH, Izadi SH, Chinikar S, Nadim A. Seroprevalence, incidence and risk factors of Crimean-Congo hemorrhagic fever in Sistan-va-Baluchestan province, Iran. Iran J Public Health. 2004; 33(4): 1-7
  • 13. Alavi-Naini R, Moghtaderi A, Koohpayeh HR, Sharifi-Mood B, Naderi M, Metanat M, et al. Crimean-Congo hemorrhagic fever in Southeast of Iran. J Infect. 2006; 52(5): 378-82[DOI][PubMed]
  • 14. Chinikar S, Persson SM, Johansson M, Bladh L, Goya M, Houshmand B, et al. Genetic analysis of Crimean-congo hemorrhagic fever virus in Iran. J Med Virol. 2004; 73(3): 404-11[DOI][PubMed]
  • 15. Raziei T, Daneshkar A. P. , Akhtari R, Saghafian B. Investigation of meteorological droughts in the Sistan and Balouchestan province, Using the standardized precipitation index and Markov chain model. Water Resour Res. 2007; 3(1): 25-35
  • 16. Fajs L, Jakupi X, Ahmeti S, Humolli I, Dedushaj I, Avsic-Zupanc T. Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Kosovo. PLoS Negl Trop Dis. 2014; 8(1)[DOI][PubMed]
  • 17. Fukushima H, Tsunomori Y, Seki R. Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools. J Clin Microbiol. 2003; 41(11): 5134-46[PubMed]
  • 18. Altamura LA, Bertolotti-Ciarlet A, Teigler J, Paragas J, Schmaljohn CS, Doms RW. Identification of a novel C-terminal cleavage of Crimean-Congo hemorrhagic fever virus PreGN that leads to generation of an NSM protein. J Virol. 2007; 81(12): 6632-42[DOI][PubMed]
  • 19. Mehravaran A, Moradi M, Telmadarraiy Z, Mostafavi E, Moradi AR, Khakifirouz S, et al. Molecular detection of Crimean-Congo haemorrhagic fever (CCHF) virus in ticks from southeastern Iran. Ticks Tick Borne Dis. 2013; 4(1-2): 35-8[DOI][PubMed]
  • 20. Chinikar S, Goya MM, Shirzadi MR, Ghiasi SM, Mirahmadi R, Haeri A, et al. Surveillance and laboratory detection system of Crimean-Congo haemorrhagic fever in Iran. Transbound Emerg Dis. 2008; 55(5-6): 200-4[DOI][PubMed]
  • 21. Fakoorziba MR, Golmohammadi P, Moradzadeh R, Moemenbellah-Fard MD, Azizi K, Davari B, et al. Reverse transcription PCR-based detection of Crimean-Congo hemorrhagic fever virus isolated from ticks of domestic ruminants in Kurdistan province of Iran. Vector Borne Zoonotic Dis. 2012; 12(9): 794-9[DOI][PubMed]
  • 22. Jothikumar N, Griffiths MW. Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays. Appl Environ Microbiol. 2002; 68(6): 3169-71[PubMed]
  • 23. Maltezou HC, Andonova L, Andraghetti R, Bouloy M, Ergonul O, Jongejan F, et al. Crimean-Congo hemorrhagic fever in Europe: Current situation calls for preparedness. Euro Surveill. 2010; 15(10): 19504[PubMed]
  • 24. Morikawa S, Saijo M, Kurane I. Recent progress in molecular biology of Crimean-Congo hemorrhagic fever. Comp Immunol Microbiol Infect Dis. 2007; 30(5-6): 375-89[DOI][PubMed]
  • 25. Whitehouse CA. Crimean-Congo hemorrhagic fever. Antiviral Res. 2004; 64(3): 145-60[DOI][PubMed]
  • 26. Zhu Z, Fan H, Qi X, Qi Y, Shi Z, Wang H, et al. Development and evaluation of a SYBR green-based real time RT-PCR assay for detection of the emerging avian influenza A (H7N9) virus. PLoS One. 2013; 8(11)[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments