Jundishapur Journal of Microbiology

Published by: Kowsar

Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens

Roohollah Zarei Koosha 1 , 2 , Hamideh Mahmoodzadeh Hosseini 1 , Elnaz Mehdizadeh Aghdam 3 , Shafie Ghorbani Tajandareh 1 and Abbas Ali Imani Fooladi 1 , *
Authors Information
1 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Biology Department, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran
3 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: March 01, 2016, 9 (3); e29057
  • Published Online: March 12, 2016
  • Article Type: Research Article
  • Received: April 14, 2015
  • Revised: October 27, 2015
  • Accepted: December 19, 2015
  • DOI: 10.5812/jjm.29057

To Cite: Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, Ghorbani Tajandareh S, Imani Fooladi A A. Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens, Jundishapur J Microbiol. 2016 ; 9(3):e29057. doi: 10.5812/jjm.29057.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Deurenberg RH, Nieuwenhuis RF, Driessen C, London N, Stassen FR, van Tiel FH, et al. The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients. FEMS Microbiol Lett. 2005; 245(1): 185-9[DOI][PubMed]
  • 2. Fraser J, Arcus V, Kong P, Baker E, Proft T. Superantigens – powerful modifiers of the immune system. Mol Med Today. 2000; 6(3): 125-32[DOI][PubMed]
  • 3. Llewelyn M, Cohen J. Superantigens: microbial agents that corrupt immunity. The Lancet Infectious Diseases. 2002; 2(3): 156-62[DOI][PubMed]
  • 4. Durand G, Bes M, Meugnier H, Enright MC, Forey F, Liassine N, et al. Detection of new methicillin-resistant Staphylococcus aureus clones containing the toxic shock syndrome toxin 1 gene responsible for hospital- and community-acquired infections in France. J Clin Microbiol. 2006; 44(3): 847-53[DOI][PubMed]
  • 5. Tristan A, Ferry T, Durand G, Dauwalder O, Bes M, Lina G, et al. Virulence determinants in community and hospital meticillin-resistant Staphylococcus aureus. J Hosp Infect . 2007; 65: 105-9[DOI][PubMed]
  • 6. Parsonnet J, Goering RV, Hansmann MA, Jones MB, Ohtagaki K, Davis CC, et al. Prevalence of toxic shock syndrome toxin 1 (TSST-1)-producing strains of Staphylococcus aureus and antibody to TSST-1 among healthy Japanese women. J Clin Microbiol. 2008; 46(8): 2731-8[DOI][PubMed]
  • 7. Wikler MA. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard—Eighth Edition. 2009;
  • 8. Imani Fouladi A, Choupani A, Fallah Mehrabadi J. Study of prevalence of Enterotoxin type B gene in Meticillin Resistant Staphylococcus aureus (MRSA) isolated from wound. Kowsar Med J. 2011; 16(1): 21-5
  • 9. Gill SR, Fouts DE, Archer GL, Mongodin EF, Deboy RT, Ravel J, et al. Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol. 2005; 187(7): 2426-38[DOI][PubMed]
  • 10. Peng Q, Hou B, Zhou S, Huang Y, Hua D, Yao F, et al. Staphylococcal cassette chromosome mec (SCCmec) analysis and antimicrobial susceptibility profiles of methicillin-resistant Staphylococcus aureus (MRSA) isolates in a teaching hospital, Shantou, China. Afr J Microbiol Res. 2010; 4(9): 844-8
  • 11. Tokajian S, Haddad D, Andraos R, Hashwa F, Araj G. Toxins and Antibiotic Resistance in Staphylococcus aureus Isolated from a Major Hospital in Lebanon. ISRN Microbiology. 2011; 2011: 1-9[DOI]
  • 12. de Neeling AJ, van Leeuwen WJ, Schouls LM, Schot CS, van Veen-Rutgers A, Beunders AJ, et al. Resistance of staphylococci in The Netherlands: surveillance by an electronic network during 1989-1995. J Antimicrob Chemother. 1998; 41(1): 93-101[PubMed]
  • 13. Voss A, Milatovic D, Wallrauch-Schwarz C, Rosdahl VT, Braveny I. Methicillin-resistant Staphylococcus aureus in Europe. Eur J Clin Microbiol Infect Dis. 1994; 13(1): 50-5[PubMed]
  • 14. Gbaguidi-Haore H, Thouverez M, Couetdic G, Cholley P, Talon D, Bertrand X. Usefulness of antimicrobial resistance pattern for detecting PVL- or TSST-1-producing meticillin-resistant Staphylococcus aureus in a French university hospital. J Med Microbiol. 2009; 58: 1337-42[DOI][PubMed]
  • 15. Yu F, Li T, Huang X, Xie J, Xu Y, Tu J, et al. Virulence gene profiling and molecular characterization of hospital-acquired Staphylococcus aureus isolates associated with bloodstream infection. Diagn Microbiol Infect Dis. 2012; 74(4): 363-8[DOI][PubMed]
  • 16. Shittu AO, Okon K, Adesida S, Oyedara O, Witte W, Strommenger B, et al. Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria. BMC Microbiol. 2011; 11: 92[DOI][PubMed]
  • 17. Vorobieva V, Bazhukova T, Hanssen AM, Caugant DA, Semenova N, Haldorsen BC, et al. Clinical isolates of Staphylococcus aureus from the Arkhangelsk region, Russia: antimicrobial susceptibility, molecular epidemiology, and distribution of Panton-Valentine leukocidin genes. APMIS. 2008; 116(10): 877-87[DOI][PubMed]
  • 18. Rahimi F, Bouzari M, Maleki Z, Rahimi F. Antibiotic susceptibility pattern among Staphylococcus spp. with emphasis on detection of mecA gene in methicillin resistant Staphylococcus aureus isolates. Arch Clin Infect Dis. 2009; 4(3): 143-50
  • 19. Shahkarami F, Rashki A, Rashki Ghalehnoo Z. Microbial Susceptibility and Plasmid Profiles of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible S. aureus. Jundishapur J Microbiol. 2014; 7(7)[DOI][PubMed]
  • 20. El-Ghodban A, Ghenghesh KS, Marialigeti K, Esahli H, Tawil A. PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya. J Med Microbiol. 2006; 55: 179-82[DOI][PubMed]
  • 21. Tsen HY, Yu GK, Wang KC, Wang SJ, Chang MY, Lin LY. Comparison of the enterotoxigenic types, toxic shock syndrome toxin I (TSST-1) strains and antibiotic susceptibilities for enterotoxigenicStaphylococcus aureusstrains isolated from food and clinical samples. Food Microbiology. 1998; 15(1): 33-41[DOI]
  • 22. Demir C, Aslantas O, Duran N, Ocak S, Ozer B. Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital. Turk J Med Sci. 2011; 41(2): 343-52
  • 23. Liu M, Liu J, Guo Y, Zhang Z. Characterization of virulence factors and genetic background of Staphylococcus aureus isolated from Peking University People's Hospital between 2005 and 2009. Curr Microbiol. 2010; 61(5): 435-43[DOI][PubMed]
  • 24. Becker K, Friedrich AW, Lubritz G, Weilert M, Peters G, Von Eiff C. Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens. J Clin Microbiol. 2003; 41(4): 1434-9[PubMed]
  • 25. Fenner L, Widmer AF, Frei R. Molecular epidemiology of invasive methicillin-susceptible Staphylococcus aureus strains circulating at a Swiss University Hospital. Eur J Clin Microbiol Infect Dis. 2008; 27(7): 623-6[DOI][PubMed]
  • 26. Hoseini Alfatemi SM, Motamedifar M, Hadi N, Sedigh Ebrahim Saraie H. Analysis of Virulence Genes Among Methicillin Resistant Staphylococcus aureus (MRSA) Strains. Jundishapur J Microbiol. 2014; 7(6)[DOI][PubMed]
  • 27. Schlebusch S, Schooneveldt JM, Huygens F, Nimmo GR. Prevalence of Staphylococcus aureus strains in an Australian cohort, 1989-2003: evidence for the low prevalence of the toxic shock toxin and Panton-Valentine leukocidin genes. Eur J Clin Microbiol Infect Dis. 2009; 28(10): 1183-9[DOI][PubMed]
  • 28. Xie Y, He Y, Gehring A, Hu Y, Li Q, Tu SI, et al. Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolates from China. PLoS One. 2011; 6(12)[DOI][PubMed]
  • 29. Kimura A, Igarashi H, Ushioda H, Okuzumi K, Kobayashi H, Otsuka T. [Epidemiological study of Staphylococcus aureus isolated from the Japanese National University and Medical College Hospitals with coagulase typing, and production of enterotoxins and toxic shock syndrome toxin-1]. Kansenshogaku Zasshi. 1992; 66(11): 1543-9[PubMed]
  • 30. Lehn N, Schaller E, Wagner H, Kronke M. Frequency of toxic shock syndrome toxin- and enterotoxin-producing clinical isolates of Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1995; 14(1): 43-6[PubMed]
  • 31. Schmitz FJ, MacKenzie CR, Geisel R, Wagner S, Idel H, Verhoef J, et al. Enterotoxin and toxic shock syndrome toxin-1 production of methicillin resistant and methicillin sensitive Staphylococcus aureus strains. Eur J Epidemiol. 1997; 13(6): 699-708[PubMed]
  • 32. Jimenez JN, Ocampo AM, Vanegas JM, Rodriguez EA, Garces CG, Patino LA, et al. Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellin, Colombia. Mem Inst Oswaldo Cruz. 2011; 106(8): 980-5[PubMed]
  • 33. Shimaoka M, Yoh M, Takarada Y, Yamamoto K, Honda T. Detection of the gene for toxic shock syndrome toxin 1 in Staphylococcus aureus by enzyme-labelled oligonucleotide probes. J Med Microbiol. 1996; 44(3): 215-8[DOI][PubMed]
  • 34. Mohaghegh MA, Ghazvini K, Jafari R, Alikhani MY, Safari M, Azari Garamjan G, et al. Retrospective Study on the Prevalence and Antibiotic Resistance Pattern of Staphylococcus Aureus and Staphylococcus Epidermidis Among Patients Suspicious of Bacteremia During 2006 - 2011. International Journal of Enteric Pathogens. 2015; 3(2)[DOI]
  • 35. Maskell JP, Sefton AM, Williams JD. Comparative in-vitro activity of azithromycin and erymromycin against Gram-positive cocci, Haemophilus influenzae and anaerobes. J Antimicrob Chemother. 1990; 25: 19-24[PubMed]
  • 36. Okubo T, Iyobe S, Fujiki Y, Sagai H. [Antimicrobial activities of macrolides against recent clinical isolates, and analysis of resistant mechanisms]. Jpn J Antibiot. 2003; 56(3): 163-70[PubMed]
  • 37. Baddour MM, Abuelkheir MM, Fatani AJ. Trends in antibiotic susceptibility patterns and epidemiology of MRSA isolates from several hospitals in Riyadh, Saudi Arabia. Ann Clin Microbiol Antimicrob. 2006; 5: 30[DOI][PubMed]
  • 38. Maple P. World-Wide Antibiotic Resistance in Methicillin-Resistant Staphylococcus Aureus. The Lancet. 1989; 333(8637): 537-40[DOI]
  • 39. Nimmo GR, Bell JM, Mitchell D, Gosbell IB, Pearman JW, Turnidge JD, et al. Antimicrobial resistance in Staphylococcus aureus in Australian teaching hospitals, 1989-1999. Microb Drug Resist. 2003; 9(2): 155-60[DOI][PubMed]
  • 40. Hamze M, Dabboussi F, Daher W, Izard D. Résistance aux antibiotiques de Staphylococcus aureus au Nord du Liban : place de la résistance à la méticilline et comparaison des méthodes de détection. Pathologie Biologie. 2003; 51(1): 21-6[DOI][PubMed]
  • 41. Bischoff WE, Wallis ML, Tucker KB, Reboussin BA, Sherertz RJ. Staphylococcus aureus nasal carriage in a student community: prevalence, clonal relationships, and risk factors. Infect Control Hosp Epidemiol. 2004; 25(6): 485-91[DOI][PubMed]
  • 42. Grayson TH, Cooper LF, Atienzar FA, Knowles MR, Gilpin ML. Molecular differentiation of Renibacterium salmoninarum isolates from worldwide locations. Appl Environ Microbiol. 1999; 65(3): 961-8[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments