Jundishapur Journal of Microbiology

Published by: Kowsar

Pathotypic and Phylogenetic Study of Diarrheagenic Escherichia coli and Uropathogenic E. coli Using Multiplex Polymerase Chain Reaction

Hamzeh Salmani 1 , Asaad Azarnezhad 1 , Mohammad Reza Fayazi 1 and Arshad Hosseini 2 , *
Authors Information
1 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
2 Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: February 01, 2016, 9 (2); e28331
  • Published Online: February 2, 2016
  • Article Type: Research Article
  • Received: March 4, 2015
  • Revised: October 26, 2015
  • Accepted: October 29, 2015
  • DOI: 10.5812/jjm.28331

To Cite: Salmani H, Azarnezhad A, Fayazi M R, Hosseini A. Pathotypic and Phylogenetic Study of Diarrheagenic Escherichia coli and Uropathogenic E. coli Using Multiplex Polymerase Chain Reaction, Jundishapur J Microbiol. 2016 ; 9(2):e28331. doi: 10.5812/jjm.28331.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Russo TA, Stapleton A, Wenderoth S, Hooton TM, Stamm WE. Chromosomal restriction fragment length polymorphism analysis of Escherichia coli strains causing recurrent urinary tract infections in young women. J Infect Dis. 1995; 172(2): 440-5[PubMed]
  • 2. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004; 2(2): 123-40[DOI][PubMed]
  • 3. Mokracka J, Koczura R, Jablonska L, Kaznowski A. Phylogenetic groups, virulence genes and quinolone resistance of integron-bearing Escherichia coli strains isolated from a wastewater treatment plant. Antonie Van Leeuwenhoek. 2011; 99(4): 817-24[DOI][PubMed]
  • 4. Johnson TJ, Nolan LK. Pathogenomics of the virulence plasmids of Escherichia coli. Microbiol Mol Biol Rev. 2009; 73(4): 750-74[DOI][PubMed]
  • 5. Momtaz H, Dehkordi FS, Rahimi E, Asgarifar A. Detection of Escherichia coli, Salmonella species, and Vibrio cholerae in tap water and bottled drinking water in Isfahan, Iran. BMC Public Health. 2013; 13: 556[DOI][PubMed]
  • 6. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science. 2005; 308(5728): 1635-8[DOI][PubMed]
  • 7. Connolly A, Thorp JM. Urinary tract infections in pregnancy. Urol Clin North Am. 1999; 26(4): 779-87[PubMed]
  • 8. McDermott S, Daguise V, Mann H, Szwejbka L, Callaghan W. Perinatal risk for mortality and mental retardation associated with maternal urinary-tract infections. J Fam Pract. 2001; 50(5): 433-7[PubMed]
  • 9. Olsen BE, Hinderaker SG, Lie RT, Gasheka P, Baerheim A, Bergsjo P, et al. The diagnosis of urinary tract infections among pregnant women in rural Tanzania; prevalences and correspondence between different diagnostic methods. Acta Obstet Gynecol Scand. 2000; 79(9): 729-36[PubMed]
  • 10. Stapleton A. A new candidate vaccine for Escherichia coli pyelonephritis. J Urol. 2004; 171(4): 1686-7[PubMed]
  • 11. Dormanesh B, Safarpoor Dehkordi F, Hosseini S, Momtaz H, Mirnejad R, Hoseini MJ, et al. Virulence factors and o-serogroups profiles of uropathogenic Escherichia coli isolated from Iranian pediatric patients. Iran Red Crescent Med J. 2014; 16(2)[DOI][PubMed]
  • 12. Heidary M, Momtaz H, Madani M. Characterization of Diarrheagenic Antimicrobial Resistant Escherichia coli Isolated From Pediatric Patients in Tehran, Iran. Iran Red Crescent Med J. 2014; 16(4)[DOI][PubMed]
  • 13. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1): 142-201[PubMed]
  • 14. Qadri F, Svennerholm AM, Faruque AS, Sack RB. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005; 18(3): 465-83[DOI][PubMed]
  • 15. Avendano P, Matson DO, Long J, Whitney S, Matson CC, Pickering LK. Costs associated with office visits for diarrhea in infants and toddlers. Pediatr Infect Dis J. 1993; 12(11): 897-902[PubMed]
  • 16. Kosek M, Bern C, Guerrant RL. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ. 2003; 81(3): 197-204[PubMed]
  • 17. Mansouri S, Shareifi S. Antimicrobial resistance pattern of Escherichia coli causing urinary tract infections, and that of human fecal flora, in the southeast of Iran. Microb Drug Resist. 2002; 8(2): 123-8[DOI][PubMed]
  • 18. Enayat K, Fariborz S, Heiman S, Mehdi SDM. Frequency, antimicrobial susceptibility and plasmid profiles of Escherichia coli pathotypes obtained from children with acute diarrhea. Jundishapur J Microbiol. 2011; 4(1): 23-8
  • 19. Herzer PJ, Inouye S, Inouye M, Whittam TS. Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. J Bacteriol. 1990; 172(11): 6175-81[PubMed]
  • 20. Selander RK, Caugant D, Whittam T. Genetic structure and variation in natural populations of Escherichia coli. Escherichia coli and Salmonella typhimurium: cellular and molecular biology. 1987;
  • 21. Rajakumar K, Bulach D, Davies J, Ambrose L, Sasakawa C, Adler B. Identification of a chromosomal Shigella flexneri multi-antibiotic resistance locus which shares sequence and organizational similarity with the resistance region of the plasmid NR1. Plasmid. 1997; 37(3): 159-68[DOI][PubMed]
  • 22. Adzitey F, Huda N. Listeria monocytogenes in foods: incidences and possible control measures. Afr J Microbiol Res. 2010; 4: 2848-55
  • 23. Adzitey F, Huda N, Gulam R. Comparison of media for the isolation of Salmonella (XLD and Rambach) and Listeria species (ALOA and Palcam) in naturally contaminated duck samples. Internet J Food Saf. 2011; 13: 20-5
  • 24. Adzitey F, Huda N, Ali GRR. Molecular techniques for detecting and typing of bacteria, advantages and application to foodborne pathogens isolated from ducks. 3 Biotech. 2013; 3(2): 97-107[DOI]
  • 25. Magistrado PA, Garcia MM, Raymundo AK. Isolation and polymerase chain reaction-based detection of Campylobacter jejuni and Campylobacter coli from poultry in the Philippines. Int J Food Microbiol. 2001; 70(1-2): 197-206[PubMed]
  • 26. Keramas G, Bang DD, Lund M, Madsen M, Bunkenborg H, Telleman P, et al. Use of culture, PCR analysis, and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken feces. J Clin Microbiol. 2004; 42(9): 3985-91[DOI][PubMed]
  • 27. Rappelli P, Maddau G, Mannu F, Colombo MM, Fiori PL, Cappuccinelli P. Development of a set of multiplex PCR assays for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. New Microbiol. 2001; 24(1): 77-83[PubMed]
  • 28. Brandal LT, Lindstedt BA, Aas L, Stavnes TL, Lassen J, Kapperud G. Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp. J Microbiol Methods. 2007; 68(2): 331-41[DOI][PubMed]
  • 29. Albert MJ, Faruque SM, Faruque AS, Neogi PK, Ansaruzzaman M, Bhuiyan NA, et al. Controlled study of Escherichia coli diarrheal infections in Bangladeshi children. J Clin Microbiol. 1995; 33(4): 973-7[PubMed]
  • 30. Shehabi AA, Bulos NK, Hajjaj KG. Characterization of diarrhoeagenic Escherichia coli isolates in Jordanian children. Scand J Infect Dis. 2003; 35(6-7): 368-71[PubMed]
  • 31. Nader MI, Aziz IH, Rehman TA. Detection of LT and ST Toxin Genes for E. coli Isolated From UTI. Int J Biol Pharmaceut Res. 2013; 4(12): 1057-60
  • 32. Bonacorsi S, Houdouin V, Mariani-Kurkdjian P, Mahjoub-Messai F, Bingen E. Comparative prevalence of virulence factors in Escherichia coli causing urinary tract infection in male infants with and without bacteremia. J Clin Microbiol. 2006; 44(3): 1156-8[DOI][PubMed]
  • 33. Tornieporth NG, John J, Salgado K, de Jesus P, Latham E, Melo MC, et al. Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR. J Clin Microbiol. 1995; 33(5): 1371-4[PubMed]
  • 34. Al-Gallas N, Bahri O, Bouratbeen A, Ben Haasen A, Ben Aissa R. Etiology of acute diarrhea in children and adults in Tunis, Tunisia, with emphasis on diarrheagenic Escherichia coli: prevalence, phenotyping, and molecular epidemiology. Am J Trop Med Hyg. 2007; 77(3): 571-82[PubMed]
  • 35. Kimata K, Shima T, Shimizu M, Tanaka D, Isobe J, Gyobu Y, et al. Rapid categorization of pathogenic Escherichia coli by multiplex PCR. Microbiol Immunol. 2005; 49(6): 485-92[PubMed]
  • 36. Shaheen HI, Khalil SB, Rao MR, Abu Elyazeed R, Wierzba TF, Peruski LJ, et al. Phenotypic profiles of enterotoxigenic Escherichia coli associated with early childhood diarrhea in rural Egypt. J Clin Microbiol. 2004; 42(12): 5588-95[DOI][PubMed]
  • 37. Flores J, Okhuysen PC. Enteroaggregative Escherichia coli infection. Curr Opin Gastroenterol. 2009; 25(1): 8-11[DOI][PubMed]
  • 38. Keskimaki M, Eklund M, Pesonen H, Heiskanen T, Siitonen A. EPEC, EAEC and STEC in stool specimens: prevalence and molecular epidemiology of isolates. Diagn Microbiol Infect Dis. 2001; 40(4): 151-6[PubMed]
  • 39. Pawlowski SW, Warren CA, Guerrant R. Diagnosis and treatment of acute or persistent diarrhea. Gastroenterology. 2009; 136(6): 1874-86[DOI][PubMed]
  • 40. Sanders JW, Putnam SD, Gould P, Kolisnyk J, Merced N, Barthel V, et al. Diarrheal illness among deployed U.S. military personnel during Operation Bright Star 2001--Egypt. Diagn Microbiol Infect Dis. 2005; 52(2): 85-90[DOI][PubMed]
  • 41. Steiner TS, Samie A, Guerrant RL. Infectious diarrhea: new pathogens and new challenges in developed and developing areas. Clin Infect Dis. 2006; 43(4): 408-10[DOI][PubMed]
  • 42. Donnenberg MS, Whittam TS. Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. J Clin Invest. 2001; 107(5): 539-48[DOI][PubMed]
  • 43. Nataro JP, Mai V, Johnson J, Blackwelder WC, Heimer R, Tirrell S, et al. Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut. Clin Infect Dis. 2006; 43(4): 402-7[DOI][PubMed]
  • 44. Vernacchio L, Vezina RM, Mitchell AA, Lesko SM, Plaut AG, Acheson DW. Diarrhea in American infants and young children in the community setting: incidence, clinical presentation and microbiology. Pediatr Infect Dis J. 2006; 25(1): 2-7[PubMed]
  • 45. Bueris V, Sircili MP, Taddei CR, dos Santos MF, Franzolin MR, Martinez MB, et al. Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz. 2007; 102(7): 839-44[PubMed]
  • 46. Franzolin MR, Alves RC, Keller R, Gomes TA, Beutin L, Barreto ML, et al. Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz. 2005; 100(4): 359-63[DOI][PubMed]
  • 47. Moyo SJ, Maselle SY, Matee MI, Langeland N, Mylvaganam H. Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania. BMC Infect Dis. 2007; 7: 92[DOI][PubMed]
  • 48. Boyd EF, Hartl DL. Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution. J Bacteriol. 1998; 180(5): 1159-65[PubMed]
  • 49. Lecointre G, Rachdi L, Darlu P, Denamur E. Escherichia coli molecular phylogeny using the incongruence length difference test. Mol Biol Evol. 1998; 15(12): 1685-95[PubMed]
  • 50. Toval F, Kohler CD, Vogel U, Wagenlehner F, Mellmann A, Fruth A, et al. Characterization of Escherichia coli isolates from hospital inpatients or outpatients with urinary tract infection. J Clin Microbiol. 2014; 52(2): 407-18[DOI][PubMed]
  • 51. Hosseini A, Azarnezhad A, Fayazi M, Salmani H. Distribution and Comparison of Antibiotic resistance Pathogenicity Islands among UPEC and DEC. Int J Adv Sci eng Technol. 2015; 3(4): 17-21
  • 52. Johnson JR, Stell AL, Delavari P, Murray AC, Kuskowski M, Gaastra W. Phylogenetic and pathotypic similarities between Escherichia coli isolates from urinary tract infections in dogs and extraintestinal infections in humans. J Infect Dis. 2001; 183(6): 897-906[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments