Jundishapur Journal of Microbiology

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Staphylococcus aureus Isolates Carrying Panton-Valentine Leucocidin Genes: Their Frequency, Antimicrobial Patterns, and Association With Infectious Disease in Shahrekord City, Southwest Iran

Laleh Shariati 1 , Majid Validi 2 , Ali Mohammad Hasheminia 3 , Reza Ghasemikhah 4 , Fariborz Kianpour 5 , Ali Karimi 6 , Mohammad Reza Nafisi 6 and Mohammad Amin Tabatabaiefar 7 , 8 , *
Authors Information
1 Department of Molecular Medicine, Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
2 Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Nursing, School of Nursing and Midwifery, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
4 Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran
5 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
6 Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
7 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
8 Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 01, 2016, 9 (1); e28291
  • Published Online: January 2, 2016
  • Article Type: Research Article
  • Received: March 3, 2015
  • Revised: August 28, 2015
  • Accepted: September 26, 2015
  • DOI: 10.5812/jjm.28291

To Cite: Shariati L, Validi M, Hasheminia A M, Ghasemikhah R, Kianpour F, et al. Staphylococcus aureus Isolates Carrying Panton-Valentine Leucocidin Genes: Their Frequency, Antimicrobial Patterns, and Association With Infectious Disease in Shahrekord City, Southwest Iran, Jundishapur J Microbiol. 2016 ; 9(1):e28291. doi: 10.5812/jjm.28291.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Erami M, Soltani B, Taghavi Ardakani A, Moravveji A, Haji Rezaei M, Soltani S, et al. Nasal Carriage and Resistance Pattern of Multidrug Resistant Staphylococcus aureus Among Healthy Children in Kashan, Iran. Iran Red Crescent Med J. 2014; 16(9)[DOI][PubMed]
  • 2. Ghadiri K, Ebrahimi E, Akramipour R, Rezaei M, Khazaei S, Afsharin MA, Afsharian MI, Sedighi I. Nasal carriage rate of community-and hospital-acquired methicillin-resistant staphylococcus aureus in children, Kermanshah, Iran. Arch Clin Infect Dis. 2012; 6(3): 117-20
  • 3. Holmes A, Ganner M, McGuane S, Pitt TL, Cookson BD, Kearns AM. Staphylococcus aureus isolates carrying Panton-Valentine leucocidin genes in England and Wales: frequency, characterization, and association with clinical disease. J Clin Microbiol. 2005; 43(5): 2384-90[DOI][PubMed]
  • 4. Korn GP, Martino MD, Mimica IM, Mimica LJ, Chiavone PA, Musolino LR. High frequency of colonization and absence of identifiable risk factors for methicillin-resistant Staphylococcus aureus (MRSA)in intensive care units in Brazil. Braz J Infect Dis. 2001; 5(1): 1-7[PubMed]
  • 5. Enright MC, Robinson DA, Randle G, Feil EJ, Grundmann H, Spratt BG. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci U S A. 2002; 99(11): 7687-92[DOI][PubMed]
  • 6. Vandenesch F, Naimi T, Enright MC, Lina G, Nimmo GR, Heffernan H, et al. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis. 2003; 9(8): 978-84[DOI][PubMed]
  • 7. Teng CS, Lo WT, Wang SR, Tseng MH, Chu ML, Wang CC. The role of antimicrobial therapy for treatment of uncomplicated skin and soft tissue infections from community-acquired methicillin-resistant Staphylococcus aureus in children. J Microbiol Immunol Infect. 2009; 42(4): 324-8[PubMed]
  • 8. Boyle-Vavra S, Daum RS. Community-acquired methicillin-resistant Staphylococcus aureus: the role of Panton-Valentine leukocidin. Lab Invest. 2007; 87(1): 3-9[DOI][PubMed]
  • 9. Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C, et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2001; 45(5): 1323-36[DOI][PubMed]
  • 10. Liassine N, Auckenthaler R, Descombes MC, Bes M, Vandenesch F, Etienne J. Community-acquired methicillin-resistant Staphylococcus aureus isolated in Switzerland contains the Panton-Valentine leukocidin or exfoliative toxin genes. J Clin Microbiol. 2004; 42(2): 825-8[PubMed]
  • 11. Magira EE, Zervakis D, Routsi C, Kontogiorgi M, Roussos C, Nanas S, et al. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: a lethal cause of pneumonia in an adult immunocompetent patient. Scand J Infect Dis. 2007; 39(5): 466-9[DOI][PubMed]
  • 12. Vandenesch F, Lina G, Gillet Y, Etienne J, Cremieux AC. [The end of the controversy: Panton Valentine is the culprit]. Med Sci (Paris). 2009; 25(11): 984-6[DOI][PubMed]
  • 13. Meyer F, Girardot R, Piemont Y, Prevost G, Colin DA. Analysis of the specificity of Panton-Valentine leucocidin and gamma-hemolysin F component binding. Infect Immun. 2009; 77(1): 266-73[DOI][PubMed]
  • 14. McDonald RR, Antonishyn NA, Hansen T, Snook LA, Nagle E, Mulvey MR, et al. Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005; 43(12): 6147-9[DOI][PubMed]
  • 15. Mulvey MR, MacDougall L, Cholin B, Horsman G, Fidyk M, Woods S, et al. Community-associated methicillin-resistant Staphylococcus aureus, Canada. Emerg Infect Dis. 2005; 11(6): 844-50[DOI][PubMed]
  • 16. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999; 29(5): 1128-32[DOI][PubMed]
  • 17. Johnsson D, Molling P, Stralin K, Soderquist B. Detection of Panton-Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects. Clin Microbiol Infect. 2004; 10(10): 884-9[DOI][PubMed]
  • 18. Nakagawa S, Taneike I, Mimura D, Iwakura N, Nakayama T, Emura T, et al. Gene sequences and specific detection for Panton-Valentine leukocidin. Biochem Biophys Res Commun. 2005; 328(4): 995-1002[DOI][PubMed]
  • 19. Cheung AS, Aboltins CA, Daffy JR, Stanley PA. Necrotising pneumonia due to Panton-Valentine leukocidin-positive methicillin-sensitive Staphylococcus aureus. Med J Aust. 2008; 188(6): 373[PubMed]
  • 20. Kale-Pradhan P, Johnson LB. Treatment and recurrence management of staphylococcal infections: community-acquired MRSA. Expert Rev Anti Infect Ther. 2008; 6(6): 909-15[DOI][PubMed]
  • 21. Lee MC, Rios AM, Aten MF, Mejias A, Cavuoti D, McCracken GJ, et al. Management and outcome of children with skin and soft tissue abscesses caused by community-acquired methicillin-resistant Staphylococcus aureus. Pediatr Infect Dis J. 2004; 23(2): 123-7[DOI][PubMed]
  • 22. Gaillot O, Wetsch M, Fortineau N, Berche P. Evaluation of CHROMagar Staph. aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens. J Clin Microbiol. 2000; 38(4): 1587-91[PubMed]
  • 23. Shariati L, Validi M, Tabatabaiefar MA, Karimi A, Nafisi MR. Comparison of real-time PCR with disk diffusion, agar screen and E-test methods for detection of methicillin-resistant Staphylococcus aureus. Curr Microbiol. 2010; 61(6): 520-4[DOI][PubMed]
  • 24. Performance Standard for antimicrobial disk susceptibility tests. wayne; CLSI. 2006;
  • 25. Hsu DI, Hidayat LK, Quist R, Hindler J, Karlsson A, Yusof A, et al. Comparison of method-specific vancomycin minimum inhibitory concentration values and their predictability for treatment outcome of meticillin-resistant Staphylococcus aureus (MRSA) infections. Int J Antimicrob Agents. 2008; 32(5): 378-85[DOI][PubMed]
  • 26. Dumitrescu O, Badiou C, Bes M, Reverdy ME, Vandenesch F, Etienne J, et al. Effect of antibiotics, alone and in combination, on Panton-Valentine leukocidin production by a Staphylococcus aureus reference strain. Clin Microbiol Infect. 2008; 14(4): 384-8[DOI][PubMed]
  • 27. Krziwanek K, Luger C, Sammer B, Stumvoll S, Stammler M, Metz-Gercek S, et al. PVL-positive MRSA in Austria. Eur J Clin Microbiol Infect Dis. 2007; 26(12): 931-5[DOI][PubMed]
  • 28. Narita S, Kaneko J, Chiba J, Piemont Y, Jarraud S, Etienne J, et al. Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT. Gene. 2001; 268(1-2): 195-206[PubMed]
  • 29. Antri K, Rouzic N, Boubekri I, Dauwalder O, Beloufa A, Ziane H, et al. [High prevalence of community- and hospital-acquired infections of methicillin-resistant Staphylococcus aureus containing Panton-Valentine leukocidin gene in Algiers]. Pathol Biol (Paris). 2010; 58(2)-20[DOI][PubMed]
  • 30. Afroz S, Kobayashi N, Nagashima S, Alam MM, Hossain AB, Rahman MA, et al. Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh. Jpn J Infect Dis. 2008; 61(5): 393-6[PubMed]
  • 31. Chini V, Petinaki E, Foka A, Paratiras S, Dimitracopoulos G, Spiliopoulou I. Spread of Staphylococcus aureus clinical isolates carrying Panton-Valentine leukocidin genes during a 3-year period in Greece. Clin Microbiol Infect. 2006; 12(1): 29-34[DOI][PubMed]
  • 32. Monecke S, Muller E, Dorneanu OS, Vremera T, Ehricht R. Molecular typing of MRSA and of clinical Staphylococcus aureus isolates from Iasi, Romania. PLoS One. 2014; 9(5)[DOI][PubMed]
  • 33. Shrestha B, Singh W, Raj VS, Pokhrel BM, Mohapatra TM. High prevalence of Panton-Valentine leukocidin (PVL) genes in nosocomial-acquired Staphylococcus aureus isolated from tertiary care hospitals in Nepal. Biomed Res Int. 2014; 2014: 790350[DOI][PubMed]
  • 34. Khosravi AD, Hoveizavi H, Farshadzadeh Z. The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani Hospital, Ahvaz, Iran. Burns. 2012; 38(2): 247-51[DOI][PubMed]
  • 35. Hoseini Alfatemi SM, Motamedifar M, Hadi N, Sedigh Ebrahim Saraie H. Analysis of Virulence Genes Among Methicillin Resistant Staphylococcus aureus (MRSA) Strains. Jundishapur J Microbiol. 2014; 7(6)[DOI][PubMed]
  • 36. Havaei S, Moghadam SO, Pourmand M, Faghri J. Prevalence of Genes Encoding Bi-Component Leukocidins among Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Iran J Public Health. 2010; 39(1): 8-14[PubMed]
  • 37. Kilic A, Guclu AU, Senses Z, Bedir O, Aydogan H, Basustaoglu AC. Staphylococcal cassette chromosome mec (SCCmec) characterization and panton-valentine leukocidin gene occurrence for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006. Antonie Van Leeuwenhoek. 2008; 94(4): 607-14[DOI][PubMed]
  • 38. Bocchini CE, Hulten KG, Mason EO, Gonzalez BE, Hammerman WA, Kaplan SL. Panton-Valentine leukocidin genes are associated with enhanced inflammatory response and local disease in acute hematogenous Staphylococcus aureus osteomyelitis in children. Pediatrics. 2006; 117(2): 433-40[DOI][PubMed]
  • 39. Gubbay JB, Gosbell IB, Barbagiannakos T, Vickery AM, Mercer JL, Watson M. Clinical features, epidemiology, antimicrobial resistance, and exotoxin genes (including that of Panton-Valentine leukocidin) of gentamicin-susceptible methicillin-resistant Staphylococcus aureus (GS-MRSA) isolated at a paediatric teaching hospital in New South Wales, Australia. Pathology. 2008; 40(1): 64-71[DOI][PubMed]
  • 40. Mariem BJ, Ito T, Zhang M, Jin J, Li S, Ilhem BB, et al. Molecular characterization of methicillin-resistant Panton-valentine leukocidin positive staphylococcus aureus clones disseminating in Tunisian hospitals and in the community. BMC Microbiol. 2013; 13: 2[DOI][PubMed]
  • 41. Morgan MS. Diagnosis and treatment of Panton-Valentine leukocidin (PVL)-associated staphylococcal pneumonia. Int J Antimicrob Agents. 2007; 30(4): 289-96[DOI][PubMed]
  • 42. Prevost G, Couppie P, Prevost P, Gayet S, Petiau P, Cribier B, et al. Epidemiological data on Staphylococcus aureus strains producing synergohymenotropic toxins. J Med Microbiol. 1995; 42(4): 237-45[DOI][PubMed]
  • 43. Couppie P, Cribier B, Prevost G. Leukocidin from Staphylococcus aureus and cutaneous infections: an epidemiologic study. Arch Dermatol. 1994; 130(9): 1208-9[PubMed]
  • 44. Francis JS, Doherty MC, Lopatin U, Johnston CP, Sinha G, Ross T, et al. Severe community-onset pneumonia in healthy adults caused by methicillin-resistant Staphylococcus aureus carrying the Panton-Valentine leukocidin genes. Clin Infect Dis. 2005; 40(1): 100-7[DOI][PubMed]
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