Jundishapur Journal of Microbiology

Published by: Kowsar

Expression and Purification of the Uropathogenic Escherichia coli PapG Protein and its Surface Absorption on Lactobacillus reuteri: Implications for Surface Display System Vaccines

Fatemeh Ashrafi 1 , Jalil Fallah Mehrabadi 2 , * , Seyed Davar Siadat 3 and Mohammad Reza Aghasadeghi 4
Authors Information
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IR Iran
2 Department of Biology, School of Basic Science, University of Qom, Qom, IR Iran
3 Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran
4 Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: September 01, 2015, 8 (9); e25595
  • Published Online: September 8, 2015
  • Article Type: Research Article
  • Received: November 25, 2014
  • Revised: December 23, 2014
  • Accepted: January 24, 2015
  • DOI: 10.5812/jjm.25595

To Cite: Ashrafi F, Fallah Mehrabadi J, Siadat S D, Aghasadeghi M R. Expression and Purification of the Uropathogenic Escherichia coli PapG Protein and its Surface Absorption on Lactobacillus reuteri: Implications for Surface Display System Vaccines, Jundishapur J Microbiol. 2015 ; 8(9):e25595. doi: 10.5812/jjm.25595.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Johnson JR, Owens K, Gajewski A, Kuskowski MA. Bacterial characteristics in relation to clinical source of Escherichia coli isolates from women with acute cystitis or pyelonephritis and uninfected women. J Clin Microbiol. 2005; 43(12): 6064-72[DOI][PubMed]
  • 2. Snyder JA, Lloyd AL, Lockatell CV, Johnson DE, Mobley HL. Role of phase variation of type 1 fimbriae in a uropathogenic Escherichia coli cystitis isolate during urinary tract infection. Infect Immun. 2006; 74(2): 1387-93[DOI][PubMed]
  • 3. Hooton TM. Recurrent urinary tract infection in women. Int J Antimicrob Agents. 2001; 17(4): 259-68[DOI]
  • 4. Dodson KW, Pinkner JS, Rose T, Magnusson G, Hultgren SJ, Waksman G. Structural basis of the interaction of the pyelonephritic E. coli adhesin to its human kidney receptor. Cell. 2001; 105(6): 733-43[PubMed]
  • 5. Svanborg C, Godaly G. Bacterial Virulence in Urinary Tract Infection. Infect Dis Clin North Am. 1997; 11(3): 513-29[DOI]
  • 6. Kausar Y, Chunchanur SK, Nadagir SD, Halesh LH, Chandrasekhar MR. Virulence factors, serotypes and antimicrobial suspectibility pattern of Escherichia coli in urinary tract infections. Al Ameen J Med Sci. 2009; 2(1): 47-51
  • 7. Karr JF, Nowicki BJ, Truong LD, Hull RA, Moulds JJ, Hull SI. pap-2-encoded fimbriae adhere to the P blood group-related glycosphingolipid stage-specific embryonic antigen 4 in the human kidney. Infect Immun. 1990; 58(12): 4055-62[PubMed]
  • 8. Blanco M, Blanco JE, Alonso MP, Blanco J. Virulence factors and O groups of Escherichia coli isolates from patients with acute pyelonephritis, cystitis and asymptomatic bacteriuria. Eur J Epidemiol. 1996; 12(2): 191-8[PubMed]
  • 9. Lane MC, Mobley HL. Role of P-fimbrial-mediated adherence in pyelonephritis and persistence of uropathogenic Escherichia coli (UPEC) in the mammalian kidney. Kidney Int. 2007; 72(1): 19-25[DOI][PubMed]
  • 10. Johnson JR, Russo TA, Brown JJ, Stapleton A. papG alleles of Escherichia coli strains causing first-episode or recurrent acute cystitis in adult women. J Infect Dis. 1998; 177(1): 97-101[PubMed]
  • 11. Johanson I, Lindstedt R, Svanborg C. Roles of the pap- and prs-encoded adhesins in Escherichia coli adherence to human uroepithelial cells. Infect Immun. 1992; 60(8): 3416-22[PubMed]
  • 12. Al-Mayahie SM. Vaginal colonization by papG allele II+ Escherichia coli isolates from pregnant and nonpregnant women as predisposing factor to pyelonephritis. Infect Dis Obstet Gynecol. 2013; 2013: 860402[DOI][PubMed]
  • 13. Liu MA. DNA vaccines: a review. J Intern Med. 2003; 253(4): 402-10[PubMed]
  • 14. Matar C, Valdez JC, Medina M, Rachid M, Perdigon G. Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant. J Dairy Res. 2001; 68(4): 601-9[PubMed]
  • 15. Jian-Kui L, Xi-Lin H, Chun-Hua W, Li-Yun Y, Xiao-Jie H, Gui-Hua W, et al. Induction of Immune Responses in Mice after Oral Immunization with Recombinant Lactobacillus casei Strains Expressing EnterotoxigenicEscherichia coli F41 Fimbrial Protein. Appl Environ Microbiol. 2009; 75(13): 4491_4497
  • 16. Lee JS, Poo H, Han DP, Hong SP, Kim K, Cho MW, et al. Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice. J Virol. 2006; 80(8): 4079-87[DOI][PubMed]
  • 17. Wu CM, Chung TC. Mice protected by oral immunization with Lactobacillus reuteri secreting fusion protein of Escherichia coli enterotoxin subunit protein. FEMS Immunol Med Microbiol. 2007; 50(3): 354-65[DOI][PubMed]
  • 18. Roberts JA, Kaack MB, Baskin G, Chapman MR, Hunstad DA, Pinkner JS, et al. Antibody responses and protection from pyelonephritis following vaccination with purified Escherichia coli PapDG protein. J Urol. 2004; 171(4): 1682-5[DOI][PubMed]
  • 19. Leenhouts K, Buist G, Kok J. Anchoring of proteins to lactic acid bacteria. Antonie Van Leeuwenhoek. 1999; 76(1-4): 367-76[PubMed]
  • 20. Singaravelu M, Selvan A, Anishetty S. Molecular dynamics simulations of lectin domain of FimH and immunoinformatics for the design of potential vaccine candidates. Comput Biol Chem. 2014; 52: 18-24[DOI][PubMed]
  • 21. Geerlings SE, Beerepoot MA, Prins JM. Prevention of recurrent urinary tract infections in women: antimicrobial and nonantimicrobial strategies. Infect Dis Clin North Am. 2014; 28(1): 135-47[DOI][PubMed]
  • 22. Moriel DG, Schembri MA. Vaccination approaches for the prevention of urinary tract infection. Curr Pharm Biotechnol. 2013; 14(11): 967-74[PubMed]
  • 23. Lakritz JR, Poutahidis T, Levkovich T, Varian BJ, Ibrahim YM, Chatzigiagkos A, et al. Beneficial bacteria stimulate host immune cells to counteract dietary and genetic predisposition to mammary cancer in mice. Int J Cancer. 2014; 135(3): 529-40[DOI][PubMed]
  • 24. Forsberg A, Abrahamsson TR, Bjorksten B, Jenmalm MC. Pre- and post-natal Lactobacillus reuteri supplementation decreases allergen responsiveness in infancy. Clin Exp Allergy. 2013; 43(4): 434-42[DOI][PubMed]
  • 25. Mechoud MA, Mateos MV, de Valdez GF, Villena J, Salvador GA, Rodriguez AV. Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts. Int Immunopharmacol. 2012; 14(4): 446-53[DOI][PubMed]
  • 26. Eslami G, Karimiravesh R, Taheri S, Azargashb E. Inhibitory Effect of Lactobacillus reuterion Some Pathogenic Bacteria Isolated From Women With Bacterial Vaginosis. Avicenna J Clin Microb Infect. 2014; 1(2)
  • 27. Raha AR, Varma NR, Yusoff K, Ross E, Foo HL. Cell surface display system for Lactococcus lactis: a novel development for oral vaccine. Appl Microbiol Biotechnol. 2005; 68(1): 75-81[DOI][PubMed]
  • 28. Song D, Gu Q. Surface expression of Helicobacter pylori urease subunit B gene E fragment on Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A. Biotechnol Lett. 2009; 31(7): 985-9[DOI][PubMed]
  • 29. Qin J, Wang X, Kong J, Ma C, Xu P. Construction of a food-grade cell surface display system for Lactobacillus casei. Microbiol Res. 2014; 169(9-10): 733-40[DOI][PubMed]
  • 30. Kim TW, Igimi S, Kajikawa A, Kim HY. Display of heterologous proteins on the surface of Lactococcus lactis using the H and W domain of PrtB from Lactobacillus delburueckii subsp. bulgaricus as an anchoring matrix. J Appl Microbiol. 2008; 104(6): 1636-43[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments