Jundishapur Journal of Microbiology

Published by: Kowsar

Crimean-Congo Hemorrhagic Fever Virus Gn Bioinformatic Analysis and Construction of a Recombinant Bacmid in Order to Express Gn by Baculovirus Expression System

Mehdi Rahpeyma 1 , Fatemeh Fotouhi 2 , Manouchehr Makvandi 3 , Ata Ghadiri 4 and Alireza Samarbaf-Zadeh 1 , *
Authors Information
1 Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Influenza Research Laboratory, Department of Virology, Pasteur Institute of Iran, Tehran, IR Iran
3 Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
4 Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2015, 8 (11); e25502
  • Published Online: November 21, 2015
  • Article Type: Research Article
  • Received: December 27, 2014
  • Revised: June 28, 2015
  • Accepted: August 4, 2015
  • DOI: 10.5812/jjm.25502

To Cite: Rahpeyma M, Fotouhi F, Makvandi M, Ghadiri A, Samarbaf-Zadeh A. Crimean-Congo Hemorrhagic Fever Virus Gn Bioinformatic Analysis and Construction of a Recombinant Bacmid in Order to Express Gn by Baculovirus Expression System, Jundishapur J Microbiol. 2015 ; 8(11):e25502. doi: 10.5812/jjm.25502.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Sanchez AJ, Vincent MJ, Nichol ST. Characterization of the glycoproteins of Crimean-Congo hemorrhagic fever virus. J Virol. 2002; 76(14): 7263-75[PubMed]
  • 2. Canakoglu N, Berber E, Ertek M, Yoruk MD, Tonbak S, Bolat Y, et al. Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus. Virol J. 2013; 10: 6[DOI][PubMed]
  • 3. Flick R, Flick K, Feldmann H, Elgh F. Reverse genetics for crimean-congo hemorrhagic fever virus. J Virol. 2003; 77(10): 5997-6006[PubMed]
  • 4. Mardani M, Keshtkar-Jahromi M, Ataie B, Adibi P. Crimean-Congo hemorrhagic fever virus as a nosocomial pathogen in Iran. Am J Trop Med Hyg. 2009; 81(4): 675-8[DOI][PubMed]
  • 5. Bente DA, Alimonti JB, Shieh WJ, Camus G, Stroher U, Zaki S, et al. Pathogenesis and immune response of Crimean-Congo hemorrhagic fever virus in a STAT-1 knockout mouse model. J Virol. 2010; 84(21): 11089-100[DOI][PubMed]
  • 6. Garrison AR, Radoshitzky SR, Kota KP, Pegoraro G, Ruthel G, Kuhn JH, et al. Crimean-Congo hemorrhagic fever virus utilizes a clathrin- and early endosome-dependent entry pathway. Virology. 2013; 444(1-2): 45-54[DOI][PubMed]
  • 7. Izadi S, Naieni KH, Madjdzadeh SR, Nadim A. Crimean-Congo hemorrhagic fever in Sistan and Baluchestan Province of Iran, a case-control study on epidemiological characteristics. Int J Infect Dis. 2004; 8(5): 299-306[DOI][PubMed]
  • 8. Mardani M, Jahromi MK, Naieni KH, Zeinali M. The efficacy of oral ribavirin in the treatment of crimean-congo hemorrhagic fever in Iran. Clin Infect Dis. 2003; 36(12): 1613-8[DOI][PubMed]
  • 9. Sharifi-Mood B, Metanat M, Hashemi-Shahri SM, Mardani M, Hashemi SA, Fayyaz-Jahani F. Crimean-Congo hemorrhagic fever following consumption of uncooked liver: case series study. Iran J Clin Infect Dis. 2011; 6(3): 128-30
  • 10. Rahbari S, Nabian S, Shayan P. Primary report on distribution of tick fauna in Iran. Parasitol Res. 2007; 101 Suppl 2-7[DOI][PubMed]
  • 11. Lak SS, Vatandoost H, Telmadarraiy Z, Mahdi RE, Kia E. Seasonal activity of ticks and their importance in tick-borne infectious diseases in West Azerbaijan, Iran. J Arthropod-Borne Dis. 2008; 2(2): 28-34
  • 12. Nabian S, Rahbari S. Occurrence of soft and hard ticks on ruminants in Zagros mountainous areas of Iran. J Arthropod-Borne Dis. 2008; 2(1): 16-20
  • 13. Carter SD, Surtees R, Walter CT, Ariza A, Bergeron E, Nichol ST, et al. Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein. J Virol. 2012; 86(20): 10914-23[DOI][PubMed]
  • 14. Kato T, Thompson JR, Park EY. Construction of new ligation-independent cloning vectors for the expression and purification of recombinant proteins in silkworms using BmNPV bacmid system. PLoS One. 2013; 8(5)[DOI][PubMed]
  • 15. Roy P, Noad R. Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives. ISRN microbiol. 2012; 2012: 11
  • 16. Abdel-Moneim AS, Giesow K, Keil GM. High-level protein expression following single and dual gene cloning of infectious bronchitis virus N and S genes using baculovirus systems. Viral Immunol. 2014; 27(2): 75-81[DOI][PubMed]
  • 17. Bertolotti-Ciarlet A, Smith J, Strecker K, Paragas J, Altamura LA, McFalls JM, et al. Cellular localization and antigenic characterization of crimean-congo hemorrhagic fever virus glycoproteins. J Virol. 2005; 79(10): 6152-61[DOI][PubMed]
  • 18. Bergeron E, Albarino CG, Khristova ML, Nichol ST. Crimean-Congo hemorrhagic fever virus-encoded ovarian tumor protease activity is dispensable for virus RNA polymerase function. J virology. 2010; 84(1): 216-26[DOI][PubMed]
  • 19. Altamura LA, Bertolotti-Ciarlet A, Teigler J, Paragas J, Schmaljohn CS, Doms RW. Identification of a novel C-terminal cleavage of Crimean-Congo hemorrhagic fever virus PreGN that leads to generation of an NSM protein. J Virol. 2007; 81(12): 6632-42[DOI][PubMed]
  • 20. Sato Y, Takaya A, Yamamoto T. Meta-analytic approach to the accurate prediction of secreted virulence effectors in gram-negative bacteria. BMC Bioinformatics. 2011; 12: 442[DOI][PubMed]
  • 21. McGuffin LJ, Bryson K, Jones DT. The PSIPRED protein structure prediction server. Bioinformatics. 2000; 16(4): 404-5[PubMed]
  • 22. Zhang Y. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics. 2008; 9: 40[DOI][PubMed]
  • 23. Erickson BR, Deyde V, Sanchez AJ, Vincent MJ, Nichol ST. N-linked glycosylation of Gn (but not Gc) is important for Crimean Congo hemorrhagic fever virus glycoprotein localization and transport. Virology. 2007; 361(2): 348-55[DOI][PubMed]
  • 24. Carneiro S, Ferreira EC, Rocha I. Metabolic responses to recombinant bioprocesses in Escherichia coli. J Biotechnol. 2013; 164(3): 396-408[DOI][PubMed]
  • 25. Nallamsetty S, Waugh DS. A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag. Nat Protoc. 2007; 2(2): 383-91[DOI][PubMed]
  • 26. Jarvis DL. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology. 2003; 310(1): 1-7[PubMed]
  • 27. Harrison RL, Jarvis DL. Protein N‐Glycosylation in the Baculovirus–Insect Cell Expression System and Engineering of Insect Cells to Produce “Mammalianized” Recombinant Glycoproteins. Adv virus res. 2006; 68: 159-91[DOI]
  • 28. Ramya R, Mohana Subramanian B, Sivakumar V, Senthilkumar RL, Sambasiva Rao KR, Srinivasan VA. Expression and solubilization of insect cell-based rabies virus glycoprotein and assessment of its immunogenicity and protective efficacy in mice. Clin Vaccine Immunol. 2011; 18(10): 1673-9[DOI][PubMed]
  • 29. Metz SW, Geertsema C, Martina BE, Andrade P, Heldens JG, van Oers MM, et al. Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells. Virol J. 2011; 8: 353[DOI][PubMed]
  • 30. Barros MC, Galasso TG, Chaib AJ, Degallier N, Nagata T, Ribeiro BM. Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human sera. Virol J. 2011; 8: 261[DOI][PubMed]
  • 31. Kim S, Chang J. Baculovirus-based Vaccine Displaying Respiratory Syncytial Virus Glycoprotein Induces Protective Immunity against RSV Infection without Vaccine-Enhanced Disease. Immune Netw. 2012; 12(1): 8-17[DOI][PubMed]
  • 32. Zoth SC, Gomez E, Carballeda JM, Carrillo E, Berinstein A. Expression of a secreted version of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: its evaluation as a diagnostic reagent. J Vet Diagn Invest. 2011; 23(3): 519-23[DOI][PubMed]
  • 33. Shi X, Jarvis DL. Protein N-glycosylation in the baculovirus-insect cell system. Curr Drug Targets. 2007; 8(10): 1116-25[PubMed]
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