Jundishapur Journal of Microbiology

Published by: Kowsar

Heterologous Expression of Hepatitis C Virus Core Protein in Oil Seeds of Brassica napus L.

Sara Mohammadzadeh 1 , Farzin Roohvand 2 , Soheila Ajdary 3 , Parastoo Ehsani 1 , * and Ali Hatef Salmanian 4 , *
Authors Information
1 Molecular Biology Department, Pasteur Institute of Iran, Tehran, IR Iran
2 Virology Department, Pasteur Institute of Iran, Tehran, IR Iran
3 Immunology Department, Pasteur Institute of Iran, Tehran, Iran
4 Agricultural Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, IR Iran
Corresponding Authors:
Article information
  • Jundishapur Journal of Microbiology: November 01, 2015, 8 (11); e25462
  • Published Online: November 7, 2015
  • Article Type: Research Article
  • Received: November 19, 2014
  • Revised: February 3, 2015
  • Accepted: February 17, 2015
  • DOI: 10.5812/jjm.25462

To Cite: Mohammadzadeh S, Roohvand F, Ajdary S, Ehsani P, Hatef Salmanian A. Heterologous Expression of Hepatitis C Virus Core Protein in Oil Seeds of Brassica napus L., Jundishapur J Microbiol. 2015 ; 8(11):e25462. doi: 10.5812/jjm.25462.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Hajarizadeh B, Grebely J, Dore GJ. Epidemiology and natural history of HCV infection. Nature Reviews Gastroenterology and Hepatology. 2013; 10(9): 553-62[PubMed]
  • 2. Roohvand F, Kossari N. Advances in hepatitis C virus vaccines, Part one: Advances in basic knowledge for hepatitis C virus vaccine design. Expert Opin Ther Pat. 2011; 21(12): 1811-30[DOI][PubMed]
  • 3. Fauvelle C, Lepiller Q, Felmlee DJ, Fofana I, Habersetzer F, Stoll-Keller F, et al. Hepatitis C virus vaccines--progress and perspectives. Microb Pathog. 2013; 58: 66-72[DOI][PubMed]
  • 4. Halliday J, Klenerman P, Barnes E. Vaccination for hepatitis C virus: closing in on an evasive target. Expert Rev Vaccines. 2011; 10(5): 659-72[DOI][PubMed]
  • 5. Kakimi K, Kuribayashi K, Iwashiro M, Masuda T, Sakai M, Ling W, et al. Hepatitis C virus core region: helper T cell epitopes recognized by BALB/c and C57BL/6 mice. J Gen Virol. 1995; 76 ( Pt 5): 1205-14[DOI][PubMed]
  • 6. Drane D, Maraskovsky E, Gibson R, Mitchell S, Barnden M, Moskwa A, et al. Priming of CD4+ and CD8+ T cell responses using a HCV core ISCOMATRIX vaccine: a phase I study in healthy volunteers. Hum Vaccin. 2009; 5(3): 151-7[PubMed]
  • 7. Habersetzer F, Baumert TF, Stoll-Keller F. GI-5005, a yeast vector vaccine expressing an NS3-core fusion protein for chronic HCV infection. Curr Opin Mol Ther. 2009; 11(4): 456-62[PubMed]
  • 8. Alvarez-Lajonchere L, Shoukry NH, Gra B, Amador-Canizares Y, Helle F, Bedard N, et al. Immunogenicity of CIGB-230, a therapeutic DNA vaccine preparation, in HCV-chronically infected individuals in a Phase I clinical trial. J Viral Hepat. 2009; 16(3): 156-67[DOI][PubMed]
  • 9. Seme K, Poljak M, Babic DZ, Mocilnik T, Vince A. The role of core antigen detection in management of hepatitis C: a critical review. J Clin Virol. 2005; 32(2): 92-101[DOI][PubMed]
  • 10. Zhu W, Chang Y, Wu C, Han Q, Pei R, Lu M, et al. The wild-type hepatitis C virus core inhibits initiation of antigen-specific T- and B-cell immune responses in BALB/c mice. Clin Vaccine Immunol. 2010; 17(7): 1139-47[DOI][PubMed]
  • 11. Mavromara P, Sall A, Kalinina O, Horm V, Budkowska A, H. C. V. Collaborative Team of the International Pasteur Network . The impact of HCV diversity on diagnosis tools for HCV infection. Med Mal Infect. 2005; 35 Suppl 2-4[PubMed]
  • 12. Roohvand F, Aghasadeghi MR, Sadat SM, Budkowska A, Khabiri AR. HCV core protein immunization with Montanide/CpG elicits strong Th1/Th2 and long-lived CTL responses. Biochem Biophys Res Commun. 2007; 354(3): 641-9[DOI][PubMed]
  • 13. Acosta-Rivero N, Duenas-Carrera S, Alvarez-Lajonchere L, Morales-Grillo J. HCV core protein-expressing DNA vaccine induces a strong class I-binding peptide DTH response in mice. Biochem Biophys Res Commun. 2004; 314(3): 781-6[PubMed]
  • 14. Yazdanian M, Memarnejadian A, Mahdavi M, Sadat SM, Motevali F, Vahabpour R, et al. Immunization of Mice by BCG Formulated HCV Core Protein Elicited Higher Th1-Oriented Responses Compared to Pluronic-F127 Copolymer. Hepat Mon. 2013; 13(10)[DOI][PubMed]
  • 15. Roohvand F, Kossari N. Advances in hepatitis C virus vaccines, part two: advances in hepatitis C virus vaccine formulations and modalities. Expert Opin Ther Pat. 2012; 22(4): 391-415[DOI][PubMed]
  • 16. Afzal MS, Zaidi NU, Dubuisson J, Rouille Y. Hepatitis C virus capsid protein and intracellular lipids interplay and its association with hepatic steatosis. Hepat Mon. 2014; 14(8)[DOI][PubMed]
  • 17. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM. Plant-based production of biopharmaceuticals. Curr Opin Plant Biol. 2004; 7(2): 152-8[DOI][PubMed]
  • 18. Memari HR, Ramanan RN, Ariff AB. Comparison of expression systems for the production of human interferon-α2b. Cent Eur J Biol. 2010; 5(4): 446-55[DOI]
  • 19. Guan ZJ, Guo B, Huo YL, Guan ZP, Wei YH. Overview of expression of hepatitis B surface antigen in transgenic plants. Vaccine. 2010; 28(46): 7351-62[DOI][PubMed]
  • 20. Tacket CO, Mason HS, Losonsky G, Estes MK, Levine MM, Arntzen CJ. Human immune responses to a novel norwalk virus vaccine delivered in transgenic potatoes. J Infect Dis. 2000; 182(1): 302-5[DOI][PubMed]
  • 21. Nianiou I, Kalantidis K, Madesis P, Georgopoulou U, Mavromara P, Tsaftaris A. Expression of an HCV core antigen coding gene in tobacco (N. tabacum L.). Prep Biochem Biotechnol. 2008; 38(4): 411-21[DOI][PubMed]
  • 22. Madesis P, Osathanunkul M, Georgopoulou U, Gisby MF, Mudd EA, Nianiou I, et al. A hepatitis C virus core polypeptide expressed in chloroplasts detects anti-core antibodies in infected human sera. J Biotechnol. 2010; 145(4): 377-86[DOI][PubMed]
  • 23. Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R. Molecular farming in plants: host systems and expression technology. Trends Biotechnol. 2003; 21(12): 570-8[DOI][PubMed]
  • 24. Boothe J, Nykiforuk C, Shen Y, Zaplachinski S, Szarka S, Kuhlman P, et al. Seed-based expression systems for plant molecular farming. Plant Biotechnol J. 2010; 8(5): 588-606[DOI][PubMed]
  • 25. Lau OS, Sun SS. Plant seeds as bioreactors for recombinant protein production. Biotechnol Adv. 2009; 27(6): 1015-22[DOI][PubMed]
  • 26. Moravec T, Schmidt MA, Herman EM, Woodford-Thomas T. Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine. Vaccine. 2007; 25(9): 1647-57[DOI][PubMed]
  • 27. Kang TJ, Han SC, Yang MS. Expression of the B subunit of E. coli heat-labile enterotoxin in tobacco using a herbicide resistance gene as a selection marker. Plant Cell, Tissue and Organ Culture. 2005; 81(2): 165-74
  • 28. Martinez-Gonzalez L, Rosales-Mendoza S, Soria-Guerra RE, Moreno-Fierros L, Lopez-Revilla R, Korban SS, et al. Oral immunization with a lettuce-derived Escherichia coli heat-labile toxin B subunit induces neutralizing antibodies in mice. Plant Cell, Tissue and Organ Culture (PCTOC). 2011; 107(3): 441-9
  • 29. Nochi T, Takagi H, Yuki Y, Yang L, Masumura T, Mejima M, et al. Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination. Proc Natl Acad Sci U S A. 2007; 104(26): 10986-91[DOI][PubMed]
  • 30. Penney CA, Thomas DR, Deen SS, Walmsley AM. Plant-made vaccines in support of the Millennium Development Goals. Plant Cell Rep. 2011; 30(5): 789-98[DOI][PubMed]
  • 31. Amani J, Mousavi SL, Rafati S, Salmanian AH. Immunogenicity of a plant-derived edible chimeric EspA, Intimin and Tir of Escherichia coli O157:H7 in mice. Plant Sci. 2011; 180(4): 620-7[DOI][PubMed]
  • 32. Deckers H, Rooijen G, Boothe J, Goll J, Moloney M, Schryvers A, et al. Immunogenic formulations comprising oil bodies 2004;
  • 33. Zebarjadi A, Jalali Javaran M, Karimzadeh G, Moeini A, Mousavi A, Salmanian AH. Transformation of rapeseed (Brassica napus L.) plants with sense and antisense constructs of the fatty acid elongase gene. Iran J Biotechnol. 2006; 4(2): 79-87
  • 34. Maillard P, Lavergne JP, Siberil S, Faure G, Roohvand F, Petres S, et al. Fcgamma receptor-like activity of hepatitis C virus core protein. J Biol Chem. 2004; 279(4): 2430-7[DOI][PubMed]
  • 35. Roohvand F, Maillard P, Lavergne JP, Boulant S, Walic M, Andreo U, et al. Initiation of hepatitis C virus infection requires the dynamic microtubule network: role of the viral nucleocapsid protein. J Biol Chem. 2009; 284(20): 13778-91[DOI][PubMed]
  • 36. Mohammadzadeh S, Khabiri A, Roohvand F, Memarnejadian A, Salmanian AH, Ajdary S, et al. Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications. Hepat Mon. 2014; 14(11)[DOI][PubMed]
  • 37. Kozak M. The scanning model for translation: an update. J Cell Biol. 1989; 108(2): 229-41[PubMed]
  • 38. Munro S, Pelham HR. A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987; 48(5): 899-907[PubMed]
  • 39. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. 1989;
  • 40. Hofgen R, Willmitzer L. Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 1988; 16(20): 9877[PubMed]
  • 41. Kahrizi D, Salmanian AH, Afshari A, Moieni A, Mousavi A. Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate. Plant Cell Rep. 2007; 26(1): 95-104[DOI][PubMed]
  • 42. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-54[PubMed]
  • 43. Bagheri K, Javaran MJ, Mahboudi F, Moeini A, Zebarjadi A. Expression of human interferon gamma in Brassica napus seeds. Afr J Biotechnol. 2013; 9(32): 5066-72
  • 44. Krysan PJ, Young JC, Jester PJ, Monson S, Copenhaver G, Preuss D, et al. Characterization of T-DNA insertion sites in Arabidopsis thaliana and the implications for saturation mutagenesis. OMICS. 2002; 6(2): 163-74[DOI][PubMed]
  • 45. Cheon BY, Kim HJ, Oh KH, Bahn SC, Ahn JH, Choi JW, et al. Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis. Transgenic Res. 2004; 13(6): 541-9[PubMed]
  • 46. Stoger E, Schillberg S, Twyman RM, Fischer R, Christou P. Antibody production in transgenic plants. Methods Mol Biol. 2004; 248: 301-18[PubMed]
  • 47. Love AJ, Chapman SN, Matic S, Noris E, Lomonossoff GP, Taliansky M. In planta production of a candidate vaccine against bovine papillomavirus type 1. Planta. 2012; 236(4): 1305-13[DOI][PubMed]
  • 48. Sharp PM, Li WH. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 1987; 15(3): 1281-95[PubMed]
  • 49. Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987; 6(1): 43-8[PubMed]
  • 50. Amani J, Mousavi SL, Rafati S, Salmanian AH. In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications. Theor Biol Med Model. 2009; 6: 28[DOI][PubMed]
  • 51. Lu Z, Lee KJ, Shao Y, Lee JH, So Y, Choo YK, et al. Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants. J Biomed Biotechnol. 2012; 2012: 364240[DOI][PubMed]
  • 52. Torresi J, Johnson D, Wedemeyer H. Progress in the development of preventive and therapeutic vaccines for hepatitis C virus. J Hepatol. 2011; 54(6): 1273-85[DOI][PubMed]
  • 53. Firbas C, Boehm T, Buerger V, Schuller E, Sabarth N, Jilma B, et al. Immunogenicity and safety of different injection routes and schedules of IC41, a Hepatitis C virus (HCV) peptide vaccine. Vaccine. 2010; 28(12): 2397-407[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments