Jundishapur Journal of Microbiology

Published by: Kowsar

Detection, Virulence Gene Assessment and Antibiotic Resistance Pattern of O157 Enterohemorrhagic Escherichia coli in Tabriz, Iran

Mohammad Taghi Akhi 1 , 2 , 3 , 4 , Ali Toloue Ostadgavahi 1 , 2 , 3 , * , Reza Ghotaslou 4 , Mohammad Asgharzadeh 5 , Tahereh Pirzadeh 4 , Vida Sorayaei Sowmesarayi 3 and Mohammad Yousef Memar 4
Authors Information
1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, IR Iran
3 Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, International Branch, Tabriz, IR Iran
4 Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
5 Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2015, 8 (11); e25317
  • Published Online: November 21, 2015
  • Article Type: Research Article
  • Received: November 13, 2014
  • Revised: July 21, 2015
  • Accepted: August 1, 2015
  • DOI: 10.5812/jjm.25317

To Cite: Akhi M T, Ostadgavahi A T, Ghotaslou R, Asgharzadeh M, Pirzadeh T, et al. Detection, Virulence Gene Assessment and Antibiotic Resistance Pattern of O157 Enterohemorrhagic Escherichia coli in Tabriz, Iran, Jundishapur J Microbiol. 2015 ; 8(11):e25317. doi: 10.5812/jjm.25317.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Karmali MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989; 2(1): 15-38[PubMed]
  • 2. Paton JC, Paton AW. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev. 1998; 11(3): 450-79[PubMed]
  • 3. Khan A, Das SC, Ramamurthy T, Sikdar A, Khanam J, Yamasaki S, et al. Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin-producing Escherichia coli isolates from diverse sources in Calcutta, India. J Clin Microbiol. 2002; 40(6): 2009-15[PubMed]
  • 4. Fagan PK, Hornitzky MA, Bettelheim KA, Djordjevic SP. Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl Environ Microbiol. 1999; 65(2): 868-72[PubMed]
  • 5. Kolling GL, Matthews KR. Export of virulence genes and Shiga toxin by membrane vesicles of Escherichia coli O157:H7. Appl Environ Microbiol. 1999; 65(5): 1843-8[PubMed]
  • 6. Leotta GA, Miliwebsky ES, Chinen I, Espinosa EM, Azzopardi K, Tennant SM, et al. Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand. BMC Microbiol. 2008; 8: 46[DOI][PubMed]
  • 7. Paton AW, Paton JC. Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J Clin Microbiol. 2002; 40(1): 271-4[PubMed]
  • 8. March SB, Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986; 23(5): 869-72[PubMed]
  • 9. Paton AW, Paton JC. Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR. J Clin Microbiol. 1999; 37(10): 3362-5[PubMed]
  • 10. Schroeder CM, Zhao C, DebRoy C, Torcolini J, Zhao S, White DG, et al. Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Appl Environ Microbiol. 2002; 68(2): 576-81[PubMed]
  • 11. Pizarro MA, Orozco JH, Degarbo SM, Calderon AE, Nardello AL, Laciar A, et al. Virulence profiles of Shiga Toxin-Producing Escherichia coli and other potentially diarrheagenic E.coli of bovine origin, in Mendoza, Argentina. Braz J Microbiol. 2013; 44(4): 1173-80[DOI][PubMed]
  • 12. Wada A, Kono M, Kawauchi S, Takagi Y, Morikawa T, Funakoshi K. Rapid discrimination of Gram-positive and Gram-negative bacteria in liquid samples by using NaOH-sodium dodecyl sulfate solution and flow cytometry. PLoS One. 2012; 7(10)[DOI][PubMed]
  • 13. Michigan State University. . STEC Center Website. 2013;
  • 14. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. 2014;
  • 15. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, et al. Foodborne illness acquired in the United States--major pathogens. Emerg Infect Dis. 2011; 17(1): 7-15[DOI][PubMed]
  • 16. Salmanzadeh-Ahrabi S, Habibi E, Jaafari F, Zali MR. Molecular epidemiology of Escherichia coli diarrhoea in children in Tehran. Ann Trop Paediatr. 2005; 25(1): 35-9[DOI][PubMed]
  • 17. Aslani MM, Bouzari S. An epidemiological study on Verotoxin-producing Escherichia coli (VTEC) infection among population of northern region of Iran (Mazandaran and Golestan provinces). Eur J Epidemiol. 2003; 18(4): 345-9[PubMed]
  • 18. Mazaheri S, Salmanzadeh Ahrabi S, Aslani MM. Shiga toxin-producing Escherichia coli isolated from lettuce samples in tehran, iran. Jundishapur J Microbiol. 2014; 7(11)[DOI][PubMed]
  • 19. Fard AH, Bokaeian M, Qureishi ME. Frequency of Escherichia coli O157:H7 in children with diarrhoea in Zahedan, Islamic Republic of Iran. East Mediterr Health J. 2008; 14(5): 1022-7[PubMed]
  • 20. Kesava Naidu G, RGN GSM, Shivannavar CT. Detection of Shiga toxin genes (stx1 & stx2) and molecular characterization of shiga-toxigenic escherichia coli isolated from divers sources in Gulbarga region, India. Pharmacophore. 2011; 2(5): 253-65
  • 21. Mellor GE, Besser TE, Davis MA, Beavis B, Jung W, Smith HV, et al. Multilocus genotype analysis of Escherichia coli O157 isolates from Australia and the United States provides evidence of geographic divergence. Appl Environ Microbiol. 2013; 79(16): 5050-8[DOI][PubMed]
  • 22. Vicentei HIG, do AmaralI LA, Cerqueira AMF. Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms. Braz J Microbiol. 2005; 36(3): 217-22[DOI]
  • 23. Farfan MJ, Torres AG. Molecular mechanisms that mediate colonization of Shiga toxin-producing Escherichia coli strains. Infect Immun. 2012; 80(3): 903-13[DOI][PubMed]
  • 24. Tahamtan Y, Namavari M. Prevalence of O157:H7 and non-O157 E. coli in Iranian domestic sheep. Pak J Biol Sci. 2014; 17(1): 104-8[PubMed]
  • 25. Bonyadian M, Momtaz H, Rahimi E, Habibian R, Yazdani A, Zamani M. Identification & characterization of Shiga toxin-producing Escherichia coli isolates from patients with diarrhoea in Iran. Indian J Med Res. 2010; 132: 328-31[PubMed]
  • 26. Jafari F, Hamidian M, Rezadehbashi M, Doyle M, Salmanzadeh-Ahrabi S, Derakhshan F, et al. Prevalence and antimicrobial resistance of diarrheagenic Escherichia coli and Shigella species associated with acute diarrhea in Tehran, Iran. Can J Infect Dis Med Microbiol. 2009; 20(3)-62[PubMed]
  • 27. Hu GZ, Pan YS, Wu H, Hu H, Xu R, Yuan L, et al. Prevalence of tetracycline resistance genes and identification of tet(M) in clinical isolates of Escherichia coli from sick ducks in China. J Med Microbiol. 2013; 62: 851-8[DOI][PubMed]
  • 28. Armstrong GD, Rowe PC, Goodyer P, Orrbine E, Klassen TP, Wells G, et al. A phase I study of chemically synthesized verotoxin (Shiga-like toxin) Pk-trisaccharide receptors attached to chromosorb for preventing hemolytic-uremic syndrome. J Infect Dis. 1995; 171(4): 1042-5[PubMed]
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