Jundishapur Journal of Microbiology

Published by: Kowsar

Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis

Roohollah Fateh 1 , 2 , Farideh Zaini 2 , * , Parivash Kordbacheh 2 , Mehraban Falahati 3 , Sasan Rezaie 2 , Roshanak Daie Ghazvini 2 , Nahid Borhani 4 , Mahin Safara 2 , Azam Fattahi 2 , Ali Kanani 2 , Shirin Farahyar 3 , Manzar Bolhassani 5 and Mansour Heidari 5 , 6
Authors Information
1 Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, IR Iran
2 Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
4 Department of Parasitology, Pasteur Institute of Iran, Tehran, IR Iran
5 Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, IR Iran
6 Experimental Research Center, Tehran University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2015, 8 (11); e25218
  • Published Online: November 21, 2015
  • Article Type: Research Article
  • Received: November 9, 2014
  • Revised: December 29, 2014
  • Accepted: February 10, 2015
  • DOI: 10.5812/jjm.25218

To Cite: Fateh R, Zaini F, Kordbacheh P, Falahati M, Rezaie S, et al. Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis, Jundishapur J Microbiol. 2015 ; 8(11):e25218. doi: 10.5812/jjm.25218.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Dixon DM, McNeil MM, Cohen ML, Gellin BG, La Montagne JR. Fungal infections: a growing threat. Public Health Rep. 1996; 111(3): 226-35[PubMed]
  • 2. Maccallum DM. Hosting infection: experimental models to assay Candida virulence. Int J Microbiol. 2012; 2012: 363764[DOI][PubMed]
  • 3. Miceli MH, Diaz JA, Lee SA. Emerging opportunistic yeast infections. Lancet Infect Dis. 2011; 11(2): 142-51[DOI][PubMed]
  • 4. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007; 20(1): 133-63[DOI][PubMed]
  • 5. Bodey G, Bueltmann B, Duguid W, Gibbs D, Hanak H, Hotchi M, et al. Fungal infections in cancer patients: an international autopsy survey. Eur J Clin Microbiol Infect Dis. 1992; 11(2): 99-109[PubMed]
  • 6. Boon AP, O'Brien D, Adams DH. 10 year review of invasive aspergillosis detected at necropsy. J Clin Pathol. 1991; 44(6): 452-4[PubMed]
  • 7. Denning DW, Stevens DA. Antifungal and surgical treatment of invasive aspergillosis: review of 2,121 published cases. Rev Infect Dis. 1990; 12(6): 1147-201[PubMed]
  • 8. Iwen PC, Reed EC, Armitage JO, Bierman PJ, Kessinger A, Vose JM, et al. Nosocomial invasive aspergillosis in lymphoma patients treated with bone marrow or peripheral stem cell transplants. Infect Control Hosp Epidemiol. 1993; 14(3): 131-9[PubMed]
  • 9. Morrison VA, Haake RJ, Weisdorf DJ. The spectrum of non-Candida fungal infections following bone marrow transplantation. Medicine (Baltimore). 1993; 72(2): 78-89[PubMed]
  • 10. Marichal P, Gorrens J, Coene MC, Le Jeune L, Vanden Bossche H. Origin of differences in susceptibility of Candida krusei to azole antifungal agents. Mycoses. 1995; 38(3-4): 111-7[PubMed]
  • 11. Marichal P, Vanden Bossche H. Mechanisms of resistance to azole antifungals. Acta Biochim Pol. 1995; 42(4): 509-16[PubMed]
  • 12. Samaranayake YH, Samaranayake LP. Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen. J Med Microbiol. 1994; 41(5): 295-310[DOI][PubMed]
  • 13. Francois IE, Aerts AM, Cammue BP, Thevissen K. Currently used antimycotics: spectrum, mode of action and resistance occurrence. Curr Drug Targets. 2005; 6(8): 895-907[PubMed]
  • 14. Sheehan DJ, Hitchcock CA, Sibley CM. Current and emerging azole antifungal agents. Clin Microbiol Rev. 1999; 12(1): 40-79[PubMed]
  • 15. Wu JJ, Pang KR, Huang DB, Tyring SK. Therapy of systemic fungal infections. Dermatol Ther. 2004; 17(6): 532-8[DOI][PubMed]
  • 16. Kontoyiannis DP, Mantadakis E, Samonis G. Systemic mycoses in the immunocompromised host: an update in antifungal therapy. J Hosp Infect. 2003; 53(4): 243-58[PubMed]
  • 17. Nelson DL, Cox MM. Lehninger Principles of Biochemistry. 2004;
  • 18. Regev-Rudzki N, Karniely S, Ben-Haim NN, Pines O. Yeast aconitase in two locations and two metabolic pathways: seeing small amounts is believing. Mol Biol Cell. 2005; 16(9): 4163-71[DOI][PubMed]
  • 19. Tong WH, Rouault TA. Metabolic regulation of citrate and iron by aconitases: role of iron-sulfur cluster biogenesis. Biometals. 2007; 20(3-4): 549-64[DOI][PubMed]
  • 20. Doi M, Homma M, Chindamporn A, Tanaka K. Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically important Candida species. J Gen Microbiol. 1992; 138(10): 2243-51[DOI][PubMed]
  • 21. Taxonomic significance of the coenzyme Q system in yeasts and yeast-like fungi. In: Iwata K (ed) Yeasts and yeast like organisms in medical science. . Tokyo International Special Symposium on Yeasts. : 61-9
  • 22. Schaefer BC. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal Biochem. 1995; 227(2): 255-73[DOI][PubMed]
  • 23. Ohara O, Dorit RL, Gilbert W. One-sided polymerase chain reaction: the amplification of cDNA. Proc Natl Acad Sci U S A. 1989; 86(15): 5673-7[PubMed]
  • 24. Loh EY, Elliott JF, Cwirla S, Lanier LL, Davis MM. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science. 1989; 243(4888): 217-20[PubMed]
  • 25. Shi X, Jarvis DL. A new rapid amplification of cDNA ends method for extremely guanine plus cytosine-rich genes. Anal Biochem. 2006; 356(2): 222-8[DOI][PubMed]
  • 26. Diezmann S, Cox CJ, Schonian G, Vilgalys RJ, Mitchell TG. Phylogeny and evolution of medical species of Candida and related taxa: a multigenic analysis. J Clin Microbiol. 2004; 42(12): 5624-35[DOI][PubMed]
  • 27. Garrett RHGC. Biochemistry. 1999;
  • 28. Emptage MH, Kent TA, Kennedy MC, Beinert H, Munck E. Mossbauer and EPR studies of activated aconitase: development of a localized valence state at a subsite of the [4Fe-4S] cluster on binding of citrate. Proc Natl Acad Sci U S A. 1983; 80(15): 4674-8[PubMed]
  • 29. Kennedy MC, Werst M, Telser J, Emptage MH, Beinert H, Hoffman BM. Mode of substrate carboxyl binding to the [4Fe-4S]+ cluster of reduced aconitase as studied by 17O and 13C electron-nuclear double resonance spectroscopy. Proc Natl Acad Sci U S A. 1987; 84(24): 8854-8[PubMed]
  • 30. Cleland WW, Kreevoy MM. Low-barrier hydrogen bonds and enzymic catalysis. Science. 1994; 264(5167): 1887-90[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments