Jundishapur Journal of Microbiology

Published by: Kowsar

Comparative Effect of Recombinant Shiga Toxin in Induction of Pro- and Anti-Apoptotic Markers and Inflammatory Cytokines in Epithelial and Monocytic Cells

Fatemeh Abedi Jafari 1 , Mana Oloomi 1 , * and Saeid Bouzari 1
Authors Information
1 Molecular Biology Department, Pasteur Institute of Iran, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: February 01, 2016, 9 (2); e24758
  • Published Online: February 28, 2016
  • Article Type: Research Article
  • Received: October 25, 2014
  • Revised: October 10, 2015
  • Accepted: October 26, 2015
  • DOI: 10.5812/jjm.24758

To Cite: Abedi Jafari F, Oloomi M, Bouzari S. Comparative Effect of Recombinant Shiga Toxin in Induction of Pro- and Anti-Apoptotic Markers and Inflammatory Cytokines in Epithelial and Monocytic Cells, Jundishapur J Microbiol. 2016 ; 9(2):e24758. doi: 10.5812/jjm.24758.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Footnotes
References
  • 1. Bloch SK, Felczykowska A, Nejman-Falenczyk B. Escherichia coli O104:H4 outbreak--have we learnt a lesson from it? Acta Biochim Pol. 2012; 59(4): 483-8[PubMed]
  • 2. Canpolat N. Hemolytic uremic syndrome. Turk Pediatri Ars. 2015; 50(2): 73-82[DOI][PubMed]
  • 3. Jacewicz M, Clausen H, Nudelman E, Donohue-Rolfe A, Keusch GT. Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide. J Exp Med. 1986; 163(6): 1391-404[PubMed]
  • 4. Lingwood CA, Khine AA, Arab S. Globotriaosyl ceramide (Gb3) expression in human tumour cells: intracellular trafficking defines a new retrograde transport pathway from the cell surface to the nucleus, which correlates with sensitivity to verotoxin. Acta Biochim Pol. 1998; 45(2): 351-9[PubMed]
  • 5. Arab S, Lingwood CA. Intracellular targeting of the endoplasmic reticulum/nuclear envelope by retrograde transport may determine cell hypersensitivity to verotoxin via globotriaosyl ceramide fatty acid isoform traffic. J Cell Physiol. 1998; 177(4): 646-60[DOI][PubMed]
  • 6. Endo Y, Tsurugi K, Yutsudo T, Takeda Y, Ogasawara T, Igarashi K. Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins. Eur J Biochem. 1988; 171(1-2): 45-50[PubMed]
  • 7. Saxena SK, O'Brien AD, Ackerman EJ. Shiga toxin, Shiga-like toxin II variant, and ricin are all single-site RNA N-glycosidases of 28 S RNA when microinjected into Xenopus oocytes. J Biol Chem. 1989; 264(1): 596-601[PubMed]
  • 8. Stricklett PK, Hughes AK, Ergonul Z, Kohan DE. Molecular basis for up-regulation by inflammatory cytokines of Shiga toxin 1 cytotoxicity and globotriaosylceramide expression. J Infect Dis. 2002; 186(7): 976-82[DOI][PubMed]
  • 9. Tesh VL. Induction of apoptosis by Shiga toxins. Future Microbiol. 2010; 5(3): 431-53[DOI][PubMed]
  • 10. Akao Y, Otsuki Y, Kataoka S, Ito Y, Tsujimoto Y. Multiple subcellular localization of bcl-2: detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes. Cancer Res. 1994; 54(9): 2468-71[PubMed]
  • 11. Suzuki A, Doi H, Matsuzawa F, Aikawa S, Takiguchi K, Kawano H, et al. Bcl-2 antiapoptotic protein mediates verotoxin II-induced cell death: possible association between bcl-2 and tissue failure by E. coli O157:H7. Genes Dev. 2000; 14(14): 1734-40[PubMed]
  • 12. Jain A, Kumawat V, Patil SS, Kumar P, Marwaha N, Sharma RR. Significance of serological monitoring in a Bombay Rh (D) negative phenotype pregnant woman: a case report. Transfus Apher Sci. 2012; 47(3): 251-2[DOI][PubMed]
  • 13. Oloomi M, Bouzari S, Arshadi M. N-terminus leader sequence of Shiga toxin (Stx) 1 is essential for production of active recombinant protein in E. coli. Protein Pept Lett. 2006; 13(5): 509-12[PubMed]
  • 14. Oloomi M, Bouzari S, Ajdary S. Immune responses of mice immunized with active recombinant shiga toxin and its derivatives. Iran J Allergy Asthma Immunol. 2008; 7(2): 53-60[PubMed]
  • 15. Engedal N, Skotland T, Torgersen ML, Sandvig K. Shiga toxin and its use in targeted cancer therapy and imaging. Microb Biotechnol. 2011; 4(1): 32-46[DOI][PubMed]
  • 16. Palermo M, Alves-Rosa F, Rubel C, Fernandez GC, Fernandez-Alonso G, Alberto F, et al. Pretreatment of mice with lipopolysaccharide (LPS) or IL-1beta exerts dose-dependent opposite effects on Shiga toxin-2 lethality. Clin Exp Immunol. 2000; 119(1): 77-83[PubMed]
  • 17. Ebnet K, Vestweber D. Molecular mechanisms that control leukocyte extravasation: the selectins and the chemokines. Histochem Cell Biol. 1999; 112(1): 1-23[PubMed]
  • 18. Fujii J, Matsui T, Heatherly DP, Schlegel KH, Lobo PI, Yutsudo T, et al. Rapid apoptosis induced by Shiga toxin in HeLa cells. Infect Immun. 2003; 71(5): 2724-35[PubMed]
  • 19. Eisenhauer PB, Jacewicz MS, Conn KJ, Koul O, Wells JM, Fine RE, et al. Escherichia coli Shiga toxin 1 and TNF-alpha induce cytokine release by human cerebral microvascular endothelial cells. Microb Pathog. 2004; 36(4): 189-96[DOI][PubMed]
  • 20. Harrison LM, Cherla RP, van den Hoogen C, van Haaften WC, Lee SY, Tesh VL. Comparative evaluation of apoptosis induced by Shiga toxin 1 and/or lipopolysaccharides in human monocytic and macrophage-like cells. Microb Pathog. 2005; 38(2-3): 63-76[DOI][PubMed]
  • 21. Lee SY, Cherla RP, Caliskan I, Tesh VL. Shiga toxin 1 induces apoptosis in the human myelogenous leukemia cell line THP-1 by a caspase-8-dependent, tumor necrosis factor receptor-independent mechanism. Infect Immun. 2005; 73(8): 5115-26[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments