Bioactivity of Apium petroselinum and Portulaca oleracea Essential Oils as Natural Preservatives

AUTHORS

Reza Sharafati Chaleshtori 1 , * , Mahmoud Rafieian Kopaei 2 , Elham Salehi 1

1 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, IR Iran

2 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran

How to Cite: Sharafati Chaleshtori R, Rafieian Kopaei M, Salehi E. Bioactivity of Apium petroselinum and Portulaca oleracea Essential Oils as Natural Preservatives, Jundishapur J Microbiol. 2015 ; 8(3):e20128. doi: 10.5812/jjm.20128.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 8 (3); e20128
Published Online: January 14, 2015
Article Type: Letter
Received: May 10, 2014
Revised: August 1, 2014
Accepted: August 8, 2014
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Keywords

Antimicrobial Agents Essential oil Food Preservatives Meat

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Dear Editor,

Nowadays, essential oils are used in different products such as food as natural antioxidants, antibacterial and flavoring compounds (1). Essential oils are used as a replacement for chemical preservatives such as butylated hydroxytoluene (BHT) and tertiary butyl hydroquinone (TBHQ) that can threaten human health as cancer promoters (2). Nearly 3000 different essential oils are known today, and 300 are available and used commercially (3). They are obtained from aromatic plants and their various parts including roots, stems, leaves, flowers, fruits and seeds (4). The aim of this study was to evaluate the antioxidant activity and antibacterial effects of Apium petroselinum and Portulaca oleracea essential oils on food spoilage bacteria. A. petroselinum and P. oleracea were purchased from the local grocery stores of Shahrekord city and authenticated by standard botanic work at the Medical Plants Research Center of Shahrekord University of Medical Sciences, Iran. Essential oils were then extracted by steam distillation for three hours using a Clevenger-type apparatus and dried by adding anhydrous sodium sulfate and stored at 4°C, before being used for the assay. Total phenolic contents in the obtained essence were examined by the Folin Ciocalteu method and the results were expressed as milligrams of gallic acid equivalents (GAEs)/gram of essential oil. The total antioxidant capacities of their essential oils were assayed using β-carotene and linoleic acid (5).

Cultures of Alcaligenes faecalis (PTCC 1624), Providencia rettgeri (PTCC 1512), Serratia marcescens (PTCC 1621), Klebsiella oxytoca (PTCC 1402), Staphylococcus aureus (PTCC 1169), Shigella dysenteriae (PTCC 1188) and Listeria monocytogenes (PTCC 1163) were purchased from the Iranian Research Organization for Science and Technology (IROST). The micro-dilution method in sterile 96-microwell plates was used to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) (6). Each experiment was done in triplicates. Based on the obtained results, the two essential oils demonstrated antioxidant and antibacterial activities. The most sensitive bacteria were A. faecalis and S. dysenteriae, which are Gram-negative bacteria (Table 1). The results obtained in this work are in agreement with pervious studies. Earlier studies have reported that various bacteria such as S. dysenteriae, E. coli, Helicobacter pylori and S. aureus were sensitive to essential oils and extracts of the above mentioned plants (7, 8). However, the Gram-negative bacteria were more resistant than Gram-positive species, due to restricted diffusion of the hydrophobic compounds by the hydrophilic cell wall structure containing lipo-polysaccharide (LPS) (9). In conclusion, the study results suggest that replacement of synthetic preservatives with natural additives as a good source of healthy compounds, namely phenols (10), can be useful in the food industry to increase shelf life of food products.

Table 1. Antioxidant and Antibacterial Characteristics of Apium petroselinum and Portulaca oleracea Essential Oils a
Apium petroselinumPortulaca oleraceaBHTa
Total Phenolic Content (mg GAEs/g)8.18 ± 0.457.8 ± 0.2-
Antioxidant Capacity, %45 ± 1.5256.5 ± 4.1990.6 ± 3.30
Antibacterial activity
Alcaligenes faecalis
MIC mg/mL1.5626.25-
MBC, mg/mL3.12512.5-
Providencia rettgeri
MIC, mg/mL6.2512.5-
MBC, mg/mL12.525-
Serratia marcescens
MIC mg/mL3.12512.5-
MBC, mg/mL6.2525-
Klebsiella oxytoca
MIC, mg/mL12.512.5-
MBC, mg/mL2525-
Staphylococcus aureus
MIC, mg/mL12.512.5-
MBC, mg/mL2525-
Shigella dysenteriae
MIC, mg/mL1.5626.25-
MBC, mg/mL3.12512.5-
Listeria monocytogenes
MIC, mg/mL12.56.25-
MBC, mg/mL2512.5-

aAbbreviations: GAEs, gallic acid equivalents; BHT, butylated hydroxytoluene; MIC, minimum inhibitory concentration; MBC, minimum bactericidal concentration

Acknowledgements

Footnotes

References

  • 1.

    Shaaban HAE, El-Ghorab AH, Shibamoto T. Bioactivity of essential oils and their volatile aroma components: Review. J Essent Oil Res. 2012; 24(2) : 203 -12 [DOI]

  • 2.

    Hyldgaard M, Mygind T, Meyer RL. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol. 2012; 3 : 12 [DOI][PubMed]

  • 3.

    Burt S. Essential oils: their antibacterial properties and potential applications in foods--a review. Int J Food Microbiol. 2004; 94(3) : 223 -53 [DOI][PubMed]

  • 4.

    Sanchez E, Garcia S, Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Appl Environ Microbiol. 2010; 76(20) : 6888 -94 [DOI][PubMed]

  • 5.

    Sharafati Chaleshtori R, Sharafati Chaleshtori F, Rafieian Kopae M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turk J Biol. 2011; 35 : 635 -9

  • 6.

    Sharafati Chaleshtori R, Rokni N, Razavilar V, Rafieian Kopaei M. The Evaluation of the Antibacterial and Antioxidant Activity of Tarragon (Artemisia dracunculus L.) Essential Oil and Its Chemical Composition. Jundishapur J Microbiol. 2013; 6(9)[DOI]

  • 7.

    Nakhaei Moghaddam M. In vitro anti-bacterial activity of methanolic extract of Apium petroselinum L. seed against clinical isolates of Helicobacter pylori. Daneshvar Med. 2010; 17 : 63 -70

  • 8.

    Bae JH. Antimicrobial Effect of Portulaca oleracea Extracts on Food-Borne Pathogens. J Food Sci Nutr. 2004; 9(4) : 306 -11 [DOI]

  • 9.

    Sulaiman S, Ibrahim D, Kassim J, Sheh-Hong L. Antimicrobial and antioxidant activities of condensed tannin from Rhizophora apiculata barks. J Chem Pharm Res. 2011; 3(4) : 436 -44

  • 10.

    Rafieian-Kopaei M, Baradaran A, Rafieian M. Plants antioxidants: From laboratory to clinic. J Nephropathol. 2013; 2(2) : 152 -3 [DOI][PubMed]

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