Jundishapur Journal of Microbiology

Published by: Kowsar

Antibiotic Resistance Patterns and Genetic Diversity in Clinical Isolates of Pseudomonas aeruginosa Isolated From Patients of a Referral Hospital, Isfahan, Iran

Hamid Vaez 1 , Jamshid Faghri 1 , Bahram Nasr Esfahani 1 , Sharareh Moghim 1 , Hossein Fazeli 1 , Mansour Sedighi 1 and Hajieh Ghasemian Safaei 1 , *
Authors Information
1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Article information
  • Jundishapur Journal of Microbiology: August 01, 2015, 8 (8); e20130
  • Published Online: August 1, 2015
  • Article Type: Research Article
  • Received: May 10, 2014
  • Revised: July 30, 2014
  • Accepted: September 16, 2014
  • DOI: 10.5812/jjm.20130v2

To Cite: Vaez H, Faghri J, Nasr Esfahani B, Moghim S, Fazeli H, et al. Antibiotic Resistance Patterns and Genetic Diversity in Clinical Isolates of Pseudomonas aeruginosa Isolated From Patients of a Referral Hospital, Isfahan, Iran, Jundishapur J Microbiol. 2015 ; 8(8):e20130. doi: 10.5812/jjm.20130v2.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Hidron AI, Edwards JR, Patel J, Horan TC, Sievert DM, Pollock DA, et al. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect Control Hosp Epidemiol. 2008; 29(11): 996-1011[DOI][PubMed]
  • 2. Rossolini GM, Mantengoli E. Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clin Microbiol Infect. 2005; 11 Suppl 4: 17-32[DOI][PubMed]
  • 3. Gaynes R, Edwards JR, National Nosocomial Infections Surveillance S. Overview of nosocomial infections caused by gram-negative bacilli. Clin Infect Dis. 2005; 41(6): 848-54[DOI][PubMed]
  • 4. Bouza E, Burillo A, Munoz P. Catheter-related infections: diagnosis and intravascular treatment. Clin Microbiol Infect. 2002; 8(5): 265-74[PubMed]
  • 5. Hirsch EB, Tam VH. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev Pharmacoecon Outcomes Res. 2010; 10(4): 441-51[DOI]
  • 6. Ramirez MS, Tolmasky ME. Aminoglycoside modifying enzymes. Drug Resist Updat. 2010; 13(6): 151-71[DOI][PubMed]
  • 7. Poole K. Pseudomonas aeruginosa: resistance to the max. Front Microbiol. 2011; 2
  • 8. Pourakbari B, Sadr A, Ashtiani MT, Mamishi S, Dehghani M, Mahmoudi S, et al. Five-year evaluation of the antimicrobial susceptibility patterns of bacteria causing bloodstream infections in Iran. J Infect Dev Ctries. 2012; 6(2): 120-5[PubMed]
  • 9. Mohammadi-Mehr M, Feizabadi M. Antimicrobial resistance pattern of Gram-negative bacilli isolated from patients at ICUs of Army hospitals in Iran. Iran J Microbiol. 2011; 3(1): 26-30[PubMed]
  • 10. Hadadi A, Rasoulinejad M, Maleki Z, Yonesian M, Shirani A, Kourorian Z. Antimicrobial resistance pattern of Gram-negative bacilli of nosocomial origin at 2 university hospitals in Iran. Diagn Microbiol Infect Dis. 2008; 60(3): 301-5[DOI][PubMed]
  • 11. Haeili M, Ghodousi A, Nomanpour B, Omrani M, Feizabadi MM. Drug resistance patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-2011. J Infect Dev Ctries. 2013; 7(4): 312-7[DOI][PubMed]
  • 12. Nikokar I, Tishayar A, Flakiyan Z, Alijani K, Rehana-Banisaeed S, Hossinpour M, et al. Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa, isolated from burn patients in Guilan, Iran. Iran J Microbiol. 2013; 5(1): 36-41[PubMed]
  • 13. Khosravi AD, Mihani F. Detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran. Diagn Microbiol Infect Dis. 2008; 60(1): 125-8[DOI][PubMed]
  • 14. Singh A, Goering RV, Simjee S, Foley SL, Zervos MJ. Application of molecular techniques to the study of hospital infection. Clin Microbiol Rev. 2006; 19(3): 512-30[DOI]
  • 15. van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect. 2007; 13 Suppl 3: 1-46[DOI][PubMed]
  • 16. Picard B, Denamur E, Barakat A, Elion J, Goullet P. Genetic heterogeneity of Pseudomonas aeruginosa clinical isolates revealed by esterase electrophoretic polymorphism and restriction fragment length polymorphism of the ribosomal RNA gene region. J Med Microbiol. 1994; 40(5): 313-22[PubMed]
  • 17. Römling U, Tümmler B. Achieving 100% Typeability of Pseudomonas aeruginosaby Pulsed-Field Gel Electrophoresis. J Clin Microbiol. 2000; 38(1): 464-5
  • 18. Curran B, Jonas D, Grundmann H, Pitt T, Dowson CG. Development of a multilocus sequence typing scheme for the opportunistic pathogen Pseudomonas aeruginosa. J Clin Microbiol. 2004; 42(12): 5644-9[DOI][PubMed]
  • 19. Dawson SL, Fry JC, Dancer BN. A comparative evaluation of five typing techniques for determining the diversity of fluorescent pseudomonads. J Microbiol Methods. 2002; 50(1): 9-22[PubMed]
  • 20. Onteniente L, Brisse S, Tassios PT, Vergnaud G. Evaluation of the polymorphisms associated with tandem repeats for Pseudomonas aeruginosa strain typing. J Clin Microbiol. 2003; 41(11): 4991-7[DOI]
  • 21. Mahenthiralingam E, Campbell ME, Foster J, Lam JS, Speert DP. Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 1996; 34(5): 1129-35[PubMed]
  • 22. Kidd TJ, Grimwood K, Ramsay KA, Rainey PB, Bell SC. Comparison of Three Molecular Techniques for Typing Pseudomonas aeruginosa Isolates in Sputum Samples from Patients with Cystic Fibrosis. J Clin Microbiol. 2010; 49(1): 263-8[DOI]
  • 23. Campbell M, Mahenthiralingam E, Speert DP. Evaluation of random amplified polymorphic DNA typing of Pseudomonas aeruginosa. J Clin Microbiol. 2000; 38(12): 4614-5
  • 24. Renders N, Romling Y, Verbrugh H, van Belkum A. Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. J Clin Microbiol. 1996; 34(12): 3190-5[PubMed]
  • 25. Mahon C, Lehman D, Manuselis G. Text Book of Diagnostic Microbiology. 2011;
  • 26. Tyler SD, Strathdee CA, Rozee KR, Johnson WM. Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers. Clin Diagn Lab Immunol. 1995; 2(4): 448-53[PubMed]
  • 27. Franklin R, Cockerill III. Performance standards for antimicrobial disk susceptibility tests Approved Standards, Twenty first informational supplements, M100- S21. 2011;
  • 28. Pavlicek A, Hrda S, Flegr J. Free-Tree--freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999; 45(3): 97-9[PubMed]
  • 29. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996; 12(4): 357-8[PubMed]
  • 30. Ruimy R, Genauzeau E, Barnabe C, Beaulieu A, Tibayrenc M, Andremont A. Genetic diversity of Pseudomonas aeruginosa strains isolated from ventilated patients with nosocomial pneumonia, cancer patients with bacteremia, and environmental water. Infect Immun. 2001; 69(1): 584-8[DOI][PubMed]
  • 31. Giske CG, Libisch B, Colinon C, Scoulica E, Pagani L, Fuzi M, et al. Establishing clonal relationships between VIM-1-like metallo-beta-lactamase-producing Pseudomonas aeruginosa strains from four European countries by multilocus sequence typing. J Clin Microbiol. 2006; 44(12): 4309-15[DOI][PubMed]
  • 32. Libisch B, Balogh B, Fuzi M. Identification of two multidrug-resistant Pseudomonas aeruginosa clonal lineages with a countrywide distribution in Hungary. Curr Microbiol. 2009; 58(2): 111-6[DOI][PubMed]
  • 33. Libisch B, Watine J, Balogh B, Gacs M, Muzslay M, Szabo G, et al. Molecular typing indicates an important role for two international clonal complexes in dissemination of VIM-producing Pseudomonas aeruginosa clinical isolates in Hungary. Res Microbiol. 2008; 159(3): 162-8[DOI][PubMed]
  • 34. Czekajlo-Kolodziej U, Giedrys-Kalemba S, Medrala D. Phenotypic and genotypic characteristics of Pseudomonas aeruginosa strains isolated from hospitals in the north-west region of Poland. Pol J Microbiol. 2006; 55(2): 103-12[PubMed]
  • 35. Fazeli H, Akbari R, Moghim S, Narimani T, Arabestani MR, Ghoddousi AR. Pseudomonas aeruginosa infections in patients, hospital means, and personnel's specimens. J Res Med Sci. 2012; 17(4): 332-7[PubMed]
  • 36. Johnson JK, Arduino SM, Stine OC, Johnson JA, Harris AD. Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. J Clin Microbiol. 2007; 45(11): 3707-12[DOI][PubMed]
  • 37. Waters V, Zlosnik JE, Yau YC, Speert DP, Aaron SD, Guttman DS. Comparison of three typing methods for Pseudomonas aeruginosa isolates from patients with cystic fibrosis. Eur J Clin Microbiol Infect Dis. 2012; 31(12): 3341-50[DOI][PubMed]
  • 38. Saitou K, Furuhata K, Fukuyama M. Genotyping of Pseudomonas aeruginosa isolated from cockroaches and human urine. J Infect Chemother. 2010; 16(5): 317-21[DOI][PubMed]
  • 39. Lanotte P, Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A, et al. Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol. 2004; 53: 73-81[PubMed]
  • 40. Salimi H, Owlia P, Yakhchali B, Lari AR. Drug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn Unit. Am J Infect Dis. 2009; 5(4): 301-6
  • 41. Nanvazadeh F, Khosravi AD, Zolfaghari MR, Parhizgari N. Genotyping of Pseudomonas aeruginosa strains isolated from burn patients by RAPD-PCR. Burns. 2013; 39(7): 1409-13[DOI][PubMed]
  • 42. Matar GM, Harakeh HS, Ramlawi F, Khneisser I, Hadi U. Comparative analysis between Pseudomonas aeruginosa genotypes and severity of symptoms in patients with unilateral or bilateral otitis externa. Curr Microbiol. 2001; 42(3): 190-3[PubMed]
  • 43. Nazik H, Ongen B, Erturan Z, Salcioglu M. Genotype and antibiotic susceptibility patterns of Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolated from cystic fibrosis patients. Jpn J Infect Dis. 2007; 60(2-3): 82-6[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments