Jundishapur Journal of Microbiology

Published by: Kowsar

Detection of Methicillin-Resistant Coagulase-Negative Staphylococci and Surveillance of Antibacterial Resistance in a Multi-Center Study from Iran

Mohammad Reza Nahaei 1 , 2 , Mohammad Reza Shahmohammadi 3 , 4 , 5 , Shiva Ebrahimi 6 and Morteza Milani 1 , 7 , *
Authors Information
1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
3 Shabestar Hospital, Tabriz University of Medical Sciences, Tabriz, IR Iran
4 Aalinasab Hospital, Tabriz University of Medical Sciences, Tabriz, IR Iran
5 Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, IR Iran
6 Imam Reza Hospital, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
7 School of Advanced Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 01, 2015, 8 (8); e19945
  • Published Online: August 25, 2015
  • Article Type: Research Article
  • Received: May 3, 2014
  • Revised: June 16, 2014
  • Accepted: July 24, 2014
  • DOI: 10.5812/jjm.19945v2

To Cite: Nahaei M R, Shahmohammadi M R, Ebrahimi S, Milani M. Detection of Methicillin-Resistant Coagulase-Negative Staphylococci and Surveillance of Antibacterial Resistance in a Multi-Center Study from Iran, Jundishapur J Microbiol. 2015 ; 8(8):e19945. doi: 10.5812/jjm.19945v2.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Nnis System . National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2003, issued August 2003. Am J Infect Control. 2003; 31(8): 481-98[DOI][PubMed]
  • 2. Santucci SG, Gobara S, Santos CR, Fontana C, Levin AS. Infections in a burn intensive care unit: experience of seven years. J Hosp Infect. 2003; 53(1): 6-13[PubMed]
  • 3. Heikens E, Fleer A, Paauw A, Florijn A, Fluit AC. Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci. J Clin Microbiol. 2005; 43(5): 2286-90[DOI][PubMed]
  • 4. Otto M. Virulence factors of the coagulase-negative staphylococci. Front Biosci. 2004; 9: 841-63[PubMed]
  • 5. Rohrer S, Bischoff M, Rossi J, Berger-Bachi B. Mechanisms of methicillin resistance. 2003;
  • 6. Archer GL, Niemeyer DM. Origin and evolution of DNA associated with resistance to methicillin in staphylococci. Trends Microbiol. 1994; 2(10): 343-7[PubMed]
  • 7. Pierre J, Williamson R, Bornet M, Gutmann L. Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole. Antimicrob Agents Chemother. 1990; 34(9): 1691-4[PubMed]
  • 8. Klein E, Smith DL, Laxminarayan R. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999-2005. Emerg Infect Dis. 2007; 13(12): 1840-6[DOI][PubMed]
  • 9. Wayne P. Performance Standards for Antimicrobial Susceptibility Testing; Ninth Informational Supplement. NCCLS Document M100-S9 2008;
  • 10. Sambrook J, Russell DW. Molecular cloning: a laboratory manual. Woodbury 2001;
  • 11. Vaez H, Tabaraei A, Moradi A, Ghaemi EA. Evaluation of methicillin resistance Staphylococcus aureus isolated from patients in Golestan province-north of Iran. Afr J Microbiol Res. 2011; 5(4): 432-6
  • 12. Rahimi F, Bouzari M, Maleki Z, Rahimi F. Antibiotic susceptibility pattern among Staphylococcus spp. with emphasis on detection of mecA gene in methicillin resistant Staphylococcus aureus isolates. Arch Clin Infect Dis. 2009; 4(3): 143-50
  • 13. Rabelo MA, Bezerra Neto AM, Silva ECBF, Oliveira WLM, Melo FL, Lopes ACS, et al. Phenotypic methods for determination of methicillin resistance in Staphylococcus spp. from health care workers. Jornal Brasileiro de Patologia e Medicina Laboratorial. 2013; 49(2): 91-6[DOI]
  • 14. Najar Peerayeh S, Azimian A, Mostafaee M, Siadat SD. Identification of methicillin-resistant Staphylococcus aureus by disk diffusion method, determination of MIC and PCR for mecA gene. Modares J Med Sci Pathobiol. 2009; 12(3): 61-9
  • 15. Chambers HF. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev. 1997; 10(4): 781-91[PubMed]
  • 16. Frebourg NB, Nouet D, Lemee L, Martin E, Lemeland JF. Comparison of ATB staph, rapid ATB staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA. J Clin Microbiol. 1998; 36(1): 52-7[PubMed]
  • 17. Cavassini M, Wenger A, Jaton K, Blanc DS, Bille J. Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus. J Clin Microbiol. 1999; 37(5): 1591-4[PubMed]
  • 18. Nikbakht M, Nahaei MR, Akhi MT, Asgharzadeh M, Nikvash S. Nasal carriage rate of staphylococcus aureus in hospital personnel and inpatients and antibiotic resistance pattern of isolated strains from nasal and clinical specimens in tabriz. Med J Tabriz Uni Med Sci. 2007; 29(2)
  • 19. Brzychczy-Wloch M, Borszewska-Kornacka M, Gulczynska E, Wojkowska-Mach J, Sulik M, Grzebyk M, et al. Prevalence of antibiotic resistance in multi-drug resistant coagulase-negative staphylococci isolated from invasive infection in very low birth weight neonates in two Polish NICUs. Ann Clin Microbiol Antimicrob. 2013; 12: 41[DOI][PubMed]
  • 20. Sharma V, Jindal N, Devi P. Prevalence of methicillin resistant coagulase negative staphylococci in a tertiary care hospital. Iran J Microbiol. 2010; 2(4): 185-8[PubMed]
  • 21. Vahdani P, Saifi M, Aslani MM, Asarian AA, Sharafi K. Antibiotic resistant patterns in MRSA isolates from patients admitted in ICU and infectious ward. Tanaffos. 2004; 3(11): 37-44
  • 22. Rahimi F, Bouzari M, Katouli M, Pourshafie MR. Antibiotic Resistance Pattern of Methicillin Resistant and Methicillin Sensitive Staphylococcus aureus Isolates in Tehran, Iran. Jundishapur J Microbiol. 2013; 6(2)[DOI]
  • 23. Saderi H, Owlia P, Nadoushan MRJ. PP-007 Difference in epidemiology and antibiotic susceptibility of methicillin resistant and methicillin susceptible Staphylococcus aureus isolates. Int J Infect Dis. 2010; 14
  • 24. Diekema DJ, BootsMiller BJ, Vaughn TE, Woolson RF, Yankey JW, Ernst EJ, et al. Antimicrobial resistance trends and outbreak frequency in United States hospitals. Clin Infect Dis. 2004; 38(1): 78-85[DOI][PubMed]
  • 25. Zhang K, Sparling J, Chow BL, Elsayed S, Hussain Z, Church DL, et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol. 2004; 42(11): 4947-55[DOI][PubMed]
  • 26. Davoodi NR, Yousefi JV, Harzandi N, Hajrafi A, Rajaei B, Gerayesh-Nejad S, et al. Molecular detection of methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant coagulase-negative Staphylococcus (CoNS) in Iran. Afr J Microbiol Res. 2012; 6(16): 3716-21
  • 27. Shanthi M, Sekar U. Antimicrobial susceptibility pattern of methicillin resistant staphylococcus aureus at sri ramachandra medical centre. Sri Ramachandra J Med. 2009; 2(2)
  • 28. Moosavian M, Wadowsky R. Analysis of genomic fingerprint patterns of coagulase-negative Staphylococci strains isolated from pediatric patients blood cultures using repetitive sequence-based PCR. Jundishapur J Microbiol. 2010; 2010(4, Autumn): 147-53
  • 29. Tahnkiwale SS, Roy S, Jalgaonkar SV. Methicillin resistance among isolates of Staphylococcus aureus: antibiotic sensitivity pattern & phage typing. Indian J Med Sci. 2002; 56(7): 330-4[PubMed]
  • 30. Assadullah S, Kakru DK, Thoker MA, Bhat FA, Hussain N, Shah A. Emergence of low level vancomycin resistance in MRSA. Indian J Med Microbiol. 2003; 21(3): 196-8[PubMed]
  • 31. Ruhe JJ, Smith N, Bradsher RW, Menon A. Community-onset methicillin-resistant Staphylococcus aureus skin and soft-tissue infections: impact of antimicrobial therapy on outcome. Clin Infect Dis. 2007; 44(6): 777-84[DOI][PubMed]
  • 32. Japoni A, Alborzi A, Orafa F, Rasouli M, Farshad S. Distribution patterns of methicillin resistance genes (mecA) in Staphylococcus aureus isolated from clinical specimens. Iran Biomed J. 2004; 8(4): 173-8
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