Jundishapur Journal of Microbiology

Published by: Kowsar

Efficacy of Locally Isolated Lactic Acid Bacteria Against Antibiotic-Resistant Uropathogens

Asma Manzoor 1 , * , Ikram Ul-Haq 1 , Shahjhan Baig 2 , Javed Iqbal Qazi 3 and Sanja Seratlic 4
Authors Information
1 Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan
2 Food and Biotechnology Research Center, PCSIR Labs Complex, Lahore, Pakistan
3 Department of Zoology, University of the Punjab, Lahore, Pakistan
4 Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland
Article information
  • Jundishapur Journal of Microbiology: January 01, 2016, 9 (1); e18952
  • Published Online: January 16, 2016
  • Article Type: Research Article
  • Received: March 15, 2014
  • Revised: June 14, 2014
  • Accepted: June 28, 2014
  • DOI: 10.5812/jjm.18952

To Cite: Manzoor A, Ul-Haq I, Baig S, Qazi J I, Seratlic S. Efficacy of Locally Isolated Lactic Acid Bacteria Against Antibiotic-Resistant Uropathogens, Jundishapur J Microbiol. 2016 ; 9(1):e18952. doi: 10.5812/jjm.18952.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Aslam S, Qazi JI. Isolation of acidophilic lactic acid bacteria antagonistic to microbial contaminants. Pakistan J Zool. 2010; 42(5): 567-73
  • 2. Kucheria R, Dasgupta P, Sacks SH, Khan MS, Sheerin NS. Urinary tract infections: new insights into a common problem. Postgrad Med J. 2005; 81(952): 83-6[DOI][PubMed]
  • 3. Reid G. Probiotic agents to protect the urogenital tract against infection. Am J Clin Nutr. 2001; 73(2 Suppl): 437S-43S[PubMed]
  • 4. Arredondo-Garcia JL, Amabile-Cuevas CF. High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City. J Infect Dev Ctries. 2008; 2(5): 350-3[PubMed]
  • 5. Munoz-Atienza E, Gomez-Sala B, Araujo C, Campanero C, del Campo R, Hernandez PE, et al. Antimicrobial activity, antibiotic susceptibility and virulence factors of Lactic Acid Bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC Microbiol. 2013; 13: 15[DOI][PubMed]
  • 6. Asahara T, Nomoto K, Watanuki M, Yokokura T. Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection. Antimicrob Agents Chemother. 2001; 45(6): 1751-60[DOI][PubMed]
  • 7. Defoirdt T, Sorgeloos P, Bossier P. Alternatives to antibiotics for the control of bacterial disease in aquaculture. Curr Opin Microbiol. 2011; 14(3): 251-8[DOI][PubMed]
  • 8. Evrard B, Coudeyras S, Dosgilbert A, Charbonnel N, Alame J, Tridon A, et al. Dose-dependent immunomodulation of human dendritic cells by the probiotic Lactobacillus rhamnosus Lcr35. PLoS One. 2011; 6(4)[DOI][PubMed]
  • 9. Okereke HC. Antimicrobial properties of probiotic bacteria from various sources. Afr J Biotech. 2012; 11(39): 9416-21[DOI]
  • 10. Lakshminarayanan B, Guinane CM, O'Connor PM, Coakley M, Hill C, Stanton C, et al. Isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly Irish subjects. J Appl Microbiol. 2013; 114(3): 886-98[DOI][PubMed]
  • 11. Goldin BR, Gorbach SL. Clinical indications for probiotics: an overview. Clin Infect Dis. 2008; 46 Suppl 2-100[DOI][PubMed]
  • 12. Marja T, Arthur C, Ouwehand , Tiina J, Maarit M. Viability of Lactobacillus paraplantarum DSM 14485 in human gastrointestinal tract and its molecular and biochemical identification after fermented vegetable consumption. Agri Food Sci. 2012; 21: 182-96
  • 13. De Man JC, Rogosa M, Sharpe ME. A Medium for the Cultivation of Lactobacilli. J Appl Bacteriol. 1960; 23(1): 130-5[DOI]
  • 14. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergeys Manual of Determinative Bacteriology. 2000;
  • 15. Maniatis T, Fritsh EF, Sambrook J. A laboratory manual. 1982;
  • 16. Randazzo CL, Torriani S, Akkermans AD, de Vos WM, Vaughan EE. Diversity, dynamics, and activity of bacterial communities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis. Appl Environ Microbiol. 2002; 68(4): 1882-92[PubMed]
  • 17. Lawalata HJ, Sembiring L, Rahayu ES. Molecular identification of lactic acid bacteria producing antimicrobial agents from Bakasang, an Indonesian traditional fermented fish product. Indonesian J Biotech. 2012; 16(2): 93-9
  • 18. CLSI. . CLSI Performance Standards for Antimicrobial Susceptibility Testing: Twenty–first Informational Supplement M100–S21. USA; Wayne PA. 2011;
  • 19. Milk and milk products determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria non-enterococcal lactic acid bacteria (LAB). 2010;
  • 20. Pal V, Jamuna M, Jeevaratnam K. Isolation and characterization of bacteriocin producing lactic acid bacteria from a south indian special dosa (Appam) batter. J Culture Collect. 2005; 4: 53-60
  • 21. EFSA-FEEDAP. . Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA J. 2012; 10: 2740-9
  • 22. Mallesha R, Shylaja D, Selvakumar D, Jagannath JH. Isolation and identification of lactic acid bacteria from raw and fermented products and their antibacterial activity. Recent Res Sci Tech. 2010; 2(6): 42-6
  • 23. Ayeni FA, Adeniyi BA, Ogunbanwo ST, Tabasco R, Paarup T, Pelaez C, et al. Inhibition of uropathogens by lactic acid bacteria isolated from dairy foods and cow's intestine in western Nigeria. Arch Microbiol. 2009; 191(8): 639-48[DOI][PubMed]
  • 24. Margolles A, Mayo B, Ruas-Madiedo P. Handbook of Probiotics and Prebiotics. 2009; : 4-43
  • 25. Boyd MA, Antonio MA, Hillier SL. Comparison of API 50 CH strips to whole-chromosomal DNA probes for identification of Lactobacillus species. J Clin Microbiol. 2005; 43(10): 5309-11[DOI][PubMed]
  • 26. Sarika AR, Lipton AP, Aishwarya MS. Bacteriocin production by a new isolate of Lactobacillus rhamnosus GP1 under different culture conditions. Adv J Food Sci Technol. 2010; 2(5): 291-7
  • 27. Lim IS, Lee HS, Kim WY. The effect of lactic acid bacteria isolates on the urinary tract pathogens to infants in vitro. J Korean Med Sci. 2009; 24 Suppl-62[DOI][PubMed]
  • 28. Hooton TM, Stamm WE. Diagnosis and treatment of uncomplicated urinary tract infection. Infect Dis Clin North Am. 1997; 11(3): 551-81[PubMed]
  • 29. Klare I, Konstabel C, Werner G, Huys G, Vankerckhoven V, Kahlmeter G, et al. Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use. J Antimicrob Chemother. 2007; 59(5): 900-12[DOI][PubMed]
  • 30. Klein G, Hallmann C, Casas IA, Abad J, Louwers J, Reuter G. Exclusion of vanA, vanB and vanC type glycopeptide resistance in strains of Lactobacillus reuteri and Lactobacillus rhamnosus used as probiotics by polymerase chain reaction and hybridization methods. J Appl Microbiol. 2000; 89(5): 815-24[PubMed]
  • 31. O'Shea EF, Cotter PD, Stanton C, Ross RP, Hill C. Production of bioactive substances by intestinal bacteria as a basis for explaining probiotic mechanisms: bacteriocins and conjugated linoleic acid. Int J Food Microbiol. 2012; 152(3): 189-205[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments