Jundishapur Journal of Microbiology

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Genetic Characterization of Campylobacter Jejuni and C. coli Isolated From Broilers Using flaA PCR-Restriction Fragment Length Polymorphism Method in Shiraz, Southern Iran

Rahem Khoshbakht 1 , * , Mohammad Tabatabaei 2 , Saeid Hosseinzadeh 3 , Hesamaddin Shirzad Aski 2 and Saeed Seifi 1
Authors Information
1 Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, IR Iran
2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, IR Iran
3 Department of Public Health and Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: May 01, 2015, 8 (5); e18573
  • Published Online: May 31, 2015
  • Article Type: Research Article
  • Received: February 25, 2014
  • Revised: June 13, 2014
  • Accepted: July 21, 2014
  • DOI: 10.5812/jjm.8(5)2015.18573

To Cite: Khoshbakht R, Tabatabaei M, Hosseinzadeh S, Shirzad Aski H, Seifi S. Genetic Characterization of Campylobacter Jejuni and C. coli Isolated From Broilers Using flaA PCR-Restriction Fragment Length Polymorphism Method in Shiraz, Southern Iran, Jundishapur J Microbiol. 2015 ; 8(5):e18573. doi: 10.5812/jjm.8(5)2015.18573.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Backgrounds
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnote
References
  • 1. Ertas HB, Cetinkaya B, Muz A, Ongor H. Genotyping of broiler-originated Campylobacter jejuni and Campylobacter coli isolates using fla typing and random amplified polymorphic DNA methods. Int J Food Microbiol. 2004; 94(2): 203-9[DOI][PubMed]
  • 2. Stern NJ, Fedorka-Cray P, Bailey JS, Cox NA, Craven SE, Hiett KL, et al. Distribution of Campylobacter spp. in selected U.S. poultry production and processing operations. J Food Prot. 2001; 64(11): 1705-10[PubMed]
  • 3. Stoyanchev T, Vashin I, Ring C, Atanassova V. Prevalence of Campylobacter spp. in poultry and poultry products for sale on the Bulgarian retail market. Antonie Van Leeuwenhoek. 2007; 92(3): 285-8[DOI][PubMed]
  • 4. Mdegela RH, Laurence K, Jacob P, Nonga HE. Occurrences of thermophilic Campylobacter in pigs slaughtered at Morogoro slaughter slabs, Tanzania. Trop Anim Health Prod. 2011; 43(1): 83-7[DOI][PubMed]
  • 5. Engvall EO, Brandstrom B, Gunnarsson A, Morner T, Wahlstrom H, Fermer C. Validation of a polymerase chain reaction/restriction enzyme analysis method for species identification of thermophilic campylobacters isolated from domestic and wild animals. J Appl Microbiol. 2002; 92(1): 47-54[PubMed]
  • 6. Wassenaar TM, Newell DG. Genotyping of Campylobacter spp. Appl Environ Microbiol. 2000; 66(1): 1-9[PubMed]
  • 7. Caner V, Cokal Y, Cetin C, Sen A, Karagenc N. The detection of hipO gene by real-time PCR in thermophilic Campylobacter spp. with very weak and negative reaction of hippurate hydrolysis. Antonie Van Leeuwenhoek. 2008; 94(4): 527-32[DOI][PubMed]
  • 8. Findik A, Ica T, Onuk EE, Percin D, Kevenk TO, Ciftci A. Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources. Trop Anim Health Prod. 2011; 43(3): 711-9[DOI][PubMed]
  • 9. Corcoran D, Quinn T, Cotter L, Whyte P, Fanning S. Antimicrobial resistance profiling and fla-typing of Irish thermophillic Campylobacter spp. of human and poultry origin. Lett Appl Microbiol. 2006; 43(5): 560-5[DOI][PubMed]
  • 10. Shi F, Chen YY, Wassenaar TM, Woods WH, Coloe PJ, Fry BN. Development and application of a new scheme for typing Campylobacter jejuni and Campylobacter coli by PCR-based restriction fragment length polymorphism analysis. J Clin Microbiol. 2002; 40(5): 1791-7[PubMed]
  • 11. Ansari-Lari M, Hosseinzadeh S, Shekarforoush SS, Abdollahi M, Berizi E. Prevalence and risk factors associated with campylobacter infections in broiler flocks in Shiraz, southern Iran. Int J Food Microbiol. 2011; 144(3): 475-9[DOI][PubMed]
  • 12. Khoshbakht R, Tabatabaei M, Hosseinzadeh S, Shekarforoush SS, Aski HS. Distribution of nine virulence-associated genes in Campylobacter jejuni and C. coli isolated from broiler feces in Shiraz, Southern Iran. Foodborne Pathog Dis. 2013; 10(9): 764-70[DOI][PubMed]
  • 13. Nachamkin I, Bohachick K, Patton CM. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol. 1993; 31(6): 1531-6[PubMed]
  • 14. Denis M, Soumet C, Rivoal K, Ermel G, Blivet D, Salvat G, et al. Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli. Lett Appl Microbiol. 1999; 29(6): 406-10[PubMed]
  • 15. Cardarelli-Leite P, Blom K, Patton CM, Nicholson MA, Steigerwalt AG, Hunter SB, et al. Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA. J Clin Microbiol. 1996; 34(1): 62-7[PubMed]
  • 16. Harrington CS, Moran L, Ridley AM, Newell DG, Madden RH. Inter-laboratory evaluation of three flagellin PCR/RFLP methods for typing Campylobacter jejuni and C. coli: the CAMPYNET experience. J Appl Microbiol. 2003; 95(6): 1321-33[PubMed]
  • 17. Wieczorek K, Osek J. Identification of virulence genes in campylobacter jejuni and c. coli isolates by PCR. Bull Vet Inst Pulawy. 2008; 52(1): 211-6
  • 18. El-Adawy H, Hotzel H, Tomaso H, Neubauer H, Taboada EN, Ehricht R, et al. Detection of genetic diversity in Campylobacter jejuni isolated from a commercial turkey flock using flaA typing, MLST analysis and microarray assay. PLoS One. 2013; 8(2)[DOI][PubMed]
  • 19. Fitzgerald C, Stanley K, Andrew S, Jones K. Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of Campylobacter jejuni and Campylobacter coli in farm and clinical environments. Appl Environ Microbiol. 2001; 67(4): 1429-36[DOI][PubMed]
  • 20. Bang DD, Nielsen EM, Scheutz F, Pedersen K, Handberg K, Madsen M. PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. J Appl Microbiol. 2003; 94(6): 1003-14[PubMed]
  • 21. Acik MN, Cetinkaya B. The heterogeneity of Campylobacter jejuni and Campylobacter coli strains isolated from healthy cattle. Lett Appl Microbiol. 2005; 41(5): 397-403[DOI][PubMed]
  • 22. Oporto B, Esteban JI, Aduriz G, Juste RA, Hurtado A. Prevalence and strain diversity of thermophilic campylobacters in cattle, sheep and swine farms. J Appl Microbiol. 2007; 103(4): 977-84[DOI][PubMed]
  • 23. Datta S, Niwa H, Itoh K. Age-dependent variation of virulence-associated genes retained in Campylobacter jejuni isolated from chickens in a poultry farm. J Vet Med Sci. 2009; 71(9): 1247-9[PubMed]
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