Jundishapur Journal of Microbiology

Published by: Kowsar

Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran

Rashid Ramazanzadeh 1 , 2 , Samaneh Rouhi 3 , Pegah Shakib 3 , Babak Shahbazi 2 , * , Farzam Bidarpour 4 and Mohammad Karimi 4
Authors Information
1 Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
2 Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
3 Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
4 Deputy of Public Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
Article information
  • Jundishapur Journal of Microbiology: May 01, 2015, 8 (5); e18119
  • Published Online: May 31, 2015
  • Article Type: Research Article
  • Received: February 9, 2014
  • Revised: April 12, 2014
  • Accepted: April 23, 2014
  • DOI: 10.5812/jjm.8(5)2015.18119

To Cite: Ramazanzadeh R, Rouhi S, Shakib P, Shahbazi B, Bidarpour F, et al. Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran, Jundishapur J Microbiol. 2015 ; 8(5):e18119. doi: 10.5812/jjm.8(5)2015.18119.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Fiore AE, Michalski JM, Russell RG, Sears CL, Kaper JB. Cloning, characterization, and chromosomal mapping of a phospholipase (lecithinase) produced by Vibrio cholerae. Infect Immun. 1997; 65(8): 3112-7[PubMed]
  • 2. Tracz DM, Backhouse PG, Olson AB, McCrea JK, Walsh JA, Ng LK, et al. Rapid detection of Vibrio species using liquid microsphere arrays and real-time PCR targeting the ftsZ locus. J Med Microbiol. 2007; 56: 56-65[DOI][PubMed]
  • 3. Faruque SM, Asadulghani N, Rahman MM, Waldor MK, Sack DA. Sunlight-induced propagation of the lysogenic phage encoding cholera toxin. Infect Immun. 2000; 68(8): 4795-801[PubMed]
  • 4. Goss TJ, Morgan SJ, French EL, Krukonis ES. ToxR recognizes a direct repeat element in the toxT, ompU, ompT, and ctxA promoters of Vibrio cholerae to regulate transcription. Infect Immun. 2013; 81(3): 884-95[DOI][PubMed]
  • 5. Alizadeh J, Ranjbar R, Kamali M, Farhadi N, Davari A, Sadeghifard N. Cloning of Vibrio cholerae outer membrane protein W in Pichia pastoris. Iran J Microbiol. 2013; 5(3): 252-8[PubMed]
  • 6. Talkington D, Bopp C, Tarr C, Parsons MB, Dahourou G, Freeman M, et al. Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011. Emerg Infect Dis. 2011; 17(11): 2122-9[DOI][PubMed]
  • 7. Harwood VJ, Gandhi JP, Wright AC. Methods for isolation and confirmation of Vibrio vulnificus from oysters and environmental sources: a review. J Microbiol Methods. 2004; 59(3): 301-16[DOI][PubMed]
  • 8. Hu X, Zhang Y, Zhou X, Xu B, Yang M, Wang M, et al. Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyzer-based multiplex reverse transcription-PCR assay. J Clin Microbiol. 2012; 50(2): 288-93[DOI][PubMed]
  • 9. Waturangi DE, Joanito I, Yogi Y, Thomas S. Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia. Gut Pathog. 2012; 4(1): 2[DOI][PubMed]
  • 10. Caburlotto G, Lleo MM, Gennari M, Balboa S, Romalde JL. The use of multiple typing methods allows a more accurate molecular characterization of Vibrio parahaemolyticus strains isolated from the Italian Adriatic Sea. FEMS Microbiol Ecol. 2011; 77(3): 611-22[DOI][PubMed]
  • 11. Alishahi A, Imani Fooladi AA, Fallah Mehrabadi J, Mahmoodzadeh Hosseini H. Facile and Rapid Detection of Vibrio cholerae by Multiplex PCR Based on ompU, ctxA, and toxR Genes. Jundishapur J Microbiol. 2013; 6(10)[DOI]
  • 12. Naha A, Chowdhury G, Ghosh-Banerjee J, Senoh M, Takahashi T, Ley B, et al. Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania. J Clin Microbiol. 2013; 51(3): 1040-5[DOI][PubMed]
  • 13. Mancuso M, Avendano-Herrera R, Zaccone R, Toranzo AE, Magarinos B. Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida. Biol Res. 2007; 40(1): 85-92[PubMed]
  • 14. Madhusudana Rao B, Surendran PK. Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches. Lett Appl Microbiol. 2010; 51(1): 65-74
  • 15. Global Disease Detection (GDD) Manual Rapid Diagnostic Tests for Epidemic Diseases. 2011;
  • 16. Sharma A, Chaturvedi AN. Prevalence of virulence genes (ctxA, stn, OmpW and tcpA) among non-O1 Vibrio cholerae isolated from fresh water environment. Int J Hyg Environ Health. 2006; 209(6): 521-6[DOI][PubMed]
  • 17. Goel AK, Jiang SC. Genetic determinants of virulence, antibiogram and altered biotype among the Vibrio cholerae O1 isolates from different cholera outbreaks in India. Infect Genet Evol. 2010; 10(6): 815-9[DOI][PubMed]
  • 18. Gubala AJ, Proll DF. Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae. Appl Environ Microbiol. 2006; 72(9): 6424-8[DOI][PubMed]
  • 19. Maleki A, Pakzad I, Hosseini S, Ranjbar R, Purshafie M, Mohebi R, et al. Frequency Analysis of Genes ctxA, tcpA, Ace and Zot in Vibrio Cholerae Isolated of Epidemy of Summer 2005 in Iran By PCR. Sci J Ilam Univ Med Sci. 2011; 18(4): 1-6
  • 20. Schirmeister F, Dieckmann R, Bechlars S, Bier N, Faruque SM, Strauch E. Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 isolated from German and Austrian patients. Eur J Clin Microbiol Infect Dis. 2014; 33(5): 767-78[DOI][PubMed]
  • 21. Epidemiology, Prevention and Control of Cholera in Hong Kong. 2011;
  • 22. Cholera (Toxigenic Vibrio cholerae O1, O139). Guide to Surveillance and Investigation. 2011;
  • 23. Pourshafie M, Bakhshi B, Ranjbar R, Sedaghat M, Sadeghifard N, Yazdi J, et al. Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran. J Med Microbiol. 2007; 56(12): 1615-9
  • 24. Srisuk C, Chaivisuthangkura P, Rukpratanporn S, Longyant S, Sridulyakul P, Sithigorngul P. Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompW. Lett Appl Microbiol. 2010; 50(1): 36-42[DOI][PubMed]
  • 25. Yildirim I. Molecular methods for bacterial genotyping and analyzed gene regions. J Microbiol Infect Dis. 2011; 1(1): 42-6[DOI]
  • 26. Goel AK, Jain M, Kumar P, Jiang SC. Molecular characterization of Vibrio cholerae outbreak strains with altered El Tor biotype from southern India. World J Microbiol Biotechnol. 2010; 26(2): 281-7[DOI][PubMed]
  • 27. Taneja N, Sangar G, Chowdhury G, Ramamurthy T, Mishra A, Singh M, et al. Molecular epidemiology of Vibrio cholerae causing outbreaks & sporadic cholera in northern India. Indian J Med Res. 2012; 136(4): 656-63[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments