Jundishapur Journal of Microbiology

Published by: Kowsar

Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli

Zahra Shoja 1 , Hamid Rajabi Memari 2 , * and Mohammd Roayaei Ardakani 3
Authors Information
1 Biology Department, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran
2 Agronomy and Plant Breeding Department, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
3 Biology Department, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 01, 2015, 8 (8); e17809
  • Published Online: August 29, 2015
  • Article Type: Research Article
  • Received: January 24, 2014
  • Revised: May 3, 2014
  • Accepted: June 2, 2014
  • DOI: 10.5812/jjm.17809

To Cite: Shoja Z, Rajabi Memari H, Roayaei Ardakani M. Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli, Jundishapur J Microbiol. 2015 ; 8(8):e17809. doi: 10.5812/jjm.17809.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Footnote
References
  • 1. Cevallos GC, Barron BL, Sánchez JV. Spirulina in Human Nutrition and Health. 2008;
  • 2. Andrade MR, Costa JAV. Mixotrophic cultivation of microalga Spirulina platensis using molasses as organic substrate. Aquaculture. 2007; 264(1): 130-4
  • 3. Nomsawai P, De-Marsac NT, Thomas JC, Tanticharoen M, Cheevadhanarak S. Light regulation of phycobilisome structure and gene expression in Spirulina platensis C1 (Arthrospira sp. PCC 9438). Plant Cell Physiol. 1999; 40(12): 1194-202
  • 4. Tooley AJ, Cai YA, Glazer AN. Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host. Proc Natl Acad Sci U S A. 2001; 98(19): 10560-5[DOI][PubMed]
  • 5. Guan X, Qin S, Su Z, Zhao F, Ge B, Li F, et al. Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector. Appl Biochem Biotechnol. 2007; 142(1): 52-9[PubMed]
  • 6. Sekar S, Chandramohan M. Phycobiliproteins as a commodity: trends in applied research, patents and commercialization. J Appl Phycol. 2008; 20(2): 113-36
  • 7. Jeamton W, Dulsawat S, Laoteng K, Tanticharoen M, Cheevadhanarak S. Phycocyanin promoter of Spirulina platensis controlling heterologous expression in cyanobacteria. J Appl Phycol. 2010; 23(1): 83-8[DOI]
  • 8. Silveira ST, Quines LK, Burkert CA, Kalil SJ. Separation of phycocyanin from Spirulina platensis using ion exchange chromatography. Bioprocess Biosyst Eng. 2008; 31(5): 477-82[DOI][PubMed]
  • 9. Romay C, Gonzalez R, Ledon N, Remirez D, Rimbau V. C-phycocyanin: a biliprotein with antioxidant, anti-inflammatory and neuroprotective effects. Curr Protein Pept Sci. 2003; 4(3): 207-16[PubMed]
  • 10. Deng R, Chow TJ. Hypolipidemic, antioxidant, and antiinflammatory activities of microalgae Spirulina. Cardiovasc Ther. 2010; 28(4)-45[DOI][PubMed]
  • 11. Patel A, Mishra S, Ghosh PK. Antioxidant potential of C-phycocyanin isolated from cyanobacterial species Lyngbya, Phormidium and Spirulina spp. Indian J Biochem Biophys. 2006; 43(1): 25-31[PubMed]
  • 12. Vadiraja BB, Gaikwad NW, Madyastha KM. Hepatoprotective effect of C-phycocyanin: protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats. Biochem Biophys Res Commun. 1998; 249(2): 428-31[DOI][PubMed]
  • 13. Liu Y, Xu L, Cheng N, Lin L, Zhang C. Inhibitory effect of phycocyanin from Spirulina platensis on the growth of human leukemia K562 cells. J Appl Phycol. 2000; 12(2): 125-30[DOI]
  • 14. Pardhasaradhi BV, Ali AM, Kumari AL, Reddanna P, Khar A. Phycocyanin-mediated apoptosis in AK-5 tumor cells involves down-regulation of Bcl-2 and generation of ROS. Mol Cancer Ther. 2003; 2(11): 1165-70[PubMed]
  • 15. Subhashini J, Mahipal SV, Reddy MC, Mallikarjuna Reddy M, Rachamallu A, Reddanna P. Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. Biochem Pharmacol. 2004; 68(3): 453-62[DOI][PubMed]
  • 16. Wang H, Liu Y, Gao X, Carter CL, Liu ZR. The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis. Cancer Lett. 2007; 247(1): 150-8[DOI][PubMed]
  • 17. Gholipour A, Moosavian M, Galehdari H, Makvandi M, Memari HR, Alvandi A. Cloning and periplasmic expression of peptidoglycan-associated lipoprotein (PAL) protein of Legionella pneumophila in Escherichia coli. Jundishapur J Microbiol. 2010; 3(1): 1-9
  • 18. Ramanan RN, Tik WB, Memari HR, Azaman SNA, Ling T, Tey BT, et al. Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-a2b in Escherichia coli. Afr J Biotechnol. 2010; 9(3)
  • 19. Morowvat MH, Babaeipour V, Rajabi-Memari H, Vahidi H. Metabolic changes of recombinant Escherichia coli BL21 (DE3) during overexpression of recombinant human interferon beta in HCDC. Int J Biosci. 2014; 4(4): 131-8
  • 20. Morowvat MH, Babaeipour V, Rajabi-Memari H, Vahidi H, Maghsoudi N. Overexpression of Recombinant Human Beta Interferon (rhINF-beta) in Periplasmic Space of Escherichia coli. Iran J Pharm Res. 2014; 13: 151-60[PubMed]
  • 21. Sorensen HP, Mortensen KK. Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol. 2005; 115(2): 113-28[DOI][PubMed]
  • 22. Raoof B, Kaushik BD, Prasanna R. Formulation of a low-cost medium for mass production of Spirulina. Biomass Bioenergy. 2006; 30(6): 537-42[DOI]
  • 23. Berrendero E, Perona E, Mateo P. Genetic and morphological characterization of Rivularia and Calothrix (Nostocales, Cyanobacteria) from running water. Int J Syst Evol Microbiol. 2008; 58: 447-60[DOI][PubMed]
  • 24. Sambrook J, Russel DW. Molecular cloning, a laboratory manual. 2001;
  • 25. Abdolrasouli N, Memari HR, Ardakani MR, Ebrahimi MA, Ebrahimi N. Production of Human Alpha 2b Interferon in E. coli Expression System. Jundishapur Sci Med J. 2013; 12(3): 325-34
  • 26. Lab FAQS - find a quick solution. 2011;
  • 27. Ge B, Qin S, Han L, Lin F, Ren Y. Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli. J Photochem Photobiol B. 2006; 84(3): 175-80[DOI][PubMed]
  • 28. Bryant DA, Dubbs JM, Fields PI, Porter RD, Lorimier R. Expression of phycobiliprotein genes in Escherichia coli. FEMS Microbiol Letters. 1985; 29(3): 343-9[DOI]
  • 29. Ge B, Tang Z, Lin L, Ren Y, Yang Y, Qin S. Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli. Biotechnol Lett. 2005; 27(11): 783-7[DOI][PubMed]
  • 30. Ge B, Tang Z, Zhao F, Ren Y, Yang Y, Qin S. Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli. Process Biochem. 2005; 40(10): 3190-5[DOI]
  • 31. Tartof KD, Hobbs CA. Improved media for growing plasmid and cosmid clones. Focus. 1987; 9(2): 12
  • 32. Liu S, Chen H, Qin S, Zhang W, Guan X, Lu Y. Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli. Biochem E J. 2009; 48(1): 58-64[DOI]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments