Jundishapur Journal of Microbiology

Published by: Kowsar

Pseudomonas aeruginosa PAO-1 Lipopolysaccharide-Diphtheria Toxoid Conjugate Vaccine: Preparation, Characterization and Immunogenicity

Faezeh Najafzadeh 1 , Reza Shapouri 2 , Mehdi Rahnema 3 , Shadi Rokhsartalab Azar 4 and Anvarsadat Kianmehr 5 , *
Authors Information
1 Young Researchers Club, Bonab Branch, Islamic Azad University, Bonab, IR Iran
2 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran
3 Biologic Research Center, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran
4 Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, IR Iran
5 Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: June 01, 2015, 8 (6); e17712
  • Published Online: June 27, 2015
  • Article Type: Research Article
  • Received: January 18, 2014
  • Revised: May 26, 2014
  • Accepted: June 7, 2014
  • DOI: 10.5812/jjm.8(5)2015.17712

To Cite: Najafzadeh F, Shapouri R, Rahnema M, Rokhsartalab Azar S, Kianmehr A. Pseudomonas aeruginosa PAO-1 Lipopolysaccharide-Diphtheria Toxoid Conjugate Vaccine: Preparation, Characterization and Immunogenicity, Jundishapur J Microbiol. 2015 ; 8(6):e17712. doi: 10.5812/jjm.8(5)2015.17712.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
References
  • 1. Siegel RE. Emerging gram-negative antibiotic resistance: daunting challenges, declining sensitivities, and dire consequences. Respir Care. 2008; 53(4): 471-9
  • 2. Taheri ZM, Shahbazi N, Khoddami M. Genetic Diversity of pseudomonas aeruginosa Strains isolated from Hospitalized patients. Tanaffos. 2008; 7(1): 32-9
  • 3. Al-Zeer M, Masoud H. LPS-based conjugate vaccines composed of O-polysaccharide from Pseudomonas aeruginosa IATS 6 and 11 bound to a carrier protein. World J Microbiol Biotechnol . 2007; 23(11): 1541-9
  • 4. Donta ST, Peduzzi P, Cross AS, Sadoff J, Haakenson C, Cryz SJ, et al. Immunoprophylaxis against Klebsiella and Pseudomonas aeruginosa Infections. J Infect Dis. 1996; 174(3): 537-43
  • 5. Cryz SJJ, Lang AB, Sadoff JC, Germanier R, Furer E. Vaccine potential of Pseudomonas aeruginosa O-polysaccharide-toxin A conjugates. Infect Immun. 1987; 55(7): 1547-51[PubMed]
  • 6. Cryz SJ, Sadoff JC, Fiirer E, Germanier R. Pseudomonas aeruginosa Polysaccharide-Tetanus Toxoid Conjugate Vaccine: Safety and Immunogenicity in Humans. J Infect Dis. 1986; 154(4): 682-8
  • 7. Robinson A, Hudson MJ, Cranage MP. Vaccine Protocols. 2000;
  • 8. Midwinter A, Faine S, Adler B. Vaccination of mice with lipopolysaccharide (LPS) and LPS-derived immuno-conjugates from Leptospira interrogans. J Med Microbiol. 1990; 33(3): 199-204
  • 9. Aspe M, Jensen L, Melegrito J, Sun M. The role of alginate and extracellular DNA in biofilm-meditated Pseudomonas aeruginosa gentamicin resistance. J Exp Microbiol Immunol. 2012; 16: 42-8
  • 10. Kashef N. Synthesis and characterization of Pseudomonas aeruginosa alginate-tetanus toxoid conjugate. J Med Microbiol. 2006; 55(10): 1441-6
  • 11. Konadu E, Robbins JB, Shiloach J, Bryla DA, Szu SC. Preparation, characterization, and immunological properties in mice of Escherichia coli O157 O-specific polysaccharide-protein conjugate vaccines. Infect Immun. 1994; 62(11): 5048-54[PubMed]
  • 12. Cryz SJ, Furer E, Cross AS, Wegmann A, Germanier R, Sadoff JC. Safety and immunogenicity of a Pseudomonas aeruginosa O-polysaccharide toxin A conjugate vaccine in humans. J Clin Invest. 1987; 80(1): 51-6[DOI][PubMed]
  • 13. Zeng X, Liu X, Bian J, Pei G, Dai H, Polyak SW, et al. Synergistic effect of 14-alpha-lipoyl andrographolide and various antibiotics on the formation of biofilms and production of exopolysaccharide and pyocyanin by Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2011; 55(6): 3015-7[DOI][PubMed]
  • 14. Kunert A, Losse J, Gruszin C, Huhn M, Kaendler K, Mikkat S, et al. Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein. J Immunol. 2007; 179(5): 2979-88[PubMed]
  • 15. Cryz SJ, Furer E, Germanier R. Protection against fatal Pseudomonas aeruginosa burn wound sepsis by immunization with lipopolysaccharide and high-molecular-weight polysaccharide. Infect Immun. 1984; 43(3): 795-9[PubMed]
  • 16. Baumann U, Mansouri E, von Specht BU. Recombinant OprF-OprI as a vaccine against Pseudomonas aeruginosa infections. Vaccine. 2004; 22(7): 840-7[DOI][PubMed]
  • 17. Collier L, Balows A, Sussman M. Topley & Wilson's microbiology and microbial infections. 1998;
  • 18. Cryz SJ, Sadoff JC, Fürer E. Octavalent Pseudomonas aeruginosa O-polysaccharide-toxin A conjugate vaccine. Microb Pathog. 1989; 6(1): 75-80
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments