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Airborne Fungi in Tabriz, Comparing Airborne and Clinical Samples of A. fumigatus (2011), Survey and Literature Review

AUTHORS

Abdolhassan Kazemi 1 , Ehssan Ahmadpour 1 , Behroz Naghili 1 , Ali Zarei Mahmoudabadi 2 , Abbasali Jafari 3 , * , Amin Mousavi Ayatollahi 4

AUTHORS INFORMATION

1 Infection and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran

2 Department of Medical Mycology, and Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

3 Department of Medical Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran, Yazd, IR Iran

4 Department of Medical Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 6 (4); e4997
Published Online: June 10, 2013
Article Type: Research Article
Received: April 4, 2012
Revised: June 19, 2012
Accepted: July 9, 2012
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Abstract

Background: Air contamination with fungal spores and the presence of these spores on respiratory tract, especially in industrialized cities with contaminated air, can play an important role on the occurrence of respiratory and coetaneous mycoses, asthma and allergic reactions. This survey was carried out to determine the prevalence of different fungal spores in the atmosphere of Tabriz district.

Objectives: The present study aimed to detect fungal air spores in Tabriz environments, and to compare the environmental samples of Aspergillus fumigatus with the clinical isolated samples of this fungus, due to the importance of the dangers of A. fumigatus for public health, particularly for the immunocompromised patients.

Materials and Methods: During this survey, the presence of air fungal spores was analyzed using settle plate and prepared culture in Sabouraud’s dextrose agar. Prior identifications were performed using macroscopic characters, and direct microscopy. 262 samples were collected from different areas of the atmosphere of Tabriz district within all four seasons of the year. Fungal colonies were isolated from all air samples and identified using macroscopic and microscopic characters, and slid culture.

Results: The main isolated fungal spores from the atmosphere of Tabriz district were Penicillium Sp. (36.6%), Cladosporidium Sp. (26.8%) and Aspergillus Sp. (23.6%).

Conclusions: The presence of fungal spores in the atmosphere as a part of air pollution can cause significant problems for human health, particularly in the respiratory tracts.

Keywords

Fungi Spores Fungal Tabriz Aspergillus fumigatus

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background

A large body of evidence has demonstrated that human urbanization has a relationship with the increasing exposure to airborne microorganisms that may result in a variety of adverse health effects, and can particularly lead to allergic symptoms (1-3). The major allergic manifestations include asthma, rhinitis, hypersensitivity pneumonitis, and other diseases such as immunotoxic diseases (e.g. sick building syndrome "SBS") (4).

Respiratory abnormalities, diseases, and symptoms which could be caused by exposure to fungal spores, include asthma and asthma development, exacerbation of asthma, hypersensitivity pneumonitis, wheeze, cough, dyspnea (shortness of breath), nasal and throat symptoms, nose running and respiratory mycoses. In addition to the above diseases and symptoms, rhinosinusitis and sarcoidosis in water-damaged buildings occupants are also drawing more medical attention (5, 6). Moreover, the increase in respiratory allergy parallels an increase in air pollution. The most abundant air pollutants such as sulphur dioxide, nitrogen dioxide, ozone, microbial and fungal microflora (7-9) in urban areas with high levels of vehicle traffic are a remediable issue.

The presence of allergen particles in fungal spores such as ASPF6, ASPF4, ASPF2, Pen ch 18, 1-3beta-D-glucan, Rho m2with peptide fragments such as Lys89, phe91, and Lys90 enumerate allergic segments that irritate immune response, and causes a IgE serum level increase in allergic people. Therefore, fungal microflora can have an important effect on the respiratory health of people (10, 11).

So far the studies on the types and the count of aerial bio-microflora have shown that Aspergillus Sp., Cladosporidium Sp. and Penicillium Sp. have the highest percentage in aerial microflorain city environments (7, 12, 13). In Iran, according to the results of the previous researches, Penicillium Sp., Chrysosporium Sp., Cladosporidium Sp., Candida Sp., Fusarium Sp. and Alternaria Sp. Spores have been postulated as the highest fungal spores in city atmospheres (14-17). Also some studies indicated that there is relationship between the presence of these fungal spores and mycoses on the area in question (18).

2. Objectives

Accordingly, regarding the importance of the subject, we decided to survey the different types of fungal microflora in the atmosphere of Tabriz, and the purpose was to prove the presence of fungal spores and their species in the region’s air.

3. Materials and Methods
3.1. Sample Collection and Culture

Using settle plate (open plate method), sampling was performed from the air in 262 plates containing Sabouraud’s Dextrose Agar (SDA) (Oxiod Ltd., Basingstoke, Hampshire, U.K.) medium during a 12-month period, and from different parts of the city. The triplicate sampling from different locations (northern, southern, western, eastern and also central regions) of the city was carried out on the same days every week during the survey period (Figure 1).

Descriptive Map of Sampling Areas
Figure 1. Descriptive Map of Sampling Areas

The presence and amounts of spores in the air were assessed by exposing a Petri dish containing media to air for 15 minutes. Plates were incubated in 24ºC for at least twenty-one days. During incubation, the plates were reviewed for the growth of fungal colonies once every 24 hours. The total number of fungal colonies counted on the plates was between 1 to 5 colonies (CFUs).

All plates survived for macroscopic and microscopic characteristics. The growth process of fungi in each of the plates was then monitored closely. The suspected fungi with definitive diagnosis by the use of the mentioned methods were then identified by performing slide culture by Riddle method. Regarding the standard protocols, the pathogenic genomic DNAs of A. fumigatus were obtained from patients’ septum, and Bronchoalveolar lavage (BAL) with approved pulmonary aspergillosis according to their clinical symptoms and paraclinical findings.

3.2. Molecular Assay

To compare the isolated pathogenic A. fumigatus from patients with the plates’ isolated A. fumigatus, DNA extraction from both samples were carried out prior to PCR reaction. PCR was performed with the degenerated primers that were solely designed for a highly conserved region of A. fumigatus’ genome, using bioinformatics tools (multiple alignments). The degenerated primers used were as follows:

Primer Sequence

Forward5’ GAY TGG TRG CTI AGY CCI GA 3’

Reveres5’ AAR AAY TGR TTY TCI ATR TAC 3’

PCR reactions (total volume of 100 µl) were carried out with genomic DNAs of both environmental and pathogenic A. fumigatus using the standard protocols (10µl of 10x PCR buffer, 1 µl from each primers, 1 µl dNTP, 0/5 µl A. fumigatus genomic DNA, 1µl Taq DNA polymerase, 85.5µl DSW). PCR reactions with each primer set (Perkin-Elmer, Applied Biosystems, Warrington, Cheshire, U.K), were optimized (catalyzed) by carrying out the reactions with MgCl2 concentrations in the range of 4 to 6 mM (19). The optimal magnesium concentration was found to be 4 mM. The PCR conditions for the above primers are shown below.

3.3. PCR Conditions

3 min 94ºC x1

1 min 94ºC, 2 min 48ºC, 3 min 72ºC x 29

1 min 94ºC, 2 min 48ºC, 7 min 72ºC x1

PCR reaction produced a single product of ca. 1600 bp with both pathogenic and environmental A. fumigatus’ genomic DNAs, which was as predicted based on the genomic sequences (Figure 2).The implemented control reactions with either the individual primers or with no template DNA, did not produce any amplification products.

Figure 2. Gel Electrophoresis of PCR Products Using Degenerate Primers
Gel Electrophoresis of PCR Products Using Degenerate Primers

PCR products were visualized by gel electrophoresis. Lanes 1, 2 and 3 show the results of the PCR in the absence of genomic DNA (lane 1) or with the primer (lanes 2 & 3). Lanes 4 and 5 show the PCR products obtained from the primers, and both used genomic DNAs of environmental and pathogenic A. fumigatus respectively. Lane 6 shows the molecular weight marker, with band sizes (Kb) indicated at the right. The +sign indicates the direction of the positive electrode during electrophoresis.

4. Results

After the determination of fungal genus using macroscopic/microscopic characteristics and counting the number of fungal colonies, the following results were obtained: Due to the percentage of each fungal genus, the most identified fungi were: Penicillium Sp., Cladosporidium Sp. and Aspergillus Sp. respectively as showed in Table 1. Also the PCR reaction products showed that there are strong genomic similarities between the environmental isolated and pathogenic A. fumigatus, which were obtained from the clinical samples of patients with pulmonary aspergillosis.

Table 1. Colony Count and Percentage of the Identified Fungi
FungiPercentageColony Count
Penicillium Sp.39.6104
Cladosporidium Sp.26.870
Aspergillus Sp.23.662
Tichoderma Sp.5.114
Yeast2.84
Chrysosporium Sp.0.72
Fusarium Sp.0.72
Alternaria Sp.0.72
Acremonium Sp.0.72
Total100262
5. Discussion

Fungi can both degrade the organic materials and structures of their colonies, and contribute to the appearance of symptoms and diseases in the inhabitants of contaminated homes, hospitals, factories, etc. (8, 9). Citizens in city environments are not only at risk of harm to their health through environmental degradation as a result of the worsening air pollution problems such as fungal spores, but are also constantly threatened by emerging and recurring asthma, rhinitis, bronchopulmonary disorders, mycoses and hypersensitivity pneumonitis epidemics (2, 3).

It is notable that fungal spores are a significant component of bioaerosol, and are also considered to act as a marker of the level of atmospheric bio-pollution. Therefore, better understanding of this phenomenon demands a detailed survey of the airborne particles. Some studies have emphasized that fungal spores’ concentration can be a scientific indicator of indoor air quality, and that it is necessary to deepen the studies of indoor atmospheres in order to promote the air quality, the health/hygiene, and a better consideration of the biology of indoor fungi (8, 9).

Tabriz (metropolis in the northwest of Iran), with a high industrialization pace and population growth, and its consequent closeness to the hypothetical epicenters of disease transmission, offers a unique opportunity on testing environmental air contamination with fungal spores. According to the obtained results, various species of Cladosporidium Sp., Fusarium Sp. and Aspergillus SP., after Penicillium Sp., contain the highest percentage of fungal spores in the studied area. Also in some other studies about fungal air pollution, Aspergillus Sp. and Penicillium Sp. were the most abundant fungal spores’ type with a yearly mean percentage that represented 65% of the total, followed by Cladosporium Sp. (12).

According to the previous studies on this subject, Penicillium Sp., Chrysosporium Sp., Cladosporidium Sp., Candida Sp., Alternaria Sp.Fusarium Sp., Ulocladium Sp., Geotrichum Sp., and Zygomycota (Mucor & Rhizopus spp.) were the most common fungal air flora in various regions of Iran. The studies of fungal air spores in different cities of Iran including Ahvaz (20, 21) Isfahan (14, 22), Ardestan (23), Shahrekord (24), Qeshm island (25), Kerman (26), Qom (27), Qazvin (28), Gonabad (29), Babol city and Babolkenar forest (30), Yazd (31), Semnan (32), Sari (33), Urmia (34), Tabriz (18) and Lorestan province (35) have mainly shown similar results, as mentioned above.

Some studies also indicate a relationship between the presence of these fungal spores and mycoses in the studied areas (14, 21, 32, 35). Similar studies in the neighboring (or close) countries to Iran such as Turkey (36), Pakistan (37) and India (38) also demonstrated the presence of the same fungi in their atmosphere with some minor exceptions. For example in Turkey (36) Chrysosporium sp., Conidiobolus sp., Cladothecium sp., and in Karachi (Pakistan) (37) Periconia sp., Curvularia sp., and Stemphylium sp., have been isolated in addition to the usual fungal air flora. In Kolkata (India), more than 50 fungal taxa of airborne fungal spores were isolated from the air, among which at least 15 taxa were considered as allergenic fungi (17).

Fungal spores inhalation or other kinds of exposure to these fungi can cause respiratory diseases or illnesses including asthma, cutaneous and visceral mycoses such as fungal keratitis, otomycosis, and other various clinical symptoms of aspergillosis and penicilliosis (17, 18). Among the above mycoses, pulmonary infection with Aspergillus and Penicillium sp., (as two etiologic agents of life threatening infections) is very prevalent, particularly amongst immunocompromised patients as well as chemotherapy treated patients, radiotherapy, HIV+, and bone marrow recipients (38-42).

Also, in this study, a comparison between the environmental and clinical samples of A. fumigatususing molecular assay showed a similarity among both samples. This finding could be considered as evidence on the source of airborne mycoses due to inhalation of environmental fungal spores (41). Exposure to these kinds of environmental fungal spores could lead to deep mycoses, such as different types of pulmonary aspergillosis (IA, ABPA, CBPA, aspergilloma) and sinusitis, particularly among immunocompromised patients with serious consequences (15, 43).

The results of this study can be beneficial for the management of public health surveillance particularly on asthma, rhinitis, bronchopulmonary disorders and cutaneous mycoses in the region. It may attract the attention of the regional health systems managers towards the several risks to the health of the region’s population when considering environmental protection and management in the context of economics, urban, and infra structural developments. This is the first study in Tabriz area to examine the fungal air pollution from an environmental health perspective. However, more studies are needed to further document its efficacy, and also perform a cost-benefit analysis of these services.

Acknowledgements
Footnotes
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