Jundishapur Journal of Microbiology

Published by: Kowsar

Identification of Cutaneous Leishmaniasis Agents in Four Geographical Regions of Khuzestan Province Using Nested PCR

Sharif Maraghi 1 , * , Omid Mardanshah 1 , Abdollah Rafiei 2 , Alireza Samarbafzadeh 3 and Babak Vazirianzadeh 4
Authors Information
1 Department of Parasitology and Mycology, Arvand International Division, Infectious and Tropical Diseases Research Center, Thalassemia and Haemoglobinopathy Research Center Ahvaz, IR Iran
2 Department of Parasitology, School of Medicine, Infectious and Tropical Diseases Research Center, Ahvaz, IR Iran
3 Department of Virology, School of Medicine, Ahvaz, IR Iran
4 Department of Entomology, School of Health, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: June 01, 2013, 6 (4); e4866
  • Published Online: June 10, 2013
  • Article Type: Research Article
  • Received: April 30, 2012
  • Revised: June 20, 2012
  • Accepted: July 8, 2012
  • DOI: 10.5812/jjm.4866

To Cite: Maraghi S, Mardanshah O, Rafiei A, Samarbafzadeh A, Vazirianzadeh B. Identification of Cutaneous Leishmaniasis Agents in Four Geographical Regions of Khuzestan Province Using Nested PCR, Jundishapur J Microbiol. 2013 ; 6(4):e4866. doi: 10.5812/jjm.4866.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Ashford RW. The leishmaniases as emerging and reemerging zoonoses. Int J Parasitol. 2000; 30(12-13): 1269-81[PubMed]
  • 2. Silveira FT, Ishikawa EA, De Souza AA, Lainson R. An outbreak of cutaneous leishmaniasis among soldiers in Belem, Para State, Brazil, caused by Leishmania (Viannia) lindenbergi n. sp. A new leishmanial parasite of man in the Amazon region. Parasite. 2002; 9(1): 43-50[PubMed]
  • 3. Desjeux P. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg. 2001; 95(3): 239-43[PubMed]
  • 4. Herwaldt BL. Leishmaniasis. Lancet. 1999; 354(9185): 1191-9[DOI][PubMed]
  • 5. Faber WR, Oskam L, van Gool T, Kroon NC, Knegt-Junk KJ, Hofwegen H, et al. Value of diagnostic techniques for cutaneous leishmaniasis. J Am Acad Dermatol. 2003; 49(1): 70-4[DOI][PubMed]
  • 6. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travellers. J Antimicrob Chemother. 2004; 53(2): 158-66[DOI][PubMed]
  • 7. Chargui N, Bastien P, Kallel K, Haouas N, Akrout FM, Masmoudi A, et al. Usefulness of PCR in the diagnosis of cutaneous leishmaniasis in Tunisia. Trans R Soc Trop Med Hyg. 2005; 99(10): 762-8[DOI][PubMed]
  • 8. Safaei A, Motazedian MH, Vasei M. Polymerase chain reaction for diagnosis of cutaneous leishmaniasis in histologically positive, suspicious and negative skin biopsies. Dermatology. 2002; 205(1): 18-24[PubMed]
  • 9. Noyes HA, Reyburn H, Bailey JW, Smith D. A nested-PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan. J Clin Microbiol. 1998; 36(10): 2877-81[PubMed]
  • 10. Karamian M, Motazedian MH, Fakhar M, Pakshir K, Jowkar F, Rezanezhad H. Atypical presentation of Old-World cutaneous leishmaniasis, diagnosis and species identification by PCR. J Eur Acad Dermatol Venereol. 2008; 22(8): 958-62[DOI][PubMed]
  • 11. Motazedian MH, Mehrabani D, Oryan A, Asgari Q, Karamian M, Kalantari M. Life cycle of cutaneous leishmaniasis in Larestan, southern Iran. Iran J Clin Infect Dis. 2006; 1(3)
  • 12. Lachaud L, Dereure J, Chabbert E, Reynes J, Mauboussin JM, Oziol E, et al. Optimized PCR using patient blood samples for diagnosis and follow-up of visceral Leishmaniasis, with special reference to AIDS patients. J Clin Microbiol. 2000; 38(1): 236-40[PubMed]
  • 13. Mauricio IL, Stothard JR, Miles MA. Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon. Parasitology. 2004; 128: 263-7[PubMed]
  • 14. Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 2003; 47(1): 349-58[PubMed]
  • 15. Leishmaniasis disease information.
  • 16. el Tai NO, Osman OF, el Fari M, Presber W, Schonian G. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg. 2000; 94(5): 575-9[PubMed]
  • 17. Maraghi S, Samarbafzadeh AR, Sarlak AA, Ghasemian M, Vazirianzadeh B. Identification of Cutaneous Leishmaniasis Agents by Nested Po-lymerase Chain Reaction (Nested-PCR) in Shush City, Khuzestan Province, Iran. Iran J Parasitol. 2007; 2(3)
  • 18. Ghasemian M, Maraghi S, Samarbafzadeh AR, Jelowdar A, Kalantari M. The PCR-based detection and identification of the parasites causing human cutaneous leishmaniasis in the Iranian city of Ahvaz. Ann Trop Med Parasitol. 2011; 105(3): 209-15[DOI][PubMed]
  • 19. Saki J, Meamar A, Oormazdi H, Akhlaghi L, Maraghi S, Mohebali M, et al. Mini-exon genotyping of leishmania species in khuzestan province, southwest iran. Iran J Parasitol. 2010; 5(1): 25-34[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments