Jundishapur Journal of Microbiology

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Comparison of Phenotypic Tests and PCR to Detect Candida Albicans From Vaginal Specimens (Tabriz, 2009-2010)

Abdolhassan Kazemi 1 , Mehraban Falahati 2 , * , Ashraf Hajipoor 1 , Abbasali Jafari 3 and Mohammad Asghar zadeh 4
Authors Information
1 Tropical and Infectious Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Medical Mycology, School of Medicine, Yazd University of Medical Sciences, Yazd, IR Iran
4 Tropical and Infectious Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: March 01, 2013, 6 (2); e4734
  • Published Online: March 2, 2013
  • Article Type: Research Article
  • Received: March 18, 2012
  • Revised: May 7, 2012
  • Accepted: May 22, 2012
  • DOI: 10.5812/jjm.4734

To Cite: Kazemi A, Falahati M, Hajipoor A, Jafari A, Asghar zadeh M. Comparison of Phenotypic Tests and PCR to Detect Candida Albicans From Vaginal Specimens (Tabriz, 2009-2010), Jundishapur J Microbiol. 2013 ; 6(2):e4734. doi: 10.5812/jjm.4734.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Ahmad S, Khan Z, Mustafa AS, Khan ZU. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol. 2002; 40(7): 2483-9[DOI][PubMed]
  • 2. Odds FC, Webster CE, Riley VC, Fisk PG. Epidemiology of vaginal Candida infection: significance of numbers of vaginal yeasts and their biotypes. Eur J Obstet Gynecol Reprod Biol. 1987; 25(1): 53-66[DOI]
  • 3. Sobel JD. Candida vulvovaginitis. Semin Dermatol. 1996; 15(1): 17-28[DOI]
  • 4. Consolaro MEL, Albertoni TA, Yoshida CS, Mazucheli J, Peralta RM, Svidzinski TIE. Correlation of Candida species and symptoms among patients with vulvovaginal candidiasis in Maringá, Paraná, Brazil. Rev Iberoam Micol. 2004; 21(4): 202-5
  • 5. Sobel JD, Faro S, Force RW, Foxman B, Ledger WJ, Nyirjesy PR, et al. Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations. Am J Obstet Gynecol. 1998; 178(2): 203-11[DOI]
  • 6. Bader MS, Lai SM, Kumar V, Hinthorn D. Candidemia in patients with diabetes mellitus: epidemiology and predictors of mortality. Scand J Infect Dis. 2004; 36(11-12): 860-4[DOI]
  • 7. Lo HJ, Kohler JR, DiDomenico B, Loebenberg D, Cacciapuoti A, Fink GR. Nonfilamentous C. albicans mutants are avirulent. Cell. 1997; 90(5): 939-49[DOI]
  • 8. Romani L, Mencacci A, Grohmann U, Mocci S, Mosci P, Puccetti P. Neutralizing antibody to interleukin 4 induces systemic protection and T helper type 1-associated immunity in murine candidiasis. J Exp Med. 1992; 176(1): 19-25[DOI][PubMed]
  • 9. Dan M, Segal R, Marder V, Leibovitz A. Candida colonization of the vagina in elderly residents of a long-term-care hospital. Eur J Clin Microbiol Infect Dis. 2006; 25(6): 394-6[DOI][PubMed]
  • 10. Goswami D, Goswami R, Banerjee U, Dadhwal V, Miglani S, Lattif AA, et al. Pattern of Candida species isolated from patients with diabetes mellitus and vulvovaginal candidiasis and their response to single dose oral fluconazole therapy. J Infect. 2006; 52(2): 111-7[DOI][PubMed]
  • 11. Kirkpatrick WR, Revankar SG, McAtee RK, Lopez-Ribot JL, Fothergill AW, McCarthy DI. Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar candida screening and susceptibility testing of isolates. J Clin Microbiol. 1998; 36(10): 3007-12[PubMed]
  • 12. Ray D, Goswami R, Banerjee U, Dadhwal V, Goswami D, Mandal P, et al. Prevalence of Candida glabrata and its response to boric acid vaginal suppositories in comparison with oral fluconazole in patients with diabetes and vulvovaginal candidiasis. Diabetes Care. 2007; 30(2): 312-7[DOI][PubMed]
  • 13. Sweet CE, Kaufman L. Application of agglutinins for the rapid and accurate identification of medically important Candida species. Appl Microbiol. 1970; 19(5): 830-6[PubMed]
  • 14. Trama JP, Adelson ME, Raphaelli I, Stemmer SM, Mordechai E. Detection of Candida species in vaginal samples in a clinical laboratory setting. Infect Dis Obstet Gynecol. 2005; 13(2): 63-7[DOI][PubMed]
  • 15. Abu-Elteen KH, Abdul Malek AM, Abdul Wahid NA. Prevalence and susceptibility of vaginal yeast isolates in Jordan. Mycoses. 1997; 40(5-6): 179-85[DOI][PubMed]
  • 16. Ahmad A, Khan AU. Prevalence of Candida species and potential risk factors for vulvovaginal candidiasis in Aligarh, India. Eur J Obstet Gynecol Reprod Biol. 2009; 144(1): 68-71[DOI][PubMed]
  • 17. Spinillo A, Capuzzo E, Gulminetti R, Marone P, Colonna L, Piazzi G. Prevalence of and risk factors for fungal vaginitis caused by non-albicans species. Am J Obstet Gynecol. 1997; 176(1 Pt 1): 138-41[DOI]
  • 18. Ainscough S, Kibbler CC. An evaluation of the cost-effectiveness of using CHROMagar for yeast identification in a routine microbiology laboratory. J Med Microbiol. 1998; 47(7): 623-8[DOI]
  • 19. Hoppe JE, Frey P. Evaluation of six commercial tests and the germ-tube test for presumptive identification of Candida albicans. Eur J Clin Microbiol Infect Dis. 1999; 18(3): 188-91[DOI]
  • 20. Merlino J, Tambosis E, Veal D. Chromogenic tube test for presumptive identification or confirmation of isolates as Candida albicans. J Clin Microbiol. 1998; 36(4): 1157-9[PubMed]
  • 21. Yucesoy M, Esen N, Yulug N. Use of chromogenic tube and methyl blue-sabouraud agar for the identification of Candida albicans strains. Kobe J Med Sci. 2001; 47(4): 161-7[PubMed]
  • 22. Quindos G, San Millan R, Robert R, Bernard C, Ponton J. Evaluation of bichro-latex albicans, a new method for rapid identification of Candida albicans. J Clin Microbiol. 1997; 35(5): 1263-5
  • 23. Foongladda S, Haouharn P, Sakulmaiwatana P, Chaiprasert A. Comparative evaluation of Candi Select test and conventional methods for identification of Candida albicans in routine clinical isolates. Mycoses. 2002; 45(3-4): 75-8[DOI][PubMed]
  • 24. Giraldo P, von Nowaskonski A, Gomes FA, Linhares I, Neves NA, Witkin SS. Vaginal colonization by Candida in asymptomatic women with and without a history of recurrent vulvovaginal candidiasis. Obstet Gynecol. 2000; 95(3): 413-6[DOI]
  • 25. Coleman DC, Sullivan DJ, Bennett DE, Moran GP, Barry HJ, Shanley DB. Candidiasis: the emergence of a novel species, Candida dubliniensis. AIDS. 1997; 11(5): 557-67[DOI][PubMed]
  • 26. Atkins SD, Clark IM. Fungal molecular diagnostics: a mini review. J Appl Genet. 2004; 45(1): 3-15[PubMed]
  • 27. Mahnss B, Stehr F, Schafer W, Neuber K. Comparison of standard phenotypic assays with a PCR method to discriminate Candida albicans and C dubliniensis. Mycoses. 2005; 48(1): 55-61[DOI][PubMed]
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