Jundishapur Journal of Microbiology

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Frequency of Helicobacter pylori hopQI, hopQII and sabA Genes Among Iranian Patients with Gastroduodenal Diseases

Zahra Sedarat 1 , 2 , Reza Khashei 3 , Hedayatollah Shirzad 1 , Nader Bagheri 4 , Marzieh Sadeghiani 1 , Heshmat Shahi 1 and Behnam Zamanzad 1 , *
Authors Information
1 Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Basic Sciences of Center, Shahid Dooran Pardis, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Article information
  • Jundishapur Journal of Microbiology: August 2018, 11 (8); e56017
  • Published Online: August 7, 2018
  • Article Type: Research Article
  • Received: June 23, 2018
  • Revised: July 3, 2018
  • Accepted: July 30, 2018
  • DOI: 10.5812/jjm.56017

To Cite: Sedarat Z , Khashei R, Shirzad H, Bagheri N , Sadeghiani M , et al. Frequency of Helicobacter pylori hopQI, hopQII and sabA Genes Among Iranian Patients with Gastroduodenal Diseases, Jundishapur J Microbiol. 2018 ; 11(8):e56017. doi: 10.5812/jjm.56017.

Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Oleastro M, Menard A. The Role of Helicobacter pylori Outer Membrane Proteins in Adherence and Pathogenesis. Biology (Basel). 2013;2(3):1110-34. doi: 10.3390/biology2031110. [PubMed: 24833057]. [PubMed Central: PMC3960876].
  • 2. Haddadi MH, Bazargani A, Khashei R, Fattahi MR, Bagheri Lankarani K, Moini M, et al. Different distribution of Helicobacter pylori EPIYA- cagA motifs and dupA genes in the upper gastrointestinal diseases and correlation with clinical outcomes in iranian patients. Gastroenterol Hepatol Bed Bench. 2015;8(Suppl 1):S37-46. [PubMed: 26171136]. [PubMed Central: PMC4495425].
  • 3. Ilver D, Arnqvist A, Ogren J, Frick IM, Kersulyte D, Incecik ET, et al. Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science. 1998;279(5349):373-7. doi: 10.1126/science.279.5349.373. [PubMed: 9430586].
  • 4. Mahdavi J, Sonden B, Hurtig M, Olfat FO, Forsberg L, Roche N, et al. Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation. Science. 2002;297(5581):573-8. doi: 10.1126/science.1069076. [PubMed: 12142529]. [PubMed Central: PMC2570540].
  • 5. Yamaoka Y, Kwon DH, Graham DY. A M(r) 34,000 proinflammatory outer membrane protein (oipA) of Helicobacter pylori. Proc Natl Acad Sci U S A. 2000;97(13):7533-8. doi: 10.1073/pnas.130079797. [PubMed: 10852959]. [PubMed Central: PMC16580].
  • 6. Odenbreit S, Till M, Hofreuter D, Faller G, Haas R. Genetic and functional characterization of the alpAB gene locus essential for the adhesion of Helicobacter pylori to human gastric tissue. Mol Microbiol. 1999;31(5):1537-48. doi: 10.1046/j.1365-2958.1999.01300.x. [PubMed: 10200971].
  • 7. Cao P, Cover TL. Two different families of hopQ alleles in Helicobacter pylori. J Clin Microbiol. 2002;40(12):4504-11. doi: 10.1128/JCM.40.12.4504-4511.2002. [PubMed: 12454143]. [PubMed Central: PMC154617].
  • 8. Loh JT, Torres VJ, Algood HM, McClain MS, Cover TL. Helicobacter pylori HopQ outer membrane protein attenuates bacterial adherence to gastric epithelial cells. FEMS Microbiol Lett. 2008;289(1):53-8. doi: 10.1111/j.1574-6968.2008.01368.x. [PubMed: 19065710]. [PubMed Central: PMC2651568].
  • 9. Ohno T, Sugimoto M, Nagashima A, Ogiwara H, Vilaichone RK, Mahachai V, et al. Relationship between Helicobacter pylori hopQ genotype and clinical outcome in Asian and Western populations. J Gastroenterol Hepatol. 2009;24(3):462-8. doi: 10.1111/j.1440-1746.2008.05762.x. [PubMed: 19226380]. [PubMed Central: PMC3128252].
  • 10. Cao P, Lee KJ, Blaser MJ, Cover TL. Analysis of hopQ alleles in East Asian and Western strains of Helicobacter pylori. FEMS Microbiol Lett. 2005;251(1):37-43. doi: 10.1016/j.femsle.2005.07.023. [PubMed: 16102915].
  • 11. Sicinschi LA, Correa P, Bravo LE, Peek RM Jr, Wilson KT, Loh JT, et al. Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children. Helicobacter. 2012;17(2):96-106. doi: 10.1111/j.1523-5378.2011.00919.x. [PubMed: 22404439]. [PubMed Central: PMC3305281].
  • 12. Chiarini A, Cala C, Bonura C, Gullo A, Giuliana G, Peralta S, et al. Prevalence of virulence-associated genotypes of Helicobacter pylori and correlation with severity of gastric pathology in patients from western Sicily, Italy. Eur J Clin Microbiol Infect Dis. 2009;28(5):437-46. doi: 10.1007/s10096-008-0644-x. [PubMed: 18958508].
  • 13. Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006;19(3):449-90. doi: 10.1128/CMR.00054-05. [PubMed: 16847081]. [PubMed Central: PMC1539101].
  • 14. Yamaoka Y. Increasing evidence of the role of Helicobacter pylori SabA in the pathogenesis of gastroduodenal disease. J Infect Dev Ctries. 2008;2(3):174-81. doi: 10.3855/jidc.259. [PubMed: 19738347]. [PubMed Central: PMC2833341].
  • 15. Skindersoe ME, Rasmussen L, Andersen LP, Krogfelt KA. A Novel Assay for Easy and Rapid Quantification of Helicobacter pylori Adhesion. Helicobacter. 2015;20(3):199-205. doi: 10.1111/hel.12191. [PubMed: 25603836].
  • 16. Salimzadeh L, Bagheri N, Zamanzad B, Azadegan-Dehkordi F, Rahimian G, Hashemzadeh-Chaleshtori M, et al. Frequency of virulence factors in Helicobacter pylori-infected patients with gastritis. Microb Pathog. 2015;80:67-72. doi: 10.1016/j.micpath.2015.01.008. [PubMed: 25656240].
  • 17. Bagheri N, Taghikhani A, Rahimian G, Salimzadeh L, Azadegan Dehkordi F, Zandi F, et al. Association between virulence factors of helicobacter pylori and gastric mucosal interleukin-18 mRNA expression in dyspeptic patients. Microb Pathog. 2013;65:7-13. doi: 10.1016/j.micpath.2013.08.005. [PubMed: 24036181].
  • 18. Shahi H, Bahreini R, Reiisi S, Sadeghiani M. Association between H. pylori babA virulence factor with clinical outcome and ABO blood groups. J Pure Appl Microbiol. 2015;9:285-90.
  • 19. Nishioka M, Takeuchi H, Con SA, Uehara Y, Nishimori I, Okumiya T, et al. The mechanical binding strengths of Helicobacter pylori BabA and SabA adhesins using an adhesion binding assay-ELISA, and its clinical relevance in Japan. Microbiol Immunol. 2010;54(8):442-51. doi: 10.1111/j.1348-0421.2010.00237.x. [PubMed: 20646208].
  • 20. Talebi Bezmin Abadi A, Mohabbati Mobarez A. High Prevalence of Helicobacter pylori hopQ II Genotype Isolated from Iranian Patients with Gastroduodenal Disorders. J Pathog. 2014;2014:842469. doi: 10.1155/2014/842469. [PubMed: 24672729]. [PubMed Central: PMC3941210].
  • 21. Ortiz-Princz D, Daoud G, Salgado-Sabel A, Cavazza ME. Helicobacter pylori infection in children: should it be carefully assessed? Eur Rev Med Pharmacol Sci. 2016;20(9):1798-813. [PubMed: 27212173].
  • 22. Shiota S, Suzuki R, Yamaoka Y. The significance of virulence factors in Helicobacter pylori. J Dig Dis. 2013;14(7):341-9. doi: 10.1111/1751-2980.12054. [PubMed: 23452293]. [PubMed Central: PMC3721066].
  • 23. Dara M, Khashei R, Dehghani B. High frequency of hopQ genotypes among Iranian Helicobacter pylori clinical isolates. Infez Med. 2017;25(2):123-6. [PubMed: 28603230].
  • 24. Kazemi E, Kahrizi D, Moradi MT, Sohrabi M, Amini S, Mousavi SA, et al. Association between Helicobacter pylori hopQI genotypes and human gastric cancer risk. Cell Mol Biol (Noisy-le-grand). 2016;62(1):6-9. [PubMed: 26828979].
  • 25. Posselt G, Backert S, Wessler S. The functional interplay of Helicobacter pylori factors with gastric epithelial cells induces a multi-step process in pathogenesis. Cell Commun Signal. 2013;11:77. doi: 10.1186/1478-811X-11-77. [PubMed: 24099599]. [PubMed Central: PMC3851490].
  • 26. Sheu BS, Odenbreit S, Hung KH, Liu CP, Sheu SM, Yang HB, et al. Interaction between host gastric Sialyl-Lewis X and H. pylori SabA enhances H. pylori density in patients lacking gastric Lewis B antigen. Am J Gastroenterol. 2006;101(1):36-44. doi: 10.1111/j.1572-0241.2006.00358.x. [PubMed: 16405531].
  • 27. Lehours P, Menard A, Dupouy S, Bergey B, Richy F, Zerbib F, et al. Evaluation of the association of nine Helicobacter pylori virulence factors with strains involved in low-grade gastric mucosa-associated lymphoid tissue lymphoma. Infect Immun. 2004;72(2):880-8. doi: 10.1128/IAI.72.2.880-888.2004. [PubMed: 14742532]. [PubMed Central: PMC321584].
  • 28. de Jonge R, Pot RG, Loffeld RJ, van Vliet AH, Kuipers EJ, Kusters JG. The functional status of the Helicobacter pylori sabB adhesin gene as a putative marker for disease outcome. Helicobacter. 2004;9(2):158-64. doi: 10.1111/j.1083-4389.2004.00213.x. [PubMed: 15068418].
  • 29. Yanai A, Maeda S, Hikiba Y, Shibata W, Ohmae T, Hirata Y, et al. Clinical relevance of Helicobacter pylori sabA genotype in Japanese clinical isolates. J Gastroenterol Hepatol. 2007;22(12):2228-32. doi: 10.1111/j.1440-1746.2007.04831.x. [PubMed: 18031386].
  • 30. Shao L, Takeda H, Fukui T, Mabe K, Han J, Kawata S, et al. Genetic diversity of the Helicobacter pylori sialic acid-binding adhesin (sabA) gene. Biosci Trends. 2010;4(5):249-53. [PubMed: 21068478].
  • 31. Yamaoka Y, Ojo O, Fujimoto S, Odenbreit S, Haas R, Gutierrez O, et al. Helicobacter pylori outer membrane proteins and gastroduodenal disease. Gut. 2006;55(6):775-81. doi: 10.1136/gut.2005.083014.
  • 32. Pakbaz Z, Shirazi MH, Ranjbar R, Pourmand MR, Khalifeh Gholi M, Aliramezani A, et al. Frequency of sabA Gene in Helicobacter pylori Strains Isolated From Patients in Tehran, Iran. Iran Red Crescent Med J. 2013;15(9):767-70. doi: 10.5812/ircmj.5044. [PubMed: 24616782]. [PubMed Central: PMC3929807].
  • 33. Yadegar A, Alebouyeh M, Zali MR. Analysis of the intactness of Helicobacter pylori cag pathogenicity island in Iranian strains by a new PCR-based strategy and its relationship with virulence genotypes and EPIYA motifs. Infect Genet Evol. 2015;35:19-26. doi: 10.1016/j.meegid.2015.07.026. [PubMed: 26205689].
  • 34. Sohrabi M, Khashei R, Alizadeh M, Asl MH, Nejati MA, Dara M, et al. Low Rate of babA2 Genotype among Iranian Helicobacter pylori Clinical Isolates. J Clin Diagn Res. 2017;11(7):DC32-6. doi: 10.7860/JCDR/2017/24810.10277. [PubMed: 28892894]. [PubMed Central: PMC5583947].
  • 35. Ben Mansour K, Fendri C, Zribi M, Masmoudi A, Labbene M, Fillali A, et al. Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients. Ann Clin Microbiol Antimicrob. 2010;9:10. doi: 10.1186/1476-0711-9-10. [PubMed: 20302630]. [PubMed Central: PMC2855517].

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