Jundishapur Journal of Microbiology

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Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran

Mansour Amin 1 , 2 , Mojtaba Soltan Abadi 1 , Tahereh Navidifar 1 , * , Mehdi Torabizadeh 3 and Seyed Mohammad Alavi 2
Authors Information
1 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Infectious and Tropical Diseases Research Center,Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Abouzar Children’s Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Article information
  • Jundishapur Journal of Microbiology: February 2018, 11 (2); e55554
  • Published Online: January 21, 2018
  • Article Type: Research Article
  • Received: June 23, 2017
  • Revised: November 30, 2017
  • Accepted: December 18, 2017
  • DOI: 10.5812/jjm.55554

To Cite: Amin M, Soltan Abadi M, Navidifar T, Torabizadeh M, Alavi S M. Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran, Jundishapur J Microbiol. 2018 ; 11(2):e55554. doi: 10.5812/jjm.55554.

Abstract
Copyright © 2018, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusion
Acknowledgements
Footnotes
References
  • 1. Chen FQ, Yang YZ, Yu LL, Bi CB. Prevalence of Mycoplasma pneumoniae: A cause for community-acquired infection among pediatric populaztion. Nigerian Journal of Clinical Practice. 2015;18(3):354. doi: 10.4103/1119-3077.153247.
  • 2. Saraya T. The History of Mycoplasma pneumoniae Pneumonia. Front Microbiol. 2016;7. doi: 10.3389/fmicb.2016.00364.
  • 3. Kashyap S, Sarkar M. Mycoplasma pneumonia: Clinical features and management. Lung India. 2010;27(2):75-85. doi: 10.4103/0970-2113.63611. [PubMed: 20616940].
  • 4. Saraya T. Mycoplasma pneumoniae infection: Basics. J Gen Fam Med. 2017;18(3):118-25. doi: 10.1002/jgf2.15.
  • 5. Zhou D, del Valle-Mendoza J, Orellana-Peralta F, Marcelo-Rodríguez A, Verne E, Esquivel-Vizcarra M, et al. High Prevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in Children with Acute Respiratory Infections from Lima, Peru. Plos One. 2017;12(1). e0170787. doi: 10.1371/journal.pone.0170787.
  • 6. Lee E, Cho HJ, Hong SJ, Lee J, Sung H, Yu J. Prevalence and clinical manifestations of macrolide resistant Mycoplasma pneumoniae pneumonia in Korean children. Korean J Pediatr. 2017;60(5):151. doi: 10.3345/kjp.2017.60.5.151.
  • 7. Zhang L, Zong ZY, Liu YB, Ye H, Lv XJ. PCR versus serology for diagnosing Mycoplasma pneumoniae infection: a systematic review & meta-analysis. Indian J Med Res. 2011;134:270-80. [PubMed: 21985809].
  • 8. Thurman KA, Walter ND, Schwartz SB, Mitchell SL, Dillon MT, Baughman AL, et al. Comparison of laboratory diagnostic procedures for detection of Mycoplasma pneumoniae in community outbreaks. Clin Infect Dis. 2009;48(9):1244-9. doi: 10.1086/597775. [PubMed: 19331586].
  • 9. Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev. 2008;32(6):956-73. doi: 10.1111/j.1574-6976.2008.00129.x. [PubMed: 18754792].
  • 10. Loens K, Goossens H, Ieven M. Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods. Eur J Clin Microbiol Infect Dis. 2010;29(9):1055-69. doi: 10.1007/s10096-010-0975-2. [PubMed: 20526788].
  • 11. Pitcher D, Chalker VJ, Sheppard C, George RC, Harrison TG. Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control. J Med Microbiol. 2006;55(Pt 2):149-55. doi: 10.1099/jmm.0.46281-0. [PubMed: 16434706].
  • 12. Cherry J, Mundi J, Shapiro N. Rhinosinusitis. Feigin and Cherry’s Textbook of Pediatric Infectious Diseases, 7th ed., edited by JD Cherry, GJ Harrison, SL Kaplan, WJ Steinbach, and PJ Hotez. Philadelphia: Elsevier Saunders. 2014:193-203.
  • 13. Kashyap B, Kumar S, Sethi GR, Das BC, Saigal SR. Comparison of PCR, culture & serological tests for the diagnosis of Mycoplasma pneumoniae in community-acquired lower respiratory tract infections in children. Indian J Med Res. 2008;128(2):134-9. [PubMed: 19001676].
  • 14. Yoon IA, Hong KB, Lee HJ, Yun KW, Park JY, Choi YH, et al. Radiologic findings as a determinant and no effect of macrolide resistance on clinical course of Mycoplasma pneumoniae pneumonia. BMC Infect Dis. 2017;17(1):402. doi: 10.1186/s12879-017-2500-z. [PubMed: 28592263].
  • 15. Defilippi A, Silvestri M, Tacchella A, Giacchino R, Melioli G, Di Marco E, et al. Epidemiology and clinical features of Mycoplasma pneumoniae infection in children. Respir Med. 2008;102(12):1762-8. doi: 10.1016/j.rmed.2008.06.022. [PubMed: 18703327].
  • 16. Almasri M, Diza E, Papa A, Eboriadou M, Souliou E. Mycoplasma pneumoniae respiratory tract infections among Greek children. Hippokratia. 2011;15(2):147-52. [PubMed: 22110297].
  • 17. Sidal M, Kilic A, Unuvar E, Oguz F, Onel M, Agacfidan A, et al. Frequency of Chlamydia pneumoniae and Mycoplasma pneumoniae infections in children. J Trop Pediatr. 2007;53(4):225-31. doi: 10.1093/tropej/fmm003. [PubMed: 17517817].
  • 18. Spuesens EB, Meijer A, Bierschenk D, Hoogenboezem T, Donker GA, Hartwig NG, et al. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae in respiratory specimens collected between 1997 and 2008 in The Netherlands. J Clin Microbiol. 2012;50(6):1999-2004. doi: 10.1128/JCM.00400-12. [PubMed: 22495561].
  • 19. Nilsson AC, Bjorkman P, Persson K. Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection. BMC Microbiol. 2008;8:93. doi: 10.1186/1471-2180-8-93. [PubMed: 18547431].
  • 20. Morozumi M, Iwata S, Hasegawa K, Chiba N, Takayanagi R, Matsubara K, et al. Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia. Antimicrob Agents Chemother. 2008;52(1):348-50. doi: 10.1128/AAC.00779-07. [PubMed: 17954691].
  • 21. Ghotaslou R, Sharifi S, Akhi MT, Soroush MH. Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran. Turk J Med Sci. 2013;43(4):521-4.
  • 22. Medjo B, Atanaskovic-Markovic M, Radic S, Nikolic D, Lukac M, Djukic S. Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia in children: clinical features and laboratory diagnosis. Ital J Pediatr. 2014;40:104. doi: 10.1186/s13052-014-0104-4. [PubMed: 25518734].
  • 23. She RC, Thurber A, Hymas WC, Stevenson J, Langer J, Litwin CM, et al. Limited utility of culture for Mycoplasma pneumoniae and Chlamydophila pneumoniae for diagnosis of respiratory tract infections. J Clin Microbiol. 2010;48(9):3380-2. doi: 10.1128/JCM.00321-10. [PubMed: 20610673].
  • 24. Martinez MA, Ruiz M, Zunino E, Luchsinger V, Avendano LF. Detection of Mycoplasma pneumoniae in adult community-acquired pneumonia by PCR and serology. J Med Microbiol. 2008;57(Pt 12):1491-5. doi: 10.1099/jmm.0.2008/003814-0. [PubMed: 19018018].
  • 25. Chang HY, Chang LY, Shao PL, Lee PI, Chen JM, Lee CY, et al. Comparison of real-time polymerase chain reaction and serological tests for the confirmation of Mycoplasma pneumoniae infection in children with clinical diagnosis of atypical pneumonia. J Microbiol Immunol Infect. 2014;47(2):137-44. doi: 10.1016/j.jmii.2013.03.015. [PubMed: 23726653].
  • 26. Morozumi M, Nakayama E, Iwata S, Aoki Y, Hasegawa K, Kobayashi R, et al. Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol. 2006;44(4):1440-6. doi: 10.1128/JCM.44.4.1440-1446.2006. [PubMed: 16597874].
  • 27. Qu J, Gu L, Wu J, Dong J, Pu Z, Gao Y, et al. Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia. BMC Infect Dis. 2013;13:172. doi: 10.1186/1471-2334-13-172. [PubMed: 23578215].

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