Jundishapur Journal of Microbiology

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A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa

Yang Guojun 1 , He Yuping 2 , Jiang Yan 1 , Lin Kaichun 1 , 2 and Xia Haiyang 1 , 2 , 3 , *
Authors Information
1 Hubei Nature’s Favor Biotechnology, Hanchuan, Hubei, People’s Republic of China
2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People’s Republic of China
3 Key laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai, People’s Republic of China
Article information
  • Jundishapur Journal of Microbiology: June 01, 2016, 9 (6); e16765
  • Published Online: May 30, 2016
  • Article Type: Research Article
  • Received: May 11, 2014
  • Revised: March 18, 2016
  • Accepted: April 11, 2016
  • DOI: 10.5812/jjm.16765

To Cite: Guojun Y, Yuping H, Yan J, Kaichun L, Haiyang X. A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa, Jundishapur J Microbiol. 2016 ; 9(6):e16765. doi: 10.5812/jjm.16765.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Anastas P, Kirchchoff M, Williamson T. Spinosad-a new natural product for insect control. Green Chemistry. 1999; 1(4)
  • 2. Hahn DR, Gustafson G, Waldron C, Bullard B, Jackson JD, Mitchell J. Butenyl-spinosyns, a natural example of genetic engineering of antibiotic biosynthetic genes. J Ind Microbiol Biotechnol. 2006; 33(2): 94-104[DOI][PubMed]
  • 3. Mayes MA, Thompson GD, Husband B, Miles MM. Spinosad toxicity to pollinators and associated risk. Rev Environ Contam Toxicol. 2003; 179: 37-71[PubMed]
  • 4. Mertz FP, Yao RC. Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J System Bacteriol. 1990; 40(1): 34-9[DOI]
  • 5. Bai Y, Zhou P, Fan P, Zhu Y, Tong Y, Wang H, et al. Four‐stage dissolved oxygen strategy based on multi‐scale analysis for improving spinosad yield by Saccharopolyspora spinosa ATCC49460. Microb Biotechnol. 2015; 8(3): 561-8[DOI]
  • 6. Galm U, Sparks T. Natural product derived insecticides: discovery and development of spinetoram. J Ind Microbiol Biotechnol. 2016; 43(2-3): 185-93[DOI]
  • 7. Jha A, Pokhrel A, Chaudhary A, Park S, Cho W, Sohng J. Metabolic engineering of rational screened Saccharopolyspora spinosa for the enhancement of spinosyns A and D production. Mol Cells. 2014; 37(10): 727-733[DOI]
  • 8. Liang Y, Lu W, Wen J. Improvement of Saccharopolyspora spinosa and the kinetic analysis for spinosad production. Appl Biochem Biotechnol. 2009; 152(3): 440-8[DOI]
  • 9. Luo Y, Kou X, Ding X, Hu S, Tang Y, Li W, et al. Promotion of spinosad biosynthesis by chromosomal integration of the Vitreoscilla hemoglobin gene in Saccharopolyspora spinosa. Sci China Life Sci. 2012; 55(2): 172-80[DOI]
  • 10. Zhang X, Xue C, Zhao F, Li D, Yin J, Zhang C, et al. Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in Saccharopolyspora spinosa. Microbial cell factories. 2014; 13(1): 1[DOI]
  • 11. Strobel Jr RJ, Nakatsukasa WM. Response surface methods for optimizingSaccharopolyspora spinosa, a novel macrolide producer. Industrial Microbiol J. 1993; 11(2): 121-7
  • 12. Zhihua J, CHENG X, Peilin C. Effects of glucose and phosphate on spinosad fermentation by Saccharopolyspora spinosa. Chinese J Chemical Eng. 2006; 14(4): 542-6
  • 13. Li N Z, Guo W, Xiong J, Song W, Song Y. Study on the fermentation media for spinosad production. Nat. Sci. Ed. 2010; 38(1): 216-34
  • 14. Yan L, Wen-yu L, Jian-ping W. Application of Minitab software on the optimization of spinosad fermentation. Chinese J Antibiotics. 2008; 11: 4
  • 15. Dai Peng X. Study on the fermentation of production strains spinosads. J Huazhong Agri Univ. 2006;
  • 16. Madduri K, Waldron C, Merlo DJ. Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa. J Bacteriol. 2001; 183(19): 5632-8[DOI][PubMed]
  • 17. He Y, Hu X, Lin K. Screening and breeding of production strains of spinosad. Chinese J Biol Con. 2005; 22(1): 37-9
  • 18. Wang C, Zhang X, Chen Z, Wen Y, Song Y. Strain construction for enhanced production of spinosad via intergeneric protoplast fusion. Can J Microbiol. 2009; 55(9): 1070-5[DOI][PubMed]
  • 19. Huang Y, Zhao C, Zhang Q,, Song Y, Zhang X. Effects of expression of maltose transport related genes on cell growth and spinosad production in Saccharopolyspora spinosa. J Agricultural Biotechnol. 2015; 23(11): 1438-44
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