Jundishapur Journal of Microbiology

Published by: Kowsar

Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae

Minoo Tavakoli 1 , * , Saeed Bouzari 2 , Seyed Davar Siadat 3 , Shahin Najar Peerayeh 4 and Anis Jafari 2
Authors Information
1 Fars Science and Research Branch, Islamic Azad University, Marvdasht, IR Iran
2 Department of Molecular Biology, Pasteur Institute of Iran, Tehran, IR Iran
3 Department of Lung Diseases, Pasteur Institute of Iran, Tehran, IR Iran
4 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: April 01, 2015, 8 (4); e16377
  • Published Online: April 18, 2015
  • Article Type: Research Article
  • Received: December 8, 2013
  • Revised: May 4, 2014
  • Accepted: May 25, 2014
  • DOI: 10.5812/jjm.8(4)2015.16377

To Cite: Tavakoli M, Bouzari S, Siadat S D, Najar Peerayeh S, Jafari A. Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae, Jundishapur J Microbiol. 2015 ; 8(4):e16377. doi: 10.5812/jjm.8(4)2015.16377.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Silva MEB, Marin JM. Verification of the Presence of Capsule Gene Sequences in Nasopharyngeal Isolates of Nontypeable Haemophilus Influenzae from Healthy Children at a Brazilian Day Care Center. Braz J Microbiol. 2001; 32(3): 233-5
  • 2. Singh B, Al-Jubair T, Morgelin M, Thunnissen MM, Riesbeck K. The unique structure of Haemophilus influenzae protein E reveals multiple binding sites for host factors. Infect Immun. 2013; 81(3): 801-14[DOI][PubMed]
  • 3. Ryan KJ, Ray CG. Sherris Medical Microbiology. 2004;
  • 4. Singh B, Brant M, Kilian M, Hallstrom B, Riesbeck K. Protein E of Haemophilus influenzae is a ubiquitous highly conserved adhesin. J Infect Dis. 2010; 201(3): 414-9[DOI][PubMed]
  • 5. van Ketel RJ, de Wever B, van Alphen L. Detection of Haemophilus influenzae in cerebrospinal fluids by polymerase chain reaction DNA amplification. J Med Microbiol. 1990; 33(4): 271-6[PubMed]
  • 6. Ueyama T, Kurono Y, Shirabe K, Takeshita M, Mogi G. High incidence of Haemophilus influenzae in nasopharyngeal secretions and middle ear effusions as detected by PCR. J Clin Microbiol. 1995; 33(7): 1835-8[PubMed]
  • 7. Hassan-King M, Adegbola R, Baldeh I, Mulholland K, Omosigho C, Oparaugo A, et al. A polymerase chain reaction for the diagnosis of Haemophilus influenzae type b disease in children and its evaluation during a vaccine trial. Pediatr Infect Dis J. 1998; 17(4): 309-12[PubMed]
  • 8. Yadav MC, Chakraborti A, Ray P, Sapru S, Majumdar S, Narang A. Rapid detection of Haemophilus influenzae by hel gene polymerase chain reaction. Lett Appl Microbiol. 2003; 37(3): 190-5[PubMed]
  • 9. Lysenko ES, Ratner AJ, Nelson AL, Weiser JN. The role of innate immune responses in the outcome of interspecies competition for colonization of mucosal surfaces. PLoS Pathog. 2005; 1(1)[DOI][PubMed]
  • 10. Poolman JT, Bakaletz L, Cripps A, Denoel PA, Forsgren A, Kyd J, et al. Developing a nontypeable Haemophilus influenzae (NTHi) vaccine. Vaccine. 2000; 19 Suppl 1-15[PubMed]
  • 11. Ronander E, Brant M, Janson H, Sheldon J, Forsgren A, Riesbeck K. Identification of a novel Haemophilus influenzae protein important for adhesion to epithelial cells. Microbes Infect. 2008; 10(1): 87-96[DOI][PubMed]
  • 12. Leyton DL, Rossiter AE, Henderson IR. From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis. Nat Rev Microbiol. 2012; 10(3): 213-25[DOI][PubMed]
  • 13. Hallstrom T, Blom AM, Zipfel PF, Riesbeck K. Nontypeable Haemophilus influenzae protein E binds vitronectin and is important for serum resistance. J Immunol. 2009; 183(4): 2593-601[DOI][PubMed]
  • 14. Levine OS, Schuchat A, Schwartz B, Wenger JD. Genetic protocol for population-based surveillance of Haemophilus influenzae type B. 1996;
  • 15. Sambrook J, Russell D. Molecular Cloning: a laboratory manual. 2001;
  • 16. Murphy TF, Kirkham C, Lesse AJ. Construction of a mutant and characterization of the role of the vaccine antigen P6 in outer membrane integrity of nontypeable Haemophilus influenzae. Infect Immun. 2006; 74(9): 5169-76[DOI][PubMed]
  • 17. Giufre M, Carattoli A, Cardines R, Mastrantonio P, Cerquetti M. Variation in expression of HMW1 and HMW2 adhesins in invasive nontypeable Haemophilus influenzae isolates. BMC Microbiol. 2008; 8: 83[PubMed]
  • 18. St Geme J3, Falkow S, Barenkamp SJ. High-molecular-weight proteins of nontypable Haemophilus influenzae mediate attachment to human epithelial cells. Proc Natl Acad Sci U S A. 1993; 90(7): 2875-9[PubMed]
  • 19. Reddy MS, Bernstein JM, Murphy TF, Faden HS. Binding between outer membrane proteins of nontypeable Haemophilus influenzae and human nasopharyngeal mucin. Infect Immun. 1996; 64(4): 1477-9[PubMed]
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