Jundishapur Journal of Microbiology

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Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein

Pezhman Mahmoodi 1 , Masoud Reza Seyfi Abad Shapouri 1 , * , Masoud Ghorbanpour 1 , Mohammad Rahim Haji Hajikolaei 2 , Mohsen Lotfi 3 , Mahdi Pourmahdi Boroujeni 4 and Maryam Daghari 1
Authors Information
1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
3 Quality Control Management, Razi Vaccine and Serum Research Institute, Karaj, IR Iran
4 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: March 01, 2015, 8 (3); e14311
  • Published Online: January 14, 2015
  • Article Type: Research Article
  • Received: August 25, 2013
  • Revised: October 24, 2013
  • Accepted: November 27, 2013
  • DOI: 10.5812/jjm.14311

To Cite: Mahmoodi P, Seyfi Abad Shapouri M R, Ghorbanpour M, Haji Hajikolaei M R, Lotfi M, et al. Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein, Jundishapur J Microbiol. 2015 ; 8(3):e14311. doi: 10.5812/jjm.14311.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnote
References
  • 1. Greiser-Wilke I, Grummer B, Moennig V. Bovine viral diarrhoea eradication and control programmes in Europe. Biologicals. 2003; 31(2): 113-8[DOI]
  • 2. Presi P, Heim D. BVD eradication in Switzerland--a new approach. Vet Microbiol. 2010; 142(1-2): 137-42[DOI][PubMed]
  • 3. Edwards S. The diagnosis of bovine virus diarrhoea-mucosal disease in cattle. Rev Sci Tech. 1990; 9(1): 115-30[PubMed]
  • 4. Sandvik T. Laboratory diagnostic investigations for bovine viral diarrhoea virus infections in cattle. Vet Microbiol. 1999; 64(2-3): 123-34[PubMed]
  • 5. Collett MS. Molecular genetics of pestiviruses. Comp Immunol Microbiol Infect Dis. 1992; 15(3): 145-54[PubMed]
  • 6. Collett MS, Wiskerchen M, Welniak E, Belzer SK. Bovine viral diarrhea virus genomic organization. Arch Virol Suppl. 1991; 3: 19-27[PubMed]
  • 7. Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 1989; 17(12): 4713-30[PubMed]
  • 8. Wiskerchen M, Collett MS. Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing. Virology. 1991; 184(1): 341-50[PubMed]
  • 9. Agapov EV, Murray CL, Frolov I, Qu L, Myers TM, Rice CM. Uncleaved NS2-3 is required for production of infectious bovine viral diarrhea virus. J Virol. 2004; 78(5): 2414-25[PubMed]
  • 10. Donis RO, Corapi WV, Dubovi EJ. Bovine viral diarrhea virus proteins and their antigenic analyses. Arch Virol Suppl. 1991; 3: 29-40[PubMed]
  • 11. Bolin SR, Ridpath JF. Specificity of neutralizing and precipitating antibodies induced in healthy calves by monovalent modified-live bovine viral diarrhea virus vaccines. Am J Vet Res. 1989; 50(6): 817-21[PubMed]
  • 12. Mahmoodi P, Shapouri MR, Ghorbanpour M, Ekhtelat M, Hajikolaei MR, Lotfi M, et al. Epitope mapping of bovine viral diarrhea virus nonstructural protein 3. Vet Immunol Immunopathol. 2014; 161(3-4): 232-9[DOI][PubMed]
  • 13. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). 2008;
  • 14. Howard CJ, Clarke MC, Brownlie J. An Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of antibodies to Bovine Viral Diarrhoea Virus (BVDV) in cattle sera. Veterin Microbiol. 1985; 10(4): 359-69[DOI]
  • 15. Justewicz DM, Magar R, Marsolais G, Lecomte J. Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera. Vet Immunol Immunopathol. 1987; 14(4): 377-84[PubMed]
  • 16. Lecomte C, Pin JJ, De Moerlooze L, Vandenbergh D, Lambert AF, Pastoret PP, et al. ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies. Vet Microbiol. 1990; 23(1-4): 193-201[PubMed]
  • 17. Reddy JR, Kwang J, Okwumabua O, Kapil S, Loughin TM, Lechtenberg KF, et al. Application of recombinant bovine viral diarrhea virus proteins in the diagnosis of bovine viral diarrhea infection in cattle. Vet Microbiol. 1997; 57(2-3): 119-33[PubMed]
  • 18. Vanderheijden N, De Moerlooze L, Vandenbergh D, Chappuis G, Renard A, Lecomte C. Expression of the bovine viral diarrhoea virus Osloss p80 protein: its use as ELISA antigen for cattle serum antibody detection. J Gen Virol. 1993; 74 ( Pt 7): 1427-31[PubMed]
  • 19. Chimeno Zoth S, Taboga O. Multiple recombinant ELISA for the detection of bovine viral diarrhoea virus antibodies in cattle sera. J Virol Methods. 2006; 138(1-2): 99-108[DOI][PubMed]
  • 20. Beaudeau F, Belloc C, Seegers H, Assie S, Sellal E, Joly A. Evaluation of a blocking ELISA for the detection of bovine viral diarrhoea virus (BVDV) antibodies in serum and milk. Vet Microbiol. 2001; 80(4): 329-37[PubMed]
  • 21. Bhatia S, Sood R, Mishra N, Pattnaik B, Pradhan HK. Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes. Res Vet Sci. 2008; 85(1): 39-45[DOI][PubMed]
  • 22. Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999; 8(8): 1668-74[DOI][PubMed]
  • 23. Sachdev D, Chirgwin JM. Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin. Protein Expr Purif. 1998; 12(1): 122-32[DOI][PubMed]
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