Jundishapur Journal of Microbiology

Published by: Kowsar

Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes

Shabnam Sadoogh Abbasian 1 , Ehsanollah Ghaznavi Rad 2 , Neda Akbari 3 , Mohammad Reza Zolfaghari 4 , Iraj pakzad 5 and Hamid Abtahi 1 , *
Authors Information
1 Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, IR Iran
2 Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran
3 Department of Microbiology, Faculty of Science, Arak branch, Islamic Azad University, Arak, IR Iran
4 Department of Microbiology, Qom Branch, Islamic Azad University, Qom, IR Iran
5 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 01, 2015, 8 (1); e13653
  • Published Online: December 9, 2014
  • Article Type: Research Article
  • Received: July 17, 2013
  • Revised: June 3, 2014
  • Accepted: June 5, 2014
  • DOI: 10.5812/jjm.13653

To Cite: Sadoogh Abbasian S, Ghaznavi Rad E, Akbari N, Zolfaghari M R, pakzad I, et al. Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes, Jundishapur J Microbiol. 2015 ; 8(1):e13653. doi: 10.5812/jjm.13653.

Abstract
Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Bisno AL, Brito MO, Collins CM. Molecular basis of group A streptococcal virulence. Lancet Infect Dis. 2003; 3(4): 191-200[PubMed]
  • 2. Douglas B, Muchmore MD, Daniel E. Review of the Mechanism of Action and Clinical Efficacy of Recombinant Human Hyaluronidase Coadministration with Current Prandial Insulin Formulations. J Diabetes Sci Tech. 2010; : 419-28
  • 3. Girish KS, Kemparaju K. The magic glue hyaluronan and its eraser hyaluronidase: a biological overview. Life Sci. 2007; 80(21): 1921-43[DOI][PubMed]
  • 4. Stern R, Jedrzejas MJ. Hyaluronidases: their genomics, structures, and mechanisms of action. Chem Rev. 2006; 106(3): 818-39[DOI][PubMed]
  • 5. Hynes WL, Dixon AR, Walton SL, Aridgides LJ. The extracellular hyaluronidase gene (hylA) of Streptococcus pyogenes. FEMS Microbiol Lett. 2000; 184(1): 109-12[PubMed]
  • 6. Park CY, Kim EH, Choi SY, Tran TD, Kim IH, Kim SN, et al. Virulence attenuation of Streptococcus pneumoniae clpP mutant by sensitivity to oxidative stress in macrophages via an NO-mediated pathway. J Microbiol. 2010; 48(2): 229-35[DOI][PubMed]
  • 7. Wu LX, Huang WX, Luo T, Sun XP. [Construction of hyaluronidase gene mutant of Enterococcus faecium and the function of hyaluronidase gene]. Zhonghua Yi Xue Za Zhi. 2007; 87(40): 2852-5[PubMed]
  • 8. Boraston AB, Ficko-Blean E, Healey M. Carbohydrate recognition by a large sialidase toxin from Clostridium perfringens. Biochemistry. 2007; 46(40): 11352-60[DOI][PubMed]
  • 9. Oettl M, Hoechstetter J, Asen I, Bernhardt G, Buschauer A. Comparative characterization of bovine testicular hyaluronidase and a hyaluronate lyase from Streptococcus agalactiae in pharmaceutical preparations. Eur J Pharm Sci. 2003; 18(3-4): 267-77[PubMed]
  • 10. Pierre G, Salah R, Gardarin C, Traikia M, Petit E, Delort AM, et al. Enzymatic degradation and bioactivity evaluation of C-6 oxidized chitosan. Int J Biol Macromol. 2013; 60: 383-92[DOI][PubMed]
  • 11. Abdelhak D. Alternative method for genetic transformation of Pasteurella multocida X73 using a hyaluronidase-producing Staphylococcus aureus strain. J Microbiol Methods. 2009; 78(1): 25-7[DOI][PubMed]
  • 12. Girish KS, Shashidharamurthy R, Nagaraju S, Gowda TV, Kemparaju K. Isolation and characterization of hyaluronidase a "spreading factor" from Indian cobra (Naja naja) venom. Biochimie. 2004; 86(3): 193-202[DOI][PubMed]
  • 13. Markovic-Housley Z, Miglierini G, Soldatova L, Rizkallah PJ, Muller U, Schirmer T. Crystal structure of hyaluronidase, a major allergen of bee venom. Structure. 2000; 8(10): 1025-35[PubMed]
  • 14. Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res. 2003; 23(1A): 363-98[PubMed]
  • 15. Hamada S, Devys JM, Xuan TH, Ganem S, Sahel JA, Heran F, et al. Role of hyaluronidase in diplopia after peribulbar anesthesia for cataract surgery. Ophthalmology. 2005; 112(5): 879-82[DOI][PubMed]
  • 16. Stern R. Hyaluronidases in cancer biology. Semin Cancer Biol. 2008; 18(4): 275-80[DOI][PubMed]
  • 17. Lokeshwar VB, Cerwinka WH, Isoyama T, Lokeshwar BL. HYAL1 hyaluronidase in prostate cancer: a tumor promoter and suppressor. Cancer Res. 2005; 65(17): 7782-9[DOI][PubMed]
  • 18. Kimura M, Kim E, Kang W, Yamashita M, Saigo M, Yamazaki T, et al. Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization. Biol Reprod. 2009; 81(5): 939-47[DOI][PubMed]
  • 19. Palte HD, Greenbaum S, Gayer S. Recombinant hyaluronidase. Clin Experiment Ophthalmol. 2014; 42(3): 298[DOI][PubMed]
  • 20. Girish KS, Jagadeesha DK, Rajeev KB, Kemparaju K. Snake venom hyaluronidase: an evidence for isoforms and extracellular matrix degradation. Mol Cell Biochem. 2002; 240(1-2): 105-10[PubMed]
  • 21. Stern M, Stern R. An ELISA-like assay for hyaluronidase and hyaluronidase inhibitors. Matrix. 1992; 12(5): 397-403[PubMed]
  • 22. Takahashi T, Ikegami-Kawai M, Okuda R, Suzuki K. A fluorimetric Morgan-Elson assay method for hyaluronidase activity. Anal Biochem. 2003; 322(2): 257-63
  • 23. Chen SS, Hsu DS, Hoffman P. A new micro-assay for hyaluronidase activity. Clin Chim Acta. 1979; 95(2): 277-84[PubMed]
  • 24. Rigouin C, Ladrat CD, Sinquin C, Colliec-Jouault S, Dion M. Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides. Carbohyd Polym 2009; 76: 279-284
  • 25. Jaques R. A Biological Method of Assay of hyaluronidase. 1952;
  • 26. Michael S, Robert S. An ELISA-Like Assay for Hyaluronidase and Hyaluronidase Inhibitors. Gustav Fischer Verlag. 1992; : 397-403
  • 27. Stair Nawy S, Csóka AB, Mio K, Stern R. Hyaluronidase Activity and Hyaluronidase Inhibitors: Assay Using a Microtiter-Based System. Meth Molecul Biol. 2001; : 383-9
  • 28. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A Laboratory Manual. 2001;
  • 29. Sadoogh Abbasian S, Abtahi H, Zolfaghari MR, Soufian S, Ghaznavi- Rad E. Cloning, expression, purification and antigenic evaluation of hyaluronidase antigenic fragments recombinant protein of streptococcus pyogenes. African J Biotech. 2012; 11(9): 2376-80
  • 30. Dorfman A. Methods in Enzymology. Mucopolysaccharidases. 1955; 1: 166-73[DOI]
  • 31. Kigawa T, Yabuki T, Matsuda N, Matsuda T, Nakajima R, Tanaka A, et al. Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. J Struct Funct Genomics. 2004; 5(1-2): 63-8[DOI][PubMed]
  • 32. Mello BA, Tu Y. Effects of adaptation in maintaining high sensitivity over a wide range of backgrounds for Escherichia coli chemotaxis. Biophys J. 2007; 92(7): 2329-37[DOI][PubMed]
  • 33. Galloway CA, Sowden MP, Smith HC. Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase. Biotechniques. 2003; 34(3): 524-6-530[PubMed]
  • 34. Chae YK, Cho KS, Chun W, Lee K. Protein production by stationary phase induction (SPI). Protein Pept Lett. 2003; 10(4): 369-74[PubMed]
  • 35. Mahmoudi S, Abtahi H, Bahador A, Mosayebi G, Salmanian A. Teymuri M. Optimizing of nutrients for high level expression of recombinant streptokinase using pET32a expression system. J Clinic Med. 2012; 7(3)
  • 36. Jiang X, Chen H, Yang W, Liu Y, Liu W, Wei J, et al. Functional expression and characterization of an acidic actinoporin from sea anemone Sagartia rosea. Biochem Biophys Res Commun. 2003; 312(3): 562-70[DOI][PubMed]
  • 37. Narang A, Pilyugin SS. Bistability of the lac operon during growth of Escherichia coli on lactose and lactose+glucose. Bull Math Biol. 2008; 70(4): 1032-64[DOI][PubMed]
  • 38. Shrestha P, Holland TM, Bundy BC. Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing. Biotechniques. 2012; 53(3): 163-74[DOI][PubMed]
  • 39. Hynes WL, Walton SL. Hyaluronidases of Gram-positive bacteria. FEMS Microbiol Lett. 2000; 183(2): 201-7[PubMed]
  • 40. Pearce RH. The turbidimetric estimation of hyaluronidase. Biochem J. 1953; 55(3): 467-72[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments