Jundishapur Journal of Microbiology

Published by: Kowsar

Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

Reza Taherkhani 1 , Fatemeh Farshadpour 1 , Manoochehr Makvandi 1 , * and Ali Reza Samarbafzadeh 1
Authors Information
1 Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: September 01, 2014, 7 (11); e12351
  • Published Online: September 1, 2014
  • Article Type: Research Article
  • Received: May 20, 2013
  • Revised: September 22, 2013
  • Accepted: October 5, 2013
  • DOI: 10.5812/jjm.12351

To Cite: Taherkhani R, Farshadpour F, Makvandi M, Samarbafzadeh A R. Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells, Jundishapur J Microbiol. 2014 ; 7(11):e12351. doi: 10.5812/jjm.12351.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Material and Methods
4. Results
5. Discussion
  • 1. Kaisho T, Akira S. Toll-like receptors as adjuvant receptors. Biochim Biophys Acta. 2002; 1589(1): 1-13[PubMed]
  • 2. Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity. Cell. 2006; 124(4): 783-801[DOI][PubMed]
  • 3. Takeda K, Kaisho T, Akira S. Toll-like receptors. Annu Rev Immunol. 2003; 21: 335-76[DOI][PubMed]
  • 4. Pasare C, Medzhitov R. Toll-like receptors: linking innate and adaptive immunity. Microbes Infect. 2004; 6(15): 1382-7[DOI][PubMed]
  • 5. Huleatt JW, Jacobs AR, Tang J, Desai P, Kopp EB, Huang Y, et al. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine. 2007; 25(4): 763-75[DOI][PubMed]
  • 6. Coffman RL, Sher A, Seder RA. Vaccine adjuvants: putting innate immunity to work. Immunity. 2010; 33(4): 492-503[DOI][PubMed]
  • 7. Gewirtz AT, Simon PO, Jr, Schmitt CK, Taylor LJ, Hagedorn CH, O'Brien AD, et al. Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response. J Clin Invest. 2001; 107(1): 99-109[DOI][PubMed]
  • 8. Gewirtz AT. Flag in the crossroads: flagellin modulates innate and adaptive immunity. Curr Opin Gastroenterol. 2006; 22(1): 8-12[PubMed]
  • 9. Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, Goodlett DR, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 2001; 410(6832): 1099-103[DOI][PubMed]
  • 10. Gewirtz AT, Navas TA, Lyons S, Godowski PJ, Madara JL. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol. 2001; 167(4): 1882-5[PubMed]
  • 11. Honko AN, Mizel SB. Effects of flagellin on innate and adaptive immunity. Immunol Res. 2005; 33(1): 83-101[DOI][PubMed]
  • 12. Mizel SB, Bates JT. Flagellin as an adjuvant: cellular mechanisms and potential. J Immunol. 2010; 185(10): 5677-82[DOI][PubMed]
  • 13. Bates JT, Honko AN, Graff AH, Kock ND, Mizel SB. Mucosal adjuvant activity of flagellin in aged mice. Mech Ageing Dev. 2008; 129(5): 271-81[DOI][PubMed]
  • 14. Cuadros C, Lopez-Hernandez FJ, Dominguez AL, McClelland M, Lustgarten J. Flagellin fusion proteins as adjuvants or vaccines induce specific immune responses. Infect Immun. 2004; 72(5): 2810-6[PubMed]
  • 15. Honko AN, Sriranganathan N, Lees CJ, Mizel SB. Flagellin is an effective adjuvant for immunization against lethal respiratory challenge with Yersinia pestis. Infect Immun. 2006; 74(2): 1113-20[DOI][PubMed]
  • 16. Chaung HC, Cheng LT, Hung LH, Tsai PC, Skountzou I, Wang B, et al. Salmonella flagellin enhances mucosal immunity of avian influenza vaccine in chickens. Vet Microbiol. 2012; 157(1-2): 69-77[DOI][PubMed]
  • 17. Huleatt JW, Nakaar V, Desai P, Huang Y, Hewitt D, Jacobs A, et al. Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellin. Vaccine. 2008; 26(2): 201-14[DOI][PubMed]
  • 18. Vijay-Kumar M, Aitken JD, Sanders CJ, Frias A, Sloane VM, Xu J, et al. Flagellin Treatment Protects against Chemicals, Bacteria, Viruses, and Radiation. J Immunol. 2008; 180(12): 8280-5
  • 19. Wang W, Manmohan S. Selection of Adjuvants for Enhanced Vaccine Potency. W J Vac. 2011; 1: 33-78
  • 20. Vogel FR,, Hem SL. Immunological Adjuants 2008;
  • 21. Hedlund J, Langer B, Konradsen HB, Ortqvist A. Negligible adjuvant effect for antibody responses and frequent adverse events associated with IL-12 treatment in humans vaccinated with pneumococcal polysaccharide. Vaccine. 2001; 20(1-2): 164-9[PubMed]
  • 22. Reichhart JM. TLR5 takes aim at bacterial propeller. Nat Immunol. 2003; 4(12): 1159-60[DOI][PubMed]
  • 23. Delaney KN, Phipps JP, Johnson JB, Mizel SB. A recombinant flagellin-poxvirus fusion protein vaccine elicits complement-dependent protection against respiratory challenge with vaccinia virus in mice. Viral Immunol. 2010; 23(2): 201-10
  • 24. Aw D, Silva AB, Palmer DB. Immunosenescence: emerging challenges for an ageing population. Immunology. 2007; 120(4): 435-46[DOI][PubMed]
  • 25. Krieg AM. CpG motifs in bacterial DNA and their immune effects. Annu Rev Immunol. 2002; 20: 709-60
  • 26. Applequist SE, Rollman E, Wareing MD, Liden M, Rozell B, Hinkula J, et al. Activation of innate immunity, inflammation, and potentiation of DNA vaccination through mammalian expression of the TLR5 agonist flagellin. J Immunol. 2005; 175(6): 3882-91[PubMed]
  • 27. Sallberg M, Townsend K, Chen M, O'Dea J, Banks T, Jolly DJ, et al. Characterization of humoral and CD4+ cellular responses after genetic immunization with retroviral vectors expressing different forms of the hepatitis B virus core and e antigens. J Virol. 1997; 71(7): 5295-303[PubMed]
  • 28. McDonald WF, Huleatt JW, Foellmer HG, Hewitt D, Tang J, Desai P, et al. A West Nile virus recombinant protein vaccine that coactivates innate and adaptive immunity. J Infect Dis. 2007; 195(11): 1607-17[DOI][PubMed]
  • 29. Bargieri DY, Rosa DS, Braga CJ, Carvalho BO, Costa FT, Espindola NM, et al. New malaria vaccine candidates based on the Plasmodium vivax Merozoite Surface Protein-1 and the TLR-5 agonist Salmonella Typhimurium FliC flagellin. Vaccine. 2008; 26(48): 6132-42[DOI][PubMed]
  • 30. Ben-Yedidia T, Marcus H, Reisner Y, Arnon R. Intranasal administration of peptide vaccine protects human/mouse radiation chimera from influenza infection. Int Immunol. 1999; 11(7): 1043-51[PubMed]
  • 31. Lee SE, Kim SY, Jeong BC, Kim YR, Bae SJ, Ahn OS, et al. A bacterial flagellin, Vibrio vulnificus FlaB, has a strong mucosal adjuvant activity to induce protective immunity. Infect Immun. 2006; 74(1): 694-702[DOI][PubMed]
  • 32. Ben-Yedidia T, Arnon R. Epitope-based vaccine against influenza. Expert Rev Vaccines. 2007; 6(6): 939-48[DOI][PubMed]
  • 33. Adar Y, Singer Y, Levi R, Tzehoval E, Perk S, Banet-Noach C, et al. A universal epitope-based influenza vaccine and its efficacy against H5N1. Vaccine. 2009; 27(15): 2099-107[DOI][PubMed]
  • 34. Turley CB, Rupp RE, Johnson C, Taylor DN, Wolfson J, Tussey L, et al. Safety and immunogenicity of a recombinant M2e-flagellin influenza vaccine (STF2.4xM2e) in healthy adults. Vaccine. 2011; 29(32): 5145-52[DOI][PubMed]
  • 35. Pino O, Martin M, Michalek SM. Cellular mechanisms of the adjuvant activity of the flagellin component FljB of Salmonella enterica Serovar Typhimurium to potentiate mucosal and systemic responses. Infect Immun. 2005; 73(10): 6763-70[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments