Jundishapur Journal of Microbiology

Published by: Kowsar

Antimicrobial Susceptibilities and Distribution of Resistance Genes for β-Lactams in Streptococcus pneumoniae Isolated in Hamadan

Mohammad Najafi Mosleh 1 , Marzieh Gharibi 1 , * , Mohammad Yousef Alikhani 1 , Massoud Saidijam 2 and Giti Kalantarian 3
Authors Information
1 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan,IR Iran
3 Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: October 01, 2014, 7 (10); e12714
  • Published Online: October 1, 2014
  • Article Type: Research Article
  • Received: September 8, 2013
  • Revised: March 5, 2014
  • Accepted: April 15, 2014
  • DOI: 10.5812/jjm.12714

To Cite: Najafi Mosleh M, Gharibi M, Alikhani M Y, Saidijam M, Kalantarian G. Antimicrobial Susceptibilities and Distribution of Resistance Genes for β-Lactams in Streptococcus pneumoniae Isolated in Hamadan, Jundishapur J Microbiol. 2014 ; 7(10):e12714. doi: 10.5812/jjm.12714.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. van der Poll T, Opal SM. Pathogenesis, treatment, and prevention of pneumococcal pneumonia. Lancet. 2009; 374(9700): 1543-56[DOI][PubMed]
  • 2. Chiavolini D, Pozzi G, Ricci S. Animal models of Streptococcus pneumoniae disease. Clin Microbiol Rev. 2008; 21(4): 666-85[DOI][PubMed]
  • 3. Moreillon P, Wenger A, Caldelari I. [Pneumococcal antibiotic resistance]. Rev Med Suisse Romande. 2000; 120(8): 651-9[PubMed]
  • 4. Friedland IR, McCracken GH, Jr. Management of infections caused by antibiotic-resistant Streptococcus pneumoniae. N Engl J Med. 1994; 331(6): 377-82[DOI][PubMed]
  • 5. Appelbaum PC. Resistance among Streptococcus pneumoniae: Implications for drug selection. Clin Infect Dis. 2002; 34(12): 1613-20[DOI][PubMed]
  • 6. Perez-Llarena FJ, Bou G. Beta-lactamase inhibitors: the story so far. Curr Med Chem. 2009; 16(28): 3740-65[PubMed]
  • 7. Consumption of antimicrobial agents and occurrence of antimicrobial resistance in norway 2002;
  • 8. Song JH, Chang HH, Suh JY, Ko KS, Jung SI, Oh WS, et al. Macrolide resistance and genotypic characterization of Streptococcus pneumoniae in Asian countries: a study of the Asian Network for Surveillance of Resistant Pathogens (ANSORP). J Antimicrob Chemother. 2004; 53(3): 457-63[DOI][PubMed]
  • 9. Woodford N, Livermore DM. Infections caused by Gram-positive bacteria: a review of the global challenge. J Infect. 2009; 59 Suppl 1-16[DOI][PubMed]
  • 10. Livermore DM. Mechanisms of resistance to cephalosporin antibiotics. Drugs. 1987; 34 Suppl 2: 64-88[PubMed]
  • 11. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005; 18(4): 657-86[DOI][PubMed]
  • 12. Piddock LJ. Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev. 2006; 19(2): 382-402[DOI][PubMed]
  • 13. Macheboeuf P, Contreras-Martel C, Job V, Dideberg O, Dessen A. Penicillin binding proteins: key players in bacterial cell cycle and drug resistance processes. FEMS Microbiol Rev. 2006; 30(5): 673-91[DOI][PubMed]
  • 14. Hakenbeck R. Transformation in Streptococcus pneumoniae: mosaic genes and the regulation of competence. Res Microbiol. 2000; 151(6): 453-6[PubMed]
  • 15. Hakenbeck R. Mosaic genes and their role in penicillin-resistant Streptococcus pneumoniae. Electrophoresis. 1998; 19(4): 597-601[DOI][PubMed]
  • 16. Baquero F, Blazquez J, Loza E, Canton R. [Molecular basis of resistance to beta-lactams in infections by Streptococcus pneumoniae]. Med Clin (Barc). 1998; 110 Suppl 1: 8-11[PubMed]
  • 17. Coffey TJ, Dowson CG, Daniels M, Spratt BG. Genetics and molecular biology of beta-lactam-resistant pneumococci. Microb Drug Resist. 1995; 1(1): 29-34[PubMed]
  • 18. Dowson CG, Coffey TJ, Spratt BG. Origin and molecular epidemiology of penicillin-binding-protein-mediated resistance to beta-lactam antibiotics. Trends Microbiol. 1994; 2(10): 361-6[PubMed]
  • 19. Dowson CG, Hutchison A, Woodford N, Johnson AP, George RC, Spratt BG. Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1990; 87(15): 5858-62[PubMed]
  • 20. Karlowsky JA, Thornsberry C, Jones ME, Evangelista AT, Critchley IA, Sahm DF, et al. Factors associated with relative rates of antimicrobial resistance among Streptococcus pneumoniae in the United States: results from the TRUST Surveillance Program (1998-2002). Clin Infect Dis. 2003; 36(8): 963-70[DOI][PubMed]
  • 21. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world. 2003;
  • 22. Ubukata K, Muraki T, Igarashi A, Asahi Y, Konno M. Identification of penicillin and other beta-lactam resistance inStreptococcus pneumoniae by polymerase chain reaction. J Infect Chemother. 1997; 3(4): 190-7[DOI]
  • 23. Ubukata K, Asahi Y, Yamane A, Konno M. Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR. J Clin Microbiol. 1996; 34(3): 592-6[PubMed]
  • 24. Garcia P, Garcia JL, Garcia E, Lopez R. Nucleotide sequence and expression of the pneumococcal autolysin gene from its own promoter in Escherichia coli. Gene. 1986; 43(3): 265-72[PubMed]
  • 25. Jorgensen JH, Howell AW, Maher LA. Quantitative antimicrobial susceptibility testing of Haemophilus influenzae and Streptococcus pneumoniae by using the E-test. J Clin Microbiol. 1991; 29(1): 109-14[PubMed]
  • 26. Baker CN, Stocker SA, Culver DH, Thornsberry C. Comparison of the E Test to agar dilution, broth microdilution, and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. J Clin Microbiol. 1991; 29(3): 533-8[PubMed]
  • 27. Jorgensen JH, Maher LA, Howell AW. Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae. J Clin Microbiol. 1990; 28(3): 430-4[PubMed]
  • 28. Performance Standards for Antimicrobial Disk Susceptibility Test: Approved Standards. 2001;
  • 29. Ubukata K, Iwata S, Sunakawa K. In vitro activities of new ketolide, telithromycin, and eight other macrolide antibiotics against Streptococcus pneumoniae having mefA and ermB genes that mediate macrolide resistance. J Infect Chemother. 2003; 9(3): 221-6[DOI][PubMed]
  • 30. Barroso DE, Godoy D, Castineiras TM, Tulenko MM, Rebelo MC, Harrison LH. beta-Lactam resistance, serotype distribution, and genotypes of meningitis-causing Streptococcus pneumoniae, Rio de Janeiro, Brazil. Pediatr Infect Dis J. 2012; 31(1): 30-6[DOI][PubMed]
  • 31. Moyo SJ, Steinbakk M, Aboud S, Mkopi N, Kasubi M, Blomberg B, et al. Penicillin resistance and serotype distribution of Streptococcus pneumoniae in nasopharyngeal carrier children under 5 years of age in Dar es Salaam, Tanzania. J Med Microbiol. 2012; 61: 952-9[DOI][PubMed]
  • 32. Mokaddas EM, Rotimi VO, Albert MJ. Implications of Streptococcus pneumoniae penicillin resistance and serotype distribution in Kuwait for disease treatment and prevention. Clin Vaccine Immunol. 2008; 15(2): 203-7[DOI][PubMed]
  • 33. Bokaeian M, Khazaei HA, Javadimehr M. Nasopharyngeal Carriage, Antibiotic Resistance and Serotype Distribution of Streptococcus Pneumoniae among Healthy Adolescents in Zahedan. Iran Red Crescent Med J. 2011; 13(5): 328-33[PubMed]
  • 34. Amini Bavil Olyaee S, Sabahi F, Karimi M. PCR optimization: Improving of human cytomegalovirus (HCMV) PCR to achieve a highly sensitive detection method. Iran J Biotech. 2003; 1(1): 59-64
  • 35. Dehnavi E, Ahani Azari M, Hasani S, Nassiry MR, Mohajer M, Khan Ahmadi AR. Genetic variability of calpastatin and calpain genes in Iranian Zel sheep using PCR-RFLP and PCR-SSCP methods. Iran J Biotech. 2012; 10(2): 136-9
  • 36. Fahami S, Kordbacheh P, Moazeni M, Mahmoodi M, Mirhendi H. Species Identification and Strain Typing of Candida Isolates by PCR-RFLP and RAPD-PCR Analysis for Determining the Probable Sources of Nosocomial Infections. Iran Red Crescent Med J. 2014; 12(5): 539-47
  • 37. Soleimani M, Zolfaghari MR, Morovvati A. Development and Comparison of Conventional PCR and SYBR Green Real Time PCR for Detection of Aggregatibacter actinomycetemcomitans and Tannerella forsythensis. Jundishapur J Microbiol. 2013; 6(8)[DOI]
  • 38. Ubukata K, Chiba N, Hasegawa K, Kobayashi R, Iwata S, Sunakawa K. Antibiotic susceptibility in relation to penicillin-binding protein genes and serotype distribution of Streptococcus pneumoniae strains responsible for meningitis in Japan, 1999 to 2002. Antimicrob Agents Chemother. 2004; 48(5): 1488-94[PubMed]
  • 39. Whitney CG, Farley MM, Hadler J, Harrison LH, Lexau C, Reingold A, et al. Increasing prevalence of multidrug-resistant Streptococcus pneumoniae in the United States. N Engl J Med. 2000; 343(26): 1917-24[DOI][PubMed]
  • 40. Chen DK, McGeer A, de Azavedo JC, Low DE. Decreased susceptibility of Streptococcus pneumoniae to fluoroquinolones in Canada. Canadian Bacterial Surveillance Network. N Engl J Med. 1999; 341(4): 233-9[DOI][PubMed]
  • 41. Davidson R, Cavalcanti R, Brunton JL, Bast DJ, de Azavedo JCS, Kibsey P, et al. Resistance to levofloxacin and failure of treatment of pneumococcal pneumonia. N Engl J Med. 2002; 346(10): 747-50
  • 42. Henderson FW, Gilligan PH, Wait K, Goff DA. Nasopharyngeal carriage of antibiotic-resistant pneumococci by children in group day care. J Infect Dis. 1988; 157(2): 256-63[PubMed]
  • 43. Jones N, Huebner R, Khoosal M, Crewe-Brown H, Klugman K. The impact of HIV on Streptococcus pneumoniae bacteraemia in a South African population. AIDS. 1998; 12(16): 2177-84[PubMed]
  • 44. Ng EW, Costa JR, Samiy N, Ruoff KL, Connolly E, Cousins FV, et al. Contribution of pneumolysin and autolysin to the pathogenesis of experimental pneumococcal endophthalmitis. Retina. 2002; 22(5): 622-32[PubMed]
  • 45. Paton JC, Berry AM, Lock RA. Molecular analysis of putative pneumococcal virulence proteins. Microb Drug Resist. 1997; 3(1): 1-10[PubMed]
  • 46. Nava JM, Bella F, Garau J, Lite J, Morera MA, Marti C, et al. Predictive factors for invasive disease due to penicillin-resistant Streptococcus pneumoniae: a population-based study. Clin Infect Dis. 1994; 19(5): 884-90[PubMed]
  • 47. Quach C, Weiss K, Moore D, Rubin E, McGeer A, Low DE. Clinical aspects and cost of invasive Streptococcus pneumoniae infections in children: resistant vs. susceptible strains. Int J Antimicrob Agents. 2002; 20(2): 113-8[PubMed]
  • 48. Schrag SJ, Beall B, Dowell SF. Limiting the spread of resistant pneumococci: biological and epidemiologic evidence for the effectiveness of alternative interventions. Clin Microbiol Rev. 2000; 13(4): 588-601[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments