Jundishapur Journal of Microbiology

Published by: Kowsar

A preliminary Study: Expression of Rhoptry Protein 1 (ROP1) Toxoplasma gondii in Prokaryote System

Zahra Eslamirad 1 , 2 , * , Fatemeh Ghaffarifar 3 , Mana Shojapour 4 , Behzad Khansarinejad 5 and Javid Sadraei 3
Authors Information
1 Department of Parasitology, Arak University of Medical Sciences, Arak, IR Iran
2 Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, IR Iran
3 Depepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
4 Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, IR Iran
5 Department of Microbiology, Arak University of Medical Sciences, Arak, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 01, 2013, 6 (6); e10089
  • Published Online: August 1, 2013
  • Article Type: Research Article
  • Received: January 2, 2013
  • Revised: February 23, 2013
  • Accepted: March 25, 2013
  • DOI: 10.5812/jjm.10089

To Cite: Eslamirad Z, Ghaffarifar F, Shojapour M, Khansarinejad B, Sadraei J. A preliminary Study: Expression of Rhoptry Protein 1 (ROP1) Toxoplasma gondii in Prokaryote System, Jundishapur J Microbiol. 2013 ; 6(6):e10089. doi: 10.5812/jjm.10089.

Abstract
Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Remington JS, McLeod R, Thulliez P, Desmonts G. Infectious Diseases of the Fetus and Newborn Infant. 2006; : 947-1091
  • 2. Jongert E, Roberts CW, Gargano N, Forster-Waldl E, Petersen E. Vaccines against Toxoplasma gondii: challenges and opportunities. Mem Inst Oswaldo Cruz. 2009; 104(2): 252-66[PubMed]
  • 3. Li S, Galvan G, Araujo FG, Suzuki Y, Remington JS, Parmley S. Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens. Clin Diagn Lab Immunol. 2000; 7(5): 781-7[PubMed]
  • 4. Dlugonska H. Toxoplasma rhoptries: unique secretory organelles and source of promising vaccine proteins for immunoprevention of toxoplasmosis. J Biomed Biotechnol. 2008; 2008: 632424[DOI][PubMed]
  • 5. Meissner M, Klaus K. What new cell biology findings could bring to therapeutics: is it time for a phenome-project in Toxoplasma gondii? Mem Inst Oswaldo Cruz. 2009; 104(2): 185-9[PubMed]
  • 6. Bradley PJ, Sibley LD. Rhoptries: an arsenal of secreted virulence factors. Curr Opin Microbiol. 2007; 10(6): 582-7[DOI][PubMed]
  • 7. Garcia JL, Gennari SM, Navarro IT, Machado RZ, Sinhorini IL. Toxoplasma gondii: isolation of tachyzoites rhoptries and incorporation into Iscom. Exp Parasitol. 2004; 108(1-2): 40-6[DOI][PubMed]
  • 8. Bradley PJ, Hsieh CL, Boothroyd JC. Unprocessed Toxoplasma ROP1 is effectively targeted and secreted into the nascent parasitophorous vacuole. Mol Biochem Parasitol. 2002; 125(1-2): 189-93[PubMed]
  • 9. Bhopale GM. Development of a vaccine for toxoplasmosis: current status. Microbes Infect. 2003; 5(5): 457-62[PubMed]
  • 10. Crampton A, Vanniasinkam T. Parasite vaccines: the new generation. Infect Genet Evol. 2007; 7(5): 664-73[DOI][PubMed]
  • 11. Chen G, Guo H, Lu F, Zheng H. Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and preliminary observations on DNA immunity. Chin Med J (Engl). 2001; 114(8): 837-40[PubMed]
  • 12. Eslamirad Z, Dalimi A, Ghaffarifar F, Sharifi Z, Hosseini A Zavaran. Induction of protective immunity against toxoplasmosis in mice by immunization with a plasmid encoding Toxoplama gondii ROP1 gene. Afr J Biotechnol. 2012; 11(35): 8735-8741
  • 13. Guo H, Chen G, Zheng H, Zhou Y, Lu F. [Immune responses in mice vaccinated with recombinant plasmid pcDNA3 containing ROP1 gene from Toxoplasma gondii]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999; 17(6): 334-7[PubMed]
  • 14. Holec-Gasior L, Kur J, Hiszczynska-Sawicka E. GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii-specific immunoglobulin G in humans with acute toxoplasmosis. Clin Vaccine Immunol. 2009; 16(4): 510-4[DOI][PubMed]
  • 15. Guo H, Chen G, Lu F, Chen H, Zheng H. Immunity induced by DNA vaccine of plasmid encoding the rhoptry protein 1 gene combined with the genetic adjuvant of pcIFN-gamma against Toxoplasma gondii in mice. Chin Med J (Engl). 2001; 114(3): 317-20[PubMed]
  • 16. Chen H, Chen G, Zheng H, Guo H. Induction of immune responses in mice by vaccination with Liposome-entrapped DNA complexes encoding Toxoplasma gondii SAG1 and ROP1 genes. Chin Med J (Engl). 2003; 116(10): 1561-6[PubMed]
  • 17. Aubert D, Maine GT, Villena I, Hunt JC, Howard L, Sheu M, et al. Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J Clin Microbiol. 2000; 38(3): 1144-50[PubMed]
  • 18. Meek B, Jan Diepersloot R, van Gool T, Speijer D, Peek R. Igm recognition of recombinant Toxoplasma gondii antigens by sera of acutely or latently infected humans. Diagn Microbiol Infect Dis . 2003; 45(1): 45-52
  • 19. Pfrepper KI, Enders G, Gohl M, Krczal D, Hlobil H, Wassenberg D, et al. Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin Diagn Lab Immunol. 2005; 12(8): 977-82[DOI][PubMed]
  • 20. Eslamirad Z, Dalimi A, Ghaffarifar F, Sharifi Z. Cloning rhoptry protein 1 (ROP1) gene of Toxoplasma gondii (RH) in expression vector. Archives of Razi. 2009; 63(2): 11-17
  • 21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-5[PubMed]
  • 22. Hoseinian Khosroshahi K, Ghaffarifar F, D'Souza S, Sharifi Z, Dalimi A. Evaluation of the immune response induced by DNA vaccine cocktail expressing complete SAG1 and ROP2 genes against toxoplasmosis. Vaccine. 2011; 29(4): 778-83[DOI][PubMed]
  • 23. Johnson AM, Roberts H, Tenter AM. Evaluation of a recombinant antigen ELISA for the diagnosis of acute toxoplasmosis and comparison with traditional antigen ELISAs. J Med Microbiol. 1992; 37(6): 404-9[PubMed]
  • 24. Araujo PR, Ferreira AW. High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis. Rev Inst Med Trop Sao Paulo. 2010; 52(2): 63-8[PubMed]
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