Jundishapur Journal of Microbiology

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Identification of Leishmania Isolates From Healing and Nonhealing Cutaneous Leishmaniasis Patients Using Internal Transcribed Spacer Region PCR

Sepideh Tolouei 1 , Seyed Hossein Hejazi 1 , * , Kamran Ghaedi 2 and Seyed Javad Hasheminia 3
Authors Information
1 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
2 Department of Biology, School of Sciences, University of Isfahan, Isfahan, IR Iran
3 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: April 01, 2014, 7 (4); e9529
  • Published Online: April 1, 2014
  • Article Type: Research Article
  • Received: December 7, 2012
  • Revised: January 23, 2013
  • Accepted: February 19, 2013
  • DOI: 10.5812/jjm.9529

To Cite: Tolouei S, Hejazi S H, Ghaedi K, Hasheminia S J. Identification of Leishmania Isolates From Healing and Nonhealing Cutaneous Leishmaniasis Patients Using Internal Transcribed Spacer Region PCR, Jundishapur J Microbiol. 2014 ; 7(4):e9529. doi: 10.5812/jjm.9529.

Abstract
Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, Brooker S. Cutaneous leishmaniasis. Lancet Infect Dis. 2007; 7(9): 581-96[DOI][PubMed]
  • 2. Bailey MS, Lockwood DN. Cutaneous leishmaniasis. Clin Dermatol. 2007; 25(2): 203-11[DOI][PubMed]
  • 3. Herwaldt BL. Leishmaniasis. Lancet. 1999; 354(9185): 1191-9[DOI][PubMed]
  • 4. Desjeux P. Worldwide increasing risk factors for leishmaniasis. Med Microbiol Immunol. 2001; 190(1-2): 77-9
  • 5. Desjeux P, Piot B, O'Neill K, Meert JP. [Co-infections of leishmania/HIV in south Europe]. Med Trop (Mars). 2001; 61(2): 187-93[PubMed]
  • 6. Wolday D, Berhe N, Akuffo H, Desjeux P, Britton S. Emerging Leishmania/HIV co-infection in Africa. Med Microbiol Immunol. 2001; 190(1-2): 65-7[PubMed]
  • 7. Yaghoobi-Ershadi MR, Akhavan AA, Abai MR, Ebrahimi B, Zahraei-Ramazani AR, Vafaei-Nezhad R, et al. Epidemiological study in a new focus of cutaneous leishmaniasis in the Islamic Republic of Iran. East Mediterr Health J. 2004; 10(4-5): 688[PubMed]
  • 8. Nadim A, Faghih M. The epidemiology of cutaneous leishmaniasis in the Isfahan province of Iran. I. The reservoir. II. The human disease. Trans R Soc Trop Med Hyg. 1968; 62(4): 534-42[PubMed]
  • 9. Nadim A, Mesghali A, Amini H. Epidemiology of cutaneous leishmaniasis in the Isfahan province of Iran. 3. The vector. Trans R Soc Trop Med Hyg. 1968; 62(4): 543-9[PubMed]
  • 10. Solbach W, Laskay T. The host response to Leishmania infection. Adv Immunol. 2000; 74: 275-317[PubMed]
  • 11. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol. 2006; 44(4): 1435-9[DOI][PubMed]
  • 12. Reithinger R, Dujardin JC. Molecular diagnosis of leishmaniasis: current status and future applications. J Clin Microbiol. 2007; 45(1): 21-5[DOI][PubMed]
  • 13. Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 2003; 47(1): 349-58[PubMed]
  • 14. van Eys GJ, Schoone GJ, Kroon NC, Ebeling SB. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol. 1992; 51(1): 133-42[PubMed]
  • 15. Al-Jawabreh A, Schoenian G, Hamarsheh O, Presber W. Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Trop. 2006; 99(1): 55-61[DOI][PubMed]
  • 16. Shahbazi F, Shahabi S, Kazemi B, Mohebali M, Abadi AR, Zare Z. Evaluation of PCR assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods. Parasitol Res. 2008; 103(5): 1159-62[DOI][PubMed]
  • 17. Yaghoobi-Ershadi MR, Jafari R, Hanafi-Bojd AA. A new epidemic focus of zoonotic cutaneous leishmaniasis in central Iran. Ann Saudi Med. 2004; 24(2): 98-101[PubMed]
  • 18. Ajdary S, Alimohammadian MH, Eslami MB, Kemp K, Kharazmi A. Comparison of the immune profile of nonhealing cutaneous Leishmaniasis patients with those with active lesions and those who have recovered from infection. Infect Immun. 2000; 68(4): 1760-4[PubMed]
  • 19. Blackwell JM. Genetic susceptibility to leishmanial infections: studies in mice and man. Parasitology. 1996; 112 Suppl-74[PubMed]
  • 20. Croft SL, Sundar S, Fairlamb AH. Drug resistance in leishmaniasis. Clin Microbiol Rev. 2006; 19(1): 111-26[DOI][PubMed]
  • 21. Liese J, Schleicher U, Bogdan C. The innate immune response against Leishmania parasites. Immunobiology. 2008; 213(3-4): 377-87[DOI][PubMed]
  • 22. Mahmoodi M, Rajabalian S, Fekri A, Esfandiarpour I. Evaluation of In Vitro Production of IFN-gamma, IL-10, IL-12 and IL-13 by Blood Cells in Patients with Cutaneous Leishmaniasis Lesions. Iran J Allergy Asthma Immunol. 2005; 4(1): 15-21[PubMed]
  • 23. Tolouei S, Ghaedi K, Khamesipour A, Akbari M, Baghaei M, Hasheminia S, et al. IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis. Iran J Parasitol. 2012; 7(1): 18-25[PubMed]
  • 24. Ghasemian M, Maraghi S, Samarbafzadeh AR, Jelowdar A, Kalantari M. The PCR-based detection and identification of the parasites causing human cutaneous leishmaniasis in the Iranian city of Ahvaz. Ann Trop Med Parasitol. 2011; 105(3): 209-15[DOI][PubMed]
  • 25. Mahboudi F, Abolhassan M, Yaran M, Mobtaker H, Azizi M. Identification and differentiation of Iranian Leishmania species by PCR amplification of kDNA. Scand J Infect Dis. 2001; 33(8): 596-8[PubMed]
  • 26. Rahbarian N, Mesgarian A, Mahmoudi Rad M, Hajaran H, Shahbazi F, Mesgarian Z, et al. Identification of leishmania species isolated from human cutaneous leishmaniasis using PCR method. J Res Health Sci. 2009; 9(2): 48-51[PubMed]
  • 27. Saki J, Meamar A, Oormazdi H, Akhlaghi L, Maraghi S, Mohebali M, et al. Mini-exon genotyping of leishmania species in khuzestan province, southwest iran. Iran J Parasitol. 2010; 5(1): 25-34[PubMed]
  • 28. Hejazi H, Nasrifar P, Jamali S, Jahangirnezhad A, Khamesipour A. Identification of Leishmania species using monoclonal antibodies in Isfahan. Iran J Dermatol. 2000; 1(4): 7-11
  • 29. Mohammadi F, Narimani M, Nekoian S, Bidabadi LS, Mohammadi F, Hosseini SM, et al. Identification and Isolation of the Cause of Cutaneous Leishmaniasis in Isfahan Using ITS-PCR Method. J Isfahan Med School. 2012; 30(175)
  • 30. Doudi M, Hejazi SH, Razavi MR, Narimani M, Khanjani S, Eslami G. Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam, Iran. Med Sci Monit. 2010; 16(11)-5[PubMed]
  • 31. Dabirzadeh M, Mirmohammad Sadeghi H, Baghaie M, Hejazi H. Genetic polymorphism of Leishmania major in two hyper endemic regions of Iran revealed by PPIP-PCR and ITS- RFLP. Arch Iran Med. 2012; 15(3): 151-6[PubMed]
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