Jundishapur Journal of Microbiology

Published by: Kowsar

Electrophoretic Patterns of Toxoplasmagondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response

Ahmad Daryani 1 , Mehdi Sharif 1 , Hamed Kalani 1 , * , Alireza Rafiei 2 , Farzad Kalani 2 and Ehsan Ahmadpour 1
Authors Information
1 Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, IR Iran
2 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IR Iran
Article information
  • Jundishapur Journal of Microbiology: April 01, 2014, 7 (4); e9225
  • Published Online: April 1, 2014
  • Article Type: Research Article
  • Received: December 7, 2012
  • Revised: January 23, 2013
  • Accepted: February 17, 2013
  • DOI: 10.5812/jjm.9525

To Cite: Daryani A, Sharif M, Kalani H, Rafiei A, Kalani F, et al. Electrophoretic Patterns of Toxoplasmagondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response, Jundishapur J Microbiol. 2014 ; 7(4):e9225. doi: 10.5812/jjm.9525.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Kim K, Weiss LM. Toxoplasma gondii: the model apicomplexan. Int J Parasitol. 2004; 34(3): 423-32[DOI][PubMed]
  • 2. Hill D, Dubey JP. Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect. 2002; 8(10): 634-40[PubMed]
  • 3. Webster JP, McConkey GA. Toxoplasma gondii-altered host behaviour: clues as to mechanism of action. Folia Parasitol (Praha). 2010; 57(2): 95-104[PubMed]
  • 4. Dubey JP. Toxoplasmosis of animals and humans. 2010; [DOI]
  • 5. Buxton D, Innes EA. A commercial vaccine for ovine toxoplasmosis. Parasitology. 1995; 110 Suppl-6[PubMed]
  • 6. Valcoun A. Continoual cultivation of Toxoplasma gondii on HeLa cells. Folia Parasitol. 1983; 30: 289-94
  • 7. Pettersen EK. Resistance to avirulent Toxoplasma gondii in normal and vaccinated rats. APMIS. 1988; 96(9): 820-4[PubMed]
  • 8. Falcon J, Freyre A. Toxoplasma gondii: prototype immunization of lambs against formation of muscle and brain cysts. Vet Parasitol. 2009; 166(1-2): 15-20[DOI][PubMed]
  • 9. Krahenbuhl JL, Ruskin J, Remington JS. The use of killed vaccines in immunization against an intracellular parasite: Toxoplasma gondii. J Immunol. 1972; 108(2): 425-31[PubMed]
  • 10. Waldeland H, Frenkel JK. Live and killed vaccines against toxoplasmosis in mice. J Parasitol. 1983; 69(1): 60-5[PubMed]
  • 11. Dubey JP, Lunney JK, Shen SK, Kwok OC. Immunity to toxoplasmosis in pigs fed irradiated Toxoplasma gondii oocysts. J Parasitol. 1998; 84(4): 749-52[PubMed]
  • 12. Lin A, Lu S, Chen C, Li S, Chen R, Lu X, et al. [The protective effect against Toxoplasma infection in mice immunized with laser-irradiated Toxoplasma tachyzoites]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999; 17(6): 387-9[PubMed]
  • 13. Duquesne V, Auriault C, Darcy F, Decavel JP, Capron A. Protection of nude rats against Toxoplasma infection by excreted-secreted antigen-specific helper T cells. Infect Immun. 1990; 58(7): 2120-6[PubMed]
  • 14. Daryani A, Hosseini AZ, Dalimi A. Immune responses against excreted/secreted antigens of Toxoplasma gondii tachyzoites in the murine model. Vet Parasitol. 2003; 113(2): 123-34[PubMed]
  • 15. Araujo PR, Ferreira AW. Avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii: immunological marker for acute recent toxoplasmosis. Rev Soc Bras Med Trop. 2008; 41(2): 142-7[PubMed]
  • 16. Costa-Silva TA, Meira CS, Ferreira IM, Hiramoto RM, Pereira-Chioccola VL. Evaluation of immunization with tachyzoite excreted-secreted proteins in a novel susceptible mouse model (A/Sn) for Toxoplasma gondii. Exp Parasitol. 2008; 120(3): 227-34[DOI][PubMed]
  • 17. Saadatnia G, Haj Ghani H, Khoo BY, Maimunah A, Rahmah N. Optimization of Toxoplasma gondii cultivation in VERO cell line. Trop Biomed. 2010; 27(1): 125-30[PubMed]
  • 18. Mémet S, Lilienbaum A, Israël A. Rapid isolation of mouse primary fibroblasts: a tool for the analysis of transgenic mice. Tech Tips Online. 1997; 2(1): 65-6[DOI]
  • 19. Zhang Y, Zhao L, Wang C, Lei B. [Isolation and culture of mouse embryonic fibroblast]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2003; 34(2): 344-6[PubMed]
  • 20. Schade B, Fischer HG. Toxoplasma gondii induction of interleukin-12 is associated with acute virulence in mice and depends on the host genotype. Vet Parasitol. 2001; 100(1-2): 63-74[PubMed]
  • 21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-5[PubMed]
  • 22. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9): 4350-4[PubMed]
  • 23. Daryani A, Hosseini AZ, Sharif M, Dalimi A, Dehghan MH, Ziaei¹ H. Protective role of antigens from peritoneal exudates of infected mice against toxoplasmosis. Iran J Immunol. 2006; 3(2): 78
  • 24. Darcy F, Deslee D, Santoro F, Charif H, Auriault C, Decoster A, et al. Induction of a protective antibody-dependent response against toxoplasmosis by in vitro excreted/secreted antigens from tachyzoites of Toxoplasma gondii. Parasite Immunol. 1988; 10(5): 553-67[PubMed]
  • 25. Park YK, Nam HW. Early recognized antigen (p34) of Toxoplasma gondii after peroral ingestion of tissue cyst forming strain (Me49 strain) in mice. Korean J Parasitol. 1999; 37(3): 157-62[PubMed]
  • 26. Labesse G, Gelin M, Bessin Y, Lebrun M, Papoin J, Cerdan R, et al. ROP2 from Toxoplasma gondii: a virulence factor with a protein-kinase fold and no enzymatic activity. Structure. 2009; 17(1): 139-46[DOI][PubMed]
  • 27. Ahn HJ, Kim S, Nam HW. Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii. Korean J Parasitol. 2006; 44(3): 251-4[PubMed]
  • 28. Bessieres MH, Le Breton S, Seguela JP. Analysis by immunoblotting of Toxoplasma gondii exo-antigens and comparison with somatic antigens. Parasitol Res. 1992; 78(3): 222-8[PubMed]
  • 29. Ahn MH, Son HJ, Leem MH, Min DY. [Antigen analysis of Toxoplasma gondii lysate and excretory-secretory materials by enzyme-linked immunoelectrotransfer blot (EITB)]. Korean J Parasitol. 1994; 32(4): 249-57[PubMed]
  • 30. Dedkova LM, Gladkova SV, Bormotov NI, Tkachenko TN, Belanov EF. [Investigation of the protein composition and immunochemical properties of Toxoplasma gondii excretory and secretory antigen]. Med Parazitol (Mosk). 2000; (1): 20-4[PubMed]
  • 31. Bradley PJ, Ward C, Cheng SJ, Alexander DL, Coller S, Coombs GH, et al. Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii. J Biol Chem. 2005; 280(40): 34245-58[DOI][PubMed]
  • 32. Son ES, Nam HW. Detection and characterization of excretory/secretory proteins from Toxoplasma gondii by monoclonal antibodies. Korean J Parasitol. 2001; 39(1): 49-56[PubMed]
  • 33. Mevelec MN, Mercereau-Puijalon O, Buzoni-Gatel D, Bourguin I, Chardes T, Dubremetz JF, et al. Mapping of B epitopes in GRA4, a dense granule antigen of Toxoplasma gondii and protection studies using recombinant proteins administered by the oral route. Parasite Immunol. 1998; 20(4): 183-95[PubMed]
  • 34. Ahn HJ, Kim S, Nam HW. Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii. Biochem Biophys Res Commun. 2005; 331(2): 614-20[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments