Jundishapur Journal of Microbiology

Published by: Kowsar

Molecular Characterization of Cryptosporidium spp. Isolated From Immunocompromised Patients and Children

Abdollah Rafiei 1 , 2 , * , Zahra Rashno 1 , Alireza Samarbafzadeh 3 and Shahram Khademvatan 1
Authors Information
1 Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 Department of Medical Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: April 01, 2014, 7 (4); e9183
  • Published Online: April 1, 2014
  • Article Type: Research Article
  • Received: November 19, 2012
  • Revised: January 29, 2013
  • Accepted: July 27, 2013
  • DOI: 10.5812/jjm.9183

To Cite: Rafiei A, Rashno Z, Samarbafzadeh A, Khademvatan S. Molecular Characterization of Cryptosporidium spp. Isolated From Immunocompromised Patients and Children, Jundishapur J Microbiol. 2014 ; 7(4):e9183. doi: 10.5812/jjm.9183.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Xiao L, Ryan UM. Cryptosporidium and cryptosporidiosis. 2012; : 119–171
  • 2. Hunter PR, Hughes S, Woodhouse S, Raj N, Syed Q, Chalmers RM, et al. Health sequelae of human cryptosporidiosis in immunocompetent patients. Clin Infect Dis. 2004; 39(4): 504-10[DOI][PubMed]
  • 3. Gentile G, Venditti M, Micozzi A, Caprioli A, Donelli G, Tirindelli C, et al. Cryptosporidiosis in patients with hematologic malignancies. Rev Infect Dis. 1991; 13(5): 842-6[PubMed]
  • 4. Tanyuksel M, Gun H, Doganci L. Prevalence of Cryptosporidium sp. in patients with neoplasia and diarrhea. Scand J Infect Dis. 1995; 27(1): 69-70[PubMed]
  • 5. Sreedharan A, Jayshree RS, Sridhar H. Cryptosporidiosis among cancer patients: an observation. J Diarrhoeal Dis Res. 1996; 14(3): 211-3[PubMed]
  • 6. Yang W, Chen P, Villegas EN, Landy RB, Kanetsky C, Cama V, et al. Cryptosporidium source tracking in the Potomac River watershed. Appl Environ Microbiol. 2008; 74(21): 6495-504[DOI][PubMed]
  • 7. Morgan UM, Deplazes P, Forbes DA, Spano F, Hertzberg H, Sargent KD, et al. Sequence and PCR-RFLP analysis of the internal transcribed spacers of the rDNA repeat unit in isolates of Cryptosporidium from different hosts. Parasitology. 1999; 118 ( Pt 1): 49-58[PubMed]
  • 8. Plutzer J, Karanis P. Genetic polymorphism in Cryptosporidium species: an update. Vet Parasitol. 2009; 165(3-4): 187-99[DOI][PubMed]
  • 9. Xiao L, Zhou L, Santin M, Yang W, Fayer R. Distribution of Cryptosporidium parvum subtypes in calves in eastern United States. Parasitol Res. 2007; 100(4): 701-6[DOI][PubMed]
  • 10. Cama VA, Ross JM, Crawford S, Kawai V, Chavez-Valdez R, Vargas D, et al. Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J Infect Dis. 2007; 196(5): 684-91[DOI][PubMed]
  • 11. Robinson G, Elwin K, Chalmers RM. Unusual Cryptosporidium genotypes in human cases of diarrhea. Emerg Infect Dis. 2008; 14(11): 1800-2[DOI][PubMed]
  • 12. Feng Y, Ortega Y, He G, Das P, Xu M, Zhang X, et al. Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Vet Parasitol. 2007; 144(1-2): 1-9[DOI][PubMed]
  • 13. Morgan UM, Xiao L, Fayer R, Lal AA, Thompson RC. Epidemiology and strain variation of Cryptosporidium parvum. Contrib Microbiol. 2000; 6: 116-39[PubMed]
  • 14. Xiao L, Fayer R, Ryan U, Upton SJ. Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev. 2004; 17(1): 72-97[PubMed]
  • 15. Fayer R, Santin M, Trout JM, Greiner E. Prevalence of species and genotypes of Cryptosporidium found in 1-2-year-old dairy cattle in the eastern United States. Vet Parasitol. 2006; 135(2): 105-12[DOI][PubMed]
  • 16. Xiao L, Fayer R, Ryan U, Upton SJ. Response to the newly proposed species Cryptosporidium pestis. Trends Parasitol. 2007; 23(2): 41-3[DOI][PubMed]
  • 17. Yosefi F, Rahdar M, Alavi S, Samany A. A study on Prevalence of Gastrointestinal Parasitic Infections in HIV (+) Patients Referred to Ahvaz Razi Hospital in 2008-2009. Jundishapur J Microbiol. 2012; : 424-6[DOI]
  • 18. Coupe S, Sarfati C, Hamane S, Derouin F. Detection of cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. J Clin Microbiol. 2005; 43(3): 1017-23[DOI][PubMed]
  • 19. Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, et al. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol. 1999; 65(4): 1578-83[PubMed]
  • 20. Limor JR, Lal AA, Xiao L. Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. J Clin Microbiol. 2002; 40(7): 2335-8[PubMed]
  • 21. Park JH, Guk SM, Han ET, Shin EH, Kim JL, Chai JY. Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea. Korean J Parasitol. 2006; 44(1): 27-33[PubMed]
  • 22. Schindler AR, Abs El-Osta YG, Stevens M, Sinclair MI, Gasser RB. Capillary electrophoretic analysis of fragment length polymorphism in ribosomal markers of Cryptosporidium from humans. Mol Cell Probes. 2005; 19(6): 394-9[DOI][PubMed]
  • 23. Xiao L. Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol. 2010; 124(1): 80-9[DOI][PubMed]
  • 24. Xiao L, Bern C, Sulaiman IM, Lal AA. Cryptosporidium: From Molecules to Disease: From Molecules to Disease. 2004; : 121–146
  • 25. Dehkordy AB, Rafiei A, Alavi S, Latifi S. Prevalence of cryptosporidium infection in immunocompromised patients, in South-west of iran, 2009-10. Iran J Parasitol. 2010; 5(4): 42-7[PubMed]
  • 26. Meamar AR, Guyot K, Certad G, Dei-Cas E, Mohraz M, Mohebali M, et al. Molecular characterization of Cryptosporidium isolates from humans and animals in Iran. Appl Environ Microbiol. 2007; 73(3): 1033-5[DOI][PubMed]
  • 27. Pirestani M, Sadraei J, Dalimi Asl A, Zavvar M, Vaeznia H. Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran. Parasitol Res. 2008; 103(2): 467-72[DOI][PubMed]
  • 28. Zavvar M, Sadraei J, Emadi H, Pirestani M. The use of a nested PCR-RFLP technique, based on the parasite's 18S ribosomal RNA, to characterise Cryptosporidium isolates from HIV/AIDS patients. Ann Trop Med Parasitol. 2008; 102(7): 597-601[DOI][PubMed]
  • 29. Al-Brikan FA, Salem HS, Beeching N, Hilal N. Multilocus genetic analysis of Cryptosporidium isolates from Saudi Arabia. J Egypt Soc Parasitol. 2008; 38(2): 645-58[PubMed]
  • 30. Iqbal J, Khalid N, Hira PR. Cryptosporidiosis in Kuwaiti children: association of clinical characteristics with Cryptosporidium species and subtypes. J Med Microbiol. 2011; 60: 647-52[DOI][PubMed]
  • 31. Matos O, Alves M, Xiao L, Cama V, Antunes F. Cryptosporidium felis and C. meleagridis in persons with HIV, Portugal. Emerg Infect Dis. 2004; 10(12): 2256-7[DOI][PubMed]
  • 32. Muthusamy D, Rao SS, Ramani S, Monica B, Banerjee I, Abraham OC, et al. Multilocus genotyping of Cryptosporidium sp. isolates from human immunodeficiency virus-infected individuals in South India. J Clin Microbiol. 2006; 44(2): 632-4[DOI][PubMed]
  • 33. Pedraza-Diaz S, Amar CF, McLauchlin J, Nichols GL, Cotton KM, Godwin P, et al. Cryptosporidium meleagridis from humans: molecular analysis and description of affected patients. J Infect. 2001; 42(4): 243-50[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments