Jundishapur Journal of Microbiology

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Determination of Vancomycin Resistant Enterococcus faecium Diversity in Tehran Sewage Using Plasmid Profile, Biochemical Fingerprinting and Antibiotic Resistance

Katayoun Borhani 1 , Ali Ahmadi 2 , Fateh Rahimi 3 , Mohammad Reza Pourshafie 1 , * and Malihe Talebi 2 , *
Authors Information
1 Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran
2 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran
Corresponding Authors:
Article information
  • Jundishapur Journal of Microbiology: February 01, 2014, 7 (2); e8951
  • Published Online: February 1, 2014
  • Article Type: Research Article
  • Received: November 4, 2012
  • Revised: January 23, 2013
  • Accepted: February 19, 2013
  • DOI: 10.5812/jjm.8951

To Cite: Borhani K, Ahmadi A, Rahimi F, Pourshafie M R, Talebi M. Determination of Vancomycin Resistant Enterococcus faecium Diversity in Tehran Sewage Using Plasmid Profile, Biochemical Fingerprinting and Antibiotic Resistance, Jundishapur J Microbiol. 2014 ; 7(2):e8951. doi: 10.5812/jjm.8951.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Godfree Alan, Farrell Joseph. Processes for Managing Pathogens. J Environ Quality. 2005; 34(1): 105[DOI]
  • 2. Xu J, Gallert C, Winter J. Multiple antibiotic resistances of Enterococcus isolates from raw or sand-filtered sewage. Appl Microbiol Biotechnol. 2007; 74(2): 493-500[DOI][PubMed]
  • 3. Kuhn I, Iversen A, Finn M, Greko C, Burman LG, Blanch AR, et al. Occurrence and relatedness of vancomycin-resistant enterococci in animals, humans, and the environment in different European regions. Appl Environ Microbiol. 2005; 71(9): 5383-90[DOI][PubMed]
  • 4. Cordeiro JC, Silbert S, Reis AO, Sader HS. Inter-hospital dissemination of glycopeptide-resistant Enterococcus faecalis in Brazil. Clin Microbiol Infect. 2004; 10(3): 260-2[PubMed]
  • 5. Kawalec M, Gniadkowski M, Zaleska M, Ozorowski T, Konopka L, Hryniewicz W. Outbreak of vancomycin-resistant Enterococcus faecium of the phenotype VanB in a hospital in Warsaw, Poland: probable transmission of the resistance determinants into an endemic vancomycin-susceptible strain. J Clin Microbiol. 2001; 39(5): 1781-7[DOI][PubMed]
  • 6. Klare Ingo, Konstabel Carola, Badstübner Dietlinde, Werner Guido, Witte Wolfgang. Occurrence and spread of antibiotic resistances in Enterococcus faecium. Int J Food Microbiol. 2003; 88(2-3): 269-290[DOI]
  • 7. Gallert C, Fund K, Winter J. Antibiotic resistance of bacteria in raw and biologically treated sewage and in groundwater below leaking sewers. Appl Microbiol Biotechnol. 2005; 69(1): 106-12[DOI][PubMed]
  • 8. Singh A, Goering RV, Simjee S, Foley SL, Zervos MJ. Application of molecular techniques to the study of hospital infection. Clin Microbiol Rev. 2006; 19(3): 512-30[DOI][PubMed]
  • 9. Talebi M, Rahimi F, Katouli M, Kühn I, Möllby R, Eshraghi S, et al. Prevalence and Antimicrobial Resistance of Enterococcal Species in Sewage Treatment Plants in Iran. Water, Air, and Soil Pollution. 2007; 185(1-4): 111-119[DOI]
  • 10. Wikler Matthew A. Performance Standards for Antimicrobial Susceptibility Testing: Twentieth Informational Supplement. 2010;
  • 11. Kariyama Reiko, Mitsuhata Ritsuko, Chow Joseph W, Clewell Don B, Kumon Hiromi. Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci. J Clin Microbiol. 2000; 38(8): 3092-3095
  • 12. Kühn Inger, Iversen Aina, Möllby Roland. The PhenePlate™ system for studies of the diversity of enterococcal populations from the food chain and the environment. Int J Food Microbiol. 2003; 88(2-3): 189-196[DOI]
  • 13. Amyes Sebastian GB. Enterococci and streptococci. Int J Antimicrob Agents. 2007; 29[DOI]
  • 14. Iversen A, Kuhn I, Franklin A, Mollby R. High prevalence of vancomycin-resistant enterococci in Swedish sewage. Appl Environ Microbiol. 2002; 68(6): 2838-42[PubMed]
  • 15. Sahlstrom L, Rehbinder V, Albihn A, Aspan A, Bengtsson B. Vancomycin resistant enterococci (VRE) in Swedish sewage sludge. Acta Vet Scand. 2009; 51: 24[DOI][PubMed]
  • 16. van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect. 2007; 13 Suppl 3: 1-46[DOI][PubMed]
  • 17. Blanch AR, Caplin JL, Iversen A, Kohn I, Manero A, Taylor HD, et al. Comparison of enterococcal populations related to urban and hospital wastewater in various climatic and geographic European regions. J Appl Microbiol. 2003; 94(6): 994-1002[DOI]
  • 18. Franz CM, Muscholl-Silberhorn AB, Yousif NM, Vancanneyt M, Swings J, Holzapfel WH. Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl Environ Microbiol. 2001; 67(9): 4385-9[PubMed]
  • 19. Zheng B, Tomita H, Xiao YH, Wang S, Li Y, Ike Y. Molecular characterization of vancomycin-resistant enterococcus faecium isolates from mainland China. J Clin Microbiol. 2007; 45(9): 2813-8[DOI][PubMed]
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