Jundishapur Journal of Microbiology

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Identification of Klebsiella pneumoniae K1 and K2 Capsular Types by PCR and Quellung Test

Mohammad Mehdi Feizabadi 1 , Nafiseh Raji 1 , * and Somaieh Delfani 1
Authors Information
1 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2013, 6 (9); e7585
  • Published Online: November 1, 2013
  • Article Type: Research Article
  • Received: August 29, 2012
  • Revised: November 5, 2012
  • Accepted: December 15, 2012
  • DOI: 10.5812/jjm.7585

To Cite: Feizabadi M M, Raji N, Delfani S. Identification of Klebsiella pneumoniae K1 and K2 Capsular Types by PCR and Quellung Test, Jundishapur J Microbiol. 2013 ; 6(9):e7585. doi: 10.5812/jjm.7585.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998; 11(4): 589-603[PubMed]
  • 2. Turton JF, Englender H, Gabriel SN, Turton SE, Kaufmann ME, Pitt TL. Genetically similar isolates of Klebsiella pneumoniae serotype K1 causing liver abscesses in three continents. J Med Microbiol. 2007; 56: 593-7[DOI][PubMed]
  • 3. Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, et al. Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization. PLoS One. 2009; 4(3)[DOI][PubMed]
  • 4. Lau HY, Clegg S, Moore TA. Identification of Klebsiella pneumoniae genes uniquely expressed in a strain virulent using a murine model of bacterial pneumonia. Microb Pathog. 2007; 42(4): 148-55[DOI][PubMed]
  • 5. Yu VL, Hansen DS, Ko WC, Sagnimeni A, Klugman KP, von Gottberg A, et al. Virulence characteristics of Klebsiella and clinical manifestations of K. pneumoniae bloodstream infections. Emerg Infect Dis. 2007; 13(7): 986-93[DOI][PubMed]
  • 6. Diancourt L, Passet V, Verhoef J, Grimont PA, Brisse S. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J Clin Microbiol. 2005; 43(8): 4178-82[DOI][PubMed]
  • 7. Turton JF, Baklan H, Siu LK, Kaufmann ME, Pitt TL. Evaluation of a multiplex PCR for detection of serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes. FEMS Microbiol Lett. 2008; 284(2): 247-52[DOI][PubMed]
  • 8. Fang CT, Lai SY, Yi WC, Hsueh PR, Liu KL, Chang SC. Klebsiella pneumoniae genotype K1: an emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess. Clin Infect Dis. 2007; 45(3): 284-93[DOI][PubMed]
  • 9. Yu WL, Fung CP, Ko WC, Cheng KC, Lee CC, Chuang YC. Polymerase chain reaction analysis for detecting capsule serotypes K1 and K2 of Klebsiella pneumoniae causing abscesses of the liver and other sites. J Infect Dis. 2007; 195(8): 1235-6[DOI][PubMed]
  • 10. Chuang YP, Fang CT, Lai SY, Chang SC, Wang JT. Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis. 2006; 193(5): 645-54[DOI][PubMed]
  • 11. Cryz SJ, Jr, Mortimer PM, Mansfield V, Germanier R. Seroepidemiology of Klebsiella bacteremic isolates and implications for vaccine development. J Clin Microbiol. 1986; 23(4): 687-90[PubMed]
  • 12. Grangeasse C, Doublet P, Cozzone AJ. Tyrosine phosphorylation of protein kinase Wzc from Escherichia coli K12 occurs through a two-step process. J Biol Chem. 2002; 277(9): 7127-35[DOI][PubMed]
  • 13. Feizabadi MM, Delfani S, Raji N, Majnooni A, Aligholi M, Shahcheraghi F, et al. Distribution of bla(TEM), bla(SHV), bla(CTX-M) genes among clinical isolates of Klebsiella pneumoniae at Labbafinejad Hospital, Tehran, Iran. Microb Drug Resist. 2010; 16(1): 49-53[DOI][PubMed]
  • 14. Edmondson AS, Cooke EM. The production of antisera to the Klebsiella capsular antigens. J Appl Bacteriol. 1979; 46(3): 579-84[PubMed]
  • 15. Liao CH, Huang YT, Lai CC, Chang CY, Chu FY, Hsu MS, et al. Klebsiella pneumoniae bacteremia and capsular serotypes, Taiwan. Emerg Infect Dis. 2011; 17(6): 1113-5[DOI][PubMed]
  • 16. Pan YJ, Fang HC, Yang HC, Lin TL, Hsieh PF, Tsai FC, et al. Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype. J Clin Microbiol. 2008; 46(7): 2231-40[DOI][PubMed]
  • 17. Vincent C, Doublet P, Grangeasse C, Vaganay E, Cozzone AJ, Duclos B. Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb. J Bacteriol. 1999; 181(11): 3472-7[PubMed]
  • 18. Gierczynski R, Jagielski M, Rastawicki W, Kaluzewski S. Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying the cps loci for K1 and K2 capsule biosynthesis. Pol J Microbiol. 2007; 56(3): 153-6[PubMed]
  • 19. Brisse S, Issenhuth-Jeanjean S, Grimont PA. Molecular serotyping of Klebsiella species isolates by restriction of the amplified capsular antigen gene cluster. J Clin Microbiol. 2004; 42(8): 3388-98[DOI][PubMed]
  • 20. Struve C, Bojer M, Nielsen EM, Hansen DS, Krogfelt KA. Investigation of the putative virulence gene magA in a worldwide collection of 495 Klebsiella isolates: magA is restricted to the gene cluster of Klebsiella pneumoniae capsule serotype K1. J Med Microbiol. 2005; 54: 1111-3[DOI][PubMed]
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