Jundishapur Journal of Microbiology

Published by: Kowsar

Detection of Toxoplasmosis in Renal Transplant Recipients by ELISA and PCR Methods in Ahvaz, South-West of Iran

Shahrzad Soltani 1 , Shahram Khademvatan 1 , 2 , Jasem Saki 1 , 3 , * and Heshmatollah Shahbazian 4
Authors Information
1 Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Science, Ahvaz, IR Iran
4 Department of Nephrology, Golestan Hospital and Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2013, 6 (9); e7642
  • Published Online: November 1, 2013
  • Article Type: Research Article
  • Received: August 7, 2012
  • Revised: November 12, 2012
  • Accepted: December 15, 2012
  • DOI: 10.5812/jjm.7642

To Cite: Soltani S, Khademvatan S, Saki J, Shahbazian H. Detection of Toxoplasmosis in Renal Transplant Recipients by ELISA and PCR Methods in Ahvaz, South-West of Iran, Jundishapur J Microbiol. 2013 ; 6(9):e7642. doi: 10.5812/jjm.7642.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Montoya JG, Liesenfeld O. Toxoplasmosis. Lancet. 2004; 363(9425): 1965-76[DOI][PubMed]
  • 2. Gopal K, Tripathy SS, Bersillon JL, Dubey SP. Chlorination byproducts, their toxicodynamics and removal from drinking water. J Hazard Mater. 2007; 140(1-2): 1-6[DOI][PubMed]
  • 3. Dubey JP, Beattie CP. Toxoplasmosis of Animals and Man. 1988; : 1-220
  • 4. Galvan-Ramirez ML, Guillen-Vargas C, Saavedra-Duran R, Islos-Rodriguez A. Analysis of Toxoplasma gondii antigens with sera from toxoplasmosis patients. Rev Soc Bras Med Trop. 1998; 31(3): 271-7[PubMed]
  • 5. Hazrati Tappeh KH, Gharavi MJ, Makhdoumi K, Rahbar M, Taghizadeh A. Prevalence of Cryptosporidium spp. Infection in Renal Transplant and Hemodialysis Patients. Iranian J Publ Health. 2006; 35(3)
  • 6. Mariuz P, Bosler EM, Luft BJ. Toxoplasmosis in individuals with AIDS. Infect Dis Clin North Am. 1994; 8(2): 365-81[PubMed]
  • 7. Holliman RE, Johnson JD, Adams S, Pepper JR. Toxoplasmosis and heart transplantation. J Heart Lung Transplant. 1991; 10(4): 608-10[PubMed]
  • 8. Renoult E, Chabot F, Aymard B, Hestin D, Delorme N, Biava MF, et al. Generalized toxoplasmosis in two renal transplant recipients who received a kidney from the same donor. Rev Infect Dis. 1991; 13(1): 180-1[PubMed]
  • 9. Israelski DM, Remington JS. Toxoplasmosis in the non-AIDS immunocompromised host. Curr Clin Top Infect Dis. 1993; 13: 322-56[PubMed]
  • 10. Renoult E, Georges E, Biava MF, Hulin C, Frimat L, Hestin D, et al. Toxoplasmosis in kidney transplant recipients: a life-threatening but treatable disease. Transplant Proc. 1997; 29(1-2): 821-2[PubMed]
  • 11. Lucan M. [Textbook of Surgical Urologic Techniques]. 2001;
  • 12. Novick AC. Surgery of Renal Transplantation and Complications. Vascular Problems in Urologic Surgery. 1983;
  • 13. Wulf MW, van Crevel R, Portier R, Ter Meulen CG, Melchers WJ, van der Ven A, et al. Toxoplasmosis after renal transplantation: implications of a missed diagnosis. J Clin Microbiol. 2005; 43(7): 3544-7[DOI][PubMed]
  • 14. Hebraud B, Kamar N, Borde JS, Bessieres MH, Galinier M, Rostaing L. Unusual presentation of primary toxoplasmosis infection in a kidney-transplant patient complicated by an acute left-ventricular failure. NDT Plus. 2008; 1(6): 429-432[DOI][PubMed]
  • 15. Renoult E, Georges E, Biava MF, Hulin C, Frimat L, Hestin D, et al. Toxoplasmosis in kidney transplant recipients: report of six cases and review. Clin Infect Dis. 1997; 24(4): 625-34[PubMed]
  • 16. Luft BJ, Remington JS. Toxoplasmic encephalitis in AIDS. Clin Infect Dis. 1992; 15(2): 211-22[PubMed]
  • 17. Weenink JJ, Weenink AG, Geerlings SE, van Gool T, Bemelman FJ. Severe cerebral toxoplasma infection cannot be excluded by a normal CT scan. Neth J Med. 2009; 67(4): 150-2[PubMed]
  • 18. Petersen E, Borobio MV, Guy E, Liesenfeld O, Meroni V, Naessens A, et al. European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol. 2005; 43(4): 1570-4[DOI][PubMed]
  • 19. Lamoril J, Molina JM, de Gouvello A, Garin YJ, Deybach JC, Modai J, et al. Detection by PCR of Toxoplasma gondii in blood in the diagnosis of cerebral toxoplasmosis in patients with AIDS. J Clin Pathol. 1996; 49(1): 89-92[PubMed]
  • 20. Fazaeli A, Carter PE, Darde ML, Pennington TH. Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis. Int J Parasitol. 2000; 30(5): 637-42[PubMed]
  • 21. Nissapatorn V, Kamarulzaman A, Init I, Tan LH, Rohela M, Norliza A, et al. Seroepidemiology of toxoplasmosis among HIV-infected patients and healthy blood donors. Med J Malaysia. 2002; 57(3): 304-10[PubMed]
  • 22. Cervera C, Moreno A. [Infections in recipients of a kidney-pancreas transplant]. Enferm Infecc Microbiol Clin. 2007; 25(5): 345-55[PubMed]
  • 23. Sukthana Y, Chintana T, Damrongkitchaiporn S, Lekkla A. Serological study of Toxoplasma gondii in kidney recipients. J Med Assoc Thai. 2001; 84(8): 1137-41[PubMed]
  • 24. Wallace MR, Rossetti RJ, Olson PE. Cats and toxoplasmosis risk in HIV-infected adults. JAMA. 1993; 269(1): 76-7[PubMed]
  • 25. Magaldi C, Elkis H, Pattoli D, Coscina AL. Epidemic of toxoplasmosis at a university in Sao-Jose-dos Campos, S.P. Brazil. 1. Clinical and serologic data. Rev Latinoam Microbiol Parasitol (Mex). 1969; 11(1): 5-13[PubMed]
  • 26. Choi WY, Nam HW, Kwak NH, Huh W, Kim YR, Kang MW, et al. Foodborne outbreaks of human toxoplasmosis. J Infect Dis. 1997; 175(5): 1280-2[PubMed]
  • 27. Lin YL, Liao YS, Liao LR, Chen FN, Kuo HM, He S. Seroprevalence and sources of Toxoplasma infection among indigenous and immigrant pregnant women in Taiwan. Parasitol Res. 2008; 103(1): 67-74[DOI][PubMed]
  • 28. Remington JS, Thulliez P, Montoya JG. Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol. 2004; 42(3): 941-5[PubMed]
  • 29. Liesenfeld O, Press C, Montoya JG, Gill R, Isaac-Renton JL, Hedman K, et al. False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the Platelia Toxo IgM test. J Clin Microbiol. 1997; 35(1): 174-8[PubMed]
  • 30. Iqbal J, Nampoory MR, Johnv KV, Khalid N, Al-Mousawi M. Determination of antibodies to Toxoplasma gondii and CMV in renal transplant recipients. Transplant Proc. 2003; 35(7): 2703-5[PubMed]
  • 31. Holliman R, Johnson J, Savva D, Cary N, Wreghitt T. Diagnosis of toxoplasma infection in cardiac transplant recipients using the polymerase chain reaction. J Clin Pathol. 1992; 45(10): 931-2[PubMed]
  • 32. Chabbert E, Lachaud L, Crobu L, Bastien P. Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues. J Clin Microbiol. 2004; 42(4): 1719-22[PubMed]
  • 33. Gharavi MJ. Prevalence and diagnosis of Toxoplasmosis in immunodeficient patients. Abstract book of 2nd National Congress of Parasitic Diseases. 1997;
  • 34. Gharavi MJ, Jalali S, Khademvatan S, Heydari S. Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: comparison of pre- and post-transplantation status. Ann Trop Med Parasitol. 2011; 105(5): 367-71[DOI][PubMed]
  • 35. Nateghi Rostami M, Eskandari E, Garoosi Z, Mohajeri N, Rezaian M, Keshavarz H. Serological Study of Toxoplasma gondii Infection Using IFA Method in Renal Transplant Recipients. Iranian J Parasitol. 2006; 1(1)
  • 36. Garedaghi Y, Safarmashaei S. Survey of Toxoplasma Contamination in Kidney Recipient Patients by ELISA Method and Comparison it with Control Group in Tabriz (East-azerbaijan), Iran. Adv Environ Biol. 2011; 5(4): 769-772
  • 37. Nissapatorn V, Leong TH, Lee R, Init Ithoi, Ibrahim J, Yen TS. Seroepidemiology of toxoplasmosis in renal patients. Southeast Asian J Trop Med Public Health. 2011; 42(2): 237-47[PubMed]
  • 38. Aubert D, Foudrinier F, Villena I, Pinon JM, Biava MF, Renoult E. PCR for diagnosis and follow-up of two cases of disseminated toxoplasmosis after kidney grafting. J Clin Microbiol. 1996; 34(5): 1347[PubMed]
  • 39. Homan WL, Vercammen M, De Braekeleer J, Verschueren H. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol. 2000; 30(1): 69-75[PubMed]
  • 40. Guo ZG, Gross U, Johnson AM. Toxoplasma gondii virulence markers identified by random amplified polymorphic DNA polymerase chain reaction. Parasitol Res. 1997; 83(5): 458-63[PubMed]
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