Jundishapur Journal of Microbiology

Published by: Kowsar

Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR

Mehdi Zarei 1 , * , Narjes Basiri 2 , Amirhesam Jamnejad 1 and Mohammad Hadi Eskandari 3
Authors Information
1 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
2 Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, IR Iran
3 Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: October 01, 2013, 6 (8); e7244
  • Published Online: October 1, 2013
  • Article Type: Research Article
  • Received: July 15, 2012
  • Revised: October 28, 2012
  • Accepted: November 10, 2012
  • DOI: 10.5812/jjm.7244

To Cite: Zarei M, Basiri N, Jamnejad A, Eskandari M H. Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR, Jundishapur J Microbiol. 2013 ; 6(8):e7244. doi: 10.5812/jjm.7244.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Bhagwat AA. Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR. Int J Food Microbiol. 2003; 84(2): 217-24[PubMed]
  • 2. Germini Andrea, Masola Annalisa, Carnevali Paola, Marchelli Rosangela. Simultaneous detection of Escherichia coli O175: H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR. Food Cont. 2009; 20(8): 733-738
  • 3. European Commission. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Off J Eur Union L. 2005; 338(1)
  • 4. Karami Ali, Ahmadi Zeynab, Safiri Zahra, Pourali Fateme. Detection of Salmonella strain by rapid-cycle multiplex PCR. Jundishapur J Microbiol. 2012; 4(2): 91-8
  • 5. Kim JS, Lee GG, Park JS, Jung YH, Kwak HS, Kim SB, et al. A novel multiplex PCR assay for rapid and simultaneous detection of five pathogenic bacteria: Escherichia coli O157:H7, Salmonella, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticus. J Food Prot. 2007; 70(7): 1656-62[PubMed]
  • 6. Kahraman T, Aydin A. [Prevalence of Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in meat and meat products in Turkey]. Arch Lebensmittelhyg. 2009; 60: 6-11
  • 7. Minami Atsuka, Chaicumpa Wanpen, Chongsa-Nguan Manas, Samosornsuk Seksun, Monden Shuko, Takeshi Kouichi, et al. Prevalence of foodborne pathogens in open markets and supermarkets in Thailand. Food Control. 2010; 21(3): 221-226
  • 8. Osaili Tareq M, Alaboudi Akram R, Nesiar Ehab A. Prevalence of Listeria spp. and antibiotic susceptibility of Listeria monocytogenes isolated from raw chicken and ready-to-eat chicken products in Jordan. Food Control. 2011; 22(3): 586-590
  • 9. Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991; 13: 60-98[PubMed]
  • 10. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1): 142-201[PubMed]
  • 11. Zheng H, Jing H, Wang H, Xia S, Hu W, Cui S, et al. stx2vha is the dominant genotype of Shiga toxin-producing Escherichia coli O157:H7 isolated from patients and domestic animals in three regions of China. Microbiol Immunol. 2005; 49(12): 1019-26[PubMed]
  • 12. Jalali M, Abedi D. Prevalence of Listeria species in food products in Isfahan, Iran. Int J Food Microbiol. 2008; 122(3): 336-40[DOI][PubMed]
  • 13. Rouquette C, Berche P. The pathogenesis of infection by Listeria monocytogenes. Microbiologia. 1996; 12(2): 245-58[PubMed]
  • 14. Madden Robert H, Espie WEric, Moran Lynn, McBride Jane, Scates Pam. Occurrence of Escherichia coli O157: H7, Listeria monocytogenes,Salmonella and Campylobacter spp. on beef carcasses in Northern Ireland. Meat science. 2001; 58(4): 343-346
  • 15. Schlech WF, 3rd. Foodborne listeriosis. Clin Infect Dis. 2000; 31(3): 770-5[DOI][PubMed]
  • 16. Lammerding AM, Garcia MM, Mann ED, Robinson Y, Dorward WJ, Truscott RB, et al. Prevalence of Salmonella and thermophilic Campylobacter in fresh pork, beef, veal and poultry in Canada. J Food Protect. 1988; 51
  • 17. D’Aoust JY, Maurer J, Bailey JS, Doyle MP, Beuchat LR, Montville TJ. Food Microbiology: fundamentals and frontiers. 2001; : 141-178
  • 18. Breum SO, Boel J. Prevalence of Escherichia coli O157 and verocytotoxin producing E. coli (VTEC) on Danish beef carcasses. Int J Food Microbiol. 2010; 141(1-2): 90-6[DOI][PubMed]
  • 19. Chapman PA, Cerdan Malo AT, Ellin M, Ashton R. Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK. Int J Food Microbiol. 2001; 64(1-2): 139-50[PubMed]
  • 20. Kalchayanand N, Arthur TM, Bosilevac JM, Brichta-Harhay DM, Guerini MN, Shackelford SD, et al. Microbiological characterization of lamb carcasses at commercial processing plants in the United States. J Food Prot. 2007; 70(8): 1811-9[PubMed]
  • 21. Battisti A, Lovari S, Franco A, Di Egidio A, Tozzoli R, Caprioli A, et al. Prevalence of Escherichia coli O157 in lambs at slaughter in Rome, central Italy. Epidemiol Infect. 2006; 134(2): 415-9[DOI][PubMed]
  • 22. Rocourt J, Cossart P. Food Microbiology-Fundamentals and Frontiers;. 1997; : 337-352
  • 23. Zarei M, Maktabi S, Ghorbanpour M. Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in seafood products using multiplex polymerase chain reaction. Foodborne Pathog Dis. 2011; 9(2): 108-12[DOI][PubMed]
  • 24. Li Qiongzhen, Sherwood Julie S, Logue Catherine M. The prevalence of Listeria, Salmonell, scherichia coli and E. coli O157: H7 on bison carcasses during processing. Food Microbiol. 2004; 21(6): 791-799
  • 25. Little CL, Richardson JF, Owen RJ, de Pinna E, Threlfall EJ. Campylobacter and Salmonella in raw red meats in the United Kingdom: prevalence, characterization and antimicrobial resistance pattern, 2003-2005. Food Microbiol. 2008; 25(3): 538-43[DOI][PubMed]
  • 26. Busani L, Cigliano A, Taioli E, Caligiuri V, Chiavacci L, Di Bella C, et al. Prevalence of Salmonella enterica and Listeria monocytogenes contamination in foods of animal origin in Italy. J Food Prot. 2005; 68(8): 1729-33[PubMed]
  • 27. Zhao C, Ge B, De Villena J, Sudler R, Yeh E, Zhao S, et al. Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., area. Appl Environ Microbiol. 2001; 67(12): 5431-6[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments