Jundishapur Journal of Microbiology

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Molecular Typing of Brucella melitensis and B. abortus From Human Blood Samples Using PCR-RFLP Method

Reza Mirnejad 1 , Mozafar Mohammadi 1 , Ali Majdi 2 , Niloufar Taghizoghi 3 and Vahhab Piranfar 4 , *
Authors Information
1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Young Researchers Club, Tonekabon Branch, Islamic Azad University, Tonekabon, IR Iran
3 Bounty house, Stowage, Bellerbys College, London, United Kingdom
4 Department of Biology, Tonekabon Branch, Islamic Azad university of Tonekabon, Tonekabon, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 01, 2013, 6 (6); e7197
  • Published Online: August 1, 2013
  • Article Type: Research Article
  • Received: July 11, 2012
  • Revised: December 31, 2012
  • Accepted: January 27, 2013
  • DOI: 10.5812/jjm.7197

To Cite: Mirnejad R, Mohammadi M, Majdi A, Taghizoghi N, Piranfar V. Molecular Typing of Brucella melitensis and B. abortus From Human Blood Samples Using PCR-RFLP Method, Jundishapur J Microbiol. 2013 ; 6(6):e7197. doi: 10.5812/jjm.7197.

Abstract
Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Cutler SJ, Whatmore AM, Commander NJ. Brucellosis--new aspects of an old disease. J Appl Microbiol. 2005; 98(6): 1270-81[DOI][PubMed]
  • 2. Christopher S, Umapathy BL, Ravikumar KL. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. J Lab Physicians. 2010; 2(2): 55-60[DOI][PubMed]
  • 3. Guerra H. The brucellae and their success as pathogens. Crit Rev Microbiol. 2007; 33(4): 325-31[DOI][PubMed]
  • 4. Davar SS, Reza AM, Sahar K, Mehdi SS, Arfa M. Biological and immunological characteristics of Brucella abortus S99 major outer membrane proteins. Jundishapur J Microbiol. 2012; 4(1)
  • 5. Shapouri R, Rahnema M. Evaluation of antimicrobial effect of hops extracts on intramacrophages Brucella abortus and B. melitensis. Jundishapur J Microbiol. 2011; 4(2)
  • 6. Seimenis A, Morelli D, Mantovani A. Zoonoses in the Mediterranean region. Ann Ist Super Sanita. 2006; 42(4): 437-45[PubMed]
  • 7. Pappas G, Memish ZA. Brucellosis in the middle East: a persistent medical, socioeconomic and political issue. J Chemother. 2007; 19(3): 243-8[PubMed]
  • 8. Li W, Raoult D, Fournier PE. Bacterial strain typing in the genomic era. FEMS Microbiol Rev. 2009; 33(5): 892-916[DOI][PubMed]
  • 9. Nagalingam M, Shome R, Balamurugan V, Shome BR, NarayanaRao K, et al. Molecular typing of Brucella species isolates from livestock and human. Trop Anim Health Prod. 2012; 44(1): 5-9[DOI][PubMed]
  • 10. Cerekci A, Kilic S, Bayraktar M, Uyanik MH, Yasar E, Esen B. [Comparison of conventional methods and real-time multiplex polymerase chain reaction for identification and typing of Brucella isolates of human origin]. Mikrobiyol Bul. 2011; 45(3): 392-400[PubMed]
  • 11. Wattiau P, Whatmore AM, Van Hessche M, Godfroid J, Fretin D. Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species. Appl Environ Microbiol. 2011; 77(18): 6674-9[DOI][PubMed]
  • 12. Garcia-Yoldi D, Marin CM, Lopez-Goni I. Restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family of Brucella spp. FEMS Microbiol Lett. 2005; 245(1): 79-84[DOI][PubMed]
  • 13. Lopez-Goni I, Garcia-Yoldi D, Marin CM, de Miguel MJ, Munoz PM, Blasco JM, et al. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol. 2008; 46(10): 3484-7[DOI][PubMed]
  • 14. Cloeckaert A, Vizcaino N, Paquet JY, Bowden RA, Elzer PH. Major outer membrane proteins of Brucella spp.: past, present and future. Vet Microbiol. 2002; 90(1-4): 229-47[PubMed]
  • 15. Cloeckaert A, Verger JM, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology. 1995; 141 ( Pt 9): 2111-21[PubMed]
  • 16. Al Dahouk S, Tomaso H, Prenger-Berninghoff E, Splettstoesser WD, Scholz HC, Neubauer H. Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Crit Rev Microbiol. 2005; 31(4): 191-6[DOI][PubMed]
  • 17. Ancora M, De Santis P, Di Giannatale E, Luciani M, Alessiani A. Molecular typing of Brucella field strains isolated in Italy. Vet Ital. 2005; 41(1): 51-5[PubMed]
  • 18. Queipo-Ortuno MI, Morata P, Ocon P, Manchado P, Colmenero JD. Rapid diagnosis of human brucellosis by peripheral-blood PCR assay. J Clin Microbiol. 1997; 35(11): 2927-30[PubMed]
  • 19. Mirnejad R, Doust RH, Kachuei R, Mortazavi SM, Khoobdel M, Ahamadi A. Simultaneous detection and differentiates of Brucella abortus and Brucella melitensis by combinatorial PCR. Asian Pac J Trop Med. 2012; 5(1): 24-8[DOI][PubMed]
  • 20. Vizcaino N, Kittelberger R, Cloeckaert A, Marin CM, Fernandez-Lago L. Minor nucleotide substitutions in the omp31 gene of Brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other Brucella species. Infect Immun. 2001; 69(11): 7020-8[DOI][PubMed]
  • 21. Da Costa M, Guillou JP, Garin-Bastuji B, Thiebaud M, Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol. 1996; 81(3): 267-75[PubMed]
  • 22. Salehi M, Pishva E, Salehi R, Rahmani R. Isolation of Brucella abortus using PCR-RFLP analysis. Iran J Pub Health. 2006; 35(4)
  • 23. Najafi N, Ghassemian R, Davoody AR, Tayebi A. An unusual complication of a common endemic disease: clinical and laboratory aspects of patients with brucella epididymoorchitis in the north of Iran. BMC Res Notes. 2011; 4: 286[DOI][PubMed]
  • 24. Shareef JM. A review of serological investigations of brucellosis among farm animals and humans in northern provinces of iraq (1974-2004). J Vet Med B Infect Dis Vet Public Health. 2006; 53 Suppl 1: 38-40[DOI][PubMed]
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