Jundishapur Journal of Microbiology

Published by: Kowsar

Molecular Cloning and Expression of Bovine Viral Diarrhea Virus Nonstructural Protein 3 in Escherichia coli

Pezhman Mahmoodi 1 , Masoud Reza Seyfi Abad Shapouri 1 , * , Masoud Ghorbanpour 1 , Mohammad Rahim Haji Hajikolaei 2 , Mohsen Lotfi 3 and Maryam Ekhtelat 1
Authors Information
1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
3 Razi Vaccine and Serum Research Institute, Karaj, IR Iran
Article information
  • Jundishapur Journal of Microbiology: September 01, 2013, 6 (7); e7044
  • Published Online: September 1, 2013
  • Article Type: Research Article
  • Received: July 1, 2012
  • Revised: August 25, 2012
  • Accepted: September 11, 2012
  • DOI: 10.5812/jjm.7044

To Cite: Mahmoodi P, Seyfi Abad Shapouri M R, Ghorbanpour M, Haji Hajikolaei M R, Lotfi M, et al. Molecular Cloning and Expression of Bovine Viral Diarrhea Virus Nonstructural Protein 3 in Escherichia coli, Jundishapur J Microbiol. 2013 ; 6(7):e7044. doi: 10.5812/jjm.7044.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Collett MS, Larson R, Belzer SK, Retzel E. Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology. 1988; 165(1): 200-8[PubMed]
  • 2. MacLachlan NJ, Dubovi EJ. Fenner's Veterinary Virology. 2010;
  • 3. Fredriksen B, Press CM, Loken T, Odegaard SA. Distribution of viral antigen in uterus, placenta and foetus of cattle persistently infected with bovine virus diarrhoea virus. Vet Microbiol. 1999; 64(2-3): 109-22[PubMed]
  • 4. Greiser-Wilke I, Grummer B, Moennig V. Bovine viral diarrhoea eradication and control programmes in Europe. Biologicals. 2003; 31(2): 113-8[PubMed]
  • 5. Presi P, Heim D. BVD eradication in Switzerland--a new approach. Vet Microbiol. 2010; 142(1-2): 137-42[DOI][PubMed]
  • 6. Collett MS, Wiskerchen M, Welniak E, Belzer SK. Bovine viral diarrhea virus genomic organization. Arch Virol Suppl. 1991; 3: 19-27[PubMed]
  • 7. Chimeno Zoth S, Taboga O. Multiple recombinant ELISA for the detection of bovine viral diarrhoea virus antibodies in cattle sera. J Virol Methods. 2006; 138(1-2): 99-108[DOI][PubMed]
  • 8. Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 1989; 17(12): 4713-30[PubMed]
  • 9. Wiskerchen M, Collett MS. Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing. Virology. 1991; 184(1): 341-50[PubMed]
  • 10. Agapov EV, Murray CL, Frolov I, Qu L, Myers TM, Rice CM. Uncleaved NS2-3 is required for production of infectious bovine viral diarrhea virus. J Virol. 2004; 78(5): 2414-25[PubMed]
  • 11. Bolin SR, Ridpath JF. Specificity of neutralizing and precipitating antibodies induced in healthy calves by monovalent modified-live bovine viral diarrhea virus vaccines. Am J Vet Res. 1989; 50(6): 817-21[PubMed]
  • 12. Donis RO, Corapi WV, Dubovi EJ. Bovine viral diarrhea virus proteins and their antigenic analyses. Arch Virol Suppl. 1991; 3: 29-40[PubMed]
  • 13. Sandvik T. Laboratory diagnostic investigations for bovine viral diarrhoea virus infections in cattle. Vet Microbiol. 1999; 64(2-3): 123-34[PubMed]
  • 14. Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine. 2007; : 1248-1249
  • 15. Deregt D, Dubovi EJ, Jolley ME, Nguyen P, Burton KM, Gilbert SA. Mapping of two antigenic domains on the NS3 protein of the pestivirus bovine viral diarrhea virus. Vet Microbiol. 2005; 108(1-2): 13-22[DOI][PubMed]
  • 16. Lecomte C, Pin JJ, De Moerlooze L, Vandenbergh D, Lambert AF, Pastoret PP, et al. ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies. Vet Microbiol. 1990; 23(1-4): 193-201[PubMed]
  • 17. Reddy JR, Kwang J, Okwumabua O, Kapil S, Loughin TM, Lechtenberg KF, et al. Application of recombinant bovine viral diarrhea virus proteins in the diagnosis of bovine viral diarrhea infection in cattle. Vet Microbiol. 1997; 57(2-3): 119-33[PubMed]
  • 18. Vanderheijden N, De Moerlooze L, Vandenbergh D, Chappuis G, Renard A, Lecomte C. Expression of the bovine viral diarrhoea virus Osloss p80 protein: its use as ELISA antigen for cattle serum antibody detection. J Gen Virol. 1993; 74 ( Pt 7): 1427-31[PubMed]
  • 19. Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999; 8(8): 1668-74[DOI][PubMed]
  • 20. Sachdev D, Chirgwin JM. Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin. Protein Expr Purif. 1998; 12(1): 122-32[DOI][PubMed]
  • 21. Chung CT, Niemela SL, Miller RH. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A. 1989; 86(7): 2172-5[PubMed]
  • 22. Haji Hajikolaei MR, Seyfi Abad Shapouri MR. [Serological study of bovine viral diarrhea virus infection of cattle in Ahwaz]. J Vet Res. 2007; 62(1): 21-26
  • 23. Grassmann CW, Isken O, Behrens SE. Assignment of the multifunctional NS3 protein of bovine viral diarrhea virus during RNA replication: an in vivo and in vitro study. J Virol. 1999; 73(11): 9196-205[PubMed]
  • 24. Xu J, Mendez E, Caron PR, Lin C, Murcko MA, Collett MS, et al. Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication. J Virol. 1997; 71(7): 5312-22[PubMed]
  • 25. Moormann RJ, Warmerdam PA, van der Meer B, Schaaper WM, Wensvoort G, Hulst MM. Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein E1. Virology. 1990; 177(1): 184-98[PubMed]
  • 26. Collett MS, Moennig V, Horzinek MC. Recent advances in pestivirus research. J Gen Virol. 1989; 70 ( Pt 2): 253-66[PubMed]
  • 27. Donis RO, Dubovi EJ. Molecular specificity of the antibody responses of cattle naturally and experimentally infected with cytopathic and noncytopathic bovine viral diarrhea virus biotypes. Am J Vet Res. 1987; 48(11): 1549-54[PubMed]
  • 28. Howard CJ, Clarke MC, Brownlie J. An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle sera. Vet Microbiol. 1985; 10(4): 359-69[PubMed]
  • 29. Justewicz DM, Magar R, Marsolais G, Lecomte J. Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera. Vet Immunol Immunopathol. 1987; 14(4): 377-84[PubMed]
  • 30. Beaudeau F, Belloc C, Seegers H, Assie S, Sellal E, Joly A. Evaluation of a blocking ELISA for the detection of bovine viral diarrhoea virus (BVDV) antibodies in serum and milk. Vet Microbiol. 2001; 80(4): 329-37[PubMed]
  • 31. Bhatia S, Sood R, Mishra N, Pattnaik B, Pradhan HK. Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes. Res Vet Sci. 2008; 85(1): 39-45[DOI][PubMed]
  • 32. Neissi A, Seyfi M, Ghorbanpoor M, Jaydary A. Using Rice Flour for Purification of Maltose Binding Fusion Proteins Expressed in E.coli. Jundishapur J Microbiol. 2013; 6(3): 233-6
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments