Jundishapur Journal of Microbiology

Published by: Kowsar

Evaluation of Spirulina platensis Resistance to Different Antibiotics to Find a Selectable Marker for Genetic Transformation

Yasin Moazez 1 , 4 , Hamid Rajabi Memari 1 , * , Hamideh Ofoghi 2 , Mohammad Roayaei 3 and Dariush Nabati Ahmadi 1
Authors Information
1 Agronomy and Plant Breeding Department, Faculty of agriculture, Shahid Chamran University, Ahvaz, IR Iran
2 Medical Biotechnology and Pharmaceutical Industries Department, Iranian Research Organization for Science and Technology, Tehran, IR Iran
3 Biology Department, Shahid Chamran University, Ahvaz, IR Iran
4 Center of Advanced Research and Development of Elite Affairs, ETKA, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: September 01, 2013, 6 (7); e5456
  • Published Online: September 1, 2013
  • Article Type: Research Article
  • Received: June 8, 2012
  • Revised: November 9, 2012
  • Accepted: December 15, 2012
  • DOI: 10.5812/jjm.5456

To Cite: Moazez Y, Rajabi Memari H, Ofoghi H, Roayaei M, Nabati Ahmadi D. Evaluation of Spirulina platensis Resistance to Different Antibiotics to Find a Selectable Marker for Genetic Transformation, Jundishapur J Microbiol. 2013 ; 6(7):e5456. doi: 10.5812/jjm.5456.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Bateman JM, Purton S. Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Molec General Gen. 2000; 263(3): 404-410
  • 2. Brennan Liam, Owende Philip. Biofuels from microalgae. A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable Sustain Energy Rev. 2010; 14(2): 557-577
  • 3. Chen B, Zhan TZ, Lian ZY, Zhang DY. Magnetization of microorganism cells by sol-gel method. Technol Sci. 2008; 51(5): 591-597
  • 4. Andersen DC, Krummen L. Recombinant protein expression for therapeutic applications. Curr Opin Biotechnol. 2002; 13(2): 117-123
  • 5. Contreras-Martel C, Matamala A, Bruna C, Poo-Caamano G, Almonacid D, Figueroa M, et al. The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex. Biophys Chem. 2007; 125(2-3): 388-96[DOI][PubMed]
  • 6. Cao J, Xu Z, Qiu G, Li B. Studies on the sensitivity of Spirulina platensis to antibiotics and herbicide: relationship with selectable markers for genetic transformation. Bioresou technol. 1999; 70(1): 89-93
  • 7. Coll JM. Review. Methodologies for transferring DNA into eukaryotic microalgae. Spanish J Agr Res. 2006; 4(4): 316-330
  • 8. Ahsan M, Habib B, Parvin M, Huntington TC, Hasan MR. A review on culture, production and use of spirulina as food for humans and feeds for domestic animals. 2008;
  • 9. Amaro HM, Guedes A, Malcata FX. Advances and perspectives in using microalgae to produce biodiesel. Appl Energy. 2011; 88(10): 3402-3410
  • 10. Amin S. Review on biofuel oil and gas production processes from microalgae. Energy Conversion Managem. 2009; 50(7): 1834-1840
  • 11. Belay A, Kato T, Ota Y. Spirulina (Arthrospira): potential application as an animal feed supplement. J Appl Phycol. 1996; 8(4-5): 303-311
  • 12. Börner T, Dittmann E. Molecular biology of cyanobacterial toxins. Harmful cyanobacteria. 2005; : 25-40
  • 13. Ciferri O. Spirulina, the edible microorganism. Microbiol Rev. 1983; 47(4): 551-78[PubMed]
  • 14. Brown MR. Nutritional value and use of microalgae in aquaculture. Avances en Nutrición Acuícola VI. Memorias del VI Simposium Internacional de Nutrición Acuícola. 2002; 3: 281-292
  • 15. Celekli A, Yavuzatmaca M, Bozkurt H. Modeling of biomass production by Spirulinaplatensis as function of phosphate concentrations and pH regimes. Bioresour Technol. 2009; 100(14): 3625-9[DOI][PubMed]
  • 16. Chaiklahan R, Chirasuwan N, Siangdung W, Paithoonrangsarid K, Bunnag B. Cultivation of Spirulina platensis using pig wastewater in a semi-continuous process. J Microbiol Biotechnol. 2010; 20(3): 609-14[PubMed]
  • 17. Colla LM, Bertolin TE, Costa JA. Fatty acids profile of Spirulina platensis grown under different temperatures and nitrogen concentrations. Z Naturforsch C. 2004; 59(1-2): 55-9[PubMed]
  • 18. Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, et al. The Firmicutes. Bergey's Manual of Systematic Bacteriology. 2009;
  • 19. Bury NR, Codd GA, Wendelaaar Bonga SE, Flik G. Fatty acids from the cyanobacterium Microcystis aeruginosa with potent inhibitory effects on fish gill Na+/K+-ATPase activity. J Exp Biol. 1998; 201: 81-9[PubMed]
  • 20. Bermejo P, Piñero E, Villar ÁMª. Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chem. 2008; 110(2): 436-445
  • 21. Campbell P, Maranto G, Cantor CR, Glantz LH. Gene Therapy: Legal, Financial and Ethical Issues. BUJ Sci Tech L. 1998; 4: 110
  • 22. Caudales R, Wells JM, Butterfield JE. Cellular fatty acid composition of cyanobacteria assigned to subsection II, order Pleurocapsales. Int J Syst Evol Microbiol. 2000; 50 Pt 3: 1029-34[PubMed]
  • 23. Sharma D, Cukras AR, Rogers EJ, Southworth DR, Green R. Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome. J Mol Biol. 2007; 374(4): 1065-76[DOI][PubMed]
  • 24. Singh N, Poonia S, Maurya AK, Singh DP. Antibiotic sensitivity assay for Spirulina: in relation to marker selection for genetic improvement. Int J Plant Sci (Muzaffarnagar). 2009; 4(2): 403-408
  • 25. Wang H. The C-Phycocyanin/Beta Protein Inhibits Cancer Cell Proliferation. 2008;
  • 26. Blowers AD, Bogorad L, Shark KB, Sanford JC. Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome. Plant Cell Online. 1989; 1(1): 123-132
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments