Jundishapur Journal of Microbiology

Published by: Kowsar

Molecular Cloning and Expression of the Leishmania infantum KMP-11 Gene

Saied Soflaei 1 , Abdolhossein Dalimi 1 , * and Fatemeh Ghafarifar 1
Authors Information
1 Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: March 01, 2013, 6 (2); e4798
  • Published Online: March 2, 2013
  • Article Type: Research Article
  • Received: March 13, 2012
  • Revised: May 22, 2012
  • Accepted: May 27, 2012
  • DOI: 10.5812/jjm.4798

To Cite: Soflaei S, Dalimi A, Ghafarifar F. Molecular Cloning and Expression of the Leishmania infantum KMP-11 Gene, Jundishapur J Microbiol. 2013 ; 6(2):e4798. doi: 10.5812/jjm.4798.

Copyright © 2013, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Material and Methods
4. Results
5. Discussion
  • 1. Alexander J, Satoskar AR, Russell DG. Leishmania species: models of intracellular parasitism. J Cell Sci. 1999; 112: 2993-3002[PubMed]
  • 2. Gangneux JP, Sulahian A, Garin YJ, Farinotti R, Derouin F. Therapy of visceral leishmaniasis due to Leishmania infantum: experimental assessment of efficacy of AmBisome. Antimicrob Agents Chemother. 1996; 40(5): 1214-8[PubMed]
  • 3. Pintado V, artin-Rabadan P, Rivera ML, Moreno S, Bouza E. Visceral leishmaniasis in human immunodeficiency virus (HIV)-infected and non-HIV-infected patients. A comparative study. Medicine (Baltimore). 2001; 80(1): 54-73[DOI]
  • 4. Sundar S, More DK, Singh MK, Singh VP, Sharma S, Makharia A, et al. Failure of pentavalent antimony in visceral leishmaniasis in India: report from the center of the Indian epidemic. Clin Infect Dis. 2000; 31(4): 1104-7[DOI][PubMed]
  • 5. Dondji B, Perez-Jimenez E, Goldsmith-Pestana K, Esteban M, McMahon-Pratt D. Heterologous prime-boost vaccination with the LACK antigen protects against murine visceral leishmaniasis. Infect Immun. 2005; 73(8): 5286-9[DOI][PubMed]
  • 6. Shaddel M, Oormazdi H, Akhlaghi L, Kazemi B, Bandehpour M. Cloning of Leishmania Major P4 Gene Yahkhteh Med J. 2008; 10(3): 201-4
  • 7. Sujata K, Soong L, Colmenares M, Gold Smith K, Mc Mahon-Pratt D. The immunologically protective P4 Antigen of leishmania amastigotes. J Biol Chem. 2000; 275: 37789-97
  • 8. Tabatabaie F, Dalimi A, Sharifi Z, Hoseini AZ. Cloning and sequencing of Leishmania major thiol-specific-antioxidant antigen (TSA) gene. Iranian J Parasitol. 2007; 2(4): 30-41
  • 9. Tabatabaie F, Ghaffarifar F, Dalimi A, Sharifi Z, Hassan Z. Enhancement of cellular and antibody immune response to Leishmania major. Biochem Cell Arch. 2008; 8(1): 35-43
  • 10. Berberich C, Machado G, Morales G, Carrillo G, Jimenez-Ruiz A, Alonso C. The expression of the Leishmania infantum KMP-11 protein is developmentally regulated and stage specific. Biochim Biophys Acta. 1998; 1442: 230-7[DOI]
  • 11. Stebeck CE, Baron GS, Beecroft RP, Pearson TW. Molecular characterization of the kinetoplastid membrane protein-11 from African trypanosomes. Mol Biochem Parasitol. 1996; 81(1): 81-8[DOI]
  • 12. Jardim A, Hanson S, Ullman B, McCubbin WD, Kay CM, Olafson RW. Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11. Biochem J. 1995; 305: 315-20[PubMed]
  • 13. Ramirez JR, Berberich C, Jaramillo A, Alonso C, Velez IV. Molecular and antigenic characterization of the Leishmania (Viannia) panamensis kinetoplastid membrane protein-11. Mem Inst Oswaldo Cruz. 1998; 93(2): 247-54[DOI][PubMed]
  • 14. Fuertes MA, Perez JM, Soto M, Lopez MC, Alonso C. calcium-induced conformational changes in Leishmania infantum kinetoplastid membrane protein-11. J Biol Inorg Chem. 2001; 6(1): 107-17[DOI][PubMed]
  • 15. Jensen AT, Gasim S, Ismail A, Gaafar A, Kurtzhals JA, Kemp M, et al. Humoral and cellular immune responses to synthetic peptides of the Leishmania donovani kinetoplastid membrane protein-11. Scand J Immunol. 1998; 48(1): 103-9[DOI][PubMed]
  • 16. Tolson DL, Jardim A, Schnur LF, Stebeck C, Tuckey C, Beecroft RP, et al. The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation. Infect Immun. 1994; 62(11): 4893-9[PubMed]
  • 17. Basu R, Bhaumik S, Basu JM, Naskar K, De T, Roy S. Kinetoplastid membrane protein-11 DNA vaccination induces complete protection against both pentavalent antimonial-sensitive and -resistant strains of Leishmania donovani that correlates with inducible nitric oxide synthase activity and IL-4 generation: evidence for mixed Th1- and Th2-like responses in visceral leishmaniasis. J Immunol. 2005; 174(11): 7160-71[PubMed]
  • 18. Trujillo C, Ramirez R, Velez ID, Berberich C. The humoral immune response to the kinetoplastid membrane protein-11 in patients with American leishmaniasis and Chagas disease: prevalence of IgG subclasses and mapping of epitopes. Immunol Lett. 1999; 70(3): 203-9[DOI]
  • 19. Maranon C, Thomas MC, Planelles L, Lopez MC. The immunization of A2/K(b) transgenic mice with the KMP11-HSP70 fusion protein induces CTL response against human cells expressing the T. cruzi KMP11 antigen: identification of A2-restricted epitopes. Mol Immunol. 2001; 38(4): 279-87[DOI]
  • 20. Planelles L, Thomas M, Pulgar M, Maranon C, Grabbe S, Lopez MC. Trypanosoma cruzi heat-shock protein-70 kDa,alone or fused to the parasite KMP11 antigen, induces functional maturation of murine dendritic cells. Immunol Cell Biol. 2002; 80(3): 241-7[DOI][PubMed]
  • 21. Ramirez JR, Gilchrist K, Robledo S, Sepulveda JC, Moll H, Soldati D, et al. Attenuated Toxoplasma gondii ts-4 mutants engineered to express the Leishmania antigen KMP-11 elicit a specific immune response in BALB/c mice. Vaccine. 2001; 20(3-4): 455-61[DOI]
  • 22. Berberich C, Ramirez-Pineda JR, Hambrecht C, Alber G, Skeiky YA, Moll H. Dendritic cell (DC)-based protection against an intracellular pathogen is dependent upon DC-derived IL-12 and can be induced by molecularly defined antigens. J Immunol. 2003; 170(6): 3171-9[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments